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1. 发明申请

WO2009153348A3 DEVICE FOR MULTIPARAMETRIC ASSAYS 审中-公开
公开(公告)号：WO2009153348A3
公开(公告)日：2009-12-23
申请号：PCT/EP2009/057694
申请日：2009-06-19
申请人： EPPENDORF ARRAY TECHNOLOGIES S.A. , VAN HUFFEL, Christophe , LIEDECKER, Jens , HAMELS, Sandrine , DE LONGUEVILLE, Françoise
发明人： VAN HUFFEL, Christophe , LIEDECKER, Jens , HAMELS, Sandrine , DE LONGUEVILLE, Françoise
IPC分类号： B01L3/00
摘要： An assay device (C) compatible with multi-well format for performing a biological reaction between capture and target molecules, comprising: a cap (A) comprising side walls (7), and top surface (5) and a bottom surface (6), wherein the top surface (5) and bottom surface (6) are connected by at least one inlet channel (10) and at least one outlet channel (11) and wherein the top surface (5) of the cap (A) comprises a stopper indentation (15), a well (B) comprising side walls (3) and a bottom surface (4) upon which capture molecules are immobilized, wherein the cap (A) and the well (B) are made of different polymeric materials being preferably cycloolefin polymer for the cap (A) and polypropylene for the well (B), - wherein the side walls (7) of the cap (A) and the side walls (3) of the well (B) comprises complementary screwing means (12,13) to seal the cap (A) inside the well (B) to form a chamber (1) between the bottom surface (6) of the cap (A) and the bottom surface (4) of the well (B) and wherein the two said surfaces are essentially parallel to each other with a variation of distance between the two surfaces lower than 100 μm, better lower than 20 μm and, wherein the chamber (1) has a height of less than 5 mm, preferably less than 2 mm and which is vapour and fluid tight, when the cap (A) is sealed inside the well (B).

2. 发明申请

WO2006135992A1 METHOD AND MEANS FOR DETECTING AND/OR QUANTIFYING HIERARCHICAL MOLECULAR CHANGE OF A CELL IN RESPONSE TO AN EXTERNAL STIMULUS 审中-公开
标题翻译：用于检测和/或定量分析细胞在外部刺激反应中的分子变化的方法和手段
公开(公告)号：WO2006135992A1
公开(公告)日：2006-12-28
申请号：PCT/BE2006/000071
申请日：2006-06-23
申请人： EPPENDORF ARRAY TECHNOLOGIES S.A. , REMACLE, José , BERTHOLET, Vincent , MARGAINE, Sylvain , DELONGUEVILLE, Françoise , VAN HUFFEL, Christophe , MAINFROID, Véronique , PLENNEVAUX, Christelle
发明人： REMACLE, José , BERTHOLET, Vincent , MARGAINE, Sylvain , DELONGUEVILLE, Françoise , VAN HUFFEL, Christophe , MAINFROID, Véronique , PLENNEVAUX, Christelle
IPC分类号： G01N33/68 , C12Q1/48 , G01N33/543 , C12Q1/68
CPC分类号： C12Q1/485 , G01N33/543 , G01N33/6875
摘要： The present invention is related to a method for detecting and/or quantifying hierarchical (regulated) molecular changes of a cell in response to any external stimulus.
摘要翻译：本发明涉及用于响应于任何外部刺激来检测和/或量化细胞的分级（调节）分子变化的方法。

3. 发明申请

WO2006108473A1 METHOD FOR THE DETERMINATION OF CELLULAR TRANSCRIPTIONAL REGULATION BY MICRO RNAS 审中-公开
标题翻译：用于通过微RNA测定细胞转录调控的方法
公开(公告)号：WO2006108473A1
公开(公告)日：2006-10-19
申请号：PCT/EP2006/002140
申请日：2006-03-08
申请人： EPPENDORF ARRAY TECHNOLOGIES , VAN HUFFEL, Christophe , REMACLE José , BÜLOW, Sven , ZAMMATTEO, Nathalie
发明人： VAN HUFFEL, Christophe , REMACLE José , BÜLOW, Sven , ZAMMATTEO, Nathalie
IPC分类号： C12Q1/68 , C12N15/11
CPC分类号： C12Q1/6837 , C12N15/63 , C12Q2565/515
摘要： The present invention relates to a new method for determining the RNAi mediated transcriptional regulation of a cell by the determination of a pattern of at least 3 miRNA detected simultaneously and quantified in the same cell extract. The determination of a pattern of miRNA comprises the steps of: (i) providing an array onto which are fixed capture probes, said capture probes being arranged on pre-determined locations and reflecting the genomic or transcriptional matter of a cell; (ii) isolating a miRNA pool potentially present from a cell; (iii) elongating or ligating said miRNAs into labeled capture probes, (iv) contacting said labeled polynucleotides with the array under conditions allowing hybridization of the labeled polynucleotides to complementary capture probes present on the array; (v) detecting and quantifying a signal present on the specific locations of the array, wherein the detection of a pattern of at least 3 signals on the array reflects the pattern of miRNAs being involved in the RNAi mediated cellular transcriptional regulation.
摘要翻译：本发明涉及通过测定在同一细胞提取物中同时检测并定量检测到的至少3种miRNA的模式来确定细胞RNAi介导的转录调控的新方法。 miRNA的模式的确定包括以下步骤：（i）提供阵列，固定的捕获探针在所述阵列上，所述捕获探针布置在预定位置上并反映细胞的基因组或转录物质; （ii）分离潜在地存在于细胞中的miRNA池; （iii）将所述miRNA延伸或连接到标记的捕获探针中，（iv）在允许标记的多核苷酸与阵列上存在的互补捕获探针杂交的条件下将所述标记的多核苷酸与阵列接触; （v）检测和量化存在于阵列的特定位置上的信号，其中对阵列上的至少3个信号的模式的检测反映参与RNAi介导的细胞转录调节的miRNA模式。

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