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    • 7. 发明申请
    • PRODUCTION OF ACID SOLUBLE SOY PROTEIN ISOLATES (
    • 生产可溶性大豆蛋白分离物(“S800”)
    • WO2011000097A1
    • 2011-01-06
    • PCT/CA2010/001016
    • 2010-06-30
    • BURCON NUTRASCIENCE (MB) CORP.SEGALL, Kevin, I.SCHWEIZER, MartinGREEN, Brent, E.MEDINA, SarahGOSNELL, Brandy
    • SEGALL, Kevin, I.SCHWEIZER, MartinGREEN, Brent, E.MEDINA, SarahGOSNELL, Brandy
    • A23J1/14A23J3/16A23L2/02
    • A23L2/66A23L33/185
    • A soy protein product having a protein content of at least about 60 wt% (N x 6.25) d.b., preferably an isolate having a protein content of at least about 90 wt% (N x 6.25) d.b., is formed by extracting a soy protein source with water to form an aqueous protein solution having a pH of about 1.5 to about 11, preferably about 5 to about 7, and separating the resulting aqueous protein solution from residual soy protein source. The protein concentration of the aqueous protein solution is increased to about 50 to about 400 g/L while the ionic strength is maintained substantially constant by using a selective membrane technique. The resulting concentrated protein solution is optionally diafiltered and a calcium salt, preferably calcium chloride, is added to the concentrated and optionally diafiltered protein solution to a conductivity of 5 to about 30 mS. Precipitate formed as a result of the calcium salt addition is removed and the resulting clarified retentate is diluted into about 2 to about 20 volumes of water prior to acidification to a pH of about 1.5 to about 4.4 to produce an acidified clear protein solution. The acidified clear protein solution is then concentrated and optionally diafiltered and optionally dried. Variations of this procedure can be used to produce a soy protein product which is soluble, transparent and heat stable in acidic aqueous environments.
    • 蛋白质含量至少约为60重量%(N×6.25)db,优选具有至少约90重量%(N×6.25)db蛋白质含量的分离物的大豆蛋白产物是通过提取大豆蛋白 用水形成具有约1.5至约11,优选约5至约7的pH的蛋白质水溶液,并将所得蛋白质水溶液与残留的大豆蛋白源分离。 通过使用选择性膜技术,蛋白质水溶液的蛋白质浓度增加至约50至约400g / L,同时离子强度基本上保持恒定。 任选地将所得浓缩的蛋白质溶液进行渗滤,并将钙盐,优选氯化钙加入到浓缩和任选的渗滤的蛋白质溶液中至5至约30mS的电导率。 去除由钙盐加入而形成的沉淀物,并将所得澄清的滞留物在酸化至约1.5至约4.4的pH之前稀释成约2至约20体积的水,以产生酸化的澄清蛋白质溶液。 然后浓缩酸化的透明蛋白质溶液,任选地进行渗滤并任选地干燥。 该方法的变化可以用于生产在酸性水性环境中是可溶的,透明的和热稳定的大豆蛋白产品。