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1. 发明申请

WO2008108843A2 METHODS AND KITS FOR AMPLIFYING DNA 审中-公开
标题翻译：扩增DNA的方法和试剂盒
公开(公告)号：WO2008108843A2
公开(公告)日：2008-09-12
申请号：PCT/US2007/063103
申请日：2007-03-01
申请人： GEN-PROBE INCORPORATED , BECKER, Michael, M. , LAM, Wai-Chung , LIVEZEY, Kristin, W. , BRENTANO, Steven, T. , KOLK, Daniel, P. , SCHRODER, Astrid, R.W.
发明人： BECKER, Michael, M. , LAM, Wai-Chung , LIVEZEY, Kristin, W. , BRENTANO, Steven, T. , KOLK, Daniel, P. , SCHRODER, Astrid, R.W.
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6865 , C12Q2533/101 , C12Q2525/186 , C12Q2521/107
摘要： Novel methods of synthesizing multiple copies of a target nucleic acid sequence which are autocatalytic are disclosed (/. e., able to cycle automatically without the need to modify reaction conditions such as temperature, pH, or ionic strength and using the product of one cycle in the next one). In particular, methods of nucleic acid amplification are disclosed which are robust and efficient, while reducing the appearance of side-products. In general, the methods use priming oligonucleotides that target only one sense of a target nucleic acid, a promoter oligonucleotide modified to prevent polymerase extension from its 3 '-terminus and, optionally, a means for terminating a primer extension reaction, to amplify RNA or DNA molecules in vitro, while reducing or substantially eliminating the formation of side-products. The disclosed methods minimizes or substantially eliminate the emergence of side-products, thus providing ahigh level of specificity. Furthermore, the appearance of side-products can complicate the analysis of the amplification reaction by various molecular detection techniques. The disclosed methods minimize or substantially eliminate this problem, thus providing enhanced levels of sensitivity.
摘要翻译：公开了合成自催化的靶核酸序列的多个拷贝的新方法（即，能够自动循环而不需要修改诸如温度，pH或离子的反应条件力量和使用下一个周期的产品）。具体而言，公开了稳定且有效的核酸扩增方法，同时减少副产物的出现。一般而言，所述方法使用仅靶向靶核酸的一种意义的引发寡核苷酸，经修饰以阻止聚合酶从其3'-末端延伸的启动子寡核苷酸，以及任选地终止引物延伸反应的手段，以扩增RNA或体外DNA分子，同时减少或基本消除副产物的形成。所公开的方法最小化或基本上消除了副产物的出现，从而提供了高水平的特异性。此外，副产物的出现可能会使各种分子检测技术对扩增反应的分析复杂化。所公开的方法最小化或基本上消除了这个问题，因此提供了增强的灵敏度水平。

2. 发明申请

WO2009105592A3 COMPOSITIONS AND METHODS FOR DETECTION OF PROPIONIBACTERIUM ACNES NUCLEIC ACID 审中-公开
标题翻译：检测丙酸杆菌核酸的组合物和方法
公开(公告)号：WO2009105592A3
公开(公告)日：2009-12-30
申请号：PCT/US2009034592
申请日：2009-02-19
申请人： HOGAN JAMES J , LIVEZEY KRISTIN W , KAPLAN SHANNON K , BUNGO JENNIFER J
发明人： HOGAN JAMES J , LIVEZEY KRISTIN W , KAPLAN SHANNON K , BUNGO JENNIFER J
IPC分类号： C12Q1/68 , C07H21/04
CPC分类号： C12Q1/689 , C12Q2600/156
摘要： Methods for amplifying and detecting Propionibacterium acnes nucleic acid by targeting specific sequences in 16S rRNA, 23S rRNA, or DNA encoding 16S rRNA or 23S rRNA are disclosed. Nucleic acid oligonucleotide sequence compositions specific for P. acnes nucleic acid sequences in 16S or 23S rRNA or DNA encoding 16S or 23S rRNA sequences are disclosed, which are useful for amplification oligonucletides, capture probes in sample preparation, and probes for detection of P. acnes nucleic acid sequences.
摘要翻译：公开了通过靶向16S rRNA，23S rRNA或编码16S rRNA或23S rRNA的DNA中的特定序列来扩增和检测痤疮丙酸杆菌核酸的方法。公开了对16S或23S rRNA中的痤疮丙酸杆菌核酸序列或编码16S或23S rRNA序列的DNA特异性的核酸寡核苷酸序列组成，其可用于扩增寡核苷酸，样品制备中的捕获探针和用于检测痤疮丙酸杆菌的探针核酸序列。

3. 发明申请

WO2006026388A2 SINGLE-PRIMER NUCLEIC ACID AMPLIFICATION METHODS 审中-公开
标题翻译：单一核酸核酸扩增方法
公开(公告)号：WO2006026388A2
公开(公告)日：2006-03-09
申请号：PCT/US2005/030329
申请日：2005-08-26
申请人： GEN-PROBE INCORPORATED , BECKER, Michael, M. , LAM, Wai-Chung , LIVEZEY, Kristin, W. , BRENTANO, Steven, T. , KOLK, Daniel, P. , SCHRODER, Astrid, R.W.
发明人： BECKER, Michael, M. , LAM, Wai-Chung , LIVEZEY, Kristin, W. , BRENTANO, Steven, T. , KOLK, Daniel, P. , SCHRODER, Astrid, R.W.
IPC分类号： C12Q1/68 , C12P19/34
CPC分类号： C12P19/34 , C12Q1/6865 , C12Q2537/163 , C12Q2521/325 , C12Q2521/107 , C12Q2525/186 , C12Q2533/101 , C12Q2525/143
摘要： The present invention is directed to novel methods of synthesizing multiple copies of a target nucleic acid sequence which are autocatalytic ( i.e ., able to cycle automatically without the need to modify reaction conditions such as temperature, pH, or ionic strength and using the product of one cycle in the next one). In particular, the present invention discloses a method of nucleic acid amplification which is robust and efficient, while reducing the appearance of side-products. The method uses only one primer, the "priming oligonucleotide," a 3'blocked promoter oligonucleotide and optionally, a means for terminating a primer extension reaction, to amplify RNA or DNA molecules in vitro , while reducing or eliminating the formation of side-products. The method of the present invention minimizes or eliminates the emergence of side-products, thus providing a high level of specificity. Furthermore, the appearance of side-products can complicate the analysis of the amplification reaction by various molecular detection techniques. The present invention minimizes or eliminates this problem, thus providing an enhanced level of sensitivity.
摘要翻译：本发明涉及合成多个拷贝的目标核酸序列的新方法，所述靶核酸序列是自动催化的（<

4. 发明申请

WO2010151566A1 COMPOSITIONS AND METHODS FOR DETECTING NUCLEIC ACID FROM MOLLICUTES 审中-公开
标题翻译：用于检测来自麦芽糖的核酸的组合物和方法
公开(公告)号：WO2010151566A1
公开(公告)日：2010-12-29
申请号：PCT/US2010/039592
申请日：2010-06-23
申请人： GEN-PROBE INCORPORATED , KAPLAN, Shannon, K. , LIVEZEY, Kristin, W. , BECKER, Michael, M. , HOGAN, James, D.
发明人： KAPLAN, Shannon, K. , LIVEZEY, Kristin, W. , BECKER, Michael, M. , HOGAN, James, D.
IPC分类号： C12Q1/68
CPC分类号： C12Q1/689 , C12Q2600/16
摘要： Compositions, reaction mixtures, kits and methods used in amplifying and detecting nucleic acids from various species of the class Mollicutes. In certain aspects and embodiments, particular regions of the 23S rRNA or gene encoding said rRNA have been identified as preferred targets for nucleic acid amplification reactions of a sample suspected containing at least one species of Mollicutes. Some oligomers comprise tag regions. Some oligomers comprise target closing regions, promoter sequences, and/or binding moieties, such as homopolymeric and heteropolymeric nucleotide sequences. Samples can be from any source suspected of containing a species of the class Mollicutes. Preferred sample sources include bioreactors, cell lines, cell culture wares and pharmaceutical manufacturing wares, to name a few.
摘要翻译：用于扩增和检测来自Mollicutes类的各种物种的核酸的组合物，反应混合物，试剂盒和方法。在某些方面和实施方案中，编码所述rRNA的23S rRNA或基因的特定区域已被鉴定为怀疑含有至少一种Mollicutes的样品的核酸扩增反应的优选靶标。一些低聚物包括标签区。一些寡聚体包含靶闭合区，启动子序列和/或结合部分，例如均聚和杂聚核苷酸序列。样品可以来自怀疑含有Mollicutes类物种的任何来源。优选的样品来源包括生物反应器，细胞系，细胞培养物和药物制造商品等等。

5. 发明申请

WO2010126913A1 METHODS AND KITS FOR USE IN THE SELECTIVE AMPLIFICATION OF TARGET SEQUENCES 审中-公开
标题翻译：用于选择性扩增目标序列的方法和工具
公开(公告)号：WO2010126913A1
公开(公告)日：2010-11-04
申请号：PCT/US2010/032631
申请日：2010-04-27
申请人： GEN-PROBE INCORPORATED , BECKER, Michael, M. , LIVEZEY, Kristin, W.
发明人： BECKER, Michael, M. , LIVEZEY, Kristin, W.
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6848 , C12Q1/6865 , C12Q2531/143 , C12Q2525/301
摘要： The present invention provides nucleic acid amplification methods that desirably reduce or eliminate false positive amplification signals resulting from contaminating biological material, e.g., nucleic acid, that may be present in one or more reagents used in an amplification reaction and/or that may be present in the environment in which an amplification reaction is performed. The invention offers the further advantage of requiring less stringent purification and/or sterility efforts than conventionally needed in order to ensure that enzymes and other reagents used in amplification reactions, and the environment in which an amplification reaction is performed, are free of bacterial or other nucleic acid contamination that may yield false positive results.
摘要翻译：本发明提供了核酸扩增方法，其期望地减少或消除由污染生物材料（例如核酸）产生的假阳性扩增信号，所述核酸可以存在于用于扩增反应的一种或多种试剂中和/或可存在于进行扩增反应的环境。本发明提供了进一步的优点，其需要比常规所需的更少的纯化和/或不育努力，以确保用于扩增反应的酶和其它试剂以及进行扩增反应的环境不含细菌或其它可能产生假阳性结果的核酸污染物。

6. 发明申请

WO2010078374A1 COMPOSITIONS, KITS AND RELATED METHODS FOR THE DETECTION AND/OR MONITORING OF LISTERIA 审中-公开
标题翻译：用于检测和/或监测LISTERIA的组合物，试剂盒和相关方法
公开(公告)号：WO2010078374A1
公开(公告)日：2010-07-08
申请号：PCT/US2009/069746
申请日：2009-12-29
申请人： GEN-PROBE INCORPORATED , RESHATOFF, Michael R. , LIVEZEY, Kristin W. , HOGAN, James J.
发明人： RESHATOFF, Michael R. , LIVEZEY, Kristin W. , HOGAN, James J.
IPC分类号： C12Q1/68
CPC分类号： C12Q1/689 , C12Q1/6816 , C12Q1/6865
摘要： Provided are compositions, kits, and methods for the identification of Listeria . In certain aspects and embodiments, the compositions, kits, and methods may provide improvements in relation to specificity, sensitivity, and speed of detection.
摘要翻译：提供用于鉴定李斯特菌的组合物，试剂盒和方法。在某些方面和实施方案中，组合物，试剂盒和方法可以提供与特异性，灵敏度和检测速度有关的改进。

7. 发明申请

WO2007134208A2 COMPOSITIONS AND METHODS TO DETECT ENTEROCOCCI NUCLEIC ACID 审中-公开
标题翻译：检测肠内核酸的组成和方法
公开(公告)号：WO2007134208A2
公开(公告)日：2007-11-22
申请号：PCT/US2007/068735
申请日：2007-05-11
申请人： GEN-PROBE INCORPORATED , POLLNER, Reinhold B. , LIVEZEY, Kristin W.
发明人： POLLNER, Reinhold B. , LIVEZEY, Kristin W.
CPC分类号： C12Q1/689
摘要： The disclosed invention includes nucleic acid oligomers that may be used as amplification oligomers, including primers, capture probes for sample preparation, and detection probes for detection of indicator enterococci 23S rRNA sequences in samples by using methods of specific nucleic acid amplification and detection.
摘要翻译：所公开的发明包括可用作扩增寡聚体的核酸寡聚体，包括引物，用于样品制备的捕获探针和用于通过特异性核酸扩增和检测方法检测样品中指示性肠球菌23S rRNA序列的检测探针。

8. 发明申请

WO2008108843A3 METHODS AND KITS FOR AMPLIFYING DNA 审中-公开
标题翻译：用于扩增DNA的方法和工具
公开(公告)号：WO2008108843A3
公开(公告)日：2008-10-30
申请号：PCT/US2007063103
申请日：2007-03-01
申请人： GEN PROBE INC , BECKER MICHAEL M , LAM WAI-CHUNG , LIVEZEY KRISTIN W , BRENTANO STEVEN T , KOLK DANIEL P , SCHRODER ASTRID R W
发明人： BECKER MICHAEL M , LAM WAI-CHUNG , LIVEZEY KRISTIN W , BRENTANO STEVEN T , KOLK DANIEL P , SCHRODER ASTRID R W
IPC分类号： C12Q1/68 , C07H21/02
CPC分类号： C12Q1/6865 , C12Q2533/101 , C12Q2525/186 , C12Q2521/107
摘要： Novel methods of synthesizing multiple copies of a target nucleic acid sequence which are autocatalytic are disclosed (/. e., able to cycle automatically without the need to modify reaction conditions such as temperature, pH, or ionic strength and using the product of one cycle in the next one). In particular, methods of nucleic acid amplification are disclosed which are robust and efficient, while reducing the appearance of side-products. In general, the methods use priming oligonucleotides that target only one sense of a target nucleic acid, a promoter oligonucleotide modified to prevent polymerase extension from its 3 '-terminus and, optionally, a means for terminating a primer extension reaction, to amplify RNA or DNA molecules in vitro, while reducing or substantially eliminating the formation of side-products. The disclosed methods minimizes or substantially eliminate the emergence of side-products, thus providing ahigh level of specificity. Furthermore, the appearance of side-products can complicate the analysis of the amplification reaction by various molecular detection techniques. The disclosed methods minimize or substantially eliminate this problem, thus providing enhanced levels of sensitivity.
摘要翻译：公开了合成自动催化的靶核酸序列的多个拷贝的新方法（例如，能够自动循环而不需要修饰反应条件如温度，pH或离子强度，并且使用一个循环的产物在下一个）。特别地，公开了鲁棒和有效的核酸扩增方法，同时减少副产物的外观。通常，所述方法使用仅靶向靶核酸的引物寡核苷酸，修饰以阻止聚合酶从其3'末端延伸的启动子寡核苷酸，以及任选的终止引物延伸反应的手段以扩增RNA或 DNA分子在体外，同时减少或基本上消除副产物的形成。所公开的方法最小化或基本上消除副产物的出现，从而提供高水平的特异性。此外，副产物的出现可能通过各种分子检测技术使扩增反应的分析复杂化。所公开的方法最小化或基本上消除了该问题，从而提供了增强的灵敏度水平。

9. 发明申请

WO2007146154A1 TAGGED OLIGONUCLEOTIDES AND THEIR USE IN NUCLEIC ACID AMPLIFICATION METHODS 审中-公开
标题翻译：标记的寡核苷酸及其在核酸扩增方法中的应用
公开(公告)号：WO2007146154A1
公开(公告)日：2007-12-21
申请号：PCT/US2007/013553
申请日：2007-06-06
申请人： GEN-PROBE INCORPORATED , BECKER, Michael, M. , LIVEZEY, Kristin, W. , LAM, Wai-Chung
发明人： BECKER, Michael, M. , LIVEZEY, Kristin, W. , LAM, Wai-Chung
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6853 , C12Q1/6848 , C12Q1/6865 , C12Q2525/301 , C12Q2525/186 , C12Q2525/155 , C12Q2525/161
摘要： The present invention provides nucleic acid amplification methods that desirably reduce or eliminate false positive amplification signals resulting from contaminating biological material, e.g., nucleic acid, that may be present in one or more reagents used in an amplification reaction and/or that may be present in the environment in which an amplification reaction is performed. The invention offers the further advantage of requiring less stringent purification and/or sterility efforts than conventionally needed in order to ensure that enzymes and other reagents used in amplification reactions, and the environment in which an amplification reaction is performed, are free of bacterial or other nucleic acid contamination that may yield false positive results.
摘要翻译：本发明提供了核酸扩增方法，其期望地减少或消除由可能存在于扩增反应中使用的一种或多种试剂中的生物材料例如核酸引起的假阳性扩增信号和/或可存在于进行扩增反应的环境。本发明提供了进一步的优点，即需要比常规所需的更少的纯化和/或不育努力，以确保用于扩增反应的酶和其它试剂以及进行扩增反应的环境中没有细菌或其它可能产生假阳性结果的核酸污染。

10. 发明申请

WO2006026388A3 SINGLE-PRIMER NUCLEIC ACID AMPLIFICATION METHODS 审中-公开
标题翻译：单一核酸核酸扩增方法
公开(公告)号：WO2006026388A3
公开(公告)日：2007-01-18
申请号：PCT/US2005030329
申请日：2005-08-26
申请人： GEN PROBE INC , BECKER MICHAEL M , LAM WAI-CHUNG , LIVEZEY KRISTIN W , BRENTANO STEVEN T , KOLK DANIEL P , SCHRODER ASTRID R W
发明人： BECKER MICHAEL M , LAM WAI-CHUNG , LIVEZEY KRISTIN W , BRENTANO STEVEN T , KOLK DANIEL P , SCHRODER ASTRID R W
IPC分类号： C12P19/34 , C07H21/04
CPC分类号： C12P19/34 , C12Q1/6865 , C12Q2537/163 , C12Q2521/325 , C12Q2521/107 , C12Q2525/186 , C12Q2533/101 , C12Q2525/143
摘要： The present invention is directed to novel methods of synthesizing multiple copies of a target nucleic acid sequence which are autocatalytic (i.e., able to cycle automatically without the need to modify reaction conditions such as temperature, pH, or ionic strength and using the product of one cycle in the next one). In particular, the present invention discloses a method of nucleic acid amplification which is robust and efficient, while reducing the appearance of side-products. The method uses only one primer, the "priming oligonucleotide," a 3'blocked promoter oligonucleotide and optionally, a means for terminating a primer extension reaction, to amplify RNA or DNA molecules in vitro, while reducing or eliminating the formation of side-products. The method of the present invention minimizes or eliminates the emergence of side-products, thus providing a high level of specificity. Furthermore, the appearance of side-products can complicate the analysis of the amplification reaction by various molecular detection techniques. The present invention minimizes or eliminates this problem, thus providing an enhanced level of sensitivity.
摘要翻译：本发明涉及合成目标核酸序列的多个拷贝的新型方法，所述目标核酸序列是自动催化的（即，能够自动循环而不需要修饰诸如温度，pH或离子强度的反应条件，并且使用一种循环在下一个）。特别地，本发明公开了一种鲁棒有效的核酸扩增方法，同时减少副产物的外观。该方法仅使用一种引物“引物寡核苷酸”，3'封闭的启动子寡核苷酸和任选的终止引物延伸反应的手段，以体外扩增RNA或DNA分子，同时减少或消除副产物的形成。本发明的方法使副产物的出现最小化或消除，从而提供高水平的特异性。此外，副产物的出现可能通过各种分子检测技术使扩增反应的分析复杂化。本发明使这个问题最小化或消除了这一点，从而提供了增强的灵敏度。

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