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1. 发明申请

WO2006135992A1 METHOD AND MEANS FOR DETECTING AND/OR QUANTIFYING HIERARCHICAL MOLECULAR CHANGE OF A CELL IN RESPONSE TO AN EXTERNAL STIMULUS 审中-公开
标题翻译：用于检测和/或定量分析细胞在外部刺激反应中的分子变化的方法和手段
公开(公告)号：WO2006135992A1
公开(公告)日：2006-12-28
申请号：PCT/BE2006/000071
申请日：2006-06-23
申请人： EPPENDORF ARRAY TECHNOLOGIES S.A. , REMACLE, José , BERTHOLET, Vincent , MARGAINE, Sylvain , DELONGUEVILLE, Françoise , VAN HUFFEL, Christophe , MAINFROID, Véronique , PLENNEVAUX, Christelle
发明人： REMACLE, José , BERTHOLET, Vincent , MARGAINE, Sylvain , DELONGUEVILLE, Françoise , VAN HUFFEL, Christophe , MAINFROID, Véronique , PLENNEVAUX, Christelle
IPC分类号： G01N33/68 , C12Q1/48 , G01N33/543 , C12Q1/68
CPC分类号： C12Q1/485 , G01N33/543 , G01N33/6875
摘要： The present invention is related to a method for detecting and/or quantifying hierarchical (regulated) molecular changes of a cell in response to any external stimulus.
摘要翻译：本发明涉及用于响应于任何外部刺激来检测和/或量化细胞的分级（调节）分子变化的方法。

2. 发明申请

WO2006086859A2 METHOD AND KIT TO PROFILE TUMOURS BY BIOMARKER ANALYSES INCLUDING TRANSCRIPTIONAL FACTOR ASSAYS 审中-公开
标题翻译：包括转录因子测定在内的生物识别分析的方法和工具包
公开(公告)号：WO2006086859A2
公开(公告)日：2006-08-24
申请号：PCT/BE2006/000012
申请日：2006-02-16
申请人： EPPENDORF ARRAY TECHNOLOGIES S.A. , MAINFROID, Véronique , REMACLE, José
发明人： MAINFROID, Véronique , REMACLE, José
IPC分类号： C12Q1/68 , B01L3/00 , G01N33/574
CPC分类号： G01N33/574
摘要： The present invention is related to a method and kit (or device) for the detection and/or the quantification of biomarkers related to tumourigenesis. Said method is advantageously used to propose or adapt an anti-tumoural therapeutic protocol to be administered to a subject. Furthermore, said method and kit (or device) are a technical platform for the identification of new compounds, which are preferably used at (a) specific step(s) of an anti-tumoural therapeutic protocol.
摘要翻译：本发明涉及用于检测和/或定量与肿瘤发生相关的生物标志物的方法和试剂盒（或装置）。所述方法有利地用于提出或适应施用于受试者的抗肿瘤治疗方案。此外，所述方法和试剂盒（或装置）是用于鉴定新化合物的技术平台，其优选用于（a）抗肿瘤治疗方案的具体步骤。

3. 发明申请

WO2008028683A1 INSULATED SUBSTRATE IMPEDANCE TRANSDUCERS 审中-公开
标题翻译：绝缘衬底阻抗传导器
公开(公告)号：WO2008028683A1
公开(公告)日：2008-03-13
申请号：PCT/EP2007/007861
申请日：2007-09-10
申请人： UNIVERSITE CATHOLIQUE DE LOUVAIN , FACULTES UNIVERSITAIRES NOTRE-DAME DE LA PAIX , PAMPIN, Rémi, Sébastien , FLANDRE, Denis , MORENO-HAGELSIEB, Luis , FOULTIER, Boris , REMACLE, José
发明人： PAMPIN, Rémi, Sébastien , FLANDRE, Denis , MORENO-HAGELSIEB, Luis , FOULTIER, Boris , REMACLE, José
IPC分类号： G01N27/414 , G01N27/12
CPC分类号： G01N27/414 , C12Q1/6816 , C12Q1/6825 , G01N33/48728 , C12Q2565/607
摘要： The present invention provides an electronic transducer (10) and a method for detecting and/or characterising target materials or physico-chemical stimuli in an external medium (8) using the electronic transducer (10). The electronic transducer (10) comprises a sensing element (3) featuring a variable conductance when exposed to a stimulus from the external medium and a first and second electrode (5a, 5b) spaced apart on or in a sensing material surface of a substrate, the sensing element being provided in or on he substrate and being located between the first and the second electrodes (5a, 5b) forming a pair of sensing electrodes for sensing a change in conductance of the sensing element (3) in a direction substantially parallel to the sensing material surface, at least one of the sensing electrodes (5a, 5b) being electrically insulated from the sensing element (3) by a dielectric layer (4), so as to be capacitively coupled to the sensing element (3). An insulating layer or target specific layer (7) may optionally be provided covering the sensing element (3) and optionally the sensing electrodes and being adapted for contact with the external medium (8). Electrical measurements made between the pair of sensing electrodes (5a, 5b) are influenced by the impedance of the channel (3) which is affected by the presence of the medium (8) to be tested.
摘要翻译：本发明提供一种电子换能器（10）和用于使用电子换能器（10）检测和/或表征外部介质（8）中的目标材料或物理 - 化学刺激的方法。电子换能器（10）包括感测元件（3），其在暴露于来自外部介质的刺激时具有可变电导，以及在衬底的感测材料表面上或其中的间隔开的第一和第二电极（5a，5b）所述感测元件设置在所述衬底中或衬底上并且位于所述第一和第二电极（5a，5b）之间，形成一对感测电极，用于感测感测元件（3）在基本上平行于感测材料表面，至少一个感测电极（5a，5b）通过电介质层（4）与感测元件（3）电绝缘，以便电容耦合到感测元件（3）。可以可选地设置覆盖感测元件（3）和可选地感测电极并适于与外部介质（8）接触的绝缘层或靶特定层（7）。在一对感测电极（5a，5b）之间进行的电测量受到待测试介质（8）的存在影响的通道（3）的阻抗的影响。

4. 发明申请

WO2006053770A1 REAL-TIME PCR OF TARGETS ON A MICRO-ARRAY 审中-公开
标题翻译：目标在微阵列上的实时PCR
公开(公告)号：WO2006053770A1
公开(公告)日：2006-05-26
申请号：PCT/EP2005/012383
申请日：2005-11-18
申请人： EPPENDORF ARRAY TECHNOLOGIES , REMACLE, José , ALEXANDRE, Isabelle , MARGAINE, Sylvain , HUSAR, Dieter
发明人： REMACLE, José , ALEXANDRE, Isabelle , MARGAINE, Sylvain , HUSAR, Dieter
IPC分类号： C12Q1/68 , B01L3/00
CPC分类号： G01N21/6452 , B01L7/52 , B01L2300/0636 , B01L2300/0822 , C12Q1/6837 , G01N21/6428 , C12Q2561/113
摘要： The present invention relates to a method and apparatus for monitoring on a micro-array a PCR amplification of a nucleotide molecule being present in a solution. The method comprises the steps of: providing a support (15) having fixed upon its surface a microarray comprising at least a capture molecule (20) being immobilized in specifically localized areas (21) of said support and a reaction chamber (14) on; introducing a solution containing said nucleotide molecule into said reaction chamber (14) and reagents for nucleotide molecule amplification and labelling; submitted the solution to at least 2 thermal cycles having at least 2 and preferably 3 different temperature steps in order to obtain labelled target nucleotide molecule (13) by PCR amplification; performing at least a measurement of the labelled target nucleotide molecule in at least one thermal cycle by incubating said labelled target nucleotide molecule (13) under conditions allowing a specific binding between said target nucleotide molecule (13) and its corresponding capture molecule (20) and measuring the light emission (7) from the bound labelled target nucleotide molecule in response to excitation light (2) with said solution being present in the chamber and containing the labelled target nucleotide molecule (13), wherein the surface of emission for a localized area is comprised between about 0.1 µm 2 and about 75 mm 2 , and; Processing the data obtained in at least one thermal cycle in order to detect and/or quantify the amount of nucleotide molecule present in the solution before the amplification.
摘要翻译：本发明涉及用于在微阵列上监测存在于溶液中的核苷酸分子的PCR扩增的方法和装置。该方法包括以下步骤：提供在其表面上具有固定的支架（15），所述微阵列至少包括固定在所述支撑体的特定局部区域（21）中的捕获分子（20）和反应室（14）; 将含有所述核苷酸分子的溶液引入所述反应室（14）和用于核苷酸分子扩增和标记的试剂; 将溶液提交至少2个具有至少2个并且优选3个不同温度步骤的热循环，以通过PCR扩增获得标记的靶核苷酸分子（13）; 通过在允许所述靶核苷酸分子（13）和其相应的捕获分子（20）之间的特异性结合的条件下温育所述标记的靶核苷酸分子（13）至少在一个热循环中进行标记的靶核苷酸分子的至少一个测量，以及响应于激发光（2）测量来自结合的标记的靶核苷酸分子的光发射（7），所述溶液存在于所述室中并含有标记的靶核苷酸分子（13），其中局部区域的发射表面包括约0.1μm2和约75mm 2之间，以及; 处理在至少一个热循环中获得的数据，以便检测和/或定量扩增前溶液中存在的核苷酸分子的量。

5. 发明申请

WO2006053769A1 REAL-TIME QUANTIFICATION OF MULTIPLE TARGETS ON A MICRO-ARRAY 审中-公开
标题翻译：在微阵列上实现多目标定量
公开(公告)号：WO2006053769A1
公开(公告)日：2006-05-26
申请号：PCT/EP2005/012382
申请日：2005-11-18
申请人： EPPENDORF ARRAY TECHNOLOGIES , REMACLE, José , ALEXANDRE, Isabelle , MARGAINE, Sylvain , HUSAR, Dieter , ZAMMATTEO, Nathalie , KOEHN, Heinz
发明人： REMACLE, José , ALEXANDRE, Isabelle , MARGAINE, Sylvain , HUSAR, Dieter , ZAMMATTEO, Nathalie , KOEHN, Heinz
IPC分类号： C12Q1/68
CPC分类号： G01N21/6452 , B01L7/52 , B01L2300/0636 , B01L2300/0822 , C12Q1/6837 , G01N21/6428 , C12Q2561/113
摘要： The present invention relates to a method and apparatus for monitoring a real-time quantification of multiple target molecules during their binding on capture molecules of a micro-array. The method comprises the steps of: placing, in a chamber (14), a support (15) having fixed upon its surface a micro-array comprising at least 5 capture molecules (20) being immobilized in specifically localized areas (21) of said support; introducing said labeled target molecules solution (13) into the chamber; incubating said labeled target molecules under stable and controlled temperature conditions to allow the binding between said target and capture molecules; directing an excitation light (2) from a light source (1) on the surface of the micro-array; measuring the electromagnetic light emission (7) from the bound target molecules in response to said excitation light in presence of the solution containing the target molecules wherein the surface of emission for a localized area is comprised between about 0.1 µm 2 and about 10mm2 and wherein each of the at least 4 localized areas is monitored with time with at least two measurements being done per localized area (21), and processing and storing the values of different measurements and quantifying at least 4 different target molecules present in solution using at least one measurement value for each said target.
摘要翻译：本发明涉及用于监测多个靶分子在其结合微阵列的捕获分子时的实时定量的方法和装置。该方法包括以下步骤：在腔室（14）中放置具有固定在其表面上的支撑体（15），该微阵列包括至少5个俘获分子（20），该俘获分子固定在所述支持; 将所述标记的靶分子溶液（13）引入所述室中; 在稳定和受控的温度条件下孵育所述标记的靶分子以允许所述靶和捕获分子之间的结合; 将来自光源（1）的激发光（2）引导到微阵列的表面上; 在含有靶分子的溶液的存在下，响应于所述激发光，从结合的靶分子测量电磁光发射（7），其中局部区域的发射表面在约0.1μm2 / >和约10mm 2，并且其中至少4个局部区域中的每一个随着时间监测，每个局部区域（21）进行至少两次测量，并且处理和存储不同测量值并定量存在至少4种不同的靶分子在使用每个所述目标的至少一个测量值的解决方案中。

6. 发明申请

WO2007132001A1 DESIGN OF CAPTURE MOLECULES FOR THE DETECTION OF AMPLICONS WITH HIGH SENSITIVITY 审中-公开
标题翻译：用于检测高灵敏度的AMPLICON的捕获分子的设计
公开(公告)号：WO2007132001A1
公开(公告)日：2007-11-22
申请号：PCT/EP2007/054692
申请日：2007-05-15
申请人： EPPENDORF ARRAY TECHNOLOGIES S.A. , REMACLE, José , HAMELS, Sandrine
发明人： REMACLE, José , HAMELS, Sandrine
IPC分类号： C12Q1/68 , B01J19/00
CPC分类号： C12Q1/6837 , B01J2219/00529 , B01J2219/00608 , B01J2219/0061 , B01J2219/00612 , B01J2219/00626 , B01J2219/00637 , B01J2219/00659 , B01J2219/00722 , C12Q1/6832 , C12Q1/6848 , C12Q2525/197
摘要： The invention relates to a method for the design and/or the preparation of a polynucleotide capture molecule for detecting an amplicon having one strand serving as target to be detected and/or quantified after hybridization on said capture molecule, comprising the steps of : a) selecting a primer pair defining the amplicon; b) selecting a specific sequence of 10 to 100 nucleotides within the amplicon, such that said specific sequence defines two non-complementary ends of the amplicon; c) defining the capture molecule having a capture portion that is complementary to the specific sequence selected in step b), and a spacer portion comprising at least 20 nucleotides; d) identifying among the two non- complementary ends of the amplicon a spacer end and a non-spacer end, respectively, such that the spacer end is non-complementary to the spacer portion of the capture molecule, and said spacer end exceeds said non-spacer end by at least 50 bases.
摘要翻译：本发明涉及设计和/或制备多核苷酸捕获分子的方法，所述多核苷酸捕获分子用于检测具有一条链的扩增子，所述扩增子用作在所述捕获分子上杂交后待检测和/或定量的靶，其包括以下步骤：a）选择限定扩增子的引物对; b）在扩增子内选择10至100个核苷酸的特定序列，使得所述特异性序列定义扩增子的两个非互补末端; c）限定具有与步骤b）中选择的特定序列互补的捕获部分的捕获分子和包含至少20个核苷酸的间隔部分; d）分别在扩增子的两个非互补末端之间鉴定间隔物末端和非间隔端，使得间隔物末端与捕获分子的间隔部分不互补，并且所述间隔物末端超过所述非间隔子末端，至少50个碱基。

7. 发明申请

WO2007131999A1 LID FOR PCR VESSEL COMPRISING PROBES PERMITTING PCR AMPLIFICATION AND DETECTION OF THE PCR PRODUCT BY HYBRIDISATION WITHOUT OPENING THE PCR VESSEL 审中-公开
标题翻译：用于包含探针的PCR载体允许PCR扩增和通过在不打开PCR载体的情况下进行杂交检测PCR产物
公开(公告)号：WO2007131999A1
公开(公告)日：2007-11-22
申请号：PCT/EP2007/054687
申请日：2007-05-15
申请人： EPPENDORF ARRAY TECHNOLOGIES S.A. , REMACLE, José , ALEXANDRE, Isabelle , SEIPPEL, Martin , BUELOW, Sven
发明人： REMACLE, José , ALEXANDRE, Isabelle , SEIPPEL, Martin , BUELOW, Sven
IPC分类号： B01L3/00 , C12Q1/68
CPC分类号： B01L3/50853 , B01L3/50851 , B01L2300/046 , B01L2300/0636 , B01L2300/0829
摘要： The present invention proposes a simple and effective lid being part of a multiwell device for performing simultaneous amplifications by PCR and detections of multiple target molecules on unlabeled capture molecules immobilized on the lid.
摘要翻译：本发明提出了一种简单有效的盖子，其是多孔装置的一部分，用于通过PCR同时扩增和检测固定在盖上的未标记的捕获分子上的多个靶分子。

8. 发明申请

WO2007131995A1 REACTION CHAMBER FOR REAL TIME PCR COMPRISING CAPTURE PROBES AND PERMITTING DETECTION OF THE PCR PRODUCT BY HYBRIDISATION WITHOUT OPENING THE PCR VESSEL 审中-公开
标题翻译：反应室用于包含捕获探针的实时PCR，并且通过在不打开PCR载体的情况下通过杂交来允许PCR产物的检测
公开(公告)号：WO2007131995A1
公开(公告)日：2007-11-22
申请号：PCT/EP2007/054663
申请日：2007-05-14
申请人： EPPENDORF ARRAY TECHNOLOGIES S.A. , REMACLE, José , ALEXANDRE, Isabelle , KOEHN, Heinz , SEIPPEL, Martin
发明人： REMACLE, José , ALEXANDRE, Isabelle , KOEHN, Heinz , SEIPPEL, Martin
IPC分类号： C12Q1/68 , B01J19/00
CPC分类号： B01L3/50825 , B01J19/0046 , B01J2219/00283 , B01J2219/00315 , B01J2219/00333 , B01J2219/00511 , B01J2219/00585 , B01J2219/00596 , B01J2219/00608 , B01J2219/0061 , B01J2219/00612 , B01J2219/00635 , B01J2219/00641 , B01J2219/00659 , B01J2219/00702 , B01J2219/00722 , B01L3/502715 , B01L3/50851 , B01L3/50853 , B01L7/52 , B01L2300/046 , B01L2300/0636 , B01L2300/0654 , B01L2300/0803 , B01L2400/0409 , B01L2400/0457
摘要： A reaction chamber (1) for real-time PCR configured for containing a PCR solution (3), said chamber having a capture molecule (4) immobilized on the chamber inner surface, said surface being configured to be in intermittent contact with the PCR solution (3), and a window (7) permitting detection of a signal from said inner surface by a reader (6) located outside the chamber, and wherein the immobilized capture molecule (4) is not labeled.
摘要翻译：一种用于实时PCR的反应室（1），用于容纳PCR溶液（3），所述室具有固定在室内表面上的捕获分子（4），所述表面被配置为与PCR溶液间歇接触（3）和窗口（7），允许通过位于室外部的读取器（6）检测来自所述内表面的信号，并且其中固定的捕获分子（4）未被标记。

9. 发明申请

WO1994006933A1 CONJUGATE FOR THE DETECTION AND/OR ASSAY OF A BIOLOGICAL COMPOUND 审中-公开
标题翻译：结合生物化学的检测和/或测定
公开(公告)号：WO1994006933A1
公开(公告)日：1994-03-31
申请号：PCT/BE1993000063
申请日：1993-09-21
申请人： LAMBDATECH S.A. , REMACLE, José , RENTIER, Bernard , ALEXANDRE, Isabelle , MORRIS, Philippe , ZAMMATTEO, Nathalie
发明人： LAMBDATECH S.A.
IPC分类号： C12Q01/68
CPC分类号： G01N33/535 , C12Q1/6816 , C12Q1/708
摘要： Novel conjugate for the detection and/or assay of a biological compound, consisting of a kinase or a dehydrogenase, the sulphur groups being preferably reduced beforehand, and capable of producing an intermediate compound for use in a bioluminescence system, preferably by means of a luciferase. Said enzyme is coupled to a ligand that was previously activated by a coupling agent and is capable of specifically bonding to said biological compound. The invention also concerns a process for the production of said conjugate, the application of said conjugate in detection and/or assay processes and a diagnostic kit containing same.
摘要翻译：用于由激酶或脱氢酶组成的生物化合物的检测和/或测定的新型缀合物，硫基优选预先减少，并且能够生产用于生物发光系统的中间体化合物，优选通过荧光素酶。所述酶与先前由偶联剂活化并能够特异性结合所述生物化合物的配体偶联。本发明还涉及生产所述缀合物的方法，所述缀合物在检测和/或测定过程中的应用以及包含其的诊断试剂盒。

10. 发明申请

WO2007132002A1 IDENTIFICATION AND QUANTIFICATION OF A PLURALITY OF BIOLOGICAL (MICRO)ORGANISMS OR THEIR COMPONENTS 审中-公开
标题翻译：生物多样性（微生物）或其组分的识别和定量
公开(公告)号：WO2007132002A1
公开(公告)日：2007-11-22
申请号：PCT/EP2007/054696
申请日：2007-05-15
申请人： EPPENDORF ARRAY TECHNOLOGIES S.A. , REMACLE, José , ALEXANDRE, Isabelle , KOEHN, Heinz , MARGAINE, Sylvian , HUSAR, Dieter , ZAMMATEO, Nathalie
发明人： REMACLE, José , ALEXANDRE, Isabelle , KOEHN, Heinz , MARGAINE, Sylvian , HUSAR, Dieter , ZAMMATEO, Nathalie
IPC分类号： C12Q1/68
CPC分类号： B01L3/50851 , B01L7/52 , B01L2300/0636 , B01L2300/0803 , B01L2300/0819 , C12Q1/686 , C12Q2561/113
摘要： Disclosed is a system and method conducting real-time PCR. Unlabeled capture molecules of a specific design are immobilized on a solid support, and contacted with amplicons produced in one or more PCR cycles. Detection of amplicons may take place during or between the PCR cycles while the solid support is in fluidic contact with the PCR solution. In an alternate embodiment detection of the amplicons takes place when the solid support is not in fluidic contact with the PCR solution. The method is suitable for the simultaneous detection and quantification of closely homologous target molecules.
摘要翻译：公开了进行实时PCR的系统和方法。将具体设计的未标记的捕获分子固定在固体支持物上，并与在一个或多个PCR循环中产生的扩增子接触。扩增子的检测可以在PCR循环期间或之间进行，而固体支持物与PCR溶液流体接触。在替代实施方案中，当固体支持物不与PCR溶液流体接触时，扩增子的检测发生。该方法适用于同时检测和定量分析近似同源的靶分子。

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