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1. 发明申请

WO2010111452A1 ISOELECTRIC FOCUSING TRAY AND ELECTRODE ASSEMBLY FOR ALTERNATE GEL STRIP ORIENTATIONS 审中-公开
标题翻译：用于替代凝胶条方向的电光聚焦托盘和电极组件
公开(公告)号：WO2010111452A1
公开(公告)日：2010-09-30
申请号：PCT/US2010/028595
申请日：2010-03-25
申请人： BIO-RAD LABORATORIES, INC. , PEREZ, Evelio
发明人： PEREZ, Evelio
IPC分类号： C07K1/28
CPC分类号： C07K1/28 , G01N27/44782 , G01N27/44795
摘要： The procedures involved in isoelectric focusing that entail the use of an electric current can be performed with the gel side of the IPG strip facing either up or down in a tray and electrode assembly in which the electrodes are mounted on an insert from which the inner ends of the electrodes extend into the troughs to reside either below the IPG strips or above them. Slots in one part (either the insert or the tray) mate with walls or partitions in the other part to secure the two parts together, and the heights of the electrodes within the troughs of the tray can be adjusted by the height of the insert in the tray or by a resilient mounting of the electrode to the insert.
摘要翻译：涉及使用电流的等电聚焦所涉及的步骤可以在IPG带的凝胶侧在托盘和电极组件中向上或向下进行，其中电极安装在插入件上，内端的电极延伸到槽中以位于IPG带之下或其上方。一个部分（插入件或托盘）中的插槽与另一部分中的墙壁或隔板配合，以将两个部件固定在一起，并且托盘槽内电极的高度可以通过插入件的高度进行调整托盘或通过将电极弹性安装到插入件上。

2. 发明申请

WO2008034573A1 COMPOSITIONS AND DEVICES FOR ELECTRO-FILTRATION OF MOLECULES 审中-公开
标题翻译：用于电离过滤分子的组合物和装置
公开(公告)号：WO2008034573A1
公开(公告)日：2008-03-27
申请号：PCT/EP2007/008074
申请日：2007-09-17
申请人： NOVARTIS AG , FAUPEL, Michel, Daniel , VAN OOSTRUM, Jan VAN
发明人： FAUPEL, Michel, Daniel , VAN OOSTRUM, Jan VAN
IPC分类号： B01D71/82 , B01D67/00 , B01D57/02 , C07K1/28 , C12N15/10 , G01N27/447
CPC分类号： B01D61/425 , B01D67/0093 , B01D71/82 , C07K1/28 , G01N27/44747 , G01N27/44795
摘要： The present invention relates to means and devices for electro-filtration of molecules. The membranes comprise N-acryloyl-tris(hydroxymethyl)aminomethane (NAT) covalently linked to a support. The invention further encompasses compositions comprising an isoelectric buffer covalently bound to N-acryloyl-tris(hydroxymethyl)aminomethane (NAT). In particular, the present invention relates to membranes and devices allowing an isoelectric filtration of molecules in solution.
摘要翻译：本发明涉及分子电过滤的方法和装置。膜包含与载体共价连接的N-丙烯酰基 - 三（羟甲基）氨基甲烷（NAT）。本发明还包括包含与N-丙烯酰基 - 三（羟甲基）氨基甲烷（NAT）共价结合的等电子缓冲剂的组合物。特别地，本发明涉及允许溶液中分子的等电过滤的膜和装置。

3. 发明申请

WO02070111A8 METHOD FOR THE MANUFACTURE OF GRADIENT GELS AND METHOD FOR ANALYSING BIOMOLECULES ON ULTRA-LONG IPG GELS 审中-公开
标题翻译：梯度凝胶的制备方法和分析超声乳胶凝胶生物分子的方法
公开(公告)号：WO02070111A8
公开(公告)日：2004-04-29
申请号：PCT/DK0200149
申请日：2002-03-07
申请人： UNIV SYDDANSK , LARSEN PETER MOSE , FEY STEPHEN J
发明人： LARSEN PETER MOSE , FEY STEPHEN J
IPC分类号： B01D15/00 , B01J20/32 , C07K1/28 , G01N27/447 , B01D57/02
CPC分类号： G01N27/44795 , B01D15/00 , B01J20/289 , B01J20/321 , B01J20/3212 , B01J20/3248 , B01J20/327 , B01J20/3272 , B01J20/3274 , B01J20/3282 , B01J20/3285 , B01J20/3297 , C07K1/28 , G01N27/44743
摘要： The invention provides a method for the manufacture of gradient gels, in particular IPG gels, by use of a very dense gel precursor solution, i.e. casting solutions having a density of at least 1.15 kg/L, as well as the polyacrylamide gels as such. The invention also provides a electrophoresis chamber in which a drum carrying a pH-gradient strip is arranged. Furthermore, the invention provides a method of separating a protein mixture in which a moderate voltage gradient is applied.
摘要翻译：本发明提供了通过使用非常致密的凝胶前体溶液，即具有至少1.15kg / L密度的浇铸溶液以及聚丙烯酰胺凝胶制造梯度凝胶，特别是IPG凝胶的方法。本发明还提供一种电泳室，其中布置有带有pH梯度条的鼓。此外，本发明提供了分离其中施加中等电压梯度的蛋白质混合物的方法。

4. 发明申请

WO01036449A1 MULTI-COMPARTMENT ELECTROPHORESIS 审中-公开
标题翻译：多层电泳
公开(公告)号：WO01036449A1
公开(公告)日：2001-05-25
申请号：PCT/AU2000/001391
申请日：2000-11-14
IPC分类号： G01N33/483 , B01D57/02 , C07K1/26 , C07K1/28 , G01N1/10 , G01N27/447 , B01D61/46
CPC分类号： G01N27/44795 , B01D57/02 , C07K1/26 , G01N27/44708
摘要： A novel multi-compartment electrolyser design is used to prefractionate complex protein mixtures, for use prior to the implementation of 2-D maps. Such sub-fractionation processes can effectively remove, via suitable narrow range isoelectric membranes, proteins present in large excess in a cell lysate or in body fluids. In turn, the remaining protein mixture, devoid of such major components, can be loaded in a 2-D map at much higher levels, thus ensuring a greater sensitivity and detection capability of low-abundance proteins. The apparatus is a multi-compartment electrolyser, which is characterised in that recirculation of the samples is not required. The apparatus produces protein fractions which are fully compatible with the subsequent 2-D protocols, since it is based on a focusing technique, which yields samples highly concentrated and devoid of salts and buffers.
摘要翻译：一种新颖的多隔室电解槽设计用于对复杂的蛋白质混合物进行初步分解，以便在实施2-D图之前使用。这样的亚分离方法可以通过合适的窄范围等电聚合物有效地去除在细胞裂解物或体液中大量过量存在的蛋白质。反过来，缺乏这种主要组分的剩余蛋白质混合物可以以更高的水平加载到2-D图中，从而确保低丰度蛋白质的更高的灵敏度和检测能力。该装置是一种多室电解槽，其特征在于不需要再循环样品。该装置产生与随后的2-D方案完全相容的蛋白质级分，因为它基于聚焦技术，其产生高度浓缩且没有盐和缓冲液的样品。

5. 发明申请

WO2018102411A1 METHODS AND APPARATUS FOR SIMULTANEOUSLY DETECTING A LARGE RANGE OF PROTEIN CONCENTRATIONS 审中-公开
公开(公告)号：WO2018102411A1
公开(公告)日：2018-06-07
申请号：PCT/US2017/063704
申请日：2017-11-29
申请人： PROTEINSIMPLE
发明人： XU, Hui , SWARTZ, Kenneth
IPC分类号： B01D15/00 , C07K1/26 , C07K1/28 , G01N1/20 , G01N27/00 , G01N27/447
摘要： Some embodiments described herein relate to a method that includes separating an analyte- containing sample via electrophoresis in a capillary. The capillary is loaded with a chemiluminescence agent, such as luminol, that is configured to react with the analyte (e.g., HRP- conjugated proteins) to produce a signal indicative of a concentration and/or quantity of analyte at each location along the length of the capillary. A first image of the capillary containing the analytes and the chemiluminescence agent is captured over a first period of time. A second image of the capillary containing the analytes and the chemiluminescence agent is captured over a second, longer, period of time. A concentration and/or quantity of a first population of analytes at a first location is determined using the first image, and a concentration and/or quantity of a second population of analytes at a second location is determined using the second image.

6. 发明申请

WO2014028173A1 METHODS FOR PROTEIN PURIFICATION AND ANALYSIS 审中-公开
标题翻译：蛋白质纯化和分析方法
公开(公告)号：WO2014028173A1
公开(公告)日：2014-02-20
申请号：PCT/US2013/051241
申请日：2013-07-19
申请人： ARRAY BRIDGE INC. , WANG, Xing
发明人： WANG, Xing
IPC分类号： C07K1/14 , C07K1/36 , C07K1/28
CPC分类号： C12Q1/485 , C12Q1/26
摘要： Methods to separate complex protein mixtures into individual proteins while maintaining protein function or enzyme activity are disclosed. They are designed to provide high resolution and efficient recovery of the functional proteins. The purified proteins may be analyzed with functional assays including enzymatic activities.
摘要翻译：公开了将复合蛋白混合物分离成单个蛋白质同时保持蛋白质功能或酶活性的方法。它们旨在提供功能蛋白的高分辨率和高效回收。可以用包括酶活性的功能测定来分析纯化的蛋白质。

7. 发明申请

WO2013006138A1 ELECTRODE AND SYSTEM FOR INDUSTRIAL SCALE MEMBRANE BASED FREE-FLOW ISOELECTRIC FOCUSING 审中-公开
标题翻译：基于工业规模膜的电极和系统基于自由流动的电磁聚焦
公开(公告)号：WO2013006138A1
公开(公告)日：2013-01-10
申请号：PCT/SG2012/000185
申请日：2012-05-24
申请人： JOYCE RIVER HI-TECH PTE LTD , CHENG, Jiuhua, Monica
发明人： CHENG, Jiuhua, Monica
IPC分类号： C07K1/28 , B01D57/02
CPC分类号： G01N27/44795 , C07K1/28 , G01N27/44769
摘要： An electrode for Free-Flow Isoelectric Focusing (FFIEF) of water soluble charged molecules and particles comprising a cathode and an anode coated with a metal protective layer and a polymer protective layer. For industrial scale FFIEF used to produce high flux, high selectivity bio-macromolecule products preferably at low cost.
摘要翻译：用于水溶性带电分子的自由流等电聚焦（FFIEF）的电极和包含金属保护层和聚合物保护层的阴极和阳极的颗粒。对于工业规模的FFIEF，用于生产高通量，高选择性的生物大分子产品，优选以低成本。

8. 发明申请

WO2004103519A3 EXTERNAL GRADIENT CHROMATOFOCUSING 审中-公开
标题翻译：外部梯度色谱法
公开(公告)号：WO2004103519A3
公开(公告)日：2005-02-03
申请号：PCT/US2004015216
申请日：2004-05-14
申请人： DNAVAX INC , TSONEV LATCHEZAR I
发明人： TSONEV LATCHEZAR I
IPC分类号： B01D15/16 , B01D15/36 , C07K1/16 , C07K1/18 , C07K1/28 , G01N30/02 , B01D15/08 , G01N30/60
CPC分类号： B01D15/168 , B01D15/362 , B01D15/363 , B01D15/426 , C07K1/16 , C07K1/18 , C07K1/26 , C07K1/28 , G01N30/02
摘要： The invention provides novel methods for the separation of charged molecules such a proteins according to their isoelectric points (pI's) and includes the systems and buffering compositions employed for isolating charged molecules. The invention further provides for modifications to the above described chromatographic methods that enable the separation of charged molecules exhibiting virtually identical pl's by shifting both the buffer's pKa and the pl's of the eluted charged molecules while they are traversing the ion exchange column.
摘要翻译：本发明提供了用于根据其等电点（pI）分离带电荷分子如蛋白质的新方法，并且包括用于分离带电分子的系统和缓冲组合物。本发明进一步提供对上述色谱方法的修改，其能够通过在洗涤的带电分子穿过离子交换柱的同时移动缓冲液的pKa和洗脱的带电分子的P1来分离表现出实质上相同的pl的带电分子。

9. 发明申请

WO2004003564A2 TUMOUR MARKER AND THE USE THEREOF FOR THE DIAGNOSIS AND TREATMENT OF TUMOUR DISEASES 审中-公开
标题翻译：肿瘤标志物及其用途的诊断和肿瘤疾病治疗
公开(公告)号：WO2004003564A2
公开(公告)日：2004-01-08
申请号：PCT/EP0306748
申请日：2003-06-26
申请人： EUROPROTEOME AG , LAMER STEPHANIE , FOGERON MARIE-LAURE , LAGE HERMANN , KELLNER UDO
发明人： LAMER STEPHANIE , FOGERON MARIE-LAURE , LAGE HERMANN , KELLNER UDO
IPC分类号： A61K38/00 , C07K1/28 , G01N33/68 , C07K14/435
CPC分类号： C07K1/28 , A61K38/00
摘要： The invention relates to the use of the type II membrane protein NP 055070 (respectively Swiss-Prot number), NHP2-type protein 1 P55769, pre-mRNA cleavage factor Im (25kD) NP008937, Lysyl-tRNA synthetase Q15046, UNR-interacting protein Q9Y3F4, nucleic transport factor 2 P13662, erythrocyte phosphatase1 isoenzyme F P24666, Prefoldin sub-unit 2 Q9UHV9, heterogeneous nuclear ribonucleoprotein C1/C2 P07910, transitional endoplasmic reticulum-ATPase P55072, bifunctional methylenetetrahydrofolic acid dehydrogenase P13995, 47 kDa heat-shock protein precursor P29043, ubiquinol-cytochrome C reductase complex core protein P31930, the fragments thereof, detector substances targeting the same, and/or nucleic acids coding for the same, for producing a means for diagnosing tumours and for the prophylactic or therapeutic treatment of tumours.
摘要翻译：它是，UNR相互作用蛋白Q9Y3F4使用II型膜蛋白NP 055070（二者SWISS-PROT。号），NHP 2样蛋白1 P55769，前mRNA裂解因子IM（25kD的）NP008937，赖氨酰tRNA合成酶Q15046的，核运输因子2 P13662，Erytrozytenphosphatase1同工酶˚FP24666，Prefoldin亚基2 Q9UHV9，不均一核核糖核蛋白C1 / C2 P07910，transitionale内质网ATP酶P55072，双功能Methylenetetrahydrofolsäure脱氢酶P13995，47 kDa热休克蛋白前体P29043，泛醇细胞色素C的Reductasekomplex核心蛋白P31930其针对这些识别物质和/或编码它们的制备用于肿瘤的诊断，预防或治疗性治疗的药剂的核酸片段建议。

10. 发明申请

WO03019172A2 APPARATUS AND METHOD FOR SEPARATING AN ANALYTE 审中-公开
标题翻译：用于分析分析仪的装置和方法
公开(公告)号：WO03019172A2
公开(公告)日：2003-03-06
申请号：PCT/EP0210369
申请日：2002-08-30
申请人： DIAGNOSWISS SA , MICHEL PHILIPPE , REYMOND FREDERIC , ROSSIER JOEL STEPHANE
发明人： MICHEL PHILIPPE , REYMOND FREDERIC , ROSSIER JOEL STEPHANE
IPC分类号： B01D57/02 , B03C5/00 , C07K1/28 , C12M1/34 , C12M1/38 , C12M1/42 , G01N27/447
CPC分类号： G01N27/44795 , G01N27/44773
摘要： The present invention relates to an apparatus and method for the separation and purification of charged and neutral compounds in an analyte solution by electrophoretic means using a plurality of compartments (1) to contain the analyte solution, an immobilized chemical buffering system (2) to fix a pH value or a pH gradient in its portion(s) contacting the analyte solution and an electrical field imposed by electrical means (4) disposed in at least two of said compartments. This invention allows one to differentially separate charged and neutral compounds, to extract the migrating compounds from the chemical buffering system (2), to recover the purified compounds mainly in solution and to collect them in the various compartments (4), preferably at various pI.
摘要翻译：本发明涉及一种用分离和纯化分析物溶液中的带电荷和中性化合物的装置和方法，该电泳和中性化合物通过使用多个隔室（1）以包含分析物溶液的电泳装置，固定的化学缓冲系统（2）固定在其与分析物溶液接触的部分中的pH值或pH梯度以及由设置在所述隔室中的至少两个中的电气装置（4）施加的电场。本发明允许差异地分离带电和中性化合物，以从化学缓冲系统（2）中提取迁移的化合物，以主要在溶液中回收纯化的化合物并将其收集在各个室（4）中，优选以各种pI 。

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