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1. 发明申请

WO2004040001A3 MICROFLUIDIC NUCLEIC ACID ANALYSIS 审中-公开
标题翻译：微流核酸分析
公开(公告)号：WO2004040001A3
公开(公告)日：2004-07-22
申请号：PCT/US0331412
申请日：2003-10-02
申请人： CALIFORNIA INST OF TECHN , HONG JONG WOOK , STUDER VINCENT , ANDERSON W FRENCH , QUAKE STEPHEN R , LEADBETTER JARED
发明人： HONG JONG WOOK , STUDER VINCENT , ANDERSON W FRENCH , QUAKE STEPHEN R , LEADBETTER JARED
IPC分类号： B01L3/00 , C12M1/34 , C12Q1/68 , C07H19/00 , C07H21/02 , C07H21/04 , C12P19/34
CPC分类号： B01L3/502715 , B01L3/50273 , B01L3/502738 , B01L3/502761 , B01L2200/10 , B01L2300/0809 , B01L2300/0816 , B01L2300/0877 , B01L2300/1827 , B01L2400/0481 , B01L2400/0655 , C12Q1/6806 , C12Q2565/629
摘要： Nucleic acid from cells and viruses sampled from a variety of environments may purified and expressed utilizing microfluidic techniques. In accordance with one embodiment of the present invention, individual or small groups of cells or viruses may be isolated in microfluidic chambers by dilution, sorting, and/or segmentation. The isolated cells or viruses may be lysed directly in the microfluidic chamber, and the resulting nucleic acid purified by exposure to affinity beads. Subsequent elution of the purified nucleic acid may be followed by ligation and cell transformation, all within the same microfluidic chip. In one specific application, cell isolation, lysis, and nucleic acid purification may be performed utilizing a highly parallelized microfluidic architecture to construct gDNA and cDNA libraries.
摘要翻译：来自从各种环境取样的细胞和病毒的核酸可以利用微流体技术纯化和表达。根据本发明的一个实施方案，可通过稀释，分选和/或分割在微流体室中分离单个或小组的细胞或病毒。分离的细胞或病毒可以直接在微流体室中裂解，并且通过暴露于亲和珠来纯化得到的核酸。随后纯化的核酸的洗脱之后可以连接和细胞转化，全部在相同的微流体芯片内。在一个具体应用中，细胞分离，裂解和核酸纯化可以使用高度并行的微流体结构来构建gDNA和cDNA文库。

2. 发明申请

WO2005060393A3 MICROFLUIDIC LARGE SCALE INTEGRATION 审中-公开
标题翻译：微流化大规模集成
公开(公告)号：WO2005060393A3
公开(公告)日：2006-03-09
申请号：PCT/US2004025946
申请日：2004-08-10
申请人： CALIFORNIA INST OF TECHN , STUDER VINCENT , QUAKE STEPHEN R , ANDERSON W FRENCH , MAERKL SEBASTIAN J
发明人： STUDER VINCENT , QUAKE STEPHEN R , ANDERSON W FRENCH , MAERKL SEBASTIAN J
IPC分类号： F15C5/00 , F16K7/04 , F16K11/20
CPC分类号： F15C5/00 , G06F17/5018 , G06F2217/16
摘要： Using basic physical arguments, a design and method for the fabrication of microfluidic valves using multilayer soft lithography is presented. Embodiments of valves in accordance with the present invention feature elastomer membrane portions of substantially constant thickness, allowing the membranes to experience similar resistance to an applied pressure across their entire width. Such on-off valves fabricated with upwardly- or downwardly-deflectable membranes can have extremely low actuation pressures, and can be used to implement active functions such as pumps and mixers in integrated microfluidic chips. Valve performance was characterized by measuring both the actuation pressure and flow resistance over a wide range of design parameters, and comparing them to both finite element simulations and alternative valve geometries.
摘要翻译：使用基本的物理参数，提出了使用多层软光刻制造微流体阀的设计和方法。根据本发明的阀的实施例具有基本恒定厚度的弹性体膜部分，允许膜在其整个宽度上经受类似的施加压力的阻力。用向上或向下可偏转膜制造的这种开关阀可以具有非常低的致动压力，并且可以用于实现积分功能，例如集成微流体芯片中的泵和混合器。阀性能的特征在于在宽范围的设计参数下测量致动压力和流动阻力，并将其与有限元模拟和替代阀几何形状进行比较。

3. 发明申请

WO2004028955A3 MICROFLUIDIC LARGE SCALE INTEGRATION 审中-公开
标题翻译：微型大规模集成
公开(公告)号：WO2004028955A3
公开(公告)日：2005-05-12
申请号：PCT/US0330335
申请日：2003-09-24
申请人： CALIFORNIA INST OF TECHN , MAERKL SEBASTIAN J , THORSEN TODD A , BAO XIAOYAN , QUAKE STEPHEN R , STUDER VINCENT
发明人： MAERKL SEBASTIAN J , THORSEN TODD A , BAO XIAOYAN , QUAKE STEPHEN R , STUDER VINCENT
IPC分类号： B01L3/00 , F15C5/00 , F16K11/20 , F16K99/00 , B81B1/00 , B81B3/00
CPC分类号： C12Q1/28 , B01L3/5025 , B01L3/50273 , B01L3/502738 , B01L2300/0861 , B01L2300/0887 , B01L2400/0481 , B01L2400/0655 , B81B2201/0214 , B81B2201/054 , B81B2201/07 , B81C1/00119 , F15C5/00 , F16K11/20 , F16K99/0001 , F16K99/0015 , F16K99/0059 , F16K2099/0074 , F16K2099/0076 , F16K2099/0078 , F16K2099/008 , F16K2099/0084 , F16K2099/0094 , Y10T137/0318 , Y10T137/0329 , Y10T137/2224 , Y10T137/85938 , Y10T137/87249
摘要： High-density microfluidic chips contain plumbing networks with thousands of micromechanical valves and hundreds of individually addressable chambers. These fluidic devices are analogous to electronic integrated circuits fabricated using large scale integration (LSI). A component of these networks is the fluidic multiplexor, which is a combinatorial array of binary valve patterns that exponentially increases the processing power of a network by allowing complex fluid manipulations with a minimal number of inputs. These integrated microfluidic networks can be used to construct a variety of highly complex microfluidic devices, for example the microfluidic analog of a comparator array, and a microfluidic memory storage device resembling electronic random access memories.
摘要翻译：高密度微流控芯片包含具有数千个微机械阀门和数百个单独可寻址腔室的管道网络。这些流体装置类似于使用大规模集成（LSI）制造的电子集成电路。这些网络的一个组件是流体多路复用器，它是二进制阀模式的组合阵列，通过允许使用最少数量的输入的复杂流体操纵来指数地增加网络的处理能力。这些集成的微流体网络可用于构建各种高度复杂的微流体装置，例如比较器阵列的微流体模拟装置和类似于电子随机存取存储器的微流体存储器存储装置。

4. 发明申请

WO2005076832A3 METHOD FOR MANUFACTURING SINGLE WALL CARBON NANOTUBE TIPS 审中-公开
标题翻译：制造单壁碳纳米管提示的方法
公开(公告)号：WO2005076832A3
公开(公告)日：2007-09-20
申请号：PCT/US2005002668
申请日：2005-02-01
申请人： CALIFORNIA INST OF TECHN , COLLIER CHARLES PATRICK , MA ZIYANG , QUAKE STEPHEN R , SHAPIRO IAN R , WADE LAWRENCE A
发明人： COLLIER CHARLES PATRICK , MA ZIYANG , QUAKE STEPHEN R , SHAPIRO IAN R , WADE LAWRENCE A
IPC分类号： G21K7/00 , C01B31/02 , G01Q10/00 , G01Q60/24 , G01Q60/38 , G01Q70/10 , G01Q70/12 , G01R3/00
CPC分类号： C01B32/162 , C01B2202/02 , C01B2202/34 , C01B2202/36 , G01Q60/38 , G01Q70/12 , G01R3/00
摘要： A method for fabricating assembled structures. The method includes providing a tip structure, which has a first end, a second end, and a length defined between the first end and the second end. The second end is a free end. The method includes attaching a nano-sized structure along a portion of the length of the tip structure to extend a total length of the tip structure to include the length of the tip structure and a first length associated with the nano-sized structure. The method includes shortening the nano-sized structure from the first length to a second length. The method also includes pushing the nano-sized structure in a direction parallel to the second length to reduce the second length to a third length of the nano-sized structure along the direction parallel to the second length to cause the nano-sized structure to move along a portion of the length of the tip structure.
摘要翻译：一种组装结构的制造方法。该方法包括提供尖端结构，其具有第一端，第二端和限定在第一端和第二端之间的长度。第二端是自由端。该方法包括沿着尖端结构的长度的一部分附接纳米尺寸结构以延伸尖端结构的总长度以包括尖端结构的长度和与纳米尺寸结构相关联的第一长度。该方法包括将纳米尺寸结构从第一长度缩短到第二长度。该方法还包括沿平行于第二长度的方向推动纳米尺寸结构，以沿平行于第二长度的方向将纳米尺寸结构的第二长度减小到第三长度，以使纳米尺寸结构移动沿着尖端结构的长度的一部分。

5. 发明申请

WO2005030822A3 PHOTOCURABLE PERFLUOROPOLYETHERS FOR USE AS NOVEL MATERIALS IN MICROFLUIDIC DEVICES 审中-公开
标题翻译：用于微流体装置中的新型材料的可光热全氟聚醚
公开(公告)号：WO2005030822A3
公开(公告)日：2007-02-22
申请号：PCT/US2004031274
申请日：2004-09-23
申请人： UNIV NORTH CAROLINA , CALIFORNIA INST OF TECHN , DESIMONE JOSEPH M , ROLLAND JASON P , QUAKE STEPHEN R , SCHORZMAN DEREK A , YARBROUGH JASON , VAN DAM MICHAEL
发明人： DESIMONE JOSEPH M , ROLLAND JASON P , QUAKE STEPHEN R , SCHORZMAN DEREK A , YARBROUGH JASON , VAN DAM MICHAEL
IPC分类号： C08J5/20 , B05D5/12 , B81B1/00 , C08G20060101 , H01M4/88 , H01M8/10
CPC分类号： B01L3/502707 , B01L3/0268 , B01L3/502738 , B01L7/52 , B01L2200/12 , B01L2300/0816 , B01L2400/0481 , B01L2400/0655 , B81B1/006 , B81B2201/058 , B81B2203/0323 , B81C2201/034 , B82Y10/00 , B82Y40/00 , C08G18/5015 , C08G18/8116 , C08G65/007 , C08G65/33348 , C08G2650/48 , C09D163/00 , C09D171/02 , F16K99/0001 , F16K99/0015 , F16K99/0026 , F16K99/0034 , F16K99/0036 , F16K99/0042 , F16K99/0046 , F16K99/0051 , F16K99/0059 , F16K99/0061 , F16K99/0065 , F16K2099/008 , F16K2099/0084 , F16K2099/0086 , G03F7/0002 , Y10T137/0396 , Y10T428/24479 , Y10T428/249954 , Y10T428/3154
摘要： A solvent-resistant microfluidio device (MD) is fabricated from a functionalized, photo-curable perfluoropolyether (PFPE). In one embodiment, a polymeric precursor (PP), comprising PFPE, is disposed on a patterned surface (PS) of a substrate (S), having raised protrusions (P). Ultraviolet light (UV) is applied to yield a patterned layer (PL) of photo-cured PFPE having recesses (R) comprising at least one channel (CH). Patterned layer (PL) is removed from patterned surface (PS) of substrate (S) to yield microfluidic device (MD).
摘要翻译：耐溶剂微流体装置（MD）由功能化的光可固化全氟聚醚（PFPE）制成。在一个实施方案中，包含PFPE的聚合物前体（PP）设置在具有凸起（P）的基底（S）的图案化表面（PS）上。施加紫外光（UV）以产生具有包含至少一个通道（CH）的凹陷（R）的光固化PFPE的图案化层（PL）。从基板（S）的图案化表面（PS）去除图形层（PL）以产生微流体装置（MD）。

6. 发明申请

WO2007117346A3 PROGRAMMING MICROFLUIDIC DEVICES WITH MOLECULAR INFORMATION 审中-公开
标题翻译：编写具有分子信息的微流体装置
公开(公告)号：WO2007117346A3
公开(公告)日：2008-05-02
申请号：PCT/US2007002128
申请日：2007-01-26
申请人： CALIFORNIA INST OF TECHN , MAERKL SEBASTIAN J , QUAKE STEPHEN R
发明人： MAERKL SEBASTIAN J , QUAKE STEPHEN R
IPC分类号： B01J8/00
CPC分类号： G01N33/543 , B01J2219/0043 , B01L3/5027 , B01L3/502707 , B01L3/502738 , B01L2300/0816 , B01L2300/0819 , B01L2300/0861 , B01L2300/123 , B01L2400/0481 , B01L2400/0655 , G01N33/5302 , G01N33/54393
摘要： The invention provides a microfluidic device having a plurality of chambers each containing separately deposited reagents. The invention also provides an efficient PCR-based method for producing a linear expression template. The invention also provides methods for analyzing interactions between molecules, involving flow-deposition of expression templates on the substrate of chambers in a microfluidic device, and expressing proteins from the templates.
摘要翻译：本发明提供了一种具有多个室的微流体装置，每个腔室均含有单独沉积的试剂。本发明还提供了一种用于产生线性表达模板的有效的基于PCR的方法。本发明还提供了用于分析分子之间的相互作用的方法，其涉及在微流体装置中的室的底物上的表达模板的流沉积，以及来自模板的蛋白质。

7. 发明申请

WO2005069980A3 MICROFLUIDIC CHEMOSTAT AND A METHOD TO PREVENT BIOFILM FORMATION WITH SAME 审中-公开
标题翻译：微流化学法和一种预防生物体形成的方法
公开(公告)号：WO2005069980A3
公开(公告)日：2005-09-29
申请号：PCT/US2005000211
申请日：2005-01-05
申请人： CALIFORNIA INST OF TECHN , BALAGADDE FREDERICK , HANSEN CARL L , KARTALOV EMIL , QUAKE STEPHEN R
发明人： BALAGADDE FREDERICK , HANSEN CARL L , KARTALOV EMIL , QUAKE STEPHEN R
IPC分类号： B01L3/00 , C12M1/00 , C12M1/113 , C12M1/18 , C12M3/00 , C12P19/04 , C12P19/34 , C12Q1/02 , C12Q1/68
CPC分类号： C12P19/04 , B01L3/5027 , B01L3/502738 , B01L2300/0861 , B01L2300/0877 , B01L2300/10 , B01L2300/163 , B01L2400/0481 , B01L2400/0655 , C12M23/16 , C12M23/34 , C12M39/00 , C12P1/00
摘要： A chemostat (Figure 1) that includes a growth chamber having a plurality of compartments, where each of the compartments may be fluidly isolated from the rest of the growth chamber by one or more actuatable valves. The chemostat may also include a nutrient supply-line to supply growth medium to the growth chamber (s), and an output port to remove fluids from the growth chamber. Also, presented is a method to prevent biofilm formation in a growth chamber of the chemostat. The method may include the step of adding a lytic agent to an isolated portion of the growth chamber and re-uniting the isolated portion with the rest of the growth chamber.
摘要翻译：一种恒温器（图1），其包括具有多个室的生长室，其中每个室可以通过一个或多个可致动阀与生长室的其余部分流体隔离。恒化器还可以包括向生长室供应生长培养基的营养供应线以及从生长室去除流体的输出端口。还提出了防止生长膜在恒化器生长室中形成的方法。该方法可以包括将溶解剂添加到生长室的分离部分并将分离的部分与生长室的其余部分重新结合的步骤。

8. 发明申请

WO0175176A3 METHODS AND SYSTEMS FOR MOLECULAR FINGERPRINTING 审中-公开
标题翻译：用于分子指纹的方法和系统
公开(公告)号：WO0175176A3
公开(公告)日：2003-08-07
申请号：PCT/US0110881
申请日：2001-04-04
申请人： CALIFORNIA INST OF TECHN , QUAKE STEPHEN R , CHOU HOU-PU
发明人： QUAKE STEPHEN R , CHOU HOU-PU
IPC分类号： C12Q1/68 , B01L3/00
CPC分类号： C12Q1/683
摘要： This invention relates in general to a method for molecular fingerprinting. The method can be used for forensic identification (e.g. DNA fingerprinting, especially by VNTR), bacteria typing, and human/animal pathogen diagnosis. More particularly, molecules such as polynucleotides (e.g. DNA) can be assessed or sorted by size in a microfabricated device that analyzes the polynucleotides according to restriction fragment length polymorphism. In a microfabricated device according to the invention, DNA fragments or other molecules can be rapidly and accurately typed using relatively small samples, by measuring for example the signal of an optically-detectable (e.g., fluorescent) reporter associated with the polynucleotide fragments.
摘要翻译：本发明一般涉及分子指纹图谱的方法。该方法可用于法医鉴定（例如DNA指纹图谱，特别是VNTR），细菌分型和人/动物病原体诊断。更具体地，可以按照限制性片段长度多态性分析多核苷酸的微制造装置中的大小来评估或分类分子，例如多核苷酸（例如DNA）。在根据本发明的微制造装置中，通过测量例如与多核苷酸片段相关的可光学检测（例如，荧光）报告基因的信号，可以使用相对小的样品快速和精确地分类DNA片段或其他分子。

9. 发明申请

WO0101025A9 MICROFABRICATED ELASTOMERIC VALVE AND PUMP SYSTEMS 审中-公开
标题翻译：微型弹药阀和泵系统
公开(公告)号：WO0101025A9
公开(公告)日：2002-07-25
申请号：PCT/US0017740
申请日：2000-06-27
申请人： CALIFORNIA INST OF TECHN , UNGER MARC A , CHOU HOU-PU , THORSEN TODD A , SCHERER AXEL , QUAKE STEPHEN R
发明人： UNGER MARC A , CHOU HOU-PU , THORSEN TODD A , SCHERER AXEL , QUAKE STEPHEN R
IPC分类号： G03F7/20 , B01L3/00 , B81B1/00 , B81B3/00 , B81B7/00 , B81C1/00 , B81C99/00 , C12Q1/68 , F04B17/00 , F04B35/04 , F04B43/04 , F15C5/00 , F16K7/00 , F16K17/02 , F16K99/00
CPC分类号： B29C51/00 , B01L3/502707 , B01L3/50273 , B01L3/502738 , B01L2300/0861 , B01L2300/0887 , B01L2300/123 , B01L2400/046 , B01L2400/0481 , B01L2400/0655 , B33Y80/00 , B81B5/00 , B81B2201/036 , B81B2201/054 , B81C1/00119 , B81C2201/019 , B81C2201/034 , C12Q1/6874 , F04B43/043 , F15C1/06 , F15C5/00 , F16K11/20 , F16K13/00 , F16K31/126 , F16K99/0001 , F16K99/0015 , F16K99/0046 , F16K99/0048 , F16K99/0051 , F16K99/0059 , F16K2099/0074 , F16K2099/0076 , F16K2099/0078 , F16K2099/008 , F16K2099/0094 , Y10T137/0491 , Y10T137/0497 , Y10T137/2224 , Y10T137/87249 , Y10T156/10 , Y10T428/24479 , Y10T428/24744 , C12Q2535/125
摘要： A method of fabricating an elastomeric structure, comprising : forming a first elastomeric layer on top of a first micromachined mold, the first micromachined mold having a first raised protrusion which forms a first recess extending along a bottom surface of the first elastomeric layer; forming a second elastomeric layer on top of a second micromachined mold, the second micromachined mold having a second raised protrusion which forms a second recess extending along a bottom surface of the second elastomeric layer; bonding the bottom surface of the second elastomeric layer onto a top surface of the first elastomeric layer such that a control channel forms in the second recess between the first and second elastomeric layers; and positioning the first elastomeric layer on top of a planar substrate such that a flow channel forms in the first recess between the first elastomeric layer and the planar substrate.
摘要翻译：一种制造弹性体结构的方法，包括：在第一微加工模具的顶部上形成第一弹性体层，所述第一微加工模具具有形成沿所述第一弹性体层的底表面延伸的第一凹槽的第一凸起突起; 在第二微加工模具的顶部上形成第二弹性体层，所述第二微加工模具具有第二凸起突起，所述第二凸起突起形成沿所述第二弹性体层的底表面延伸的第二凹槽; 将第二弹性体层的底表面粘合到第一弹性体层的顶表面上，使得控制通道在第一和第二弹性体层之间的第二凹部中形成; 以及将第一弹性体层定位在平面基底的顶部上，使得流动通道在第一弹性体层和平面基底之间的第一凹部中形成。

10. 发明申请

WO2008097841A3 SURFACE CHEMISTRY AND DEPOSITION TECHNIQUES 审中-公开
标题翻译：表面化学和沉积技术
公开(公告)号：WO2008097841A3
公开(公告)日：2008-10-09
申请号：PCT/US2008052796
申请日：2008-02-01
申请人： CALIFORNIA INST OF TECHN , UNIV STANFORD , UNIV CHICAGO , SCHWARTZ JERROD , QUAKE STEPHEN R , MRKSICH MILAN
发明人： SCHWARTZ JERROD , QUAKE STEPHEN R , MRKSICH MILAN
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6874 , G01N33/54353 , G01N33/54393 , Y10T436/143333
摘要： Surface chemistries for the visualization of labeled single molecules (analytes) with improved signal-to-noise properties are provided. To be observed, analyte molecules are bound to surface attachment features that are spaced apart on the surface such that when the analytes are labeled adjacent analytes are optically resolvable from each other. One way to express this concept is that binding elements should be spaced apart such that the Guassian point spread functions of adjacent labels do not overlap. Another way of expressing this concept is that the surface binding elements should be spaced apart by a distance equal to at least the diffraction limit for an optical label attached to the bound analytes.
摘要翻译：提供了具有改善的信噪比的用于可视化标记的单分子（分析物）的表面化学。为了观察，分析物分子结合到在表面上间隔开的表面附着特征，使得当分析物被标记时，相邻的分析物彼此光学可分辨。表达这种概念的一种方式是，绑定元素应该间隔开，使得相邻标签的高斯点扩散函数不重叠。表达这一概念的另一种方式是表面结合元件应间隔开至少等于附着于结合分析物的光学标记的衍射极限的距离。

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