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1. 发明申请

WO2009086218A2 DETECTION OF ANTIBIOTIC-RESISTANT MICROORGANISMS 审中-公开
标题翻译：检测抗生素微生物
公开(公告)号：WO2009086218A2
公开(公告)日：2009-07-09
申请号：PCT/US2008/087859
申请日：2008-12-19
申请人： GEN-PROBE INCORPORATED , BECKER, Michael, M. , GAO, Kui , LAM, Wai-Chung
发明人： BECKER, Michael, M. , GAO, Kui , LAM, Wai-Chung
IPC分类号： C12Q1/68
CPC分类号： C12Q1/689 , C12Q2600/16 , C12Q2545/114 , C12Q2545/101 , C12Q2531/113
摘要： Method of detecting methicillin-resistant S. aureus (MRSA) and methicillin-sensitive S. aureus (MSSA) in a nucleic acid coamplification assay. The invention advantageously reduces the incidence of false-positive MRSA determinations in real-time assays by requiring satisfaction of a threshold criterion that excludes certain co-infections from the MRSA determination. The invention further provides for determination of MSSA, even when the MSSA is present in combination with methicillin-resistant coagulase-negative (MR-CoNS) bacteria at high or low levels.
摘要翻译：在核酸共扩增测定中检测耐甲氧西林的金黄色葡萄球菌（MRSA）和甲氧西林敏感的金黄色葡萄球菌（MSSA）的方法。本发明通过要求满足从MRSA测定中排除某些共同感染的阈值标准而有利地降低了实时测定中假阳性MRSA测定的发生率。即使当MSSA以高或低水平与耐甲氧西林凝固酶阴性（MR-CoNS）细菌联合存在时，本发明还提供了MSSA的测定。

2. 发明申请

WO2006026388A2 SINGLE-PRIMER NUCLEIC ACID AMPLIFICATION METHODS 审中-公开
标题翻译：单一核酸核酸扩增方法
公开(公告)号：WO2006026388A2
公开(公告)日：2006-03-09
申请号：PCT/US2005/030329
申请日：2005-08-26
申请人： GEN-PROBE INCORPORATED , BECKER, Michael, M. , LAM, Wai-Chung , LIVEZEY, Kristin, W. , BRENTANO, Steven, T. , KOLK, Daniel, P. , SCHRODER, Astrid, R.W.
发明人： BECKER, Michael, M. , LAM, Wai-Chung , LIVEZEY, Kristin, W. , BRENTANO, Steven, T. , KOLK, Daniel, P. , SCHRODER, Astrid, R.W.
IPC分类号： C12Q1/68 , C12P19/34
CPC分类号： C12P19/34 , C12Q1/6865 , C12Q2537/163 , C12Q2521/325 , C12Q2521/107 , C12Q2525/186 , C12Q2533/101 , C12Q2525/143
摘要： The present invention is directed to novel methods of synthesizing multiple copies of a target nucleic acid sequence which are autocatalytic ( i.e ., able to cycle automatically without the need to modify reaction conditions such as temperature, pH, or ionic strength and using the product of one cycle in the next one). In particular, the present invention discloses a method of nucleic acid amplification which is robust and efficient, while reducing the appearance of side-products. The method uses only one primer, the "priming oligonucleotide," a 3'blocked promoter oligonucleotide and optionally, a means for terminating a primer extension reaction, to amplify RNA or DNA molecules in vitro , while reducing or eliminating the formation of side-products. The method of the present invention minimizes or eliminates the emergence of side-products, thus providing a high level of specificity. Furthermore, the appearance of side-products can complicate the analysis of the amplification reaction by various molecular detection techniques. The present invention minimizes or eliminates this problem, thus providing an enhanced level of sensitivity.
摘要翻译：本发明涉及合成多个拷贝的目标核酸序列的新方法，所述靶核酸序列是自动催化的（<

3. 发明申请

WO2007146154A1 TAGGED OLIGONUCLEOTIDES AND THEIR USE IN NUCLEIC ACID AMPLIFICATION METHODS 审中-公开
标题翻译：标记的寡核苷酸及其在核酸扩增方法中的应用
公开(公告)号：WO2007146154A1
公开(公告)日：2007-12-21
申请号：PCT/US2007/013553
申请日：2007-06-06
申请人： GEN-PROBE INCORPORATED , BECKER, Michael, M. , LIVEZEY, Kristin, W. , LAM, Wai-Chung
发明人： BECKER, Michael, M. , LIVEZEY, Kristin, W. , LAM, Wai-Chung
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6853 , C12Q1/6848 , C12Q1/6865 , C12Q2525/301 , C12Q2525/186 , C12Q2525/155 , C12Q2525/161
摘要： The present invention provides nucleic acid amplification methods that desirably reduce or eliminate false positive amplification signals resulting from contaminating biological material, e.g., nucleic acid, that may be present in one or more reagents used in an amplification reaction and/or that may be present in the environment in which an amplification reaction is performed. The invention offers the further advantage of requiring less stringent purification and/or sterility efforts than conventionally needed in order to ensure that enzymes and other reagents used in amplification reactions, and the environment in which an amplification reaction is performed, are free of bacterial or other nucleic acid contamination that may yield false positive results.
摘要翻译：本发明提供了核酸扩增方法，其期望地减少或消除由可能存在于扩增反应中使用的一种或多种试剂中的生物材料例如核酸引起的假阳性扩增信号和/或可存在于进行扩增反应的环境。本发明提供了进一步的优点，即需要比常规所需的更少的纯化和/或不育努力，以确保用于扩增反应的酶和其它试剂以及进行扩增反应的环境中没有细菌或其它可能产生假阳性结果的核酸污染。

4. 发明申请

WO2008108843A2 METHODS AND KITS FOR AMPLIFYING DNA 审中-公开
标题翻译：扩增DNA的方法和试剂盒
公开(公告)号：WO2008108843A2
公开(公告)日：2008-09-12
申请号：PCT/US2007/063103
申请日：2007-03-01
申请人： GEN-PROBE INCORPORATED , BECKER, Michael, M. , LAM, Wai-Chung , LIVEZEY, Kristin, W. , BRENTANO, Steven, T. , KOLK, Daniel, P. , SCHRODER, Astrid, R.W.
发明人： BECKER, Michael, M. , LAM, Wai-Chung , LIVEZEY, Kristin, W. , BRENTANO, Steven, T. , KOLK, Daniel, P. , SCHRODER, Astrid, R.W.
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6865 , C12Q2533/101 , C12Q2525/186 , C12Q2521/107
摘要： Novel methods of synthesizing multiple copies of a target nucleic acid sequence which are autocatalytic are disclosed (/. e., able to cycle automatically without the need to modify reaction conditions such as temperature, pH, or ionic strength and using the product of one cycle in the next one). In particular, methods of nucleic acid amplification are disclosed which are robust and efficient, while reducing the appearance of side-products. In general, the methods use priming oligonucleotides that target only one sense of a target nucleic acid, a promoter oligonucleotide modified to prevent polymerase extension from its 3 '-terminus and, optionally, a means for terminating a primer extension reaction, to amplify RNA or DNA molecules in vitro, while reducing or substantially eliminating the formation of side-products. The disclosed methods minimizes or substantially eliminate the emergence of side-products, thus providing ahigh level of specificity. Furthermore, the appearance of side-products can complicate the analysis of the amplification reaction by various molecular detection techniques. The disclosed methods minimize or substantially eliminate this problem, thus providing enhanced levels of sensitivity.
摘要翻译：公开了合成自催化的靶核酸序列的多个拷贝的新方法（即，能够自动循环而不需要修改诸如温度，pH或离子的反应条件力量和使用下一个周期的产品）。具体而言，公开了稳定且有效的核酸扩增方法，同时减少副产物的出现。一般而言，所述方法使用仅靶向靶核酸的一种意义的引发寡核苷酸，经修饰以阻止聚合酶从其3'-末端延伸的启动子寡核苷酸，以及任选地终止引物延伸反应的手段，以扩增RNA或体外DNA分子，同时减少或基本消除副产物的形成。所公开的方法最小化或基本上消除了副产物的出现，从而提供了高水平的特异性。此外，副产物的出现可能会使各种分子检测技术对扩增反应的分析复杂化。所公开的方法最小化或基本上消除了这个问题，因此提供了增强的灵敏度水平。

5. 发明申请

WO2010151566A1 COMPOSITIONS AND METHODS FOR DETECTING NUCLEIC ACID FROM MOLLICUTES 审中-公开
标题翻译：用于检测来自麦芽糖的核酸的组合物和方法
公开(公告)号：WO2010151566A1
公开(公告)日：2010-12-29
申请号：PCT/US2010/039592
申请日：2010-06-23
申请人： GEN-PROBE INCORPORATED , KAPLAN, Shannon, K. , LIVEZEY, Kristin, W. , BECKER, Michael, M. , HOGAN, James, D.
发明人： KAPLAN, Shannon, K. , LIVEZEY, Kristin, W. , BECKER, Michael, M. , HOGAN, James, D.
IPC分类号： C12Q1/68
CPC分类号： C12Q1/689 , C12Q2600/16
摘要： Compositions, reaction mixtures, kits and methods used in amplifying and detecting nucleic acids from various species of the class Mollicutes. In certain aspects and embodiments, particular regions of the 23S rRNA or gene encoding said rRNA have been identified as preferred targets for nucleic acid amplification reactions of a sample suspected containing at least one species of Mollicutes. Some oligomers comprise tag regions. Some oligomers comprise target closing regions, promoter sequences, and/or binding moieties, such as homopolymeric and heteropolymeric nucleotide sequences. Samples can be from any source suspected of containing a species of the class Mollicutes. Preferred sample sources include bioreactors, cell lines, cell culture wares and pharmaceutical manufacturing wares, to name a few.
摘要翻译：用于扩增和检测来自Mollicutes类的各种物种的核酸的组合物，反应混合物，试剂盒和方法。在某些方面和实施方案中，编码所述rRNA的23S rRNA或基因的特定区域已被鉴定为怀疑含有至少一种Mollicutes的样品的核酸扩增反应的优选靶标。一些低聚物包括标签区。一些寡聚体包含靶闭合区，启动子序列和/或结合部分，例如均聚和杂聚核苷酸序列。样品可以来自怀疑含有Mollicutes类物种的任何来源。优选的样品来源包括生物反应器，细胞系，细胞培养物和药物制造商品等等。

6. 发明申请

WO2010126913A1 METHODS AND KITS FOR USE IN THE SELECTIVE AMPLIFICATION OF TARGET SEQUENCES 审中-公开
标题翻译：用于选择性扩增目标序列的方法和工具
公开(公告)号：WO2010126913A1
公开(公告)日：2010-11-04
申请号：PCT/US2010/032631
申请日：2010-04-27
申请人： GEN-PROBE INCORPORATED , BECKER, Michael, M. , LIVEZEY, Kristin, W.
发明人： BECKER, Michael, M. , LIVEZEY, Kristin, W.
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6848 , C12Q1/6865 , C12Q2531/143 , C12Q2525/301
摘要： The present invention provides nucleic acid amplification methods that desirably reduce or eliminate false positive amplification signals resulting from contaminating biological material, e.g., nucleic acid, that may be present in one or more reagents used in an amplification reaction and/or that may be present in the environment in which an amplification reaction is performed. The invention offers the further advantage of requiring less stringent purification and/or sterility efforts than conventionally needed in order to ensure that enzymes and other reagents used in amplification reactions, and the environment in which an amplification reaction is performed, are free of bacterial or other nucleic acid contamination that may yield false positive results.
摘要翻译：本发明提供了核酸扩增方法，其期望地减少或消除由污染生物材料（例如核酸）产生的假阳性扩增信号，所述核酸可以存在于用于扩增反应的一种或多种试剂中和/或可存在于进行扩增反应的环境。本发明提供了进一步的优点，其需要比常规所需的更少的纯化和/或不育努力，以确保用于扩增反应的酶和其它试剂以及进行扩增反应的环境不含细菌或其它可能产生假阳性结果的核酸污染物。

7. 发明申请

WO2009140374A2 INACTIVATABLE TARGET CAPTURE OLIGOMERS FOR USE IN THE SELECTIVE HYBRIDIZATION AND CAPTURE OF TARGET NUCLEIC ACID SEQUENCES 审中-公开
标题翻译：用于选择性杂交和捕获目标核酸序列的无效目标捕获寡聚体
公开(公告)号：WO2009140374A2
公开(公告)日：2009-11-19
申请号：PCT/US2009/043775
申请日：2009-05-13
申请人： GEN-PROBE INCORPORATED , BECKER, Michael, M. , LIVEZEY, Kristin, W.
发明人： BECKER, Michael, M. , LIVEZEY, Kristin, W.
IPC分类号： C12Q1/68 , C07H21/00 , G01N33/566
CPC分类号： C12Q1/6816 , C12Q1/6832 , C12Q1/6834 , C12Q1/686 , C12Q2525/161 , C12Q2525/301 , C12Q2565/107 , C12Q2563/179 , C12Q2527/101 , C12Q2537/1373
摘要： The present invention provides compositions, kits and methods for the selective hybridization and capture of a specific target nucleic acid. The specific target nucleic acid may be present in a heterogeneous mixture of nucleic acids. Selective hybridization and capture provides a target nucleic acid that is substantially free of non-target and/or contaminating nucleic acids. Target nucleic acids that have been selectively hybridized and captured using the current invention are then used in subsequent analysis, wherein the presence of non-target and/or contaminating nucleic acids that interfere with said subsequent analysis have been substantially reduced or eliminated, thereby providing improved analysis results. The invention offers the further advantage of requiring less stringent purification and/or sterility efforts than conventionally needed in order to ensure that enzymes and other reagents used in subsequent analysis, or present in the environment in which an assay is performed, are free of bacterial or other contaminating nucleic acids.
摘要翻译：本发明提供用于选择性杂交和捕获特异性靶核酸的组合物，试剂盒和方法。特异性靶核酸可以存在于核酸的非均相混合物中。选择性杂交和捕获提供基本上不含非靶和/或污染性核酸的靶核酸。然后使用本发明选择性杂交和捕获的目标核酸用于随后的分析中，其中干扰所述后续分析的非目标和/或污染性核酸的存在已被大大减少或消除，从而提供改进的分析结果。本发明提供了进一步的优点，其需要比常规所需的更少的纯化和/或不育努力，以确保用于后续分析中或存在于进行测定的环境中的酶和其它试剂不含细菌或其他污染性核酸。

8. 发明申请

WO2007030505A1 METHODS, COMPOSITIONS AND KITS FOR ISOTHERMAL AMPLIFICATION OF NUCLEIC ACIDS 审中-公开
标题翻译：方法，用于核酸的同位素放大的组合物和试剂盒
公开(公告)号：WO2007030505A1
公开(公告)日：2007-03-15
申请号：PCT/US2006/034663
申请日：2006-09-06
申请人： GEN-PROBE INCORPORATED , BECKER, Michael, M. , LIVEZEY, Kristin, W.
发明人： BECKER, Michael, M. , LIVEZEY, Kristin, W.
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6844 , C12P19/34 , C12Q1/6853 , C12Q2527/101 , C12Q2537/155 , C12Q2525/161 , C12Q2525/179 , C12Q2537/1376
摘要： Methods and compositions are described for isothermal nucleic acid amplification of a nucleic acid template strand by using an oligonucleotide primer that includes an AT-rich nucleotide sequence and a polymerase having strand displacement activity.
摘要翻译：描述了通过使用包含富含AT的核苷酸序列的寡核苷酸引物和具有链置换活性的聚合酶的核酸模板链的等温核酸扩增的方法和组合物。

9. 发明申请

WO2012037531A1 CAPTURE PROBES IMMOBILIZABLE VIA L-NUCLEOTIDE TAIL 审中-公开
标题翻译：捕获探针通过L-核核酸尾部可维持
公开(公告)号：WO2012037531A1
公开(公告)日：2012-03-22
申请号：PCT/US2011/052050
申请日：2011-09-16
申请人： Gen-Probe Incorporated , POLLNER, Reinhold , MAJLESSI, Mehrdad , YAMAGATA, Susan , BECKER, Michael, M. , REYNOLDS, Mark , ARNOLD, Lyle
发明人： POLLNER, Reinhold , MAJLESSI, Mehrdad , YAMAGATA, Susan , BECKER, Michael, M. , REYNOLDS, Mark , ARNOLD, Lyle
IPC分类号： C12Q1/68
CPC分类号： C12Q1/707 , C12Q1/6837 , C12Q1/703 , Y02A50/53 , Y02A50/54 , C12Q2525/161
摘要： The invention provides chimeric capture probes immobilizable via an L-nucleic acid tail that can bind to a complementary L-nucleic acid in an immobilized probe. The capture probes are useful for capturing a target nucleic acid from a sample. The L-nucleic acid in the tail of the capture probe bind to the complementary L-nucleic acid in the immobilized probe with similar affinity as would otherwise equivalent D-nucleic acids. However, the L-nucleic acid of the capture probe tail and immobilized probes do not form stable duplexes with D-nucleic acids present in the in the sample containing the target nucleic acid. Binding of nucleic acids in the sample directly to immobilized probe or to the tail of the capture probe is reduced or eliminated increasing the sensitivity and/or specificity of the assay.
摘要翻译：本发明提供了通过可以在固定化探针中结合互补L-核酸的L-核酸尾部固定的嵌合捕获探针。捕获探针可用于从样品中捕获靶核酸。捕获探针尾部的L-核酸与固定化探针中的互补L-核酸结合，具有与否则相当于D-核酸的亲和力。然而，捕获探针尾和固定化探针的L-核酸不与存在于含有靶核酸的样品中的D-核酸形成稳定的双链体。将样品中的核酸直接与捕获探针的固定化探针或尾部的结合降低或消除，增加了测定的灵敏度和/或特异性。

10. 发明申请

WO2006138679A3 METHODS FOR QUANTITATIVE ANALYSIS OF A NUCLEIC ACID AMPLIFICATION REACTION 审中-公开
公开(公告)号：WO2006138679A3
公开(公告)日：2006-12-28
申请号：PCT/US2006/023680
申请日：2006-06-14
申请人： GEN-PROBE INCORPORATED , LEE, Richard A. , BECKER, Michael, M. , RESHATOFF, Michael
发明人： LEE, Richard A. , BECKER, Michael, M. , RESHATOFF, Michael
IPC分类号： G06K9/00 , C12Q1/68
摘要： Methods for quantitating an initial amount of a target nucleic acid in a sample which has been subjected to in vitro nucleic acid amplification to produce data that is analyzed by using a Fourier Transform based algorithm are disclosed.

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