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    • 6. 发明申请
    • NOVEL CANOLA PROTEIN ISOLATE
    • 新加拉瓜蛋白分离
    • WO2005067729A1
    • 2005-07-28
    • PCT/CA2005/000062
    • 2005-01-20
    • BURCON NUTRASCIENCE (MB) CORP.SCHWEIZER, MartinGREEN, Brent, E.SEGALL, Kevin, I.WILLARDSEN, Randy
    • SCHWEIZER, MartinGREEN, Brent, E.SEGALL, Kevin, I.WILLARDSEN, Randy
    • A23J1/14
    • C07K14/415A23J1/14A23J3/14
    • A novel canola protein isolate consisting predominantly of 2S canola protein and having improved solubility properties, has an increased proportion of 2S canola protein and a decreased proportion of 7S canola protein. The novel canola protein isolate is formed by heat treatment of aqueous supernatant from canola protein micelle formation and precipitation, to effect precipitation of 7S protein which is sedimented and removed. Alternatively, the novel canola protein isolate may be derived from a selective membrane procedure in which an aqueous canola protein solution containing 12S, 7S and 2S canola proteins is subjected to a first selective membrane technique to retain 12S and 7S canola proteins in a retentate, which is dried to provide a canola protein isolate consisting predominantly of 7S canola protein, and to permit 2S canola protein to pass through the membrane, the permeate is subjected to a second selective membrane technique to retain 2S canola protein and to permit low molecular weight contaminants to pass through the membrane, and the retentate from the latter membrane technique is dried.
    • 主要由2S卡诺拉蛋白组成并具有改善的溶解性质的新型卡诺拉蛋白分离物具有增加的2S卡诺拉蛋白的比例和7S卡诺拉蛋白的比例降低。 通过加热处理来自卡诺拉蛋白胶束形成和沉淀的水性上清液,形成沉淀和去除的7S蛋白沉淀,形成新的卡诺拉蛋白分离物。 或者,新的卡诺拉蛋白分离物可以衍生自选择性膜方法,其中含有12S,7S和2S卡诺拉蛋白质的低芥酸菜子蛋白溶液经受第一选择性膜技术以将12S和7S卡诺拉蛋白质保留在滞留物中, 干燥以提供主要由7S卡诺拉蛋白质组成的卡诺拉蛋白分离物,并允许2S卡诺拉蛋白通过膜,渗透物进行第二选择性膜技术以保留2S卡诺拉蛋白并允许低分子量污染物 通过膜,并将来自后一膜技术的滞留物干燥。
    • 9. 发明申请
    • PRODUCTION OF CANOLA PROTEIN
    • 生产加拿大蛋白质
    • WO2007003044A1
    • 2007-01-11
    • PCT/CA2006/001084
    • 2006-06-30
    • BURCON NUTRASCIENCE (MB) CORP.GOSNELL, BrandySEGALL, Kevin, I.SCHWEIZER, Martin
    • GOSNELL, BrandySEGALL, Kevin, I.SCHWEIZER, Martin
    • A23J3/14A23J1/14A23J3/16C11B1/10
    • C11B1/10A23J1/006A23J1/14C07K14/415
    • A canola protein isolate having a protein content of at least about 90 wt% ( N x 6.25), preferably at least about 100 wt%, and consisting predominantly of the 2S protein and substantially free from the 7S and 12S proteins is prepared. In one aspect, canola oil seed meal is extracted with aqueous protein solution at an elevated temperature to preferentially extract 2S protein from the meal to produce a canola protein solution containing predominantly 2S protein. The 2S canola protein is recovered as an isolate. In another aspect, canola oil seed meal is initially extracted with water to preferentially extract 7S and 12S canola proteins followed by extraction of the canola oil seed meal with aqueous saline solution to extract 2S protein from the meal. 2S canola protein isolate is recovered from the saline extract. In another aspect, the canola oil seed meal is extracted with aqueous saline solution to extract 2S, 7S and 12S proteins from the meal. The aqueous protein extract solution is heat treated at an elevated temperature to precipitate 7S and 12S proteins and leave a 2S protein solution from which the isolate may be recovered. In a further aspect, the aqueous protein solution is concentrated prior to the heat treatment.
    • 制备具有至少约90重量%(N x 6.25),优选至少约100重量%的蛋白质含量并主要由2S蛋白质组成且基本上不含7S和12S蛋白质的卡诺拉蛋白分离物。 一方面,在升高的温度下用蛋白质水溶液提取菜籽油籽粉,以优先从膳食中提取2S蛋白,以产生主要含有2S蛋白质的卡诺拉蛋白溶液。 作为分离物回收2S卡诺拉蛋白。 另一方面,最初用水提取油菜籽油籽粉,以优先提取7S和12S卡诺拉蛋白,然后用盐水溶液提取卡诺拉油种子粉,从膳食中提取2S蛋白。 从盐水提取物中回收2S卡诺拉蛋白分离物。 另一方面,用盐水溶液提取卡诺拉籽油籽粉,从膳食中提取2S,7S和12S蛋白质。 将蛋白质水溶液溶液在升高的温度下进行热处理以沉淀7S和12S蛋白质,并留下可从中回收分离物的2S蛋白溶液。 在另一方面,在热处理之前将蛋白质水溶液浓缩。