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1. 发明申请

WO2009131524A1 PREPARATION OF LIQUID MIXTURES 审中-公开
标题翻译：液体混合物的制备
公开(公告)号：WO2009131524A1
公开(公告)日：2009-10-29
申请号：PCT/SE2009/050399
申请日：2009-04-20
申请人： GE HEALTHCARE BIO-SCIENCES AB , BJÖRKESTEN, Lennart , CARREDANO, Enrique , MALMQUIST, Gunnar , RODRIGO, Gustav , STAFSTRÖM, Nils
发明人： BJÖRKESTEN, Lennart , CARREDANO, Enrique , MALMQUIST, Gunnar , RODRIGO, Gustav , STAFSTRÖM, Nils
IPC分类号： G01N30/34 , B01D15/12 , B01J39/00 , B01J41/00
CPC分类号： B01D15/12 , B01D15/166 , B01D15/361 , G01N30/34 , G01N2030/347
摘要： A method of determining the relative component proportions of at least one each of: a buffer; an acid or a base; a solvent; and optionally a salt, for providing a liquid mix- ture of pre-defined pH and ionic strength, wherein the relative component proportions are determined using the equation of Debye-Hckel, wherein the ion size parameter α in the Debye-Hckelequation is determined as the weighted mean ion size of all species contributing to the ionic strength of the liquid mixture, and wherein the ionic strength of each species is used as weighting parameter. The present method is also applicable in a method of providing a liquid mixture. Further there is provided a buffer preparation device.
摘要翻译：一种确定缓冲器中的至少一个的相对分量比例的方法; 酸或碱; 溶剂; 和任选的盐，用于提供预定义的pH和离子强度的液体混合物，其中相对组分比例使用Debye-Hckel等式确定，其中Debye-Hckelequation中的离子尺寸参数a被确定为有助于液体混合物的离子强度的所有物质的加权平均离子尺寸，并且其中每种物质的离子强度用作加权参数。本方法也适用于提供液体混合物的方法。此外，提供了一种缓冲液制备装置。

2. 发明申请

WO2011162666A1 METHOD OF PREPARING LIQUID MIXTURES 审中-公开
标题翻译：制备液体混合物的方法
公开(公告)号：WO2011162666A1
公开(公告)日：2011-12-29
申请号：PCT/SE2011/050563
申请日：2011-05-05
申请人： GE HEALTHCARE BIO-SCIENCES AB , ANDREI, Catalin , BERG, Mikael , BLANK, Torbjörn , CARREDANO, Enrique , EKSTRÖM, Karl , KARLSSON, Tomas, M. , LYNGÅ, Jan-Erik , NORDBERG, Roger , RODRIGO, Gustav , SANDEGREN, Henrik
发明人： ANDREI, Catalin , BERG, Mikael , BLANK, Torbjörn , CARREDANO, Enrique , EKSTRÖM, Karl , KARLSSON, Tomas, M. , LYNGÅ, Jan-Erik , NORDBERG, Roger , RODRIGO, Gustav , SANDEGREN, Henrik
IPC分类号： G05D11/08 , B01F15/04 , G01N30/34 , G01N30/88 , G05D11/13
CPC分类号： F17D3/00 , G01N30/34 , G05D21/02 , Y10T137/0329
摘要： A method of preparing a mixed liquid flow having predetermined characteristics, including a predetermined value of a first property and a predetermined value of a second property, comprising the steps of: a) providing a first set of at least one liquid flow each having a different first value of the first property; b) providing a second set of at least one liquid flow each having a different second value of the first property; c) providing a third set of at least one liquid flow of solvent; d) combining the provided liquid flows; and e)varying at least one of the liquid flows of the first and second sets and at least one liquid flow of the third set to adjust the first property and the second property to their respective predetermined values in the resulting mixed liquid flow.
摘要翻译：一种制备具有预定特性的混合液流的方法，包括第一特性的预定值和第二特性的预定值，包括以下步骤：a）提供第一组至少一个液体流，每组具有不同的第一财产的第一价值; b）提供第二组至少一个液体流，每个具有第一属性的不同的第二值; c）提供第三组至少一种液体的溶剂流; d）组合提供的液体流; 以及e）改变所述第一和第二组的液体流中的至少一个以及所述第三组的至少一个液体流，以将所述第一特性和所述第二特性调整到所得混合液体流中其各自的预定值。

3. 发明申请

WO2011037530A1 METHOD AND SYSTEM FOR PREPARATION OF LIQUID MIXTURES 审中-公开
标题翻译：液体混合物的制备方法和系统
公开(公告)号：WO2011037530A1
公开(公告)日：2011-03-31
申请号：PCT/SE2010/051023
申请日：2010-09-23
申请人： GE HEALTHCARE BIO-SCIENCES AB , BLANK, Torbjörn , CARREDANO, Enrique , EKSTRÖM, Karl , LYNGÅ, Jan-Erik , NORDBERG, Roger , PETERS, Mike , RODRIGO, Gustav , SANDEGREN, Henrik , STACK, Lisa
发明人： BLANK, Torbjörn , CARREDANO, Enrique , EKSTRÖM, Karl , LYNGÅ, Jan-Erik , NORDBERG, Roger , PETERS, Mike , RODRIGO, Gustav , SANDEGREN, Henrik , STACK, Lisa
IPC分类号： G05D11/02 , B01F15/04 , G01N30/34 , G05D11/08 , B01D15/16 , G01N30/88
CPC分类号： B01D15/166 , B01F15/0022 , B01F15/00227 , G01N30/34 , G01N2030/347 , G05D11/132
摘要： A method for preparing a liquid flow having pre-defined characteristics by mixing liquid flows of at least two different component stock solutions with each other comprises the steps of determining a selected property value for one or more of the stock solutions by sensing, in a flow of each stock solution separately, at least one characteristic related to the property value for the stock solution, and based on the determined property value or values, mixing the stock solution flows in mixing ratios giving the desired mixed liquid flow. A system for carrying out the method comprises sensor means and a control unit, wherein the control unit is arranged to evaluate the characteristics sensed by the sensor means and provide relative ratios of stock solutions required to obtain a mixed liquid flow having the pre-defined characteristics.
摘要翻译：通过混合至少两种不同组分原料溶液的液体流相互混合制备具有预定特性的液体流的方法包括以下步骤：通过在流体中感测来确定一种或多种储备溶液的选定的特性值分别存储与储备溶液的特性值相关的至少一个特征，并且基于所确定的特性值或值，以提供所需混合液体流的混合比混合储备液流。用于执行该方法的系统包括传感器装置和控制单元，其中控制单元布置成评估由传感器装置感测到的特性，并提供获得具有预定特性的混合液流所需的储备溶液的相对比例。

4. 发明申请

WO2012074463A1 AFFINITY CHROMATOGRAPHY MATRIX 审中-公开
标题翻译：亲属色谱矩阵
公开(公告)号：WO2012074463A1
公开(公告)日：2012-06-07
申请号：PCT/SE2011/051432
申请日：2011-11-28
申请人： GE HEALTHCARE BIO-SCIENCES AB , BJÖRKMAN, Tomas , RODRIGO, Gustav
发明人： BJÖRKMAN, Tomas , RODRIGO, Gustav
IPC分类号： B01D15/38 , B01J20/281 , C07K1/22 , C07K14/31 , C12N15/03
CPC分类号： C07K1/22 , B01D15/3809 , B01J20/286 , B01J20/3208 , B01J20/3242 , B01J20/3274 , C07K16/065
摘要： The present invention relates to a method of separating one or more immunoglobulin containing proteins from a liquid. The method includes first contacting the liquid with a separation matrix comprising ligands immobilised to a support; allowing the immunoglobulin containing proteins to adsorb to the matrix by interaction with the ligands; followed by an optional step of washing the adsorbed immunoglobulin containing proteins; and recovering said immunoglobulin containing proteins by contacting the matrix with an eluent which releases the proteins. The method improves upon previous separation methods in that each of the ligands comprises one or more of a protein A domain (E, D, A, B, C), or protein Z, or a functional variant thereof, with at least one of the monomers having a substitution of the C-terminal most proline residue after the third alpha-helix.
摘要翻译：本发明涉及从液体中分离一种或多种含免疫球蛋白的蛋白质的方法。该方法包括首先使液体与包含固定在载体上的配体的分离基质接触; 允许含有免疫球蛋白的蛋白质通过与配体的相互作用吸附到基质上; 随后是洗涤吸附的含有蛋白质的免疫球蛋白的任选步骤; 并通过使基质与释放蛋白质的洗脱液接触来回收所述含有免疫球蛋白的蛋白质。该方法改进了以前的分离方法，因为每种配体包含蛋白A结构域（E，D，A，B，C）或蛋白质Z或其功能变体中的一种或多种，其中至少一种在第三个α-螺旋之后具有C末端最多脯氨酸残基取代的单体。

5. 发明申请

WO2012087231A1 AFFINITY CHROMATOGRAPHY MATRIX 审中-公开
标题翻译：亲属色谱矩阵
公开(公告)号：WO2012087231A1
公开(公告)日：2012-06-28
申请号：PCT/SE2011/051537
申请日：2011-12-19
申请人： GE HEALTHCARE BIO-SCIENCES AB , BJÖRKMAN, Tomas , NORÉN, Bjorn , RODRIGO, Gustav , VASIC, Jelena , ÅBERG, Per-Mikael
发明人： BJÖRKMAN, Tomas , NORÉN, Bjorn , RODRIGO, Gustav , VASIC, Jelena , ÅBERG, Per-Mikael
IPC分类号： B01D15/38 , B01J20/281 , C07K1/22 , C07K14/31 , C12N15/03
CPC分类号： C07K16/1271 , B01D15/168 , B01D15/3809 , B01J20/286 , B01J20/289 , B01J20/3212 , B01J20/3219 , B01J20/3274 , C07K1/22 , C07K14/31
摘要： The present invention relates to a method of separating one or more immunoglobulin containing proteins from a liquid. The method includes first contacting the liquid with a separation matrix comprising ligands immobilised to a support; allowing the immunoglobulin containing proteins to adsorb to the matrix by interaction with the ligands; followed by an optional step of washing the matrix containing the immunoglobulin containing proteins adsorbed thereon; and recovering said immunoglobulin containing proteins by contacting the matrix with an eluent which releases the proteins. The method improves upon previous separation methods in that each of the ligands comprises one or more of a protein A domain (E, D, A, B, C), or protein Z, or a functional variant thereof, with at least one of the monomers having a substitution of the Asparagineor Histidine at the position corresponding to H18 of B domain of Protein A or Protein Z, and wherein the ligand provides an increase in elution pH compared to non-substituted ligand.
摘要翻译：本发明涉及从液体中分离一种或多种含免疫球蛋白的蛋白质的方法。该方法包括首先使液体与包含固定在载体上的配体的分离基质接触; 允许含有免疫球蛋白的蛋白质通过与配体的相互作用吸附到基质上; 随后是洗涤含有其上吸附有蛋白质的蛋白质的基质的任选步骤; 并通过使基质与释放蛋白质的洗脱液接触来回收所述含有免疫球蛋白的蛋白质。该方法改进了先前的分离方法，因为每种配体包含蛋白A结构域（E，D，A，B，C）或蛋白质Z或其功能变体中的一种或多种，其中至少一种在相应于蛋白A或蛋白Z的B结构域的H18的位置处取代天门冬酰胺组氨酸的单体，并且其中与非取代的配体相比，配体提供了洗脱pH的增加。

6. 发明申请

WO2012087230A1 AFFINITY CHROMATOGRAPHY MATRIX 审中-公开
标题翻译：亲属色谱矩阵
公开(公告)号：WO2012087230A1
公开(公告)日：2012-06-28
申请号：PCT/SE2011/051536
申请日：2011-12-19
申请人： GE HEALTHCARE BIO-SCIENCES AB , BJÖRKMAN, Tomas , MONIE, Elin , RODRIGO, Gustav
发明人： BJÖRKMAN, Tomas , MONIE, Elin , RODRIGO, Gustav
IPC分类号： B01D15/38 , B01J20/281 , C07K1/22 , C07K14/31 , C12N15/03
CPC分类号： C07K1/22 , B01D15/3809 , B01J20/286 , B01J20/289 , B01J20/3212 , B01J20/3219 , B01J20/3274 , C07K14/31 , C07K14/745
摘要： The present invention relates to a method of separating one or more immunoglobulin containing proteins from a liquid. The method includes first contacting the liquid with a separation matrix comprising ligands immobilised to a support; allowing the immunoglobulin containing proteins to adsorb to the matrix by interaction with the ligands; followed by an optional step of washing the matrix containing the immunoglobulin containing proteins adsorbed thereon; and recovering said immunoglobulin containing proteins by contacting the matrix with an eluent which releases the proteins. The method improves upon previous separation methods in that each of the ligands comprises one or more of a protein A domain (E, D, A, B, C), or protein Z, or a functional variant thereof, with at least one of the monomers having a substitution of the Asparagine at the position corresponding to N28 of B domain of Protein A or Protein Z, and wherein the ligand provides an increase in elution pH compared to non-substituted ligand.
摘要翻译：本发明涉及从液体中分离一种或多种含免疫球蛋白的蛋白质的方法。该方法包括首先使液体与包含固定在载体上的配体的分离基质接触; 允许含有免疫球蛋白的蛋白质通过与配体的相互作用吸附到基质上; 随后是洗涤含有其上吸附有蛋白质的蛋白质的基质的任选步骤; 并通过使基质与释放蛋白质的洗脱液接触来回收所述含有免疫球蛋白的蛋白质。该方法改进了以前的分离方法，因为每种配体包含蛋白A结构域（E，D，A，B，C）或蛋白质Z或其功能变体中的一种或多种，其中至少一种在对应于蛋白A或蛋白Z的B结构域的N28的位置处取代天门冬酰胺的单体，并且其中与未取代的配体相比，配体提供了洗脱pH的增加。

7. 发明申请

WO2008039141A1 CHROMATOGRAPHY LIGAND COMPRISING DOMAIN C FROM STAPHYLOCCOCUS AUREUS PROTEIN A FOR ANTIBODY ISOLATION 审中-公开
标题翻译：含有葡萄球菌A蛋白A的抗体分离区域C的色谱配体
公开(公告)号：WO2008039141A1
公开(公告)日：2008-04-03
申请号：PCT/SE2007/000862
申请日：2007-09-27
申请人： GE HEALTHCARE BIO-SCIENCES AB , HALL, Martin , LARSSON, Sture , MURANYI, Andreas , RODRIGO, Gustav , ZOU, Jinyu , ÅBERG, Per-Mikael
发明人： HALL, Martin , LARSSON, Sture , MURANYI, Andreas , RODRIGO, Gustav , ZOU, Jinyu , ÅBERG, Per-Mikael
IPC分类号： B01D15/38 , C07K1/22 , C07K14/31 , G01N33/53
CPC分类号： B01J20/24 , B01D15/3809 , B01J20/28019 , B01J20/285 , B01J20/286 , B01J20/289 , B01J20/3212 , B01J20/3219 , B01J20/3274 , B01J20/3293 , B01J2220/52 , B01J2220/54 , C07K1/22 , C07K14/31 , C07K16/00 , C07K17/00 , C07K17/10 , C07K2317/55
摘要： The present invention relates to a chromatography ligand, which comprises Domain C from Staphylococcus protein A (SpA), or a functional fragment or variant thereof. The chromatography ligand presents an advantageous capability of withstanding harsh cleaning in place (CIP) conditions, and is capable of binding Fab fragments of antibodies. The ligand may be provided with a terminal coupling group, such as arginine or cysteine, to facilitate its coupling to an insoluble carrier such as beads or a membrane. The invention also relates to a process of using the ligand in isolation of antibodies, and to a purification protocol which may include washing steps and/or re-generation with alkali.
摘要翻译：本发明涉及一种色谱配体，其包含来自葡萄球菌蛋白A（SpA）的结构域C，或其功能片段或变体。色谱配体具有在现场（CIP）条件下耐受苛刻清洁的有利能力，并且能够结合抗体的Fab片段。配体可以具有末端偶联基团，例如精氨酸或半胱氨酸，以促进其与不溶性载体例如珠粒或膜的偶联。本发明还涉及分离抗体中使用配体的方法，以及涉及包括洗涤步骤和/或用碱再生的纯化方案。

8. 发明申请

WO2018122246A1 A METHOD AND SYSTEM FOR SEPARATING BIOMOLECULES 审中-公开
公开(公告)号：WO2018122246A1
公开(公告)日：2018-07-05
申请号：PCT/EP2017/084620
申请日：2017-12-27
申请人： GE HEALTHCARE BIO-SCIENCES AB
发明人： LIND, Ola , AVALLIN, Johan , LUNDIN, Andreas , NORRMAN, Nils , PALMGREN, Ronnie , RODRIGO, Gustav
IPC分类号： G01N33/543 , C12M1/00
摘要： A separation system, a method in a separation system and an elution arrangement to be provided in a separation system for separating a biomolecule from a cell culture are provided. The method comprises the steps of: - providing a feed from a cell culture (3; 103; 203) comprising said biomolecule to a magnetic separator (5; 105; 205) and providing to the magnetic separator magnetic beads comprising ligands capable of binding this biomolecule; - separating by the magnetic separator said magnetic beads with bound biomolecules from the rest of the feed; - forwarding said magnetic beads as a slurry with an added buffer to an elution cell (7; 107; 207); - eluting the bound biomolecules in the elution cell.

9. 发明申请

WO2014077762A1 MULTIMODAL ANION EXCHANGE MATRICES 审中-公开
标题翻译：多模式阴离子交换矩阵
公开(公告)号：WO2014077762A1
公开(公告)日：2014-05-22
申请号：PCT/SE2013/051325
申请日：2013-11-11
申请人： GE HEALTHCARE BIO-SCIENCES AB
发明人： MALOISEL, Jean-Luc , RODRIGO, Gustav , NORÉN, Bjorn , KUMBHAR, Virendra
IPC分类号： B01J20/26 , B01D15/08 , B01J20/285 , B01J20/289 , G01N30/02 , G01N33/544 , G01N33/548
CPC分类号： C07K1/165 , B01D15/3847 , B01D15/3885 , B01J20/265 , B01J20/28016 , B01J20/289 , B01J20/3204 , B01J20/3208 , B01J20/3219 , B01J20/3251 , B01J20/3253 , B01J20/3255 , B01J20/3285 , B01J41/20 , B01J2220/80 , C07K1/36 , G01N33/544 , B01D15/363 , B01D15/327
摘要： The invention discloses a separation matrix which comprises a plurality of separation ligands, defined by the formula R 1 - L 1 – N (R 3 ) – L 2 –R, immobilized on a support, wherein R 1 is a five-or six-membered, substituted or non-substituted ring structure or a hydroxyethyl or hydroxypropyl group; L 1 is either a methylene group or a covalent bond; R 2 is a five-or six-membered, substituted or non-substituted ring structure; L 2 is either a methylene group or a covalent bond;R 3 is a methyl group; and wherein if R 1 is a hydroxyethyl group and L 1 is a covalent bond, R 2 is a substituted aromatic ring structure or a substituted or non-substituted aliphatic ring structure.
摘要翻译：本发明公开了一种分离基质，其包含固定在载体上的式R1-L1-N（R3）-L2-R定义的多个分离配体，其中R1是五或六元的取代或非取代的取代的环结构或羟乙基或羟丙基; L1是亚甲基或共价键; R2是五或六元，取代或未取代的环结构; L2是亚甲基或共价键; R3是甲基; 并且其中如果R 1是羟乙基并且L 1是共价键，则R 2是取代的芳环结构或取代或未取代的脂族环结构。

10. 发明申请

WO2015005862A1 MUTATED IMMUNOGLOBULIN-BINDING POLYPEPTIDES 审中-公开
标题翻译：突变型免疫抑制蛋白结合多肽
公开(公告)号：WO2015005862A1
公开(公告)日：2015-01-15
申请号：PCT/SE2014/050882
申请日：2014-07-10
申请人： GE HEALTHCARE BIO-SCIENCES AB
发明人： RODRIGO, Gustav , ANDER, Mats , BAURÉN, Göran , BJÖRKMAN, Tomas
IPC分类号： C07K14/31 , B01D15/38 , C07K1/22 , C12N15/00
CPC分类号： C07K14/31 , B01D15/3809 , B01J20/286 , B01J20/3204 , B01J20/3212 , B01J20/3219 , B01J20/3274 , C07K1/22 , C07K14/745 , C07K16/00
摘要： The invention discloses a polypeptide with improved alkaline stability, which polypeptide comprises a mutant of a B or C domain of Staphylococcus Protein A (SpA), as specified by SEQ ID NO 1 or SEQ ID NO 2, or of Protein Z, as specified by SEQ ID NO 3, wherein at least the glutamic acid residue at position 15 has been mutated to an amino acid other than asparagine. The invention also discloses multimers of said polypeptide, as well as separation matrices comprising the multimers or polypeptides.
摘要翻译：本发明公开了一种具有改善的碱稳定性的多肽，该多肽包含如SEQ ID NO：1或SEQ ID NO：2或蛋白Z所定义的葡萄球菌蛋白A（SpA）的B或C结构域的突变体，如 SEQ ID NO 3，其中至少15位的谷氨酸残基已被突变成除了天冬酰胺之外的氨基酸。本发明还公开了所述多肽的多聚体，以及包含多聚体或多肽的分离基质。

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