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    • 2. 发明申请
    • A METHOD FOR IMMOBILIZING A POLYPEPTIDE IN A POLYMER AND A MEMBRANE PRODUCED THEREBY
    • 一种在聚合物中固定多肽的方法及其制备的薄膜
    • WO1989007139A1
    • 1989-08-10
    • PCT/DK1989000019
    • 1989-02-03
    • NOVO INDUSTRI A/SEGE, Henrik
    • NOVO INDUSTRI A/S
    • C12N11/08
    • C12Q1/003C12N11/04Y10S435/817
    • The present invention relates generally to the immobilization or incorporation of polypeptides, especially enzymes or other bioactive polypeptides into polymeric matrixes, especially polyurethane, membranes produced by said polymers, and the utilization of such membranes in biosensors. A preferred type of biosensor is the needle sensor designed for in vivo monitoring of glucose which comprises a core platinum anode (2) coated with an insulating lacquer (3), the anode (2) is situated inside a stainless steel reference cathode (4) which is insulated from the anode (2) by a layer of epoxy resin (5). At one end, the tip, the electrode (1) has a detection surface (6), which is in an acute angle to the general direction of the electrode (1). At the other end, the base, the electrode (2) is provided with terminals (7) and (8) for the anode (2) and cathode (4), respectively. The terminals (7) and (8) are soldered to leads (9) and (10), respectively, which are connected to instrumentation used when performing measurements.
    • 本发明一般涉及将多肽,特别是酶或其他生物活性多肽固定或并入聚合物基质,特别是由所述聚合物制备的聚氨酯膜,以及在生物传感器中利用这种膜。 优选的生物传感器类型是设计用于体内监测葡萄糖的针传感器,其包括涂覆有绝缘漆(3)的核心铂阳极(2),阳极(2)位于不锈钢参考阴极(4)内部, 其通过一层环氧树脂(5)与阳极(2)绝缘。 在一端,电极(1)具有与电极(1)的大致方向成锐角的检测面(6)。 在另一端,电极(2)分别设有用于阳极(2)和阴极(4)的端子(7)和(8)。 端子(7)和(8)分别焊接到引线(9)和(10),引线(9)和(10)连接到进行测量时使用的仪器。