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1. 发明申请

WO1996006861A1 TREFOIL PEPTIDE DIMER 审中-公开
公开(公告)号：WO1996006861A1
公开(公告)日：1996-03-07
申请号：PCT/DK1995000343
申请日：1995-08-25
申请人： NOVO NORDISK A/S , THIM, Lars , WÖLDIKE, Helle, Fabricius , NIELSEN, Per, Franklin
发明人： NOVO NORDISK A/S
IPC分类号： C07K14/575
CPC分类号： C07K14/575 , A61K38/00
摘要： A trefoil peptide containing a single trefoil domain characterized by being in dimer form.
摘要翻译：含有单三叶形结构域的三叶肽，其特征在于二聚体形式。

2. 发明申请

WO2012010553A1 N-TERMINAL MODIFIED FGF21 COMPOUNDS 审中-公开
标题翻译： N-末端修饰的FGF21化合物
公开(公告)号：WO2012010553A1
公开(公告)日：2012-01-26
申请号：PCT/EP2011/062238
申请日：2011-07-18
申请人： NOVO NORDISK A/S , TAGMOSE, Tina Møller , GARIBAY, Patrick William , ZHANG, Xujia , THØGERSEN, Henning , NIELSEN, Peter Kresten , ANDERSEN, Birgitte , WANG, Jishu , BAK-JENSEN, Kristian Sass , WÖLDIKE, Helle Fabricius
发明人： TAGMOSE, Tina Møller , GARIBAY, Patrick William , ZHANG, Xujia , THØGERSEN, Henning , NIELSEN, Peter Kresten , ANDERSEN, Birgitte , WANG, Jishu , BAK-JENSEN, Kristian Sass , WÖLDIKE, Helle Fabricius
IPC分类号： A61K38/18
CPC分类号： A61K47/48038 , A61K38/1825 , A61K47/542 , A61K47/545
摘要： Analogues of FGF21 carrying a modifying, substituted ethylene or benzyl group can be used for treatment or prevention of diabetes, dyslipidemia, obesity, cardiovascular diseases, metabolic syndrome, and/or Non Alcoholic Fatty Liver Disease (NAFLD).
摘要翻译：携带改性取代的乙烯或苄基的FGF21的类似物可用于治疗或预防糖尿病，血脂异常，肥胖，心血管疾病，代谢综合征和/或非酒精性脂肪肝（NAFLD）。

3. 发明申请

WO1997000316A1 A PROCESS FOR PRODUCING TRYPSIN (TRYPSINOGEN) 审中-公开
标题翻译：生产TRYPSIN（TRYPSINOGEN）的方法
公开(公告)号：WO1997000316A1
公开(公告)日：1997-01-03
申请号：PCT/DK1996000253
申请日：1996-06-10
申请人： NOVO NORDISK A/S , WÖLDIKE, Helle, Fabricius , KJELDSEN, Thomas, Borglum
发明人： NOVO NORDISK A/S
IPC分类号： C12N09/76
CPC分类号： C12N9/6427 , C12N15/80
摘要： Trypsin (trypsinogen) may be produced in a filamentous fungus by transforming a filamentous fungus with a vector comprising a DNA sequence encoding protrypsin or a derivative thereof N-terminally fused to a DNA sequence encoding a signal peptide, culturing the transformed filamentous fungus in a suitable culture medium to produce trypsinogen and recovering trypsinogen and/or trypsin from the medium.
摘要翻译：胰蛋白酶（胰蛋白酶原）可以通过用包含编码早胰蛋白酶的DNA序列或其衍生物的载体转化丝状真菌来生产丝状真菌，N-末端融合到编码信号肽的DNA序列上，以合适的方式培养转化的丝状真菌培养基以产生胰蛋白酶原并从培养基中回收胰蛋白酶原和/或胰蛋白酶。

4. 发明申请

WO2003080660A3 METHOD FOR THE PREPARATION OF RECOMBINANT MAMMALIAN HEPARIN-BINDING PROTEIN (HBP) 审中-公开
公开(公告)号：WO2003080660A3
公开(公告)日：2003-10-02
申请号：PCT/DK2003/000207
申请日：2003-03-26
申请人： LEUKOTECH A/S , WÖLDIKE, Helle, Fabricius
发明人： WÖLDIKE, Helle, Fabricius
IPC分类号： C07K14/47
摘要： The present invention relates to methods of making heparin-binding protein (HBP) in a recombinant bacterial expression system. In particular, this invention relates to a method for the preparation of an insoluble fusion protein comprising heparin-binding protein (HBP), a proteolytic cleavage- site, and a second polypeptide in recombinant bacterial cells, said fusion protein being accumulated in inclusion bodies in the cytoplasm of bacterium after expression. The invention further relates to methods of separation of the expressed HBP from inclusion bodies comprising purification of HBP from the second polypeptide and optionally refolding of said HBP. The invention further features the DNA constructs comprising different HBP-fusion proteins. Invention also relates to use of the HBP-fusion protein produced in bacteria for the production of pure HBP and use of the HBP purified from the fusion protein for the preparation of a medicament.

5. 发明申请

WO2003080660A2 METHOD FOR THE PREPARATION OF RECOMBINANT MAMMALIAN HEPARIN-BINDING PROTEIN (HBP) 审中-公开
标题翻译：制备重组人血浆蛋白结合蛋白（HBP）的方法
公开(公告)号：WO2003080660A2
公开(公告)日：2003-10-02
申请号：PCT/DK2003/000207
申请日：2003-03-26
申请人： LEUKOTECH A/S , WÖLDIKE, Helle, Fabricius
发明人： WÖLDIKE, Helle, Fabricius
IPC分类号： C07K14/47
CPC分类号： C07K14/4723 , C07K14/47 , C07K2319/20 , C07K2319/21 , C07K2319/95 , C12N15/62
摘要： The present invention relates to methods of making heparin-binding protein (HBP) in a recombinant bacterial expression system. In particular, this invention relates to a method for the preparation of an insoluble fusion protein comprising heparin-binding protein (HBP), a proteolytic cleavage- site, and a second polypeptide in recombinant bacterial cells, said fusion protein being accumulated in inclusion bodies in the cytoplasm of bacterium after expression. The invention further relates to methods of separation of the expressed HBP from inclusion bodies comprising purification of HBP from the second polypeptide and optionally refolding of said HBP. The invention further features the DNA constructs comprising different HBP-fusion proteins. Invention also relates to use of the HBP-fusion protein produced in bacteria for the production of pure HBP and use of the HBP purified from the fusion protein for the preparation of a medicament.
摘要翻译：本发明涉及在重组细菌表达系统中制备肝素结合蛋白（HBP）的方法。特别地，本发明涉及在重组细菌细胞中制备包含肝素结合蛋白（HBP），蛋白水解切割位点和第二多肽的不溶性融合蛋白的方法，所述融合蛋白被积累在包含体内表达后细菌的细胞质。本发明还涉及从包含体分离表达的HBP的方法，包括从第二多肽纯化HBP并任选地重新折叠所述HBP。本发明还涉及包含不同HBP融合蛋白的DNA构建体。本发明还涉及在细菌中产生的HBP-融合蛋白用于生产纯HBP和使用从融合蛋白纯化的HBP用于制备药物的用途。

6. 发明申请

WO1991017244A1 AN ENZYME CAPABLE OF DEGRADING CELLULOSE OR HEMICELLULOSE 审中-公开
标题翻译：可降解纤维素或细胞纤维素的酶
公开(公告)号：WO1991017244A1
公开(公告)日：1991-11-14
申请号：PCT/DK1991000124
申请日：1991-05-08
申请人： NOVO NORDISK A/S , WÖLDIKE, Helle, Fabricius , HAGEN, Fred , HJORT, Carsten, Mailand , HASTRUP, Sven
发明人： NOVO NORDISK A/S
IPC分类号： C12N09/42
CPC分类号： C12N9/2437 , C12Y302/01004 , C12Y302/01091
摘要： A cellulose- or hemicellulose-degrading enzyme which is derivable from a fungus other than Trichoderma or Phanerochaete, and which comprises a carbohydrate binding domain homologous to a terminal A region of Trichoderma reesei cellulases, which carbohydrate binding domain comprises amino acid sequence ( alpha ) or a subsequence thereof capable of effecting binding of the enzyme to an insoluble cellulosic or hemicellulosic substrate.
摘要翻译：可衍生自木霉属或Phanerochaete以外的真菌的纤维素或半纤维素降解酶，其包含与里氏木霉纤维素酶的末端A区同源的碳水化合物结合结构域，所述碳水化合物结合结构域包含氨基酸序列（α）或其能够实现酶与不溶性纤维素或半纤维素基质的结合的亚序列。

7. 发明申请

WO2010052335A1 E. COLI BL21 STRAIN LACKING A FUNCTIONAL GROUP II CAPSULAR GENE CLUSTER 审中-公开
标题翻译：大肠杆菌BL21菌株缺失功能组II基因簇
公开(公告)号：WO2010052335A1
公开(公告)日：2010-05-14
申请号：PCT/EP2009/064903
申请日：2009-11-10
申请人： NOVO NORDISK A/S , WÖLDIKE, Helle Fabricius , JIANHUI, Deng , XIN, Zhao , YUN, Liu , JING, Su
发明人： WÖLDIKE, Helle Fabricius , JIANHUI, Deng , XIN, Zhao , YUN, Liu , JING, Su
IPC分类号： C07K14/61 , C12N1/16 , C12N1/20
CPC分类号： C12P21/02 , C07K14/245 , C07K14/61 , C12N1/20 , C12N15/70 , C12R1/19
摘要： The present invention relates to a novel non-pathogenic Escherichia coli (E. coli) B BL21 strain comprising a deletion of group Il capsular gene cluster, and the use thereof for the production of peptides.
摘要翻译：本发明涉及包含第II组荚膜基因簇缺失的新型非致病性大肠杆菌（E.coli）B BL21菌株及其生产肽的用途。

8. 发明申请

WO1989001969A1 PROCESS FOR THE PRODUCTION OF PROTEIN PRODUCTS IN ASPERGILLUS AND PROMOTERS FOR USE IN ASPERGILLUS 审中-公开
标题翻译：用于生产ASPERGILLUS中蛋白质产品的方法和用于ASPERGILLUS的促销剂
公开(公告)号：WO1989001969A1
公开(公告)日：1989-03-09
申请号：PCT/DK1988000145
申请日：1988-09-02
申请人： NOVO INDUSTRI A/S , WÖLDIKE, Helle, Fabricius
发明人： NOVO INDUSTRI A/S
IPC分类号： C12N15/00
CPC分类号： C12N9/242 , C07K2319/00 , C07K2319/02 , C12N9/62 , C12N15/80
摘要： A process for expression of a protein product in Aspergillus is disclosed. The process comprises transforming an Aspergillus strain with a vector system comprising DNA-sequences encoding a promoter including upstream activating sequences derived from an A. niger amylase, a suitable marker for selection of transformants, and a DNA-sequence encoding the desired protein product. The process enables industrial production of many different polypeptides and proteins in Aspergillus, preferably A. niger. Examples of such products are chymosin or prochymosin and other rennets, proteases, lipases and amylases. Also disclosed is an effective promoter for expression of a protein in Aspergillus, preferably Aspergillus niger being derived from a gene encoding an A. niger amylase. The A. niger amylases are the neutral and acid stable alpha -amylases and a new amylase not so far described and designated XA amylase. Also disclosed is the novel amylase from A. niger XA amylase.
摘要翻译：公开了在曲霉中表达蛋白质产物的方法。该方法包括用包含编码启动子的DNA序列的载体系统转化曲霉菌菌株，所述启动子包括来自黑曲霉淀粉酶的上游活化序列，用于选择转化体的合适标记物和编码所需蛋白质产物的DNA序列。该方法使得能够在曲霉属中产生许多不同的多肽和蛋白质，优选黑曲霉。这些产品的实例是凝乳酶或凝乳酶原和其他雷诺丝，蛋白酶，脂肪酶和淀粉酶。还公开了用于在曲霉中表达蛋白质的有效启动子，优选来自编码黑曲霉淀粉酶的基因的黑曲霉（Aspergillus niger）。黑曲霉淀粉酶是中性和酸稳定的α-淀粉酶和不是迄今描述的新的淀粉酶，并且被称为XA淀粉酶。还公开了来自黑曲霉XA淀粉酶的新型淀粉酶。

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