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1. 发明申请

WO2014153260A1 METHODS FOR AMPLIFICATION OF NUCLEIC ACIDS ON SOLID SUPPORT 审中-公开
标题翻译：在固体支持物上放大核酸的方法
公开(公告)号：WO2014153260A1
公开(公告)日：2014-09-25
申请号：PCT/US2014/029817
申请日：2014-03-14
申请人： ARNOLD, Lyle, J. , NELSON, Norman, C.
发明人： ARNOLD, Lyle, J. , NELSON, Norman, C.
IPC分类号： C12Q1/68 , C12P19/34 , C12N9/10
CPC分类号： C12P19/34 , C12Q1/6853 , C12Q1/6865 , C12Q2525/186 , C12Q2533/101 , C12Q2565/515 , C12Q2565/537 , C12Q2531/143
摘要： The present invention provides methods for amplifying a nucleic acid from a mixture of nucleic acids utilizing a solid support. Methods are provided utilizing user-defined primer oligonucleotides for directional amplification. Methods are also provided utilizing blocker and displacer oligonucleotides for generating amplified target nucleic acids of defined length. A sample is applied to the solid support and the target nucleic acid within the sample binds a first oligonucleotide affixed to a solid support. A primer sequence containing a target binding region and a polymerase promoter sequence is annealed to the bound target nucleic acid and extended producing a first duplex nucleic acid. The target sequence is removed leaving a first nucleic acid that binds a second oligonucleotide affixed to a solid support. The second oligonucleotide is extended to produce a second duplex nucleic acid that contains a second nucleic acid. The second nucleic acid is amplified by adding a polymerase.
摘要翻译：本发明提供了利用固体支持物从核酸混合物中扩增核酸的方法。使用用于定向扩增的用户定义的引物寡核苷酸提供方法。还提供了利用用于产生具有一定长度的扩增靶核酸的阻断剂和置换寡核苷酸的方法。将样品施加到固体支持物上，并且样品内的靶核酸结合固定在固体支持物上的第一寡核苷酸。包含靶结合区和聚合酶启动子序列的引物序列与结合的靶核酸退火并延长产生第一双链体核酸。去除靶序列，留下结合固定支持物上的第二寡核苷酸的第一核酸。扩展第二寡核苷酸以产生含有第二核酸的第二双链体核酸。通过加入聚合酶扩增第二个核酸。

2. 发明申请

WO2014145138A2 METHODS FOR AMPLIFICATION OF NUCLEIC ACIDS UTILIZING CLAMP OLIGONULEOTIDES 审中-公开
标题翻译：使用钳夹OLIGOULEOTIDES的核酸增殖方法
公开(公告)号：WO2014145138A2
公开(公告)日：2014-09-18
申请号：PCT/US2014/029850
申请日：2014-03-14
申请人： ARNOLD, Lyle, J. , NELSON, Norman, C.
发明人： ARNOLD, Lyle, J. , NELSON, Norman, C.
IPC分类号： C12P19/34
CPC分类号： C12P19/34 , C12Q1/6844 , C12Q2521/501 , C12Q2525/307 , C12Q2549/126
摘要： The present invention provides methods of amplifying a target nucleic acid utilizing a clamp oligonucleotide comprising a first target-binding region on the 3 '-terminus and a second target-binding region on the 5 '-terminus and tether region in between. The tether region may comprise a variety of user-defined sequences or elements that allow for further manipulation of the target nucleic acid. Such as, for example, capture followed by amplification, identification and/or sequencing. The target-binding regions bind to the target nucleic acid, the 3'-terminus functions as a primer to initiate extension across the target nucleic acid sequence and ligation of the gap results in formation of a circularized nucleic acid. This circular template can be used in a variety of processes, including amplification and sequencing.
摘要翻译：本发明提供利用包含3'末端上的第一靶结合区和其间5'末端和系链区之间的第二靶结合区的钳位寡核苷酸扩增靶核酸的方法。系链区域可以包含允许进一步操纵靶核酸的各种用户定义的序列或元件。例如，进行捕获，随后进行扩增，鉴定和/或测序。靶结合区域与靶核酸结合，3'-末端用作引物以引发靶核酸序列的延伸，并且间隙的连接导致形成环化的核酸。该圆形模板可用于各种过程，包括扩增和测序。

3. 发明申请

WO2006093892A2 COMPOSITIONS AND METHODS OF DETECTING AN ANALYTE BY USING A NUCLEIC ACID HYBRIDIZATION SWITCH PROBE 审中-公开
标题翻译：使用核酸杂交开关探针检测分析物的组合物和方法
公开(公告)号：WO2006093892A2
公开(公告)日：2006-09-08
申请号：PCT/US2006/006895
申请日：2006-02-28
申请人： GEN-PROBE INCORPORATED , ARNOLD, Lyle J. Jr. , DAI, Lizhong , BRENTANO, Steven, T. , RUSSELL, James
发明人： ARNOLD, Lyle J. Jr. , DAI, Lizhong , BRENTANO, Steven, T. , RUSSELL, James
CPC分类号： C12Q1/6816 , C12Q2525/197 , C12Q2525/161 , C12Q2565/107 , C12Q2563/131 , C12Q2525/301
摘要： Compositions are described for detecting binding of an analyte to a binding partner attached to a nucleic acid hybridization switch probe that includes first and second arm sequences and a support sequence that is at least partially complementary to both arm sequences, allowing the probe under hybridization conditions to form a first conformation in the absence of the analyte and to form a second conformation in the presence of the analyte, and a label associated with the probe that produces a signal that indicates the conformation of the probe. Methods are described for detecting an analyte that forms a specific binding pair with the binding partner attached to the hybridization switch probe, thereby changing the probe from a first to a second conformation that results in a detectable signal that indicates the presence of the analyte in the sample.
摘要翻译：描述了用于检测分析物与连接至核酸杂交开关探针的结合配偶体的结合的组合物，所述核酸杂交开关探针包括第一和第二臂序列以及与两个臂序列至少部分互补的支持序列，允许探针在杂交条件下在不存在分析物的情况下形成第一构象并在分析物存在下形成第二构象，并且与探针相关联的标记产生指示探针构象的信号。描述了用于检测分析物的方法，所述分析物与附着于杂交开关探针的结合配偶体形成特异性结合配对，由此将探针从第一构型改变为第二构型，其导致可检测信号，其指示分析物存在于样品

4. 发明申请

WO1989002896A1 ACRIDINIUM ESTER LABELLING AND PURIFICATION OF NUCLEOTIDE PROBES 审中-公开
标题翻译：。。。。。。。。。。。。。。。。。。。。。。。
公开(公告)号：WO1989002896A1
公开(公告)日：1989-04-06
申请号：PCT/US1988003361
申请日：1988-10-05
申请人： ML TECHNOLOGY VENTURES, L.P. , ARNOLD, Lyle, John , NELSON, Norman, Charles
发明人： ML TECHNOLOGY VENTURES, L.P.
IPC分类号： C07H21/02
CPC分类号： C07H21/00
摘要： Methods for the construction, labelling and subsequent purification of nucleic acid probes containing primary amines with acridinium esters 4-(2-succinimidyloxycarbonyl-ethyl)phenyl-10-methylacridinium-9-carboxylate fluorosulfonate). The method for attaching acridinium esters to probes uses high (0.1 to 50mM) acridinium ester concentrations achieved using organic solvent in concentrations of 20 % to 80 % by volume, and may be carried out either in solution, or with one or the other of the acridinium ester or the probe suspended in solution. Purification (the separation of labelled probe from unlabelled probe and free label) involves (1) first removing most of the free acridinium ester label from probe using rapid separation techniques, then (2) removing substantially all remaining free label from the probe and separating labelled probe from unlabelled probe, involves specific applications of ion exchange, reverse phase or hydroxyapatite HPLC.

5. 发明申请

WO2012151560A3 METHODS FOR DETECTING NUCLEIC ACID SEQUENCE VARIANTS 审中-公开
标题翻译：检测核酸序列变异体的方法
公开(公告)号：WO2012151560A3
公开(公告)日：2013-03-21
申请号：PCT/US2012036678
申请日：2012-05-04
申请人： BIOCEPT INC , ARNOLD LYLE
发明人： ARNOLD LYLE
IPC分类号： C12Q1/68 , C12N15/11
CPC分类号： C12Q1/6869 , C12Q1/6858 , C12Q2600/112 , C12Q2537/163
摘要： The present invention provides methods for detecting the presence or absence of a nucleic acid variant in a target region. These methods include amplifying the target region with a forward primer and a reverse primer in the presence of a selector blocker. The selector blocker includes a sequence complementary to the target region in the absence of the nucleic acid variant. The methods further include detecting amplification of the target region where amplification of the target region indicates the presence of the nucleic acid variant in the target region. The nucleic acid variant can include deletions, mutations or insertions.
摘要翻译：本发明提供了用于检测目标区域中核酸变体的存在或不存在的方法。这些方法包括在选择剂阻断剂存在下用正向引物和反向引物扩增靶区域。选择剂阻断剂包含在不存在核酸变体时与靶区域互补的序列。所述方法还包括检测目标区域的扩增，其中靶区域的扩增表示靶区域中核酸变体的存在。核酸变体可以包括缺失，突变或插入。

6. 发明申请

WO2012151560A2 METHODS FOR DETECTING NUCLEIC ACID SEQUENCE VARIANTS 审中-公开
标题翻译：检测核酸序列变异体的方法
公开(公告)号：WO2012151560A2
公开(公告)日：2012-11-08
申请号：PCT/US2012/036678
申请日：2012-05-04
申请人： BIOCEPT, INC. , ARNOLD, Lyle
发明人： ARNOLD, Lyle
CPC分类号： C12Q1/6869 , C12Q1/6858 , C12Q2600/112 , C12Q2537/163
摘要： The present invention provides methods for detecting the presence or absence of a nucleic acid variant in a target region. These methods include amplifying the target region with a forward primer and a reverse primer in the presence of a selector blocker. The selector blocker includes a sequence complementary to the target region in the absence of the nucleic acid variant. The methods further include detecting amplification of the target region where amplification of the target region indicates the presence of the nucleic acid variant in the target region. The nucleic acid variant can include deletions, mutations or insertions.
摘要翻译：本发明提供了用于检测目标区域中核酸变体的存在或不存在的方法。这些方法包括在选择剂阻断剂存在下用正向引物和反向引物扩增靶区域。选择剂阻断剂包含在不存在核酸变体时与靶区域互补的序列。所述方法还包括检测目标区域的扩增，其中靶区域的扩增表示靶区域中核酸变体的存在。核酸变体可以包括缺失，突变或插入。

7. 发明申请

WO2012037531A1 CAPTURE PROBES IMMOBILIZABLE VIA L-NUCLEOTIDE TAIL 审中-公开
标题翻译：捕获探针通过L-核核酸尾部可维持
公开(公告)号：WO2012037531A1
公开(公告)日：2012-03-22
申请号：PCT/US2011/052050
申请日：2011-09-16
申请人： Gen-Probe Incorporated , POLLNER, Reinhold , MAJLESSI, Mehrdad , YAMAGATA, Susan , BECKER, Michael, M. , REYNOLDS, Mark , ARNOLD, Lyle
发明人： POLLNER, Reinhold , MAJLESSI, Mehrdad , YAMAGATA, Susan , BECKER, Michael, M. , REYNOLDS, Mark , ARNOLD, Lyle
IPC分类号： C12Q1/68
CPC分类号： C12Q1/707 , C12Q1/6837 , C12Q1/703 , Y02A50/53 , Y02A50/54 , C12Q2525/161
摘要： The invention provides chimeric capture probes immobilizable via an L-nucleic acid tail that can bind to a complementary L-nucleic acid in an immobilized probe. The capture probes are useful for capturing a target nucleic acid from a sample. The L-nucleic acid in the tail of the capture probe bind to the complementary L-nucleic acid in the immobilized probe with similar affinity as would otherwise equivalent D-nucleic acids. However, the L-nucleic acid of the capture probe tail and immobilized probes do not form stable duplexes with D-nucleic acids present in the in the sample containing the target nucleic acid. Binding of nucleic acids in the sample directly to immobilized probe or to the tail of the capture probe is reduced or eliminated increasing the sensitivity and/or specificity of the assay.
摘要翻译：本发明提供了通过可以在固定化探针中结合互补L-核酸的L-核酸尾部固定的嵌合捕获探针。捕获探针可用于从样品中捕获靶核酸。捕获探针尾部的L-核酸与固定化探针中的互补L-核酸结合，具有与否则相当于D-核酸的亲和力。然而，捕获探针尾和固定化探针的L-核酸不与存在于含有靶核酸的样品中的D-核酸形成稳定的双链体。将样品中的核酸直接与捕获探针的固定化探针或尾部的结合降低或消除，增加了测定的灵敏度和/或特异性。

8. 发明申请

WO2022103978A1 PATHOGEN INACTIVATING AND NUCLEIC ACID STABILIZATION MEDIA FOR MICROORGANISM COLLECTION AND TRANSPORT 审中-公开
公开(公告)号：WO2022103978A1
公开(公告)日：2022-05-19
申请号：PCT/US2021/059004
申请日：2021-11-11
申请人： BIOCEPT, INC. , ARNOLD, Lyle
发明人： ARNOLD, Lyle
IPC分类号： C07K14/00 , C07K14/47 , C07K14/78
摘要： The present disclosure provides pathogen inactivating formulations, and methods of use thereof in collecting, transporting and storing biological samples, as well as preventing degradation of and purifying nucleic acids. The disclosure further provides methods of preparing compositions comprising the pathogen inactivating formulation and a biological sample.

9. 发明申请

WO2014145138A3 METHODS FOR AMPLIFICATION OF NUCLEIC ACIDS UTILIZING CLAMP OLIGONULEOTIDES 审中-公开
标题翻译：使用钳夹OLIGOULEOTIDES的核酸增殖方法
公开(公告)号：WO2014145138A3
公开(公告)日：2014-11-13
申请号：PCT/US2014029850
申请日：2014-03-14
申请人： ARNOLD LYLE J , NELSON NORMAN C
发明人： ARNOLD LYLE J , NELSON NORMAN C
IPC分类号： C12Q1/68 , C12M3/00
CPC分类号： C12P19/34 , C12Q1/6844 , C12Q2521/501 , C12Q2525/307 , C12Q2549/126
摘要： The disclosure provides methods of amplifying a target nucleic acid utilizing a clamp oligonucleotide comprising a first target-binding region on the 3' -terminus and a second target-binding region on the 5' -terminus and tether region in between. The tether region may comprise a variety of user-defined sequences or elements that allow for further manipulation of the target nucleic acid. Such as, capture followed by amplification, identification and/or sequencing. The target-binding regions bind to the target nucleic acid, the 3' -terminus functions as a primer to initiate extension across the target nucleic acid sequence and ligation of the gap results in formation of a circularized nucleic acid. This circular template can be used in a variety of processes, including amplification and sequencing.
摘要翻译：本公开提供了利用包含3'末端上的第一靶结合区和其间5'末端和系链区之间的第二靶结合区的钳位寡核苷酸扩增靶核酸的方法。系链区域可以包含允许进一步操纵靶核酸的各种用户定义的序列或元件。例如，捕获后进行扩增，鉴定和/或测序。靶结合区域与靶核酸结合，3'-末端起着引物的作用，以引发靶核酸序列的延伸，并且连接间隙导致形成环化的核酸。该圆形模板可用于各种过程，包括扩增和测序。

10. 发明申请

WO2006093892A3 COMPOSITIONS AND METHODS OF DETECTING AN ANALYTE BY USING A NUCLEIC ACID HYBRIDIZATION SWITCH PROBE 审中-公开
标题翻译：通过使用核酸混合开关探针检测分析物的组合物和方法
公开(公告)号：WO2006093892A3
公开(公告)日：2006-12-14
申请号：PCT/US2006006895
申请日：2006-02-28
申请人： GEN PROBE INC , ARNOLD LYLE J JR , DAI LIZHONG , BRENTANO STEVEN T , RUSSELL JAMES
发明人： ARNOLD LYLE J JR , DAI LIZHONG , BRENTANO STEVEN T , RUSSELL JAMES
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6816 , C12Q2525/197 , C12Q2525/161 , C12Q2565/107 , C12Q2563/131 , C12Q2525/301
摘要： Compositions are described for detecting binding of an analyte to a binding partner attached to a nucleic acid hybridization switch probe that includes first and second arm sequences and a support sequence that is at least partially complementary to both arm sequences, allowing the probe under hybridization conditions to form a first conformation in the absence of the analyte and to form a second conformation in the presence of the analyte, and a label associated with the probe that produces a signal that indicates the conformation of the probe. Methods are described for detecting an analyte that forms a specific binding pair with the binding partner attached to the hybridization switch probe, thereby changing the probe from a first to a second conformation that results in a detectable signal that indicates the presence of the analyte in the sample.
摘要翻译：描述了用于检测分析物与连接到核酸杂交开关探针的结合配偶体的结合的组合物，其包含第一和第二臂序列以及与两个臂序列至少部分互补的支持序列，允许探针在杂交条件下在不存在分析物的情况下形成第一构象，并在分析物存在下形成第二构象，以及与探针相关联的标记，其产生指示探针构象的信号。描述了用于检测与附着到杂交开关探针的结合配偶体形成特异性结合对的分析物的方法，从而将探针从第一至第二构象改变，从而导致可检测的信号，其指示分析物在样品。

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