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1. 发明申请

WO2013029021A1 COMPOSITIONS AND METHODS FOR DETECTION OF MULTIPLE MICROORGANISMS 审中-公开
标题翻译：用于检测多种微生物的组合物和方法
公开(公告)号：WO2013029021A1
公开(公告)日：2013-02-28
申请号：PCT/US2012/052383
申请日：2012-08-24
申请人： LIFE TECHNOLOGIES CORPORATION , TEBBS, Robert , CUMMINGS, Craig , BRZOSKA, Pius , MATHENY, Sharon , O'CONNELL, Catherine , FURTADO, Manohar , FANG, Rixun , PETRAUSKENE, Olga , LIEW, Sueh-Ning , SCHUMAKER, Michael
发明人： TEBBS, Robert , CUMMINGS, Craig , BRZOSKA, Pius , MATHENY, Sharon , O'CONNELL, Catherine , FURTADO, Manohar , FANG, Rixun , PETRAUSKENE, Olga , LIEW, Sueh-Ning , SCHUMAKER, Michael
IPC分类号： C12Q1/68
CPC分类号： C12Q1/686 , C12Q1/689 , C12Q2600/16
摘要： The present teachings describe compositions, methods and kits for detection of one or multiple microorganism contaminants in samples. Some embodiments relate to detecting one or more microorganisms producing virulence factors such as a shiga toxin ( stx ) or an eae . In some embodiments, compositions, methods and kits can detect and identify individual strains and serotypes of shiga toxin producing microorganisms. Some embodiments describe compositions, methods and kits for detecting STEC microbes. Workflows for multiple microbe detection and identification are also described.
摘要翻译：本教导描述了用于检测样品中一种或多种微生物污染物的组合物，方法和试剂盒。一些实施方案涉及检测产生毒力因子（例如志贺毒素（stx）或eae）的一种或多种微生物。在一些实施方案中，组合物，方法和试剂盒可以检测和鉴定产生志贺毒素的微生物的个体菌株和血清型。一些实施方案描述了用于检测STEC微生物的组合物，方法和试剂盒。还描述了用于多个微生物检测和鉴定的工作流程。

2. 发明申请

WO2010111509A1 DISCRIMINATORY POSITIVE/EXTRACTION CONTROL DNA 审中-公开
标题翻译：歧视积极/提取控制DNA
公开(公告)号：WO2010111509A1
公开(公告)日：2010-09-30
申请号：PCT/US2010/028693
申请日：2010-03-25
申请人： LIFE TECHNOLOGIES CORPORATION , BREWER, Michael , PETRAUSKENE, Olga , LIU, Jen-Kuei , CUMMINGS, Craig , LIEW, Sueh-Ning , FURTADO, Manohar
发明人： BREWER, Michael , PETRAUSKENE, Olga , LIU, Jen-Kuei , CUMMINGS, Craig , LIEW, Sueh-Ning , FURTADO, Manohar
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6851 , C12Q2561/107 , C12Q2545/107 , C12Q2537/107
摘要： The present teachings generally relate to methods and kits incorporating a discriminating positive control for determining whether a particular microorganism or group of microorganisms are present in a sample, for example but not limited to a food, environmental, agricultural, biopharmaceutical, pharmaceutical, or water sample. According to certain methods, at least part of a starting material, for example but not limited to, a food, environmental, agricultural, biopharmaceutical, pharmaceutical, or water sample can be combined with a culture medium and incubated under conditions suitable for microbial growth followed by extracting microorganism and added control nucleic acids for analysis. The extracted nucleic acids are amplified and the amplified nucleic acids are detected, directly or indirectly, and the fidelity of the methods and the presence or absence of the corresponding microorganism is determined because the discriminating positive control provides both confirmatory results for the methods used but eliminates false positive results.
摘要翻译：本教导通常涉及包含用于确定特定微生物或一组微生物是否存在于样品中的鉴别性阳性对照的方法和试剂盒，例如但不限于食品，环境，农业，生物制药，药物或水样品。根据某些方法，至少部分原料，例如但不限于食品，环境，农业，生物制药，药物或水样品可以与培养基组合，并在适合于微生物生长的条件下温育通过提取微生物和添加的对照核酸进行分析。所提取的核酸被扩增并且直接或间接检测扩增的核酸，并且确定方法的保真度和相应的微生物的存在或不存在，因为鉴别性阳性对照提供所使用的方法的确认结果但消除假阳性结果。

3. 发明申请

WO2012003412A3 INDUCIBLE NUCLEIC ACID TARGETS FOR DETECTION OF PATHOGENS, METHODS AND COMPOSITIONS THEREOF 审中-公开
公开(公告)号：WO2012003412A3
公开(公告)日：2012-01-05
申请号：PCT/US2011/042723
申请日：2011-06-30
申请人： LIFE TECHNOLOGIES CORPORATION , BALACHANDRAN, Priya , CUMMINGS, Craig , PETRAUSKENE, Olga , PAWAR, Hemant , TEBBS, Robert , FURTADO, Manohar
发明人： BALACHANDRAN, Priya , CUMMINGS, Craig , PETRAUSKENE, Olga , PAWAR, Hemant , TEBBS, Robert , FURTADO, Manohar
IPC分类号： C12Q1/68
摘要： The present application describes compositions, methods and kits for rapid detection and identification of various microorganisms using inducible RNA. Methods for rapidly detecting microorganisms by detecting expression of inducible RNA of target genes following induction of a target gene using an inducer are described. Some embodiments describe methods and workflows for rapidly detecting microbes such as, but not limited to, Salmonella spp, Listeria spp. and Vibrio spp. Compositions and kits comprise primer nucleic acid sequences having hybridization specificity for priming amplification of genes of microorganisms (or gene fragments) that are responsive to one or more RNA-inducing agents. In some embodiments, kits and compositions further comprise probe nucleic acid sequences having hybridization specificity for genes responsive to RNA-inducing agents or fragments thereof.

4. 发明申请

WO2012174119A2 COMPOSITIONS AND METHODS FOR DETECTION OF CRONOBACTER SPP. AND CRONOBACTER SPECIES AND STRAINS 审中-公开
标题翻译：用于检测CRONOBACTER SPP的组合物和方法。和克隆物种和菌株
公开(公告)号：WO2012174119A2
公开(公告)日：2012-12-20
申请号：PCT/US2012/042263
申请日：2012-06-13
申请人： LIFE TECHNOLOGIES CORPORATION , JI, Yongmei , SHIH, Sheung-Mei ("Rita") , DEGORICIJA, Lovorka , ZHOU, Zhaohui , CUMMINGS, Craig , FURTADO, Manohar , BRZOSKA, Pius , BURRELL, Angela , LEONG, Harrison , FANG, Xingwang , BOUNPHENG, Mangkey , SHAH, Rohan
发明人： JI, Yongmei , SHIH, Sheung-Mei ("Rita") , DEGORICIJA, Lovorka , ZHOU, Zhaohui , CUMMINGS, Craig , FURTADO, Manohar , BRZOSKA, Pius , BURRELL, Angela , LEONG, Harrison , FANG, Xingwang , BOUNPHENG, Mangkey , SHAH, Rohan
IPC分类号： C12Q1/68
CPC分类号： C12Q1/689 , C12R1/01
摘要： Disclosed are genomic sequences for nine strains of C ronobacter spp . (C. sakazakii - 696, 701, 680; C. malonaticus - 507, 681; C. turicensis - 564; C. muytjensii - 530; C. dublinensis - 582; C. genomospl - 581) and compositions, methods, and kits for detecting, identifying and distinguishing C ronobacter spp . strains from each other and from non-C ronobacter spp . strains. Some embodiments describe isolated nucleic acid compositions unique to certain C ronobacter strains as well as compositions that are specific to all C ronobacter spp . Primer and probe compositions and methods of use of primers and probes are also provided. Kits for identification of C ronobacter spp . are also described. Some embodiments relate to computer software methods for setting a control based threshold for analysis of PCR
摘要翻译：公开了九个克罗杆菌属菌株的基因组序列。（C. sakazakii-696,701,680; C. malonaticus-507,681; C. turicensis-564; C. muytjensii-530; C.dublinensis-582; C.基因组蛋白-58）和组合物，方法和试剂盒用于检测，识别和鉴别克罗杆菌属。菌株彼此和非克罗杆菌属。株。一些实施方案描述了某些克罗杆菌菌株特有的分离的核酸组合物以及对所有克罗杆菌属特异性的组合物。还提供引物和探针组合物以及引物和探针的使用方法。克隆杆菌属鉴定试剂盒也被描述。一些实施例涉及用于设置用于PCR分析的基于控制的阈值的计算机软件方法

5. 发明申请

WO2012003409A1 DETECTION OF LISTERIA SPECIES IN FOOD AND ENVIRONMENTAL SAMPLES, METHODS AND COMPOSITIONS THEREOF 审中-公开
标题翻译：食品和环境样品中的LISTERIA物种的检测方法及其组合物
公开(公告)号：WO2012003409A1
公开(公告)日：2012-01-05
申请号：PCT/US2011/042720
申请日：2011-06-30
申请人： LIFE TECHNOLOGIES CORPORATION , PETRAUSKENE, Olga , CUMMINGS, Craig , VATTA, Paolo , TEBBS, Robert , BALACHANDRAN, Priya , ZODER, Patrick , WONG, Lily
发明人： PETRAUSKENE, Olga , CUMMINGS, Craig , VATTA, Paolo , TEBBS, Robert , BALACHANDRAN, Priya , ZODER, Patrick , WONG, Lily
IPC分类号： C12Q1/68
CPC分类号： C12Q1/689 , C12Q2600/156 , C12Q2600/16
摘要： Embodiments of the disclosure relate to isolated nucleic acid sequences, methods of use thereof, and workflows for detection of several Listeria species in a sample, particularly in a food or environmental sample. Embodiments of the disclosure may also be used to detect one or more species or strains of Listeria from each other, for example L. grayi may be detected independently of other Listeria spp . Some embodiments also describe a duplexed assay that can detect L. monocytogenes, L. innocua, L. welshimeri, L. seelgeri, L. marthii (formerly incertae-sedis), L. ivanovii, and L. grayi . Kits for detection of Listeria are also described. In some embodiments, methods and kits of the disclosure may comprise a TAQMAN® assay. In some embodiments, 0.2-2 cfu of Listeria spp. are detected using the compositions, methods and kits after a 24-28 hour enrichment period.
摘要翻译：本公开的实施方案涉及分离的核酸序列，其使用方法和用于检测样品中的几种利斯特氏菌物种的工作流程，特别是在食品或环境样品中。本公开的实施方案还可以用于彼此检测李斯特氏菌的一种或多种物种或菌株，例如，可以独立于其他李斯特菌属物种检测L.crilli。一些实施方案还描述了可以检测单核细胞增生李斯特氏菌（L. innocua），威尔逊氏乳杆菌（L.Welshimeri），塞贝尔氏球菌（L.ceelgeri），马氏体（L.marthii）（以前称为incertae-sedis），伊马诺病毒L. 还描述了用于检测李斯特菌的试剂盒。在一些实施方案中，本公开的方法和试剂盒可以包括TAQMAN测定。在一些实施方案中，0.2-2cfu的李斯特菌属在24-28小时浓缩期后使用组合物，方法和试剂盒检测。

6. 发明申请

WO2012018964A1 DETECTION OF SALMONELLA ENTERICA SUBSPECIES ENTERICA SEROVAR ENTERITIDIS IN FOOD AND ENVIRONMENTAL SAMPLES, METHODS AND COMPOSITIONS THEREOF 审中-公开
标题翻译：食品中肠球菌感染的检测食品和环境样品中的肠道输入物，其方法和组合物
公开(公告)号：WO2012018964A1
公开(公告)日：2012-02-09
申请号：PCT/US2011/046512
申请日：2011-08-03
申请人： LIFE TECHNOLOGIES CORPORATION , TEBBS, Robert , CUMMINGS, Craig , NUNEZ, Arlene , BALACHANDRAN, Priya , FATEMI, Payman , PETRAUSKENE, Olga
发明人： TEBBS, Robert , CUMMINGS, Craig , NUNEZ, Arlene , BALACHANDRAN, Priya , FATEMI, Payman , PETRAUSKENE, Olga
IPC分类号： C12Q1/68
CPC分类号： C12Q1/689 , C12Q2561/113 , C12Q2600/156 , Y02A50/451
摘要： Embodiments of the disclosure relate to compositions of isolated nucleic acid sequences, methods, workflows and kits of use thereof for detection of Salmonella enterica , subspecies enterica, serovar Enteritidis ( S . Enteritidis) in a sample, particularly in a food (egg, poultry) or environmental sample (including poultry-related environmental samples). Embodiments of the disclosure may also be used to differentially detect S. Enteritidis without cross-reacting with S. Paratyphi C or other closely related Salmonella species. In some embodiments, methods and kits of the disclosure may comprise a TaqMan® assay. Following sample enrichment, methods of detection may be completed in approximately 3 hours.
摘要翻译：本公开的实施方案涉及分离的核酸序列的组合物，方法，工作流程和其用于检测肠炎沙门氏菌，肠亚科，血清型肠炎沙丁杆菌（肠炎沙丁杆菌）的样品中尤其是食品（鸡蛋，家禽）或环境样品（包括家禽相关环境样品）。本公开的实施方案也可用于差异检测肠杆菌，而不与副伤寒杆菌C或其他密切相关的沙门氏菌属物种交叉反应。在一些实施方案中，本公开的方法和试剂盒可以包含TaqMan测定。样品富集后，检测方法可在约3小时内完成。

7. 发明申请

WO2012145557A1 COMPOSITIONS AND METHODS FOR DETECTING AND IDENTIFYING SALMONELLA ENTERICA STRAINS 审中-公开
标题翻译：用于检测和鉴定沙门氏菌菌丝菌株的组合物和方法
公开(公告)号：WO2012145557A1
公开(公告)日：2012-10-26
申请号：PCT/US2012/034318
申请日：2012-04-19
申请人： LIFE TECHNOLOGIES CORPORATION , CUMMINGS, Craig , BOLCHAKOVA, Elena , FURTADO, Manohar
发明人： CUMMINGS, Craig , BOLCHAKOVA, Elena , FURTADO, Manohar
IPC分类号： C12Q1/68
CPC分类号： C12Q1/689 , C12N15/1089 , C12Q2600/156 , C12Q2600/158 , Y02A50/451
摘要： The present specification describes several novel SNPs of Salmonella enterica subsp. enterica. SNP profiles comprising allelic compositions at each SNP position are described which may be used to identify and differentiate different strains and serovars of Salmonella enterica subsp. enterica. The specification also describes several compositions, methods and kits useful for identifying and differentially distinguishing strains and serovars of Salmonella enterica subsp. enterica.
摘要翻译：本说明书描述了几种新型的肠炎沙门氏菌的SNP。肠炎。描述了包含每个SNP位置的等位基因组成的SNP谱，其可用于鉴定和区分不同的肠炎沙门氏菌菌株和血清型。肠炎。该说明书还描述了用于鉴定和差异区分肠炎沙门氏菌菌株和血清型的几种组合物，方法和试剂盒。肠炎。

8. 发明申请

WO2002066799A1 AIR TURBINE FOR COMBUSTION ENGINE 审中-公开
标题翻译：用于燃烧发动机的空气涡轮机
公开(公告)号：WO2002066799A1
公开(公告)日：2002-08-29
申请号：PCT/US2002/004452
申请日：2002-02-15
申请人： CUMMINGS, Craig , SUTERA, Anthony, J.
发明人： CUMMINGS, Craig , SUTERA, Anthony, J.
IPC分类号： F01N1/02
CPC分类号： F01N13/004 , F01N1/02 , F01N1/06 , F01N1/08 , F01N1/088 , F01N1/12 , F01N1/166 , F01N13/007 , F01N2470/04 , F01N2590/02 , F02D2009/0283 , F02M35/1211 , F02M35/1216 , F02M35/1222 , F02M35/1227 , Y02T10/146
摘要： An air turbine apparatus (10) for use with a combustion engine either as a muffler or as an air intake device is comprised of a housing (12) defining an expansion chamber (20) and an airfoil (50) positioned within the housing (12). The airfoil (50) includes a central aperture (52) extending longitudinally there through that is in fluid communication with both the inlet (18) and the outlet (16) of the housing (12). The airfoil (50) is configured to allow gases flowing through the inlet (18) to flow between the outer edge of the airfoil (50) and the housing (12) and through the aperture (52) of the airfoil (50). In addition, the air travelling through the housing (12) is caused to spin as it travels through the housing (12). This rotational flow increased the rate of flow of gases through the air turbine device (10). An induction tube (22) is provided between the airfoil (50) and the outlet (16) of the housing (12) to encourage turbulent flow of gases without restricting flow to produce a muffling effect and thus a dampening of noise from the combustion engine.
摘要翻译：用作消声器或作为进气装置的内燃机使用的空气涡轮机装置（10）包括限定膨胀室（20）的壳体（12）和定位在壳体（12）内的翼型件）。机翼（50）包括沿其纵向延伸的中心孔（52），其通过与壳体（12）的入口（18）和出口（16）流体连通。机翼（50）构造成允许流过入口（18）的气体在翼型件（50）的外边缘和壳体（12）之间并通过翼型件（50）的孔口（52）流动。此外，通过壳体（12）行进的空气在其穿过壳体（12）时被旋转。该旋转流量增加了气体通过空气涡轮机装置（10）的流量。感应管（22）设置在机翼（50）和壳体（12）的出口（16）之间，以促进气体的湍流而不限制流动，从而产生消声效应，从而减轻来自内燃机。

9. 发明申请

WO2014003997A1 COMPOSITIONS AND METHODS FOR DETECTION OF MULTIPLE MICROORGANISMS 审中-公开
标题翻译：用于检测多种微生物的组合物和方法
公开(公告)号：WO2014003997A1
公开(公告)日：2014-01-03
申请号：PCT/US2013/044180
申请日：2013-06-04
申请人： LIFE TECHNOLOGIES CORPORATION
发明人： TEBBS, Robert , CUMMINGS, Craig , BRZOSKA, Pius , MATHENY, Sharon , O'CONNELL, Catherine , FURTADO, Manohar , SERGEEV, Nikolay , BREVNOV, Maxim
IPC分类号： C12Q1/68
CPC分类号： C12Q1/689 , C12Q2600/16
摘要： The present disclosure describes compositions, methods and kits for detection of one or multiple microorganism contaminants in samples. Some embodiments relate to detecting one or more microorganisms producing virulence factors such as shiga toxin stx1 and stx2 and eae. Some embodiments relate to detection of STEC microorganisms including an E. coli O26, an E. coli O45, an E. coli O103, an E. coli O111, an E. coli O121 or an E. coli O145. In some embodiments, compositions, methods and kits can detect and identify individual serotypes of shiga toxin producing microorganisms. Workflows for multiple microbe detection and identification are also described.
摘要翻译：本公开描述了用于检测样品中一种或多种微生物污染物的组合物，方法和试剂盒。一些实施方案涉及检测产生毒力因子的一种或多种微生物，例如志贺毒素stx1和stx2和eae。一些实施方案涉及检测STEC微生物，包括大肠杆菌O26，大肠杆菌O45，大肠杆菌O103，大肠杆菌O111，大肠杆菌O121或大肠杆菌O145。在一些实施方案中，组合物，方法和试剂盒可以检测和鉴定产生志贺毒素的微生物的个体血清型。还描述了用于多个微生物检测和鉴定的工作流程。

10. 发明申请

WO2013151647A1 COMPOSITIONS AND METHODS FOR DETECTION OF MYCOBACTERIUM AVIUM PARATUBERCULOSIS 审中-公开
标题翻译：用于检测艾滋病病原菌的组合物和方法
公开(公告)号：WO2013151647A1
公开(公告)日：2013-10-10
申请号：PCT/US2013/029072
申请日：2013-03-05
申请人： LIFE TECHNOLOGIES CORPORATION
发明人： JI, Yongmei , BRZOSKA, Pius , BURRELL, Angela , CUMMINGS, Craig , O´CONNELL, Caytherine
IPC分类号： C12Q1/68
CPC分类号： C12Q1/689 , C12Q1/6806 , C12Q2600/112 , C12Q2600/16
摘要： Disclosed are compositions, assays, methods, diagnostic methods, kits and diagnostic kits for the specific and differential detection of Mycobacterium avium subsp. paratuberculosis from samples including veterinary samples, clinical samples, food samples, forensic sample, an environmental sample (e.g., soil, dirt, garbage, sewage, air, or water), including food processing and manufacturing surfaces, or a biological sample.
摘要翻译：公开了用于特异性和差异性检测鸟分枝杆菌亚种的组合物，测定法，方法，诊断方法，试剂盒和诊断试剂盒。来自包括兽医样品，临床样品，食品样品，法医样品，环境样品（例如土壤，污垢，垃圾，污水，空气或水）的样品中的副结核病，包括食品加工和制造表面或生物样品。

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