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    • 6. 发明申请
    • MALDI-MATRIX
    • MALDI MATRIX
    • WO2004023147A2
    • 2004-03-18
    • PCT/EP0308435
    • 2003-07-30
    • NUTRICIA NVSTAHL BERNDBOEHM GUENTHERMANK MARKO
    • STAHL BERNDBOEHM GUENTHERMANK MARKO
    • G01N27/64G01N27/447G01N27/62G01N30/72G01N33/68H01J49/16
    • H01J49/164G01N33/6848G01N33/6851Y10T436/10
    • The invention relates to matrixes for ultraviolet matrix-assisted laser desorption-ionisation mass spectrometry made of the salt of an amine which reacts as a proton acceptor and an organic substance which reacts as a proton donator. Either the amine or the organic substance absorbs UV light. Said matrixes are characterised in that they represent an ionic liquid at room temperature and in that the amine is selected from the group which is made up of 3-aminoquinoline, pyridine, a primary amine whose N-Atom is bound to a phenyl radical or a straight or branched, saturated C1-C11-alkyl radical which can be substituted by an OH-group, a secondary and tertiary amine whose N-Atoms are bound to two or three radicals which can be the same or different and represent a straight or branched, saturated C1-C8-alkyl radical which can be substituted by an OH-group and a phenyl radical, imidazole and the C and/or N-alkylated imidazole derivatives and also characterised in that the organic substance is selected from the group which is made up of 2,5-dihydroxybenzoic acid and the isomers thereof, 2-hydroxy-5-methoxy-benzoic acid and the isomers thereof, picolinic-acid, 3-hydroxypicolinic acid, nicotinic acid, 5-chloro-2-mercapto-benzo-thiazole, 6-aza-2-thiothymine, 2',4',6'-trihydroxyacetophenone-monohydrate, 2',6'-di-hydroxyaceto-phenone, 9H-pyridol[3,4-b]indole, dithranole, trans-3-indolacrylic acid, osazones, ferulic acid, 2,5-dihydroxyacetophenone, 1-nitrocarbazole, 7-amino-4-methylcumarine, 2-(p-hdroxyphenylazo)-benzoic acid, 8-aminopyrene-2,3,4-trissulfonic acid, 2-[2E-3-(4-tert-butylphenyl)-2-methylprop-2-enylidene]malonitril (DCTB), 4-methoxy-3-hydroxycinnamic acid and 3,4-dihydroxycinnamic acid. Said liquid matrixes enable reproducible, error-free analysis values to be obtained. The combination of pure mass spectrometric analysis with additional knowledge of enzymatic reactions/modifications with the possible monitoring is also described.
    • 提供矩阵作为对质子受体和胺作为质子供体反应性有机物质,其特征在于,无论是胺或有机物质吸收的UV光反应的盐的紫外线基质辅助激光解吸电离质谱分析法。 这些矩阵的特征在于,该矩阵代表在室温下的离子液体,使得所述胺选自由3-氨基喹啉,吡啶,伯胺组成的组中,选择在N原子的苯基烷基或直链或支链的, 其可通过一个OH基团取代的饱和的C1-C11的烷基键合,一个仲和叔胺,在N原子,两个或三个基团所连接,其可以是相同的或不同的,并且它们将成为 其可被OH基团取代的直链或支链的,饱和的C1-C8烷基,和苯基是,咪唑和C-和/或N-烷基化的咪唑衍生物和有机物质选自由的组中选择 从2,5-二羟基苯甲酸和它们的异构体,2-羟基-5-甲氧基 - 苯甲酸和它们的异构体,甲基吡啶酸,3-Hydroxypicolin酸,烟酸,5-氯-2- 巯基苯并噻唑,6-氮杂-2-硫代胸腺嘧啶,2 '4' ,6'-三羟基苯乙酮一水合物,2',6'-二 - hydroxyaceto基二苯酮,-9H-吡啶并[3,4-B] 吲哚,蒽三酚,反式-3-吲哚丙烯酸,osazones,阿魏酸,2,5-二羟基苯乙酮,1- nitrocarbazole,7-氨基-4-甲基香豆素,2-(对 - Hdroxyphenylazo)苯甲酸,8-氨基芘-2,3-, 4-trissulfonsäure,2- [2E-3-(4-叔丁基苯基)-2-甲基丙-2-烯亚基]丙二腈(DCTB),4-甲氧基-3-羟基肉桂酸和3,4-二羟基肉桂酸。 与这些液体基质无差错和可再现的分析值可以得到,特别是与酶反应的附加知识纯质谱分析的组合/设置有监测的可能性修改。
    • 8. 发明申请
    • ANTI-INFECTIOUS CARBOHYDRATES
    • 抗感染碳水化合物
    • WO03028738A3
    • 2003-08-21
    • PCT/EP0210761
    • 2002-09-25
    • NUTRICIA NVSTAHL BERNDFINKE BERNDTSCHMITT JOACHIMGOEBEL WERNERSLAGHUIS JOERG
    • STAHL BERNDFINKE BERNDTSCHMITT JOACHIMGOEBEL WERNERSLAGHUIS JOERG
    • A61K31/702A61K31/715A61P31/00
    • A61K31/702A61K31/715
    • The invention relates to the use of neutral straight-chain or branched oligosaccharides for preventing the invasion and infection of mammal cells by pathogens and for fighting diseases caused by such pathogens. The invention also relates to food, dietetic products and pharmaceutical agents containing said oligosaccharides. Said oligosaccharides consist of a base unit of general formula (I), and between 0 and 19 other units of general formula (II), which are linked directly or indirectly to the base unit. In said formulae, Gal represents a galactose monosaccharide unit; Glc represents a glucose monosaccharide unit; HexNAc represents an N-acetylated galactosamine or glucosamine monosaccharide unit (GalNAc or GlcNAc); the radicals R independently represent a beta 1-3 or beta 1-6 glycosidic link to the HexNAc monosaccharide unit of the next [Gal-HexNAc] unit of general formula (II), and on a terminal [Gal-HexNAc] unit, a deoxyhexose radical which is linked to the galactose monosaccharide unit of said terminal [Gal-HexNAc] unit, or said radicals R are not present; and a terminal [Gal-HexNAc] unit, wherein the radical(s) R represent a deoxyhexose radical, can have another deoxyhexose radical on the HexNAc monosaccharide unit.
    • 描述了一种用于预防侵袭和哺乳动物细胞的感染的使用中性直链或支链的寡糖的病原体和用于通过这些疾病病原体的控制而引起的含寡糖-这些食品,营养和药物组合物。 这些寡糖是由下述通式(I)的基本单元及0〜19其它,从而直接或间接连接的下述通式(II)的单元式中,Gal是半乳糖单糖单元,GLC是葡萄糖单糖单元, HexNAc表示N-乙酰半乳糖胺或葡糖胺单糖单元(半乳糖胺或GlcNAc的),残基R各自独立地为1-3的β或β1-6糖苷键到下一[半乳糖的单糖HexNAc单元 HexNAc]通式单元(II),并在终端[Gal一HexNAc]单元代表链接到所述终端[脱氧己残基的半乳糖单糖单元中的半乳糖 - HexNAc]单元或不存在,并 末端[Gal-HexNAc]部分,其中基团R是其中hexNAc单糖单元可以具有另外的脱氧己糖基团的脱氧己糖基团 集。