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    • 2. 发明申请
    • PREPARATION OF SOY PROTEIN ISOLATE USING CALCIUM CHLORIDE EXTRACTION (
    • 使用氯化钙提取制备分离的大豆蛋白(“S703 CIP”)
    • WO2012155242A1
    • 2012-11-22
    • PCT/CA2012/000443
    • 2012-05-09
    • BURCON NUTRASCIENCE (MB) CORP.SEGALL, Kevin I.SCHWEIZER, MartinGREEN, Brent E.MEDINA, SarahGOSNELL, Brandy
    • SEGALL, Kevin I.SCHWEIZER, MartinGREEN, Brent E.MEDINA, SarahGOSNELL, Brandy
    • A23J3/16
    • A23J1/14A23L2/66A23L33/185A23V2002/00A23V2200/33A23V2250/5488
    • A soy protein product having a protein content of at least about 60 wt% (N x 6.25) d.b., preferably an isolate having a protein content of at least about 90 wt% (N x 6.25) d.b., is formed by a procedure in which soy protein is extracted from a soy source material using an aqueous calcium chloride solution at low pH, generally about 1.5 to about 5, and separating the resulting aqueous soy protein solution from residual soy protein source. The resulting clarified aqueous soy protein solution may be diluted and the pH adjusted within the range of 1.5-5.0. The solution may be concentrated by ultrafiltration, diafiltered and then dried to provide the soy protein product. Alternatively, the concentrated and optionally diafiltered soy protein solution may be optionally adjusted in pH within the range of 1.5-7.0 then diluted into water to cause the formation of a precipitate, separating the precipitate from the diluting water (supernatant) and drying the separated soy protein to form a soy protein product having a protein content of at least about 60 wt% (N x 6.25) d.b., preferably a soy protein isolate having a protein content of at least about 90 wt% (N x 6.25) d.b.. The supernatant may be processed to form soy protein products having a protein content of at least about 60 wt% (N x 6.25) d.b., preferably a soy protein isolate having a protein content of at least 90 wt% (N x 6.25) d.b.. Alternatively, the precipitate from the dilution step may be re-solubilized in the diluting water by adjustment of the pH to resolubilize the precipitate and form a protein solution. The soy protein solution may be concentrated while maintaining the ionic strength substantially constant by using a selective membrane technique followed by optional diafiltration and drying. The soy protein product is soluble in acidic medium and produces transparent, heat stable solutions and hence may be used for protein fortification of soft drinks and sports drinks.
    • 蛋白质含量为至少约60重量%(N×6.25)db,优选具有至少约90重量%(N x 6.25)db的蛋白质含量的分离物的大豆蛋白产物通过以下步骤形成,其中 大豆蛋白质是使用氯化钙水溶液从大豆源材料中提取的,所述氯化钙水溶液在低pH(通常约1.5至约5)下,并将所得的大豆蛋白水溶液与残留的大豆蛋白源分离。 所得澄清的大豆蛋白水溶液可以稀释,pH调节在1.5-5.0的范围内。 可以通过超滤浓缩溶液,进行渗滤,然后干燥以提供大豆蛋白产物。 或者,浓缩的和任选的渗滤的大豆蛋白溶液可以任选地在1.5-7.0的范围内调节pH,然后稀释到水中以形成沉淀物,将沉淀物与稀释水(上清液)分离并干燥分离的大豆 蛋白质以形成具有至少约60重量%(N×6.25)db的蛋白质含量的大豆蛋白产物,优选具有至少约90重量%(N×6.25)db的蛋白质含量的大豆蛋白分离物,上清液 可以加工以形成蛋白质含量为至少约60重量%(N×6.25)db的蛋白质产物,优选具有至少90重量%(N×6.25)db的蛋白质含量的大豆蛋白分离物, 稀释步骤中的沉淀可以通过调节pH再溶解在稀释水中,以使沉淀物再溶解并形成蛋白质溶液。 可以通过使用选择性膜技术,然后任选的渗滤和干燥,将大豆蛋白溶液浓缩,同时保持离子强度基本恒定。 大豆蛋白产品可溶于酸性介质,产生透明,热稳定的溶液,因此可用于软饮料和运动饮料的蛋白质强化。