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    • 1. 发明申请
    • CANDLES AND CANDLE HOLDERS
    • 蜡烛和烛台
    • WO2009047293A1
    • 2009-04-16
    • PCT/EP2008/063538
    • 2008-10-09
    • BARRETT, MichaelMURPHY, Michael
    • BARRETT, MichaelMURPHY, Michael
    • F21S13/12F21V35/00F23D3/16A47G33/00
    • F21V35/00A47G33/00F21S13/12F23D3/16
    • A candle holder (10) comprising a body (11) having a mouth (12), a base (14) and sides (16) that connect the mouth (12) to the base (14) is provided. The mouth (12) comprises an opening (13) arranged to receive a candle and at least a portion of the sides (16) taper inwardly from the mouth (12) towards the base (14). The inward most point of the tapered portion (16c) of the sides (16) defines an aperture (15) having a size which is less than that of the widest part of the candle. Also provided are a candle (20) and a candle stand (40) having a catching means (48) for use with the candle holder (10). In use, wax and waste material from the candle (20) pass through the aperture (15) of the candle holder (10) into the catching means (48). Thus, the candle holder (10) can be reused repeatedly without the need for cleaning or replacement of the candle holder (10). The catching means (48) may be filled with a non-flammable liquid and may be provided with a netted tray (52) for removal of solidified wax and other waste material from the catching means (48).
    • 提供一种包括具有口(12)的本体(11),将口(12)连接到基座(14)的底座(14)和侧面(16))的烛台(10)。 口(12)包括布置成容纳蜡烛的开口(13),并且侧面(16)的至少一部分从嘴部(12)朝向基部(14)向内逐渐变细。 侧面(16)的锥形部分(16c)的最内侧的点限定了具有小于蜡烛最宽部分的尺寸的孔(15)。 还提供了具有用于与烛台(10)一起使用的捕捉装置(48)的蜡烛(20)和蜡烛台(40)。 在使用中,来自蜡烛(20)的蜡和废料通过烛台(10)的孔(15)进入捕获装置(48)。 因此,可以重复使用烛台(10),而不需要清洁或更换烛台(10)。 捕获装置(48)可以填充有非易燃液体,并且可以设置有用于从捕获装置(48)去除固化的蜡和其它废物的网状托盘(52)。
    • 5. 发明申请
    • METHOD AND KIT USED IN PERFORMING HIGH RESOLUTION GENOMIC PROFILING OF CLONAL CELL POPULATIONS IN HETEROGENEOUS TISSUES
    • 在异位组织中克隆细胞群体高分辨率基因分型中使用的方法和试剂盒
    • WO2009055366A1
    • 2009-04-30
    • PCT/US2008/080606
    • 2008-10-21
    • TRANSLATIONAL GENOMICS RESEARCH INSTITUTE (TGEN)BARRETT, Michael
    • BARRETT, Michael
    • C12Q1/68
    • C12Q1/6806C12Q1/6809C12Q2537/16C12Q2539/115C12Q2565/501C12Q2565/626
    • Methods of assessing copy number aberration in a clonal population of cells are disclosed. The method includes purifying nuclei from cells of interest using fluorescence activated cell sorting, amplifying DNA from the nuclei with a highly processive DNA polymerase such as phi29, treating the DNA with an enzyme that catalyzes breaks in DNA such as a DNAse or restriction endonuclease, labeling the DNA with a fluorescent label, and hybridizing the DNA to a microarray capable of performing array comparative genomic hybridization. Cells of interest include tumor cells and may be derived from formalin fixed paraffin embedded tissue. Parameters for isolating nuclei include DNA content and cell cycle state and nuclei may be isolated by centrifugation or other mechanical dissociation in the presence or absence of fluorescent label. Control DNA may be included and labeled with a fluorescent label other than that of the DNA molecule from the cell of interest.
    • 公开了评估细胞克隆群体中拷贝数差异的方法。 该方法包括使用荧光激活的细胞分选来纯化来自细胞的细胞核,用高度进行性的DNA聚合酶例如phi29从细胞核扩增DNA,用催化DNA DNA或限制性内切核酸酶的标记的酶处理DNA 具有荧光标记的DNA,并将DNA与能够进行阵列比较基因组杂交的微阵列杂交。 感兴趣的细胞包括肿瘤细胞,并且可以衍生自福尔马林固定石蜡包埋的组织。 用于分离核的参数包括DNA含量和细胞周期状态,并且可以在存在或不存在荧光标记的情况下通过离心或其它机械解离来分离细胞核。 可以包含对照DNA并用来自感兴趣细胞的DNA分子的荧光标记进行标记。