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1. 发明申请

WO1998028411A2 ENLARGED CELLULASE COMPOSITIONS FOR USE IN THE TREATMENT OF TEXTILES 审中-公开
标题翻译：用于处理纺织品的增透纤维素组合物
公开(公告)号：WO1998028411A2
公开(公告)日：1998-07-02
申请号：PCT/US1997023336
申请日：1997-12-16
申请人： GENENCOR INTERNATIONAL, INC.
发明人： GENENCOR INTERNATIONAL, INC. , BOWER, Benjamin, S. , CLARKSON, Kathleen, A. , LARENAS, Edmund, A. , WARD, Michael
IPC分类号： C12N09/42
CPC分类号： C12N9/2437 , A23K20/189 , C07K2319/00 , C11D3/38645 , C12Y302/01004 , D06M16/003 , D06M2101/06
摘要： The present invention relates to a modified cellulase protein which is advantageously used in the treatment of textiles. Particularly, a method for treating a cellulose containing fabric is provided comprising the steps of forming an aqueous solution comprising a cellulase composition which differs from a precursor cellulase in that it has been enlarged and contacting the aqueous solution with a cellulose containing fabric for a time and under conditions appropriate to treat the fabric. The enlarged cellulase may comprise a multimeric composition of two or more distinct cellulase units or a single cellulase which has had adhered thereto polymeric or fibrous constituents.
摘要翻译：本发明涉及有利地用于纺织品处理的改性纤维素酶蛋白质。特别地，提供了一种处理含纤维素织物的方法，包括以下步骤：形成含有纤维素酶组合物的水溶液，所述纤维素酶组合物与前体纤维素酶不同之处在于其已经被扩大并且使水溶液与含纤维素的织物接触一段时间，在适合处理织物的条件下。扩大的纤维素酶可以包含两个或多个不同的纤维素酶单元的多聚体组合物或已经粘附到其上的聚合物或纤维组分的单一纤维素酶。

2. 发明申请

WO1997011164A1 PURIFIED MANNANASE FROM BACILLUS AMYLOLIQUEFACIENS AND METHOD OF PREPARATION 审中-公开
标题翻译：来自BACILLUS AMYLOLIQUEFACIENS的纯化曼尼诺斯和制备方法
公开(公告)号：WO1997011164A1
公开(公告)日：1997-03-27
申请号：PCT/US1996014692
申请日：1996-09-13
申请人： GENENCOR INTERNATIONAL, INC.
发明人： GENENCOR INTERNATIONAL, INC. , BODIE, Elizabeth, A. , CUEVAS, William, A. , KANTELINEN, Anne, K.
IPC分类号： C12N09/42
CPC分类号： C12N9/2491 , C12Y302/01025
摘要： According to the present invention, a purified mannanase enzyme obtainable from Bacillus amyloliquefaciens is provided. Preferably, the mannanase of the invention has a molecular weight of about 35,000 when analyzed with the SDS-PAGE method and 20,000 when measured by gel filtration, an isoelectric point (pl) of about 5.2-5.6, a pH optimum of about 4.8-5.2 and a half-life at 80 DEG C of about 45 seconds. In another embodiment of the invention, a method is provided for the preparation of a purified mannanase derived from Bacillus amyloliquefaciens.
摘要翻译：根据本发明，提供了可从解淀粉芽孢杆菌获得的纯化甘露聚糖酶。优选地，当用SDS-PAGE方法分析时，本发明的甘露聚糖酶的分子量为约35,000，当通过凝胶过滤测量时为20,000，等电点（pl）为约5.2-5.6，pH最佳值为约4.8-5.2 80℃的半衰期约为45秒。在本发明的另一个实施方案中，提供了用于制备源自解淀粉芽孢杆菌的纯化甘露聚糖酶的方法。

3. 发明申请

WO1995000635A1 LIQUID DETERGENT COMPOSITIONS CONTAINING CELLULASE AND AMINE 审中-公开
标题翻译：含有纤维素和胺的液体洗涤剂组合物
公开(公告)号：WO1995000635A1
公开(公告)日：1995-01-05
申请号：PCT/US1994006154
申请日：1994-06-02
申请人： THE PROCTER & GAMBLE COMPANY , HERBOTS, Ivan, Maurice, Alfons, Jan , JANSEN, Madeleine, Petronella
发明人： THE PROCTER & GAMBLE COMPANY
IPC分类号： C12N09/42
CPC分类号： C12N9/2437 , C11D1/29 , C11D3/30 , C11D3/38645 , C11D3/38663 , C12Y302/01004
摘要： The present invention is a liquid detergent composition containing anionic surfactant and cellulase. Said composition further comprises hydrophobic amines as cellulase stabilizers.
摘要翻译：本发明是含有阴离子表面活性剂和纤维素酶的液体洗涤剂组合物。所述组合物还包含疏水胺作为纤维素酶稳定剂。

4. 发明申请

WO1992003541A1 A PROCESS FOR HYDROLYZING HEMICELLULOSE BY ENZYMES PRODUCED BY TRICHODERMA REESEI 审中-公开
标题翻译：由TRICHODERMA REESEI生产的酶水解血细胞的方法
公开(公告)号：WO1992003541A1
公开(公告)日：1992-03-05
申请号：PCT/FI1991000265
申请日：1991-08-27
申请人： VALTION TEKNILLINEN TUTKIMUSKESKUS , KANTELINEN, Anne , PERE, Jaakko , POUTANEN, Kaisa , TENKANEN, Maija , VIIKARI, Liisa
发明人： VALTION TEKNILLINEN TUTKIMUSKESKUS
IPC分类号： C12N09/42
CPC分类号： D21C5/005 , C12P19/14
摘要： The invention concerns a process for hydrolyzing hemicelluloses, especially xylan, using an enzyme preparation isolated from Trichoderma reesei, containing an endo- beta -xylanase I (pI 5.5) and an endo- beta -xylanase II (pI 9.0). The invention further concerns the use of this enzyme preparation in the improving of the sheet properties and the bleachability of pulps and for the production of sugars from hemicellulosic substrates.
摘要翻译：本发明涉及使用分离自里氏木霉的酶制剂（含内切β-木聚糖酶I（pI 5.5））和内切-β-木聚糖酶II（pI 9.0）来分解半纤维素，特别是木聚糖的方法。本发明还涉及这种酶制剂在改善纸浆性能和纸浆的可漂白性以及从半纤维素基材生产糖的用途。

5. 发明申请

WO1990001060A1 LOW MOLECULAR WEIGHT XYLANASE GLYCOPROTEIN 审中-公开
标题翻译：低分子量XYLANASE GLYCOPROTEIN
公开(公告)号：WO1990001060A1
公开(公告)日：1990-02-08
申请号：PCT/US1988003334
申请日：1988-09-26
申请人： IOWA STATE UNIVERSITY RESEARCH FOUNDATION, INC.
发明人： IOWA STATE UNIVERSITY RESEARCH FOUNDATION, INC. , VARTAK, Hari, Gopal , KASTAWDE, Kulbhushan, Balwant
IPC分类号： C12N09/42
CPC分类号： C12Y302/01032 , C12N9/2482 , C12Y302/01008
摘要： A low molecular weight endo-xylanase has been isolated from an actinomycete strain of Chainia. The purified enzyme which has been partially sequenced is a glycoprotein with a molecular weight between 5,000 and 9,000, estimated at 6,000 ± 1,000. This small molecule xylanase has specific activity for xylan, selectively converting it to oligosaccharides, the enzyme having no significant activity for cellulose.

6. 发明申请

WO1999057256A1 LAUNDRY DETERGENT AND/OR FABRIC CARE COMPOSITIONS COMPRISING A MODIFIED CELLULASE 审中-公开
标题翻译：洗涤剂洗涤剂和/或织物护理组合物，包含改性纤维素酶
公开(公告)号：WO1999057256A1
公开(公告)日：1999-11-11
申请号：PCT/US1998008903
申请日：1998-05-01
申请人： THE PROCTER & GAMBLE COMPANY , BUSCH, Alfred , BETTIOL, Jean-Luc, Philippe , SMETS, Johan , BOYER, Stanton, Lane
发明人： THE PROCTER & GAMBLE COMPANY
IPC分类号： C12N09/42
CPC分类号： C12N9/2437 , C11D3/38645 , C12Y302/01091
摘要： The present invention relates to a modified enzyme and laundry detergent and/or fabric care compositions comprising this modified enzyme. This modified enzyme comprises a catalytically active amino acid sequence of a cellulolytic enzyme EGI linked to an amino acid sequence comprising a Cellulose Binding Domain (CBD). These compositions provide excellent overall cleaning including stain removal and whitening maintenance, while preventing any negative effect on the fabric. These compositions further provide fabric care, including anti-bobbling, depilling, colour appearance, fabric softeness and fabric anti-wear properties and benefits, while preventing any negative effect on the fabric.
摘要翻译：本发明涉及包含该修饰酶的修饰酶和衣物洗涤剂和/或织物护理组合物。该修饰的酶包含与包含纤维素结合结构域（CBD）的氨基酸序列连接的纤维素分解酶EGI的催化活性氨基酸序列。这些组合物提供优异的整体清洁，包括去污和美白维持，同时防止对织物的任何负面影响。这些组合物进一步提供织物护理，包括抗起球，脱毛，颜色外观，织物柔软度和织物抗磨损性能和益处，同时防止对织物的任何负面影响。

7. 发明申请

WO1998012307A1 CELLULASE VARIANTS 审中-公开
标题翻译：纤维素酶变体
公开(公告)号：WO1998012307A1
公开(公告)日：1998-03-26
申请号：PCT/DK1997000393
申请日：1997-09-17
申请人： NOVO NORDISK A/S , ANDERSEN, Kim, Vilbour , SCHÜLEIN, Martin , CHRISTIANSEN, Lars , DAMGAARD, Bo
发明人： NOVO NORDISK A/S
IPC分类号： C12N09/42
CPC分类号： C11D3/38645 , C12N9/2437 , C12Y302/01004
摘要： A method for improving the properties of a cellulolytic enzyme by amino acid substitution, deletion or insertion, the method comprising the steps of: a) constructing a multiple alignment of at least two amino acid sequences known to have three-dimensional structures similar to endoglucanase V (EGV) from Humicola insolens known from Protein Data Bank entry 4ENG; b) constructing a homology-built three-dimensional structure of the cellulolytic enzyme based on the structure of the EGV; c) identifying amino acid residue positions present in a distance from the substrate binding cleft of not more than 5 ANGSTROM ; d) identifying surface-exposed amino acid residues of the enzyme; e) identifying all charged or potentially charged amino acid residue positions of the enzyme; f) choosing one or more positions wherein the amino acid residue is to be substituted, deleted or where an insertion is to be provided; g) carrying out the substitution, deletion or insertion by using conventional protein engineering techniques; and cellulase variants obtained by the method.
摘要翻译：一种用于通过氨基酸取代，缺失或插入改进纤维素分解酶的性质的方法，所述方法包括以下步骤：a）构建已知具有类似于内切葡聚糖酶V的三维结构的至少两个氨基酸序列的多重比对（EGV）从Humicola insolens从蛋白质数据库入口4ENG知道; b）基于EGV的结构构建纤维素分解酶的同源构建的三维结构; c）确定存在于与基底结合裂缝一定距离的氨基酸残基位置不超过5安培; d）鉴定酶的表面暴露的氨基酸残基; e）鉴定酶的所有带电荷或潜在带电的氨基酸残基位置; f）选择其中氨基酸残基被取代，缺失或提供插入的一个或多个位置; g）使用常规蛋白质工程技术进行取代，缺失或插入; 和通过该方法获得的纤维素酶变体。

8. 发明申请

WO1997011165A2 PROTEASE MODIFIED CELLULASE COMPOSITIONS 审中-公开
标题翻译： PROTEASE改性的纤维素组合物
公开(公告)号：WO1997011165A2
公开(公告)日：1997-03-27
申请号：PCT/US1996014976
申请日：1996-09-18
申请人： GENENCOR INTERNATIONAL, INC.
发明人： GENENCOR INTERNATIONAL, INC. , CLARKSON, Kathleen, A. , LARENAS, Edmund, A. , COLLIER, Katherine, D.
IPC分类号： C12N09/42
CPC分类号： C12N9/2437 , A23K20/189 , C11D3/38645 , C12Y302/01004 , D06P5/158
摘要： A modified cellulase and method of preparing is disclosed which comprises a cellulase composition which is treated with a protease wherein at least one proteolytically stable cellulase is present in the cellulase composition. The disclosed cellulase composition may be used in the treatment of lignocellulosic material, the treatment of animal feed, the treatment of waste water, the treatment of textiles, stonewashing or in laundry detergents.
摘要翻译：公开了一种修饰的纤维素酶和制备方法，其包含纤维素酶组合物，其用纤维素酶组合物中存在至少一种蛋白水解稳定的纤维素酶的蛋白酶处理。所公开的纤维素酶组合物可用于木质纤维素材料的处理，动物饲料的处理，废水的处理，纺织品的处理，石洗或洗衣洗涤剂。

9. 发明申请

WO1996011262A1 AN ENZYME AND ENZYME PREPARATION WITH ENDOGLUCANASE ACTIVITY 审中-公开
标题翻译：使用内切酶活性的酶和酶制备
公开(公告)号：WO1996011262A1
公开(公告)日：1996-04-18
申请号：PCT/DK1995000400
申请日：1995-10-06
申请人： NOVO NORDISK A/S , SCHÜLEIN, Martin , OXENBOLL, Karen, Margrethe , ANDERSEN, Lene, Nonboe , LASSEN, Soren, Flensted , KAUPPINEN, Markus, Sakari , NIELSEN, Jack, Bech
发明人： NOVO NORDISK A/S
IPC分类号： C12N09/42
CPC分类号： D21H17/005 , C11D3/38645 , C12N9/2437 , C12N15/81 , C12Y302/01004 , D06M16/003 , D06P5/158 , D21C5/005 , D21H21/10
摘要： An enzyme composition with substantial cellulolytic, especially endoglucanase, activity at alkaline conditions which is derived from or producible by fungi from the genus Acremonium, provided that the enzyme composition is not immunologically cross-reactive with antibodies raised against endoglucanases (EC 3.2.1.4) from Humicola insolens, DSM 1800, Fusarium oxysporum, DSM 2672, Myceliophthora thermophila, CBS 117.65, or Cephalosporium sp., RYM-202. The invention further relates to a DNA construct comprising a DNA sequence encoding an enzyme exhibiting endoglucanase activity. The enzyme composition and the isolated or cloned and expressed cellulase of the invention may be useful in any industrial process requiring an alkaline cellulase, e.g. for providing localised variation in the colour density of dyed fabric such as stone-washing of denim, for improving the drainage of an aqueous suspension of paper pulp, for the de-inking of recycled paper, in detergent compositions and in fabric softeners.
摘要翻译：在碱性条件下具有显着的纤维素分解，特别是内切葡聚糖酶的酶组合物，其衍生自或者由来自顶孢菌属的真菌产生的活性，条件是所述酶组合物与针对内切葡聚糖酶（EC 3.2.1.4）产生的抗体不具有免疫交叉反应性 Humicola insolens，DSM 1800，尖孢镰刀菌，DSM 2672，嗜热丝孢杆菌，CBS 117.65或头孢菌属，RYM-202。本发明还涉及包含编码表现出内切葡聚糖酶活性的酶的DNA序列的DNA构建体。本发明的酶组合物和分离或克隆和表达的纤维素酶可用于任何需要碱性纤维素酶的工业过程，例如，用于提供染色织物的颜色密度的局部变化，例如牛仔布的石洗，用于改进纸浆的水性悬浮液的排出，用于在再生纸中脱墨，在洗涤剂组合物和织物柔软剂中的排墨。

10. 发明申请

WO1995034644A1 PYRODICTIUM XYLANASE, AMYLASE AND PULLULANASE 审中-公开
标题翻译： UM UM UM UM。。。。。。。。。。。。。。
公开(公告)号：WO1995034644A1
公开(公告)日：1995-12-21
申请号：PCT/DK1995000211
申请日：1995-05-31
申请人： NOVO NORDISK A/S , SJOHOLM, Carsten , ANTRANIKIAN, Garabed
发明人： NOVO NORDISK A/S
IPC分类号： C12N09/42
CPC分类号： C12N9/2411 , C12N9/2408 , C12N9/2451 , C12N9/2457 , C12N9/248 , C12Y302/01008 , C12Y302/01032 , C12Y302/01041 , Y02E50/17 , Y10S435/822
摘要： The invention relates to novel thermostable amylases, pullulanases and xylanases obtainable from Pyrodictium.
摘要翻译：本发明涉及可从Pyrodictium获得的新型热稳定淀粉酶，支链淀粉酶和木聚糖酶。

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