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    • 2. 发明申请
    • PRODUCTION OF ACID SOLUBLE SOY PROTEIN ISOLATES (
    • 生产可溶性大豆蛋白分离物(“S800”)
    • WO2011000097A1
    • 2011-01-06
    • PCT/CA2010/001016
    • 2010-06-30
    • BURCON NUTRASCIENCE (MB) CORP.SEGALL, Kevin, I.SCHWEIZER, MartinGREEN, Brent, E.MEDINA, SarahGOSNELL, Brandy
    • SEGALL, Kevin, I.SCHWEIZER, MartinGREEN, Brent, E.MEDINA, SarahGOSNELL, Brandy
    • A23J1/14A23J3/16A23L2/02
    • A23L2/66A23L33/185
    • A soy protein product having a protein content of at least about 60 wt% (N x 6.25) d.b., preferably an isolate having a protein content of at least about 90 wt% (N x 6.25) d.b., is formed by extracting a soy protein source with water to form an aqueous protein solution having a pH of about 1.5 to about 11, preferably about 5 to about 7, and separating the resulting aqueous protein solution from residual soy protein source. The protein concentration of the aqueous protein solution is increased to about 50 to about 400 g/L while the ionic strength is maintained substantially constant by using a selective membrane technique. The resulting concentrated protein solution is optionally diafiltered and a calcium salt, preferably calcium chloride, is added to the concentrated and optionally diafiltered protein solution to a conductivity of 5 to about 30 mS. Precipitate formed as a result of the calcium salt addition is removed and the resulting clarified retentate is diluted into about 2 to about 20 volumes of water prior to acidification to a pH of about 1.5 to about 4.4 to produce an acidified clear protein solution. The acidified clear protein solution is then concentrated and optionally diafiltered and optionally dried. Variations of this procedure can be used to produce a soy protein product which is soluble, transparent and heat stable in acidic aqueous environments.
    • 蛋白质含量至少约为60重量%(N×6.25)db,优选具有至少约90重量%(N×6.25)db蛋白质含量的分离物的大豆蛋白产物是通过提取大豆蛋白 用水形成具有约1.5至约11,优选约5至约7的pH的蛋白质水溶液,并将所得蛋白质水溶液与残留的大豆蛋白源分离。 通过使用选择性膜技术,蛋白质水溶液的蛋白质浓度增加至约50至约400g / L,同时离子强度基本上保持恒定。 任选地将所得浓缩的蛋白质溶液进行渗滤,并将钙盐,优选氯化钙加入到浓缩和任选的渗滤的蛋白质溶液中至5至约30mS的电导率。 去除由钙盐加入而形成的沉淀物,并将所得澄清的滞留物在酸化至约1.5至约4.4的pH之前稀释成约2至约20体积的水,以产生酸化的澄清蛋白质溶液。 然后浓缩酸化的透明蛋白质溶液,任选地进行渗滤并任选地干燥。 该方法的变化可以用于生产在酸性水性环境中是可溶的,透明的和热稳定的大豆蛋白产品。
    • 4. 发明申请
    • PRODUCTION OF SOY PROTEIN PRODUCT USING CALCIUM CHLORIDE EXTRACTION (
    • 使用氯化钙萃取生产大豆蛋白产品(“S702 / S7300 / S7200 / S7301”)
    • WO2010091509A1
    • 2010-08-19
    • PCT/CA2010/000189
    • 2010-02-11
    • BURCON NUTRASCIENCE (MB) CORP.SEGALL, Kevin, I.SCHWEIZER, MartinGREEN, Brent, E.MEDINA, SarahGOSNELL, Brandy
    • SEGALL, Kevin, I.SCHWEIZER, MartinGREEN, Brent, E.MEDINA, SarahGOSNELL, Brandy
    • A23J3/16A23C11/10A23J1/14A23L2/46A23L2/74
    • A23J1/14A23J3/16A23L2/39A23L2/66A23L33/185A23V2002/00A23V2300/34A23V2300/40
    • A soy protein product having a protein content of at least about 60 wt% (N x 6.25) d.b., preferably a soy protein isolate having a protein content of at least about 90 wt% (N x 6.25) d.b., is prepared from a soy protein source material by extraction of the soy protein source material with an aqueous calcium salt solution, preferably calcium chloride solution, to cause solubilization of soy protein from the protein source and to form an aqueous soy protein solution, separating the aqueous soy protein solution from residual soy protein source, concentrating the aqueous soy protein solution while maintaining the ionic strength substantially constant by using a selective membrane technique, optionally diafiltering the concentrated soy protein solution, and drying the concentrated and optionally diafiltered soy protein solution. Alternatively, the concentrated and optionally diafiltered soy protein solution may be diluted into water to cause the formation of a precipitate, separating the precipitate from the diluting water (supernatant), and drying the separated soy protein solution to form a soy protein product having a protein content of at least about 60 wt% (N x 6.25) d.b., preferably a soy protein isolate having a protein content of at least about 90 wt% (N x 6.25) d.b.. The supernatant may be processed to form soy protein products having a protein content of at least about 60 wt% (N x 6.25) d.b., preferably a soy protein isolate having a protein content of at least 90 wt% (N x 6.25) d.b. Alternatively, the precipitate from the dilution step and diluting water is acidified to resolubilize the precipitate and form a clear soy protein solution. The clear soy protein solution is concentrated while maintaining the ionic strength substantially constant by using a selective membrane technique followed by optional diafiltration and drying.
    • 蛋白质含量至少为约60重量%(N×6.25)db,优选蛋白质含量为至少约90重量%(N×6.25)db的大豆蛋白分离物的大豆蛋白产品由大豆制备 通过用钙盐水溶液(优选氯化钙溶液)提取大豆蛋白源材料来引起大豆蛋白质从蛋白质来源溶解并形成大豆蛋白水溶液,将大豆蛋白水溶液与残余物分离 大豆蛋白源,浓缩大豆蛋白水溶液,同时通过使用选择性膜技术保持离子强度基本上恒定,任选地过滤浓缩的大豆蛋白溶液,并干燥浓缩和任选的渗滤的大豆蛋白溶液。 或者,浓缩的和任选的渗滤的大豆蛋白溶液可以稀释成水以引起沉淀物的形成,将沉淀物与稀释水(上清液)分离,并干燥分离的大豆蛋白溶液以形成具有蛋白质的大豆蛋白产物 至少约60重量%(N×6.25)db的含量,优选具有至少约90重量%(N×6.25)db的蛋白质含量的大豆蛋白分离物,可以将上清液加工成具有 蛋白质含量为至少约60重量%(N×6.25)db,优选蛋白质含量至少为90重量%(N×6.25)db的大豆蛋白分离物 或者,将来自稀释步骤和稀释水的沉淀物酸化以使沉淀物再溶解并形成透明的大豆蛋白溶液。 将透明的大豆蛋白溶液浓缩,同时通过使用选择性膜技术,然后进行任选的渗滤和干燥来保持离子强度基本恒定。
    • 5. 发明申请
    • PREPARATION OF SOY PROTEIN ISOLATE USING CALCIUM CHLORIDE EXTRACTION (
    • 使用氯化钙提取制备分离的大豆蛋白(“S703 CIP”)
    • WO2012155242A1
    • 2012-11-22
    • PCT/CA2012/000443
    • 2012-05-09
    • BURCON NUTRASCIENCE (MB) CORP.SEGALL, Kevin I.SCHWEIZER, MartinGREEN, Brent E.MEDINA, SarahGOSNELL, Brandy
    • SEGALL, Kevin I.SCHWEIZER, MartinGREEN, Brent E.MEDINA, SarahGOSNELL, Brandy
    • A23J3/16
    • A23J1/14A23L2/66A23L33/185A23V2002/00A23V2200/33A23V2250/5488
    • A soy protein product having a protein content of at least about 60 wt% (N x 6.25) d.b., preferably an isolate having a protein content of at least about 90 wt% (N x 6.25) d.b., is formed by a procedure in which soy protein is extracted from a soy source material using an aqueous calcium chloride solution at low pH, generally about 1.5 to about 5, and separating the resulting aqueous soy protein solution from residual soy protein source. The resulting clarified aqueous soy protein solution may be diluted and the pH adjusted within the range of 1.5-5.0. The solution may be concentrated by ultrafiltration, diafiltered and then dried to provide the soy protein product. Alternatively, the concentrated and optionally diafiltered soy protein solution may be optionally adjusted in pH within the range of 1.5-7.0 then diluted into water to cause the formation of a precipitate, separating the precipitate from the diluting water (supernatant) and drying the separated soy protein to form a soy protein product having a protein content of at least about 60 wt% (N x 6.25) d.b., preferably a soy protein isolate having a protein content of at least about 90 wt% (N x 6.25) d.b.. The supernatant may be processed to form soy protein products having a protein content of at least about 60 wt% (N x 6.25) d.b., preferably a soy protein isolate having a protein content of at least 90 wt% (N x 6.25) d.b.. Alternatively, the precipitate from the dilution step may be re-solubilized in the diluting water by adjustment of the pH to resolubilize the precipitate and form a protein solution. The soy protein solution may be concentrated while maintaining the ionic strength substantially constant by using a selective membrane technique followed by optional diafiltration and drying. The soy protein product is soluble in acidic medium and produces transparent, heat stable solutions and hence may be used for protein fortification of soft drinks and sports drinks.
    • 蛋白质含量为至少约60重量%(N×6.25)db,优选具有至少约90重量%(N x 6.25)db的蛋白质含量的分离物的大豆蛋白产物通过以下步骤形成,其中 大豆蛋白质是使用氯化钙水溶液从大豆源材料中提取的,所述氯化钙水溶液在低pH(通常约1.5至约5)下,并将所得的大豆蛋白水溶液与残留的大豆蛋白源分离。 所得澄清的大豆蛋白水溶液可以稀释,pH调节在1.5-5.0的范围内。 可以通过超滤浓缩溶液,进行渗滤,然后干燥以提供大豆蛋白产物。 或者,浓缩的和任选的渗滤的大豆蛋白溶液可以任选地在1.5-7.0的范围内调节pH,然后稀释到水中以形成沉淀物,将沉淀物与稀释水(上清液)分离并干燥分离的大豆 蛋白质以形成具有至少约60重量%(N×6.25)db的蛋白质含量的大豆蛋白产物,优选具有至少约90重量%(N×6.25)db的蛋白质含量的大豆蛋白分离物,上清液 可以加工以形成蛋白质含量为至少约60重量%(N×6.25)db的蛋白质产物,优选具有至少90重量%(N×6.25)db的蛋白质含量的大豆蛋白分离物, 稀释步骤中的沉淀可以通过调节pH再溶解在稀释水中,以使沉淀物再溶解并形成蛋白质溶液。 可以通过使用选择性膜技术,然后任选的渗滤和干燥,将大豆蛋白溶液浓缩,同时保持离子强度基本恒定。 大豆蛋白产品可溶于酸性介质,产生透明,热稳定的溶液,因此可用于软饮料和运动饮料的蛋白质强化。
    • 7. 发明申请
    • PRODUCTION OF ACID SOLUBLE SOY PROTEIN ISOLATES (
    • 生产可溶性大豆蛋白分离物(“S700”)
    • WO2011000096A1
    • 2011-01-06
    • PCT/CA2010/001015
    • 2010-06-30
    • BURCON NUTRASCIENCE (MB) CORP.SCHWEIZER, MartinSEGALL, Kevin, I.GREEN, Brent, E.MEDINA, SarahGOSNELL, Brandy
    • SCHWEIZER, MartinSEGALL, Kevin, I.GREEN, Brent, E.MEDINA, SarahGOSNELL, Brandy
    • A23J1/14A23J3/16A23L2/02
    • A23L2/66A23J1/14A23L2/39A23L33/185A23V2002/00A23V2250/1578A23V2300/34A23V2300/38A23V2300/40
    • A soy protein product having a protein content of at least about 60 wt% (N x 6.25) d.b., preferably an isolate having a protein content of at least about 90 wt% (N x 6.25) d.b., is formed by extracting a soy protein source with a salt solution, preferably aqueous sodium chloride solution, to form an aqueous protein solution having a pH of about 1.5 to 11, preferably about 5 to about 7 and separating the resulting aqueous protein solution from residual soy protein source. The protein concentration of the aqueous protein solution is increased to about 50 to about 400 g/L while the ionic strength is maintained substantially constant by using a selective membrane technique. The resulting concentrated protein solution is optionally diafiltered and a calcium salt, preferably calcium chloride, is added to the concentrated and optionally diafiltered protein solution to a conductivity of 15 to about 85 mS. Precipitate formed as a result of the calcium salt addition is removed and the resulting clarified retentate is diluted into about 2 to about 20 volumes of water prior to acidification to a pH of about 1.5 to about 4.4 to produce an acidified clear protein solution. The acidified clear protein solution is then concentrated and optionally diafiltered and optionally dried. Variations of this procedure can be used to produce a soy protein product which is soluble, transparent and heat stable in acidic aqueous environments.
    • 蛋白质含量至少约为60重量%(N×6.25)db,优选具有至少约90重量%(N×6.25)db蛋白质含量的分离物的大豆蛋白产物是通过提取大豆蛋白 来源于盐溶液,优选氯化钠水溶液,以形成pH为约1.5至11,优选约5至约7的蛋白质水溶液,并将所得蛋白质水溶液与残留的大豆蛋白源分离。 通过使用选择性膜技术,蛋白质水溶液的蛋白质浓度增加至约50至约400g / L,同时离子强度基本上保持恒定。 任选地将所得浓缩的蛋白质溶液进行渗滤,并将钙盐(优选氯化钙)加入到浓缩和任选的渗滤的蛋白质溶液中至15至约85mS的电导率。 去除由钙盐加入而形成的沉淀物,并将所得澄清的滞留物在酸化至约1.5至约4.4的pH之前稀释成约2至约20体积的水,以产生酸化的澄清蛋白质溶液。 然后浓缩酸化的透明蛋白质溶液,任选地进行渗滤并任选地干燥。 该方法的变化可以用于生产在酸性水性环境中是可溶的,透明的和热稳定的大豆蛋白产品。