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1. 发明申请

WO2004039947A3 DNA POLYMERASE MUTANTS WITH REVERSE TRANSCRIPTASE ACTIVITY 审中-公开
标题翻译：具有反向转录酶活性的DNA聚合酶突变体
公开(公告)号：WO2004039947A3
公开(公告)日：2006-10-12
申请号：PCT/US0325762
申请日：2003-08-15
申请人： STRATAGENE INC , AREZI BAHRAM , HOGREFE HOLLY , SORGE JOSEPH A , HANSEN CONNIE JO
发明人： AREZI BAHRAM , HOGREFE HOLLY , SORGE JOSEPH A , HANSEN CONNIE JO
IPC分类号： C12N9/12 , C07H21/04 , C12Q1/48
CPC分类号： C12N9/1276 , B82Y5/00 , B82Y10/00 , C12N9/1252
摘要： The present invention relates to compositions and kits comprising a mutant DNA polymerase with increased reverse transcriptase activity. The invention also relates to methods for using the subject compositions and kits.
摘要翻译：本发明涉及包含具有增加的逆转录酶活性的突变型DNA聚合酶的组合物和试剂盒。本发明还涉及使用本发明组合物和试剂盒的方法。

2. 发明申请

WO0244326A2 METHODS FOR DETECTION OF A TARGET NUCLEIC ACID BY CAPTURE 审中-公开
标题翻译：通过捕获来检测目标核酸的方法
公开(公告)号：WO0244326A2
公开(公告)日：2002-06-06
申请号：PCT/US0144215
申请日：2001-11-26
申请人： STRATAGENE INC , SORGE JOSEPH A , WHALEN ANNE M
发明人： SORGE JOSEPH A , WHALEN ANNE M
IPC分类号： C12Q1/68 , G01N33/48 , G01N33/50 , G06F19/00 , C12N
CPC分类号： C12Q1/6823 , C12Q1/6816 , C12Q2521/301 , C12Q2531/113 , C12Q2521/307 , C12Q2525/161 , C12Q2525/301 , C12Q2561/109 , C12Q2565/519 , C12Q2561/101 , C12Q2521/107
摘要： The invention relates to a method of generating a signal indicative of the presence of a target nucleic acid in a sample, where the method includes forming a cleavage structure by incubating a sample comprising a target nucleic acid with a probe having a secondary structure that changes upon binding of the probe to the target nucleic acid and further comprising a binding moiety. The invention also includes the steps of cleaving the cleavage structure with a nuclease to release a nucleic acid fragment to generate a signal that is indicative of the presence of a target nucleic acid in a sample, and detecting and/or measuring the amount of the fragment captured by binding of a binding moiety to a capture element on a solid support.
摘要翻译：本发明涉及产生指示样品中目标核酸存在的信号的方法，其中该方法包括通过将包含靶核酸的样品与具有二级结构变化的探针一起孵育形成切割结构探针与靶核酸的结合并且还包含结合部分。本发明还包括用核酸酶切割切割结构以释放核酸片段以产生指示样品中目标核酸存在的信号以及检测和/或测量片段的量的步骤通过结合部分与固体支持物上的捕获元件的结合捕获。

3. 发明申请

WO0238729A9 GENE MONITORING AND GENE IDENTIFICATION USING cDNA ARRAYS 审中-公开
标题翻译：使用cDNA序列进行基因监测和基因鉴定
公开(公告)号：WO0238729A9
公开(公告)日：2003-05-30
申请号：PCT/US0145555
申请日：2001-11-01
申请人： STRATAGENE INC , SORGE JOSEPH A
发明人： SORGE JOSEPH A
IPC分类号： C12Q1/68 , C12M1/34
CPC分类号： C12Q1/6809 , C12Q2565/501 , C12Q2525/173
摘要： cDNA microarrays are provided for increasing the accuracy and reliability of expression profiling techniques and for identifying new genes. An array comprises a plurality of nucleic acid members, each member having a unique position and stably associated with a solid support. Each nucleic acid member comprises a noncoding sequence present at either the 3'-end or the 5'-end of an RNA transcript (e.g., such as an untranslated region or UTR). In one embodiment, each nucleic acid member is less than 1000 nucleotides. In another embodiment, each nucleic acid member is elss than 600 nucleotides. In a further embodiment of the invention, each nucleic acid member comprises substantially noncoding sequences.
摘要翻译：提供cDNA微阵列以提高表达谱分析技术的准确性和可靠性，并确定新基因。阵列包括多个核酸构件，每个构件具有独特的位置并且与固体支持物稳定相关。每个核酸成员包括存在于RNA转录物（例如非翻译区或UTR）的3'末端或5'末端的非编码序列。在一个实施方案中，每个核酸成员小于1000个核苷酸。在另一个实施方案中，每个核酸成员都是比600个核苷酸更精细的。在本发明的另一个实施方案中，每个核酸成员包含基本上非编码序列。

4. 发明申请

WO0238729A3 GENE MONITORING AND GENE IDENTIFICATION USING cDNA ARRAYS 审中-公开
标题翻译：利用cDNA阵列进行基因监测和基因鉴定
公开(公告)号：WO0238729A3
公开(公告)日：2002-07-25
申请号：PCT/US0145555
申请日：2001-11-01
申请人： STRATAGENE INC , SORGE JOSEPH A
发明人： SORGE JOSEPH A
IPC分类号： C12Q1/68 , C12M1/34
CPC分类号： C12Q1/6809 , C12Q2565/501 , C12Q2525/173
摘要： cDNA microarrays are provided for increasing the accuracy and reliability of expression profiling techniques and for identifying new genes. An array comprises a plurality of nucleic acid members, each member having a unique position and stably associated with a solid support. Each nucleic acid member comprises a noncoding sequence present at either the 3'-end or the 5'-end of an RNA transcript (e.g., such as an untranslated region or UTR). In one embodiment, each nucleic acid member is less than 1000 nucleotides. In another embodiment, each nucleic acid member is elss than 600 nucleotides. In a further embodiment of the invention, each nucleic acid member comprises substantially noncoding sequences.
摘要翻译：提供cDNA微阵列用于提高表达谱分析技术的准确性和可靠性，并用于鉴定新基因。阵列包含多个核酸成员，每个成员具有独特的位置并与固体支持物稳定结合。每个核酸成员包含存在于RNA转录物的3'端或5'端的非编码序列（例如非翻译区或UTR）。在一个实施方案中，每个核酸成员少于1000个核苷酸。在另一个实施方案中，每个核酸成员大约600个核苷酸。在本发明的另一个实施方案中，每个核酸成员包含基本上非编码序列。

5. 发明申请

WO0132922A3 METHODS FOR DETECTION OF A TARGET NUCLEIC ACID SEQUENCE 审中-公开
标题翻译：检测目标核酸序列的方法
公开(公告)号：WO0132922A3
公开(公告)日：2002-06-06
申请号：PCT/US0029663
申请日：2000-10-27
申请人： STRATAGENE INC
发明人： SORGE JOSEPH A
IPC分类号： C12N15/09 , C12N9/12 , C12N9/22 , C12Q1/68
CPC分类号： C12Q1/686 , C12Q1/6823 , Y10S435/81 , Y10S435/822 , Y10T436/143333 , C12Q2561/109 , C12Q2531/113
摘要： The invention relates to a method of generating a signal indicative of the presence of a target nucleic acid sequence in a sample, where the method includes forming a cleavage structure by incubating a sample containing a target nucleic acid sequence with a nucleic acid polymerase and cleaving the cleavage structure with an FEN nuclease to generate a cleaved nucleic acid fragment. The invention also relates to methods of detecting or measuring a target nucleic acid sequence, where the method includes forming a cleavage structure by incubating a target nucleic acid sequence with a nucleic acid polymerase, cleaving the cleavage structure with an FEN nuclease and detecting or measuring the release of a fragment.
摘要翻译：本发明涉及一种产生指示样品中目标核酸序列存在的信号的方法，其中该方法包括通过将含有靶核酸序列的样品与核酸聚合酶一起孵育而形成切割结构，用FEN核酸酶切割结构以产生切割的核酸片段。本发明还涉及检测或测量靶核酸序列的方法，其中该方法包括通过将靶核酸序列与核酸聚合酶孵育形成切割结构，用FEN核酸酶切割切割结构，并检测或测量释放片段。

6. 发明申请

WO2006102309A3 METHODS, COMPOSITIONS, AND KITS FOR DETECTION OF MICRO RNA 审中-公开
标题翻译：用于检测微RNA的方法，组合物和试剂盒
公开(公告)号：WO2006102309A3
公开(公告)日：2008-01-24
申请号：PCT/US2006010193
申请日：2006-03-21
申请人： STRATAGENE INC , SORGE JOSEPH A , MULLINAX REBECCA L
发明人： SORGE JOSEPH A , MULLINAX REBECCA L
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6809 , C12Q1/6816 , C12Q2533/107 , C12Q2545/114 , C12Q2525/207
摘要： The present invention provides methods, nucleic acids, compositions, and kits for detecting microRNA (miRNA) in samples. The methods comprise ligating two oligonucleotides together in an miRNA mediated fashion, and detection of the ligation product. The methods can further comprise amplification of the ligation product, such as by PCR. The nucleic acids, compositions, and kits typically comprise some or all of the components necessary to practice the method of the invention.
摘要翻译：本发明提供了用于检测样品中的微小RNA（miRNA）的方法，核酸，组合物和试剂盒。所述方法包括以miRNA介导的方式将两个寡核苷酸结合在一起，并检测连接产物。所述方法可以进一步包括连接产物的扩增，例如通过PCR扩增。核酸，组合物和试剂盒通常包括实施本发明方法所需的一些或全部成分。

7. 发明申请

WO2004081182A3 COMPOSITIONS AND METHODS FOR POLYNUCLEOTIDE SEQUENCE DETECTION 审中-公开
标题翻译：多核苷酸序列检测的组合物和方法
公开(公告)号：WO2004081182A3
公开(公告)日：2006-06-15
申请号：PCT/US2004006948
申请日：2004-03-05
申请人： STRATAGENE INC , SORGE JOSEPH A , FIRMIN ANDREW
发明人： SORGE JOSEPH A , FIRMIN ANDREW
IPC分类号： C12N20060101 , C12N1/00 , C12Q1/68
CPC分类号： C12Q1/6818 , B82Y5/00 , B82Y10/00 , C12Q1/6827 , C12Q2565/1015 , C12Q2535/125
摘要： The present invention provides compositions, kits, and methods for detecting polynucleotide sequence differences. The method involves amplifying a polynucleotide in the presence of a labeled nucleotide whose incorporation into the amplified product can indicate the presence of a sequence difference within the polynucleotide template. The invention is particularly useful for differentiating two or more closely related polynucleotide sequences, for example, in determining which allele or alleles of a multiallelic organism are present in a target polynucleotide.
摘要翻译：本发明提供了用于检测多核苷酸序列差异的组合物，试剂盒和方法。该方法包括在标记核苷酸存在下扩增多核苷酸，其掺入到扩增产物中可以指示在多核苷酸模板内存在序列差异。本发明特别可用于区分两个或更多个密切相关的多核苷酸序列，例如在确定多核生物的等位基因或等位基因存在于靶多核苷酸中。

8. 发明申请

WO03054139B1 COMPOSITIONS AND METHODS UTILIZING DNA POLYMERASES 审中-公开
标题翻译：组合物和利用DNA聚合物的方法
公开(公告)号：WO03054139B1
公开(公告)日：2004-02-05
申请号：PCT/US0220562
申请日：2002-06-27
申请人： STRATAGENE INC , SORGE JOSEPH A , HANSEN CONNI JO , HOGREFE HOLLY
发明人： SORGE JOSEPH A , HANSEN CONNI JO , HOGREFE HOLLY
IPC分类号： G01N33/53 , C12N9/12 , C12N15/09 , C12Q1/25 , C12Q1/48 , C12Q1/68 , G01N33/566 , G01N33/58 , C12P19/34
CPC分类号： B82Y5/00 , B82Y10/00 , C12N9/1252 , C12N9/1276
摘要： The invention features a novel isolated Family B DNA polymerase, a Thermococcus polymerase JDF-3, and mutant recombinant forms thereof. Mutant polymerases of the invention are deficient in 3' to 5' exonuclease activity and/or exhibit reduced discrimination against non-conventional nucleotides relative to the wild-type form of the polymerase.
摘要翻译：本发明的特征在于新的分离的家族B DNA聚合酶，热球菌聚合酶JDF-3及其突变体重组形式。本发明的突变型聚合酶相对于野生型形式的聚合酶，缺乏3'至5'核酸外切酶活性和/或表现出对非常规核苷酸的降低的鉴别。

9. 发明申请

WO03016550A3 COMPOSITIONS AND METHODS COMPRISING CONTROL NUCLEIC ACID 审中-公开
标题翻译：包含控制核酸的组合物和方法
公开(公告)号：WO03016550A3
公开(公告)日：2003-07-17
申请号：PCT/US0226157
申请日：2002-08-16
申请人： STRATAGENE INC , SORGE JOSEPH A , MULLINAX REBECCA LYNN , NOVORADOVSKY ALEXEY
发明人： SORGE JOSEPH A , MULLINAX REBECCA LYNN , NOVORADOVSKY ALEXEY
IPC分类号： C12Q1/68 , C07H21/04 , C12P19/34
CPC分类号： C12Q1/6837 , C12Q1/6848 , C12Q2545/101
摘要： The present invention relates, in part, to control nucleic acid molecules having no significant sequence homology to any known nucleic acid, and predefined G/C-content. The present invention further relates to method of using control nucleic acid molecules to validate microarray analyses, compositions comprising control nucleic acid molecules, and kits comprising control nucleic acid molecules.
摘要翻译：本发明部分涉及控制与任何已知核酸无显着序列同源性的核酸分子和预定义的G / C含量。本发明还涉及使用对照核酸分子来验证微阵列分析的方法，包含对照核酸分子的组合物和包含对照核酸分子的试剂盒。

10. 发明申请

WO03054139A2 COMPOSITIONS AND METHODS UTILIZING DNA POLYMERASES 审中-公开
标题翻译：组合物和利用DNA聚合物的方法
公开(公告)号：WO03054139A2
公开(公告)日：2003-07-03
申请号：PCT/US0220562
申请日：2002-06-27
申请人： STRATAGENE INC , SORGE JOSEPH A , HANSEN CONNI JO , HOGREFE HOLLY
发明人： SORGE JOSEPH A , HANSEN CONNI JO , HOGREFE HOLLY
IPC分类号： G01N33/53 , C12N9/12 , C12N15/09 , C12Q1/25 , C12Q1/48 , C12Q1/68 , G01N33/566 , G01N33/58 , C12N
CPC分类号： B82Y5/00 , B82Y10/00 , C12N9/1252 , C12N9/1276
摘要： The invention features a novel isolated Family B DNA polymerase, a Thermococcus polymerase JDF-3, and mutant recombinant forms thereof. Mutant polymerases of the invention are deficient in 3’ to 5’ exonuclease activity and/or exhibit reduced discrimination against non-conventional nucleotides relative to the wild-type form of the polymerase.
摘要翻译：本发明的特征在于一种新型的分离的家族B DNA聚合酶，热球菌聚合酶JDF-3及其突变体重组形式。本发明的突变型聚合酶相对于野生型形式的聚合酶，缺乏3'至5'核酸外切酶活性和/或表现出对非常规核苷酸的降低的鉴别。

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