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    • 5. 发明申请
    • チオールプロテアーゼD3の製造方法
    • 生产THIOL PROTEASE D3的方法
    • WO2002081708A1
    • 2002-10-17
    • PCT/JP2002/003337
    • 2002-04-03
    • 味の素株式会社中村 奈巳小寺 智博丹尾 式希
    • 中村 奈巳小寺 智博丹尾 式希
    • C12N15/57
    • C12N9/50
    • It is intended to quicken autolysis without lowering the activity of thiol protease D3 to thereby shorten the activation treatment time. A process for producing matured thiol protease D3 or its analog characterized by: i) setting DELTA proD3, which has a sequence derived from the N-terminal prosequence of proD3 containing the full N-terminal prosequence of thiol protease D3 by deletion of a part thereof, or its analog under acidic conditions; and ii) recovering a protein having a protease activity which is the product obtained in the step i). Moreover, DELTA proD3 having a sequence derived from the prosequence of D3 by deletion of a part thereof and its analog which are suitable for the above process, a nucleic acid molecule encoding the same, and a recombinant nucleic acid molecule containing the above nucleic acid molecule, in particular a recombinant nucleic acid molecule capable of expressing DELTA proD3 in an appropriate host are provided.
    • 旨在加速自溶而不降低巯基蛋白酶D3的活性,从而缩短活化处理时间。 一种生产成熟硫醇蛋白酶D3或其类似物的方法,其特征在于:i)设定DELTA proD3,其具有源自含有硫羟蛋白酶D3的全N末端序列的proD3的N末端序列的序列,其缺失部分 ,或其在酸性条件下的类似物; 和ii)回收具有蛋白酶活性的蛋白质,其是在步骤i)中获得的产物。 此外,具有衍生自D3的前导序列的序列的DELTA proD3及其部分的缺失及其适用于上述方法的类似物,编码该序列的核酸分子和含有上述核酸分子的重组核酸分子 提供了能够在适当宿主中表达DELTA proD3的重组核酸分子。