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1. 发明申请

WO2009153348A3 DEVICE FOR MULTIPARAMETRIC ASSAYS 审中-公开
公开(公告)号：WO2009153348A3
公开(公告)日：2009-12-23
申请号：PCT/EP2009/057694
申请日：2009-06-19
申请人： EPPENDORF ARRAY TECHNOLOGIES S.A. , VAN HUFFEL, Christophe , LIEDECKER, Jens , HAMELS, Sandrine , DE LONGUEVILLE, Françoise
发明人： VAN HUFFEL, Christophe , LIEDECKER, Jens , HAMELS, Sandrine , DE LONGUEVILLE, Françoise
IPC分类号： B01L3/00
摘要： An assay device (C) compatible with multi-well format for performing a biological reaction between capture and target molecules, comprising: a cap (A) comprising side walls (7), and top surface (5) and a bottom surface (6), wherein the top surface (5) and bottom surface (6) are connected by at least one inlet channel (10) and at least one outlet channel (11) and wherein the top surface (5) of the cap (A) comprises a stopper indentation (15), a well (B) comprising side walls (3) and a bottom surface (4) upon which capture molecules are immobilized, wherein the cap (A) and the well (B) are made of different polymeric materials being preferably cycloolefin polymer for the cap (A) and polypropylene for the well (B), - wherein the side walls (7) of the cap (A) and the side walls (3) of the well (B) comprises complementary screwing means (12,13) to seal the cap (A) inside the well (B) to form a chamber (1) between the bottom surface (6) of the cap (A) and the bottom surface (4) of the well (B) and wherein the two said surfaces are essentially parallel to each other with a variation of distance between the two surfaces lower than 100 μm, better lower than 20 μm and, wherein the chamber (1) has a height of less than 5 mm, preferably less than 2 mm and which is vapour and fluid tight, when the cap (A) is sealed inside the well (B).

2. 发明申请

WO2008034896A8 IDENTIFICATION AND QUANTIFICATION OF A PLURALITY OF BIOLOGICAL (MICRO)ORGANISMS OR THEIR COMPONENTS 审中-公开
公开(公告)号：WO2008034896A8
公开(公告)日：2008-03-27
申请号：PCT/EP2007/060037
申请日：2007-09-21
申请人： EPPENDORF ARRAY TECHNOLOGIES S.A. , ALEXANDRE, Isabelle , HUSAR, Dieter , ZAMMATTEO, Nathalie , KOEHN, Heinz , REMACLE, José , MARGAINE, Sylvain
发明人： ALEXANDRE, Isabelle , HUSAR, Dieter , ZAMMATTEO, Nathalie , KOEHN, Heinz , REMACLE, José , MARGAINE, Sylvain
IPC分类号： B01L3/00 , C12Q1/68
摘要： Disclosed is a system and method conducting real-time PCR. Capture molecules of a specific design are immobilized on a solid support, and contacted with amplicons produced in one or more PCR cycles. Detection of amplicons may take place during or between the PCR cycles while the solid support is in fluidic contact with the PCR solution. In one embodiment detection of the amplicons takes place when the solid support is not in fluidic contact with the PCR solution. In another embodiment, labeled amplicons are detected using an evanescent field while hybridized to capture probes immobilized on a solid support in the Real-time PCR reaction chamber. The method is suitable for the simultaneous detection and quantification of closely homologous target molecules.

3. 发明申请

WO2006053770A8 REAL-TIME PCR OF TARGETS ON A MICRO-ARRAY 审中-公开
标题翻译：微阵列上的目标的实时PCR
公开(公告)号：WO2006053770A8
公开(公告)日：2006-08-24
申请号：PCT/EP2005012383
申请日：2005-11-18
申请人： EPPENDORF ARRAY TECHNOLOGIES , REMACLE JOSE , ALEXANDRE ISABELLE , MARGAINE SYLVAIN , HUSAR DIETER
发明人： REMACLE JOSE , ALEXANDRE ISABELLE , MARGAINE SYLVAIN , HUSAR DIETER
IPC分类号： C12Q1/68 , B01L3/00
CPC分类号： G01N21/6452 , B01L7/52 , B01L2300/0636 , B01L2300/0822 , C12Q1/6837 , G01N21/6428 , C12Q2561/113
摘要： The present invention relates to a method and apparatus for monitoring on a micro-array a PCR amplification of a nucleotide molecule being present in a solution. The method comprises the steps of: providing a support (15) having fixed upon its surface a microarray comprising at least a capture molecule (20) being immobilized in specifically localized areas (21) of said support and a reaction chamber (14) on; introducing a solution containing said nucleotide molecule into said reaction chamber (14) and reagents for nucleotide molecule amplification and labelling; submitted the solution to at least 2 thermal cycles having at least 2 and preferably 3 different temperature steps in order to obtain labelled target nucleotide molecule (13) by PCR amplification; performing at least a measurement of the labelled target nucleotide molecule in at least one thermal cycle by incubating said labelled target nucleotide molecule (13) under conditions allowing a specific binding between said target nucleotide molecule (13) and its corresponding capture molecule (20) and measuring the light emission (7) from the bound labelled target nucleotide molecule in response to excitation light (2) with said solution being present in the chamber and containing the labelled target nucleotide molecule (13), wherein the surface of emission for a localized area is comprised between about 0.1 µm 2 and about 75 mm 2 , and; Processing the data obtained in at least one thermal cycle in order to detect and/or quantify the amount of nucleotide molecule present in the solution before the amplification.
摘要翻译：本发明涉及用于在微阵列上监测存在于溶液中的核苷酸分子的PCR扩增的方法和设备。所述方法包括以下步骤：提供载体（15），所述载体（15）在其表面上固定有微阵列，所述微阵列包含固定在所述载体的特定局部区域（21）中的捕获分子（20）和反应室（14）将含有所述核苷酸分子的溶液引入所述反应室（14）和用于核苷酸分子扩增和标记的试剂; 将所述溶液提交至少2个具有至少2个，优选3个不同温度步骤的热循环以通过PCR扩增获得标记的靶核苷酸分子（13）; 在允许所述靶核苷酸分子（13）与其相应的捕获分子（20）之间的特异性结合的条件下，通过温育所述标记的靶核苷酸分子（13），在至少一个热循环中执行至少一个标记的靶核苷酸分子的测量; 响应于激发光（2）测量来自结合的经标记的靶核苷酸分子的光发射（7），所述溶液存在于所述室中且含有经标记的靶核苷酸分子（13），其中局部区域包含在约0.1μm2至约75mm 2，^{和; 处理在至少一个热循环中获得的数据，以便在扩增前检测和/或定量溶液中存在的核苷酸分子的量。}

4. 发明申请

WO2006135992A1 METHOD AND MEANS FOR DETECTING AND/OR QUANTIFYING HIERARCHICAL MOLECULAR CHANGE OF A CELL IN RESPONSE TO AN EXTERNAL STIMULUS 审中-公开
标题翻译：用于检测和/或定量分析细胞在外部刺激反应中的分子变化的方法和手段
公开(公告)号：WO2006135992A1
公开(公告)日：2006-12-28
申请号：PCT/BE2006/000071
申请日：2006-06-23
申请人： EPPENDORF ARRAY TECHNOLOGIES S.A. , REMACLE, José , BERTHOLET, Vincent , MARGAINE, Sylvain , DELONGUEVILLE, Françoise , VAN HUFFEL, Christophe , MAINFROID, Véronique , PLENNEVAUX, Christelle
发明人： REMACLE, José , BERTHOLET, Vincent , MARGAINE, Sylvain , DELONGUEVILLE, Françoise , VAN HUFFEL, Christophe , MAINFROID, Véronique , PLENNEVAUX, Christelle
IPC分类号： G01N33/68 , C12Q1/48 , G01N33/543 , C12Q1/68
CPC分类号： C12Q1/485 , G01N33/543 , G01N33/6875
摘要： The present invention is related to a method for detecting and/or quantifying hierarchical (regulated) molecular changes of a cell in response to any external stimulus.
摘要翻译：本发明涉及用于响应于任何外部刺激来检测和/或量化细胞的分级（调节）分子变化的方法。

5. 发明申请

WO2006086859A2 METHOD AND KIT TO PROFILE TUMOURS BY BIOMARKER ANALYSES INCLUDING TRANSCRIPTIONAL FACTOR ASSAYS 审中-公开
标题翻译：包括转录因子测定在内的生物识别分析的方法和工具包
公开(公告)号：WO2006086859A2
公开(公告)日：2006-08-24
申请号：PCT/BE2006/000012
申请日：2006-02-16
申请人： EPPENDORF ARRAY TECHNOLOGIES S.A. , MAINFROID, Véronique , REMACLE, José
发明人： MAINFROID, Véronique , REMACLE, José
IPC分类号： C12Q1/68 , B01L3/00 , G01N33/574
CPC分类号： G01N33/574
摘要： The present invention is related to a method and kit (or device) for the detection and/or the quantification of biomarkers related to tumourigenesis. Said method is advantageously used to propose or adapt an anti-tumoural therapeutic protocol to be administered to a subject. Furthermore, said method and kit (or device) are a technical platform for the identification of new compounds, which are preferably used at (a) specific step(s) of an anti-tumoural therapeutic protocol.
摘要翻译：本发明涉及用于检测和/或定量与肿瘤发生相关的生物标志物的方法和试剂盒（或装置）。所述方法有利地用于提出或适应施用于受试者的抗肿瘤治疗方案。此外，所述方法和试剂盒（或装置）是用于鉴定新化合物的技术平台，其优选用于（a）抗肿瘤治疗方案的具体步骤。

6. 发明申请

WO2006053770A1 REAL-TIME PCR OF TARGETS ON A MICRO-ARRAY 审中-公开
标题翻译：目标在微阵列上的实时PCR
公开(公告)号：WO2006053770A1
公开(公告)日：2006-05-26
申请号：PCT/EP2005/012383
申请日：2005-11-18
申请人： EPPENDORF ARRAY TECHNOLOGIES , REMACLE, José , ALEXANDRE, Isabelle , MARGAINE, Sylvain , HUSAR, Dieter
发明人： REMACLE, José , ALEXANDRE, Isabelle , MARGAINE, Sylvain , HUSAR, Dieter
IPC分类号： C12Q1/68 , B01L3/00
CPC分类号： G01N21/6452 , B01L7/52 , B01L2300/0636 , B01L2300/0822 , C12Q1/6837 , G01N21/6428 , C12Q2561/113
摘要： The present invention relates to a method and apparatus for monitoring on a micro-array a PCR amplification of a nucleotide molecule being present in a solution. The method comprises the steps of: providing a support (15) having fixed upon its surface a microarray comprising at least a capture molecule (20) being immobilized in specifically localized areas (21) of said support and a reaction chamber (14) on; introducing a solution containing said nucleotide molecule into said reaction chamber (14) and reagents for nucleotide molecule amplification and labelling; submitted the solution to at least 2 thermal cycles having at least 2 and preferably 3 different temperature steps in order to obtain labelled target nucleotide molecule (13) by PCR amplification; performing at least a measurement of the labelled target nucleotide molecule in at least one thermal cycle by incubating said labelled target nucleotide molecule (13) under conditions allowing a specific binding between said target nucleotide molecule (13) and its corresponding capture molecule (20) and measuring the light emission (7) from the bound labelled target nucleotide molecule in response to excitation light (2) with said solution being present in the chamber and containing the labelled target nucleotide molecule (13), wherein the surface of emission for a localized area is comprised between about 0.1 µm 2 and about 75 mm 2 , and; Processing the data obtained in at least one thermal cycle in order to detect and/or quantify the amount of nucleotide molecule present in the solution before the amplification.
摘要翻译：本发明涉及用于在微阵列上监测存在于溶液中的核苷酸分子的PCR扩增的方法和装置。该方法包括以下步骤：提供在其表面上具有固定的支架（15），所述微阵列至少包括固定在所述支撑体的特定局部区域（21）中的捕获分子（20）和反应室（14）; 将含有所述核苷酸分子的溶液引入所述反应室（14）和用于核苷酸分子扩增和标记的试剂; 将溶液提交至少2个具有至少2个并且优选3个不同温度步骤的热循环，以通过PCR扩增获得标记的靶核苷酸分子（13）; 通过在允许所述靶核苷酸分子（13）和其相应的捕获分子（20）之间的特异性结合的条件下温育所述标记的靶核苷酸分子（13）至少在一个热循环中进行标记的靶核苷酸分子的至少一个测量，以及响应于激发光（2）测量来自结合的标记的靶核苷酸分子的光发射（7），所述溶液存在于所述室中并含有标记的靶核苷酸分子（13），其中局部区域的发射表面包括约0.1μm2和约75mm 2之间，以及; 处理在至少一个热循环中获得的数据，以便检测和/或定量扩增前溶液中存在的核苷酸分子的量。

7. 发明申请

WO2006053769A1 REAL-TIME QUANTIFICATION OF MULTIPLE TARGETS ON A MICRO-ARRAY 审中-公开
标题翻译：在微阵列上实现多目标定量
公开(公告)号：WO2006053769A1
公开(公告)日：2006-05-26
申请号：PCT/EP2005/012382
申请日：2005-11-18
申请人： EPPENDORF ARRAY TECHNOLOGIES , REMACLE, José , ALEXANDRE, Isabelle , MARGAINE, Sylvain , HUSAR, Dieter , ZAMMATTEO, Nathalie , KOEHN, Heinz
发明人： REMACLE, José , ALEXANDRE, Isabelle , MARGAINE, Sylvain , HUSAR, Dieter , ZAMMATTEO, Nathalie , KOEHN, Heinz
IPC分类号： C12Q1/68
CPC分类号： G01N21/6452 , B01L7/52 , B01L2300/0636 , B01L2300/0822 , C12Q1/6837 , G01N21/6428 , C12Q2561/113
摘要： The present invention relates to a method and apparatus for monitoring a real-time quantification of multiple target molecules during their binding on capture molecules of a micro-array. The method comprises the steps of: placing, in a chamber (14), a support (15) having fixed upon its surface a micro-array comprising at least 5 capture molecules (20) being immobilized in specifically localized areas (21) of said support; introducing said labeled target molecules solution (13) into the chamber; incubating said labeled target molecules under stable and controlled temperature conditions to allow the binding between said target and capture molecules; directing an excitation light (2) from a light source (1) on the surface of the micro-array; measuring the electromagnetic light emission (7) from the bound target molecules in response to said excitation light in presence of the solution containing the target molecules wherein the surface of emission for a localized area is comprised between about 0.1 µm 2 and about 10mm2 and wherein each of the at least 4 localized areas is monitored with time with at least two measurements being done per localized area (21), and processing and storing the values of different measurements and quantifying at least 4 different target molecules present in solution using at least one measurement value for each said target.
摘要翻译：本发明涉及用于监测多个靶分子在其结合微阵列的捕获分子时的实时定量的方法和装置。该方法包括以下步骤：在腔室（14）中放置具有固定在其表面上的支撑体（15），该微阵列包括至少5个俘获分子（20），该俘获分子固定在所述支持; 将所述标记的靶分子溶液（13）引入所述室中; 在稳定和受控的温度条件下孵育所述标记的靶分子以允许所述靶和捕获分子之间的结合; 将来自光源（1）的激发光（2）引导到微阵列的表面上; 在含有靶分子的溶液的存在下，响应于所述激发光，从结合的靶分子测量电磁光发射（7），其中局部区域的发射表面在约0.1μm2 / >和约10mm 2，并且其中至少4个局部区域中的每一个随着时间监测，每个局部区域（21）进行至少两次测量，并且处理和存储不同测量值并定量存在至少4种不同的靶分子在使用每个所述目标的至少一个测量值的解决方案中。

8. 发明申请

WO2007132002A1 IDENTIFICATION AND QUANTIFICATION OF A PLURALITY OF BIOLOGICAL (MICRO)ORGANISMS OR THEIR COMPONENTS 审中-公开
标题翻译：生物多样性（微生物）或其组分的识别和定量
公开(公告)号：WO2007132002A1
公开(公告)日：2007-11-22
申请号：PCT/EP2007/054696
申请日：2007-05-15
申请人： EPPENDORF ARRAY TECHNOLOGIES S.A. , REMACLE, José , ALEXANDRE, Isabelle , KOEHN, Heinz , MARGAINE, Sylvian , HUSAR, Dieter , ZAMMATEO, Nathalie
发明人： REMACLE, José , ALEXANDRE, Isabelle , KOEHN, Heinz , MARGAINE, Sylvian , HUSAR, Dieter , ZAMMATEO, Nathalie
IPC分类号： C12Q1/68
CPC分类号： B01L3/50851 , B01L7/52 , B01L2300/0636 , B01L2300/0803 , B01L2300/0819 , C12Q1/686 , C12Q2561/113
摘要： Disclosed is a system and method conducting real-time PCR. Unlabeled capture molecules of a specific design are immobilized on a solid support, and contacted with amplicons produced in one or more PCR cycles. Detection of amplicons may take place during or between the PCR cycles while the solid support is in fluidic contact with the PCR solution. In an alternate embodiment detection of the amplicons takes place when the solid support is not in fluidic contact with the PCR solution. The method is suitable for the simultaneous detection and quantification of closely homologous target molecules.
摘要翻译：公开了进行实时PCR的系统和方法。将具体设计的未标记的捕获分子固定在固体支持物上，并与在一个或多个PCR循环中产生的扩增子接触。扩增子的检测可以在PCR循环期间或之间进行，而固体支持物与PCR溶液流体接触。在替代实施方案中，当固体支持物不与PCR溶液流体接触时，扩增子的检测发生。该方法适用于同时检测和定量分析近似同源的靶分子。

9. 发明申请

WO2006108473A1 METHOD FOR THE DETERMINATION OF CELLULAR TRANSCRIPTIONAL REGULATION BY MICRO RNAS 审中-公开
标题翻译：用于通过微RNA测定细胞转录调控的方法
公开(公告)号：WO2006108473A1
公开(公告)日：2006-10-19
申请号：PCT/EP2006/002140
申请日：2006-03-08
申请人： EPPENDORF ARRAY TECHNOLOGIES , VAN HUFFEL, Christophe , REMACLE José , BÜLOW, Sven , ZAMMATTEO, Nathalie
发明人： VAN HUFFEL, Christophe , REMACLE José , BÜLOW, Sven , ZAMMATTEO, Nathalie
IPC分类号： C12Q1/68 , C12N15/11
CPC分类号： C12Q1/6837 , C12N15/63 , C12Q2565/515
摘要： The present invention relates to a new method for determining the RNAi mediated transcriptional regulation of a cell by the determination of a pattern of at least 3 miRNA detected simultaneously and quantified in the same cell extract. The determination of a pattern of miRNA comprises the steps of: (i) providing an array onto which are fixed capture probes, said capture probes being arranged on pre-determined locations and reflecting the genomic or transcriptional matter of a cell; (ii) isolating a miRNA pool potentially present from a cell; (iii) elongating or ligating said miRNAs into labeled capture probes, (iv) contacting said labeled polynucleotides with the array under conditions allowing hybridization of the labeled polynucleotides to complementary capture probes present on the array; (v) detecting and quantifying a signal present on the specific locations of the array, wherein the detection of a pattern of at least 3 signals on the array reflects the pattern of miRNAs being involved in the RNAi mediated cellular transcriptional regulation.
摘要翻译：本发明涉及通过测定在同一细胞提取物中同时检测并定量检测到的至少3种miRNA的模式来确定细胞RNAi介导的转录调控的新方法。 miRNA的模式的确定包括以下步骤：（i）提供阵列，固定的捕获探针在所述阵列上，所述捕获探针布置在预定位置上并反映细胞的基因组或转录物质; （ii）分离潜在地存在于细胞中的miRNA池; （iii）将所述miRNA延伸或连接到标记的捕获探针中，（iv）在允许标记的多核苷酸与阵列上存在的互补捕获探针杂交的条件下将所述标记的多核苷酸与阵列接触; （v）检测和量化存在于阵列的特定位置上的信号，其中对阵列上的至少3个信号的模式的检测反映参与RNAi介导的细胞转录调节的miRNA模式。

10. 发明申请

WO2006066369A3 METHOD AND KIT FOR THE DETECTION OF A LARGE NUMBER OF GENES RELATED TO ANTIBIOTIC RESISTANCE IN MICROORGANISMS 审中-公开
公开(公告)号：WO2006066369A3
公开(公告)日：2006-06-29
申请号：PCT/BE2005/000191
申请日：2005-12-23
申请人： EPPENDORF ARRAY TECHNOLOGIES S.A. , REMACLE, José , HAMELS, Sandrine , BURTEAU, Sophie
发明人： REMACLE, José , HAMELS, Sandrine , BURTEAU, Sophie
IPC分类号： C12Q1/68
摘要： The present invention is related to a method and kit (or device) for identifying and/or quantifying in an array mRNA nucleotide sequences related to resistance to antibiotic(s) being expressed in a (micro)organism present in a sample.

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