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1. 发明申请

WO2012003412A3 INDUCIBLE NUCLEIC ACID TARGETS FOR DETECTION OF PATHOGENS, METHODS AND COMPOSITIONS THEREOF 审中-公开
公开(公告)号：WO2012003412A3
公开(公告)日：2012-01-05
申请号：PCT/US2011/042723
申请日：2011-06-30
申请人： LIFE TECHNOLOGIES CORPORATION , BALACHANDRAN, Priya , CUMMINGS, Craig , PETRAUSKENE, Olga , PAWAR, Hemant , TEBBS, Robert , FURTADO, Manohar
发明人： BALACHANDRAN, Priya , CUMMINGS, Craig , PETRAUSKENE, Olga , PAWAR, Hemant , TEBBS, Robert , FURTADO, Manohar
IPC分类号： C12Q1/68
摘要： The present application describes compositions, methods and kits for rapid detection and identification of various microorganisms using inducible RNA. Methods for rapidly detecting microorganisms by detecting expression of inducible RNA of target genes following induction of a target gene using an inducer are described. Some embodiments describe methods and workflows for rapidly detecting microbes such as, but not limited to, Salmonella spp, Listeria spp. and Vibrio spp. Compositions and kits comprise primer nucleic acid sequences having hybridization specificity for priming amplification of genes of microorganisms (or gene fragments) that are responsive to one or more RNA-inducing agents. In some embodiments, kits and compositions further comprise probe nucleic acid sequences having hybridization specificity for genes responsive to RNA-inducing agents or fragments thereof.

2. 发明申请

WO2013029021A1 COMPOSITIONS AND METHODS FOR DETECTION OF MULTIPLE MICROORGANISMS 审中-公开
标题翻译：用于检测多种微生物的组合物和方法
公开(公告)号：WO2013029021A1
公开(公告)日：2013-02-28
申请号：PCT/US2012/052383
申请日：2012-08-24
申请人： LIFE TECHNOLOGIES CORPORATION , TEBBS, Robert , CUMMINGS, Craig , BRZOSKA, Pius , MATHENY, Sharon , O'CONNELL, Catherine , FURTADO, Manohar , FANG, Rixun , PETRAUSKENE, Olga , LIEW, Sueh-Ning , SCHUMAKER, Michael
发明人： TEBBS, Robert , CUMMINGS, Craig , BRZOSKA, Pius , MATHENY, Sharon , O'CONNELL, Catherine , FURTADO, Manohar , FANG, Rixun , PETRAUSKENE, Olga , LIEW, Sueh-Ning , SCHUMAKER, Michael
IPC分类号： C12Q1/68
CPC分类号： C12Q1/686 , C12Q1/689 , C12Q2600/16
摘要： The present teachings describe compositions, methods and kits for detection of one or multiple microorganism contaminants in samples. Some embodiments relate to detecting one or more microorganisms producing virulence factors such as a shiga toxin ( stx ) or an eae . In some embodiments, compositions, methods and kits can detect and identify individual strains and serotypes of shiga toxin producing microorganisms. Some embodiments describe compositions, methods and kits for detecting STEC microbes. Workflows for multiple microbe detection and identification are also described.
摘要翻译：本教导描述了用于检测样品中一种或多种微生物污染物的组合物，方法和试剂盒。一些实施方案涉及检测产生毒力因子（例如志贺毒素（stx）或eae）的一种或多种微生物。在一些实施方案中，组合物，方法和试剂盒可以检测和鉴定产生志贺毒素的微生物的个体菌株和血清型。一些实施方案描述了用于检测STEC微生物的组合物，方法和试剂盒。还描述了用于多个微生物检测和鉴定的工作流程。

3. 发明申请

WO2011053871A2 MULTI-PRIMER ASSAY FOR MYCOPLASMA DETECTION 审中-公开
标题翻译：多发性骨髓瘤检测
公开(公告)号：WO2011053871A2
公开(公告)日：2011-05-05
申请号：PCT/US2010/054867
申请日：2010-10-29
申请人： LIFE TECHNOLOGIES CORPORATION , PETRAUSKENE, Olga , BRZOSKA, Pius , BIT, Somaya , LIU, Jen-Kuei , TEBBS, Robert , FURTADO, Manohar
发明人： PETRAUSKENE, Olga , BRZOSKA, Pius , BIT, Somaya , LIU, Jen-Kuei , TEBBS, Robert , FURTADO, Manohar
IPC分类号： C12Q1/68 , C07H21/04
CPC分类号： C12Q1/689 , C12Q2600/16
摘要： Disclosed is a multi-primer amplification assay, method and kits for detecting Mycoplasma species and closely related species utilizing a plurality of oligonucleotide primers in contact with a sample in a single vessel and detecting the amplification product, wherein the presence of an amplification product indicates Mycoplasma in the sample.
摘要翻译：公开了利用与单个容器中的样品接触的多个寡核苷酸引物并检测扩增产物的用于检测支原体物种和紧密相关物种的多引物扩增测定法，方法和试剂盒，其中扩增产物的存在表明样品中的支原体。

4. 发明申请

WO2010111509A1 DISCRIMINATORY POSITIVE/EXTRACTION CONTROL DNA 审中-公开
标题翻译：歧视积极/提取控制DNA
公开(公告)号：WO2010111509A1
公开(公告)日：2010-09-30
申请号：PCT/US2010/028693
申请日：2010-03-25
申请人： LIFE TECHNOLOGIES CORPORATION , BREWER, Michael , PETRAUSKENE, Olga , LIU, Jen-Kuei , CUMMINGS, Craig , LIEW, Sueh-Ning , FURTADO, Manohar
发明人： BREWER, Michael , PETRAUSKENE, Olga , LIU, Jen-Kuei , CUMMINGS, Craig , LIEW, Sueh-Ning , FURTADO, Manohar
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6851 , C12Q2561/107 , C12Q2545/107 , C12Q2537/107
摘要： The present teachings generally relate to methods and kits incorporating a discriminating positive control for determining whether a particular microorganism or group of microorganisms are present in a sample, for example but not limited to a food, environmental, agricultural, biopharmaceutical, pharmaceutical, or water sample. According to certain methods, at least part of a starting material, for example but not limited to, a food, environmental, agricultural, biopharmaceutical, pharmaceutical, or water sample can be combined with a culture medium and incubated under conditions suitable for microbial growth followed by extracting microorganism and added control nucleic acids for analysis. The extracted nucleic acids are amplified and the amplified nucleic acids are detected, directly or indirectly, and the fidelity of the methods and the presence or absence of the corresponding microorganism is determined because the discriminating positive control provides both confirmatory results for the methods used but eliminates false positive results.
摘要翻译：本教导通常涉及包含用于确定特定微生物或一组微生物是否存在于样品中的鉴别性阳性对照的方法和试剂盒，例如但不限于食品，环境，农业，生物制药，药物或水样品。根据某些方法，至少部分原料，例如但不限于食品，环境，农业，生物制药，药物或水样品可以与培养基组合，并在适合于微生物生长的条件下温育通过提取微生物和添加的对照核酸进行分析。所提取的核酸被扩增并且直接或间接检测扩增的核酸，并且确定方法的保真度和相应的微生物的存在或不存在，因为鉴别性阳性对照提供所使用的方法的确认结果但消除假阳性结果。

5. 发明申请

WO2010132832A1 E. COLI O157:H7 SPECIFIC ASSAY 审中-公开
标题翻译：大肠杆菌O157：H7特异性测定
公开(公告)号：WO2010132832A1
公开(公告)日：2010-11-18
申请号：PCT/US2010/034998
申请日：2010-05-14
申请人： LIFE TECHNOLOGIES CORPORATION , VATTA, Paolo , PETRAUSKENE, Olga , FURTADO, Manohar , BRZOSKA, Pius , WONG, Lily , BARKER, Melissa , CUMMINGS, Craig, A.
发明人： VATTA, Paolo , PETRAUSKENE, Olga , FURTADO, Manohar , BRZOSKA, Pius , WONG, Lily , BARKER, Melissa , CUMMINGS, Craig, A.
IPC分类号： C12Q1/68
CPC分类号： C12Q1/689 , C12Q2600/142
摘要： Disclosed are assays, methods and kits for the specific detection of E. coli 0157:H7 and not E. coli O55:H7 from complex food matrices, water, a beverage sample, a fermentation broth, a forensic sample, an environmental sample ( e.g. , soil, dirt, garbage, sewage, air, or water), including food processing and manufacturing surfaces, or a biological sample.
摘要翻译：公开了用于特异性检测大肠杆菌0157：H7而不是大肠杆菌O55：H7的测定，方法和试剂盒，来自复合食物基质，水，饮料样品，发酵液，法医样品，环境样品（例如，土壤，污垢，垃圾，污水，空气或水），包括食品加工和制造表面，或生物样品。

6. 发明申请

WO2009143499A1 METHODS AND KITS FOR EXTRACTION OF DNA 审中-公开
标题翻译：用于提取DNA的方法和试剂盒
公开(公告)号：WO2009143499A1
公开(公告)日：2009-11-26
申请号：PCT/US2009/045092
申请日：2009-05-22
申请人： LIFE TECHNOLOGIES CORPORATION , BREVNOV, Maxim, G. , PAWAR, Hemant, S. , FURTADO, Manohar , SHEWALE, Jaiprakash, G.
发明人： BREVNOV, Maxim, G. , PAWAR, Hemant, S. , FURTADO, Manohar , SHEWALE, Jaiprakash, G.
IPC分类号： C12N15/10
CPC分类号： C12N15/1013
摘要： Methods and materials are disclosed for use in recovering a biopolymer from a solution. In particular, the invention provides methods for extraction and isolation of nucleic acids from biological materials. The nucleic acids can be separated by forming a stable complex with soluble polysaccharide polymers and magnetic particles, in the presence of detergents and solvent. When the particles are magnetically separated out of the solution, the nucleic acids are separated with them. The nucleic acids can subsequently be released and separated from the particles. The nucleic acid preparation is useful for achieving efficient and accurate results in downstream molecular techniques such as quantification, identification of the source of the nucleic acids, and genotyping.
摘要翻译：公开了用于从溶液中回收生物聚合物的方法和材料。特别地，本发明提供了用于从生物材料中提取和分离核酸的方法。核酸可以通过在洗涤剂和溶剂存在下与可溶性多糖聚合物和磁性颗粒形成稳定的复合物来分离。当颗粒从溶液中磁性分离出来时，核酸与它们分离。核酸随后可以被释放并与颗粒分离。核酸制备可用于在下游分子技术中实现有效且准确的结果，例如定量，核酸来源的鉴定和基因分型。

7. 发明申请

WO2012145557A1 COMPOSITIONS AND METHODS FOR DETECTING AND IDENTIFYING SALMONELLA ENTERICA STRAINS 审中-公开
标题翻译：用于检测和鉴定沙门氏菌菌丝菌株的组合物和方法
公开(公告)号：WO2012145557A1
公开(公告)日：2012-10-26
申请号：PCT/US2012/034318
申请日：2012-04-19
申请人： LIFE TECHNOLOGIES CORPORATION , CUMMINGS, Craig , BOLCHAKOVA, Elena , FURTADO, Manohar
发明人： CUMMINGS, Craig , BOLCHAKOVA, Elena , FURTADO, Manohar
IPC分类号： C12Q1/68
CPC分类号： C12Q1/689 , C12N15/1089 , C12Q2600/156 , C12Q2600/158 , Y02A50/451
摘要： The present specification describes several novel SNPs of Salmonella enterica subsp. enterica. SNP profiles comprising allelic compositions at each SNP position are described which may be used to identify and differentiate different strains and serovars of Salmonella enterica subsp. enterica. The specification also describes several compositions, methods and kits useful for identifying and differentially distinguishing strains and serovars of Salmonella enterica subsp. enterica.
摘要翻译：本说明书描述了几种新型的肠炎沙门氏菌的SNP。肠炎。描述了包含每个SNP位置的等位基因组成的SNP谱，其可用于鉴定和区分不同的肠炎沙门氏菌菌株和血清型。肠炎。该说明书还描述了用于鉴定和差异区分肠炎沙门氏菌菌株和血清型的几种组合物，方法和试剂盒。肠炎。

8. 发明申请

WO2012174119A2 COMPOSITIONS AND METHODS FOR DETECTION OF CRONOBACTER SPP. AND CRONOBACTER SPECIES AND STRAINS 审中-公开
标题翻译：用于检测CRONOBACTER SPP的组合物和方法。和克隆物种和菌株
公开(公告)号：WO2012174119A2
公开(公告)日：2012-12-20
申请号：PCT/US2012/042263
申请日：2012-06-13
申请人： LIFE TECHNOLOGIES CORPORATION , JI, Yongmei , SHIH, Sheung-Mei ("Rita") , DEGORICIJA, Lovorka , ZHOU, Zhaohui , CUMMINGS, Craig , FURTADO, Manohar , BRZOSKA, Pius , BURRELL, Angela , LEONG, Harrison , FANG, Xingwang , BOUNPHENG, Mangkey , SHAH, Rohan
发明人： JI, Yongmei , SHIH, Sheung-Mei ("Rita") , DEGORICIJA, Lovorka , ZHOU, Zhaohui , CUMMINGS, Craig , FURTADO, Manohar , BRZOSKA, Pius , BURRELL, Angela , LEONG, Harrison , FANG, Xingwang , BOUNPHENG, Mangkey , SHAH, Rohan
IPC分类号： C12Q1/68
CPC分类号： C12Q1/689 , C12R1/01
摘要： Disclosed are genomic sequences for nine strains of C ronobacter spp . (C. sakazakii - 696, 701, 680; C. malonaticus - 507, 681; C. turicensis - 564; C. muytjensii - 530; C. dublinensis - 582; C. genomospl - 581) and compositions, methods, and kits for detecting, identifying and distinguishing C ronobacter spp . strains from each other and from non-C ronobacter spp . strains. Some embodiments describe isolated nucleic acid compositions unique to certain C ronobacter strains as well as compositions that are specific to all C ronobacter spp . Primer and probe compositions and methods of use of primers and probes are also provided. Kits for identification of C ronobacter spp . are also described. Some embodiments relate to computer software methods for setting a control based threshold for analysis of PCR
摘要翻译：公开了九个克罗杆菌属菌株的基因组序列。（C. sakazakii-696,701,680; C. malonaticus-507,681; C. turicensis-564; C. muytjensii-530; C.dublinensis-582; C.基因组蛋白-58）和组合物，方法和试剂盒用于检测，识别和鉴别克罗杆菌属。菌株彼此和非克罗杆菌属。株。一些实施方案描述了某些克罗杆菌菌株特有的分离的核酸组合物以及对所有克罗杆菌属特异性的组合物。还提供引物和探针组合物以及引物和探针的使用方法。克隆杆菌属鉴定试剂盒也被描述。一些实施例涉及用于设置用于PCR分析的基于控制的阈值的计算机软件方法

9. 发明申请

WO2012006116A2 METHODS, WORKFLOWS, KITS, APPARATUSES, AND COMPUTER PROGRAM MEDIA FOR NUCLEIC ACID SAMPLE PREPARATION FOR NUCLEIC ACID SEQUENCING 审中-公开
标题翻译：用于核酸序列的核酸样品制备的方法，工作流程，工具，设备和计算机程序介质
公开(公告)号：WO2012006116A2
公开(公告)日：2012-01-12
申请号：PCT/US2011/042240
申请日：2011-06-28
申请人： LIFE TECHNOLOGIES CORPORATION , BREVNOV, Maxim , BOLCHAKOVA, Elena , FURTADO, Manohar
发明人： BREVNOV, Maxim , BOLCHAKOVA, Elena , FURTADO, Manohar
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6806 , C12Q1/6869 , C12Q2563/131 , C12Q2563/143
摘要： A method for preparing a nucleic acid sample for nucleic acid sequencing includes amplifying a nucleic acid target sequence using a primer bound to a first capture substrate; capturing an amplified nucleic acid product by the first capture substrate; generating at least one sequencing ladder from the amplified nucleic acid product using at least one sequencing primer; capturing the at least one sequencing ladder by hybridizing the at least one sequencing ladder to a complementary capture compound on a second capture substrate; and removing the at least one sequencing ladder from the second capture substrate. The first and/or second capture substrate may include a magnetic particle. Other methods, workflows, kits, and computer program media for nucleic acid sample preparation are also disclosed.
摘要翻译：用于制备用于核酸测序的核酸样品的方法包括使用与第一捕获底物结合的引物扩增核酸靶序列; 通过第一捕获基质捕获扩增的核酸产物; 使用至少一个测序引物从扩增的核酸产物产生至少一个测序梯; 通过将所述至少一个测序梯度杂交到第二捕获基底上的互补捕获化合物来捕获所述至少一个测序梯; 以及从所述第二捕获基板去除所述至少一个测序梯。第一和/或第二捕获基板可以包括磁性颗粒。还公开了用于核酸样品制备的其它方法，工作流程，试剂盒和计算机程序培养基。

10. 发明申请

WO2011032054A3 ANALYSIS OF Y-CHROMOSOME STR MARKERS 审中-公开
标题翻译： Y染色体STR标记的分析
公开(公告)号：WO2011032054A3
公开(公告)日：2011-03-17
申请号：PCT/US2010/048542
申请日：2010-09-10
申请人： LIFE TECHNOLOGIES CORPORATION , FANG, Rixun , FURTADO, Manohar , KAYSER, Manfred
发明人： FANG, Rixun , FURTADO, Manohar , KAYSER, Manfred
IPC分类号： C12Q1/68 , C12N15/12 , C40B40/08
摘要： The methods and compositions provided herein relate to the discovery of 13 STR are markers found on the human Y chromosome with surprisingly high mutation rates when compared with 173 other Y-STR markers known today, including those that are in common use in forensics. In addition to theoretical expectations based on elevated mutation rates, these 13 rapidly-mutating (RM) Y-STRs proved to be suitable for differentiating between closely related males, as well as between more distant male relatives and much more so than the most-commonly used Y-STRs in forensics. Our new set of RM-Y-STRs is expected to overcome the current dilemma of Y-chromosome analysis in forensic applications from current male lineage identification towards male individual identification in many cases, due to their extraordinary mutation properties as discovered here. Embodiments of the invention include methods for allelic determination of rapidly-mutating Y-STR markers, amplification primers for the analysis of rapidly-mutating Y-STR markers, allelic ladders for analysis of rapidly-mutating Y-STR markers, and kits for the analysis of rapidly-mutating Y-STR markers.
摘要翻译：本文提供的方法和组合物涉及发现13个STR是在人类Y染色体上发现的标记，与当前已知的173种其他Y-STR标记相比，具有令人惊讶的高突变率，包括那些在法医学中常用。除了基于提高的突变率的理论预期之外，这13个快速突变（RM）Y-STR被证明适用于区分密切相关的雄性以及更远距离的男性亲属之间，以及最常见的在取证中使用了Y-STR。由于在这里发现的非凡突变特性，我们的新一套RM-Y-STRs预计将克服当前男性谱系鉴定到男性个体鉴定的法医应用中Y染色体分析的困境。本发明的实施方案包括用于快速突变的Y-STR标志物的等位基因测定的方法，用于快速突变的Y-STR标志物的分析的扩增引物，用于分析快速突变的Y-STR标志物的等位基因梯级以及用于分析的试剂盒的快速突变的Y-STR标记。

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