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1. 发明申请

WO1996006163A1 NOVEL RHAMNOGALACTURONAN RHAMNOSIDASES 审中-公开
标题翻译：新型RHAMNOGALACTURONAN RHAMNOSIDASES
公开(公告)号：WO1996006163A1
公开(公告)日：1996-02-29
申请号：PCT/DK1995000337
申请日：1995-08-17
申请人： NOVO NORDISK A/S , MUTTER, Margien , SCHOLS, Henk, Arie , BELDMAN, Gerrit , VORAGEN, Alphons, Gerard, Joseph
发明人： NOVO NORDISK A/S
IPC分类号： C12N09/24
CPC分类号： C12Y302/01173 , C12N9/2402
摘要： The present invention relates to hitherto unknown class of enzymes with rhamnogalacturonan rhamnosidase (RG-rhamnosidase) activity as well as the use of such enzymes for the degradation or modification of plant cell wall materials.
摘要翻译：本发明涉及迄今为止未知类型的具有鼠李糖半乳糖醛酸鼠李糖苷酶（RG-鼠李糖苷酶）活性的酶以及这些酶用于降解或修饰植物细胞壁材料的用途。

2. 发明申请

WO1994020612A1 AN ENZYME WITH RHAMNOGALACTURONASE ACTIVITY 审中-公开
标题翻译：具有消炎激酶活性的酶
公开(公告)号：WO1994020612A1
公开(公告)日：1994-09-15
申请号：PCT/DK1994000097
申请日：1994-03-04
申请人： NOVO NORDISK A/S , KOFOD, Lene, Venke , DALBOGE, Henrik , ANDERSEN, Lene, Nonboe , KAUPPINEN, Markus, Sakari , CHRISTGAU, Stephan , HELDT-HANSEN, Hans, Peter , CHRISTOPHERSEN, Claus , NIELSEN, Per, Munk , VORAGEN, Alphons, Gerard, Joseph , SCHOLS, Hendrik, Arie
发明人： NOVO NORDISK A/S
IPC分类号： C12N09/24
CPC分类号： C13B10/083 , A23K20/189 , A23L2/84 , A23L29/06 , C12N9/2402 , C12Y302/01171 , C13K13/00
摘要： An enzyme exhibiting rhamnogalacturonase activity, which enzyme: a) is encoded by the DNA sequence shown in SEQ ID No. 1 or a sequence homologous thereto encoding a polypeptide with RGase activity, b) has the amino acid sequence shown in SEQ ID No. 2 or an analogue thereof, c) is reactive with an antibody raised against the enzyme encoded by the DNA sequence shown in SEQ ID No. 1, d) has a pH optimum above pH 5, and/or e) has a relative activity of at least 30% at a pH in the range of 5.5-6.5. The enzyme may be used for reducing the viscosity of a plant cell wall material and for extracting high molecular weight molecules from a plant cell wall material.
摘要翻译：显示鼠李糖半乳糖醛酸酶活性的酶，其酶：a）由SEQ ID No.1所示的DNA序列或与其同源的序列编码，具有RGase活性的多肽，b）具有SEQ ID No.2所示的氨基酸序列或其类似物，c）与针对由SEQ ID No.1所示的DNA序列编码的酶产生的抗体具有反应性，d）具有优于pH 5以上的pH，和/或e）具有相对活性为在5.5-6.5范围内的pH值至少为30％。该酶可用于降低植物细胞壁材料的粘度并从植物细胞壁材料中提取高分子量分子。

3. 发明申请

WO1998028991A2 CLOUDY FRUIT JUICES AND METHODS FOR MAKING SAME 审中-公开
标题翻译：云南果汁及其制作方法
公开(公告)号：WO1998028991A2
公开(公告)日：1998-07-09
申请号：PCT/EP1997007139
申请日：1997-12-10
申请人： GIST-BROCADES B.V. , VINCKEN, Jean, Paul , BELDMAN, Gerrit , VORAGEN, Alphons, Gerard, Joseph
发明人： GIST-BROCADES B.V.
IPC分类号： A23L02/02
CPC分类号： A23L2/62
摘要： It was surprisingly found that a cloudy apple juice is obtained when a method is used comprising the step of treating apple material with a mixture of enzymes comprising a homogalacturonan depolymerising activity, such as pectin lyase (EC 4.2.2.10), and an enzyme with endoglucanase activity (EC 3.-2.1.4), in the absence of enzymes which reduce the cloud volume by more than 25 %, as determined by a method which comprises: (a) suspending 5 mg of a cloud material obtainable by homogenising 1 g of apple pieces of Malus malus L. cv. Jonagold, after removal of the peel and core, using a Braun Kitchen machine (MX32, Frankfurt, Germany; 5 mm blade), incubating at 40 DEG C under continuous head-over-tail mixing at 150 rpm in 3 mL of a 200 mM NaOAc buffer (pH4) containing 0.01% (w/v) NaN3, 1 % ascorbic acid, 50 mU pectin lyase and an amount of endoglucanase with an equivalent of 25 mU of xyloglucanase activity, in 3 ml of a 25 mM NaOAc buffer (pH 5), containing about 0.01 % (w/v) NaN3, (b) incubating the cloud suspension so obtained for 16 h at about 40 DEG C, in the presence of the said enzyme, during which the cloud suspension and enzyme is mixed head-over-tail, (c) allowing the cloud to settle at about 4 DEG C for 24 hours, and (d) determining the effect of the enzyme on cloud stability by calculating the difference between the cloud volume of the cloud suspension obtained by following the above steps in the absence of the said enzyme and subtracting the volume obtained of the cloud suspension obtained by following the above steps in the presence of the enzyme.
摘要翻译：令人惊奇地发现，当使用包括如下步骤的方法时，获得了混浊的苹果汁，所述方法是用包含均聚半乳糖醛酸解聚活性的酶的混合物（例如果胶裂解酶（EC 4.2.2.10））和具有内切葡聚糖酶活性（EC 3.-2.1.4），如果通过包括以下方法测定的方法测定，则在没有将云体积减少超过25％的酶的情况下，（a）将5mg通过均化1g 苹果黄瓜的苹果 Jonagold使用Braun Kitchen机器（MX32，法兰克福，德国; 5毫米刀片）去除皮和芯后，在连续的头尾混合下以150rpm在3mL的200mM 将含有0.01％（w / v）NaN 3，1％抗坏血酸，50mU果胶裂解酶和一定量的具有25mU木葡聚糖酶活性的相当量的内切葡聚糖酶的3ml 25mM NaOAc缓冲液（pH 5），含有约0.01％（w / v）NaN 3，（b）在所述酶的存在下，将所得到的云悬浮液在约40℃下孵育16小时，在此期间云悬浮液和酶混合头（c）允许云在约4℃下沉降24小时，（d）通过计算通过下列获得的云悬浮云体积之间的差异来确定酶对云稳定性的影响在不存在所述酶的情况下的上述步骤，并减去通过以下获得的获得的云悬浮液的体积在酶的存在下的上述步骤。

4. 发明申请

WO9941386A3 NOVEL ENDO-XYLOGALACTURONASE 审中-公开
标题翻译：新型内切酶活性
公开(公告)号：WO9941386A3
公开(公告)日：2000-01-20
申请号：PCT/EP9900860
申请日：1999-02-09
申请人： DSM NV , MEEUWSEN PETRUS JOHANNES ALBER , VLUGT BERGMANS CECILE JOHANNA , VINCKEN JEAN PAUL , BELDMAN GERRIT , VORAGEN ALPHONS GERARD JOSEPH , HERWEIJER MARGARETA ADRIANA , OOIJEN ALBERT JOHANNES JOSEPH
发明人： MEEUWSEN PETRUS JOHANNES ALBER , VAN DER VLUGT-BERGMANS CECILE , VINCKEN JEAN PAUL , BELDMAN GERRIT , VORAGEN ALPHONS GERARD JOSEPH , HERWEIJER MARGARETA ADRIANA , VAN OOIJEN ALBERT JOHANNES JOS
IPC分类号： A23L2/84 , A23L29/00 , A23L29/231 , C12N9/24 , C12N15/55 , C12P21/00 , C12Q1/34 , A23L1/0524 , A23L1/06
CPC分类号： C12Y302/01171 , A23L2/84 , A23L29/06 , A23L29/231 , C12N9/2402 , C12Q1/34 , C12Y302/01015 , C12Y302/01099 , G01N2333/924
摘要： Polypeptides possessing a novel activity, namely endo-xylogalacturonase activity, are disclosed. These polypeptides can degrade pectin found in plant extracts and plant materials, and in particular the "hairy" regions of pectin polymers. In particular, the polypeptides can cleave in a galacturonic acid polymer at internal glycocidic bonds. The novel enzyme XghA is disclosed, and its amino acid sequence and encoding DNA sequence given. This polypeptide was expressed in yeast cells and has been used to treat vegetable material, in particular soy and fruit juice in the preparation of edible foodstuffs.
摘要翻译：公开了具有新活性的多肽，即内切木糖聚半乳糖醛酸酶活性。这些多肽可以降解在植物提取物和植物材料中发现的果胶，特别是果胶聚合物的“毛发”区域。特别地，多肽可以在内部糖苷键处在半乳糖醛酸聚合物中切割。公开了新型XghA酶，其氨基酸序列和编码DNA序列。该多肽在酵母细胞中表达，并且已经用于处理植物材料，特别是在可食用食品的制备中的大豆和果汁。

5. 发明申请

WO9828991A3 CLOUDY FRUIT JUICES AND METHODS FOR MAKING SAME 审中-公开
标题翻译：云南果汁及其制作方法
公开(公告)号：WO9828991A3
公开(公告)日：1998-11-12
申请号：PCT/EP9707139
申请日：1997-12-10
申请人： GIST BROCADES BV , VINCKEN JEAN PAUL , BELDMAN GERRIT , VORAGEN ALPHONS GERARD JOSEPH
发明人： VINCKEN JEAN PAUL , BELDMAN GERRIT , VORAGEN ALPHONS GERARD JOSEPH
IPC分类号： C12N15/09 , A23L2/62 , A23L2/82 , C12N1/19 , C12N9/42 , C12R1/645 , C12R1/685 , A23L2/02 , A23L2/04
CPC分类号： A23L2/62
摘要： It was surprisingly found that a cloudy apple juice is obtained when a method is used comprising the step of treating apple material with a mixture of enzymes comprising a homogalacturonan depolymerising activity, such as pectin lyase (EC 4.2.2.10), and an enzyme with endoglucanase activity (EC 3.-2.1.4), in the absence of enzymes which reduce the cloud volume by more than 25 %, as determined by a method which comprises: (a) suspending 5 mg of a cloud material obtainable by homogenising 1 g of apple pieces of Malus malus L. cv. Jonagold, after removal of the peel and core, using a Braun Kitchen machine (MX32, Frankfurt, Germany; 5 mm blade), incubating at 40 °C under continuous head-over-tail mixing at 150 rpm in 3 mL of a 200 mM NaOAc buffer (pH4) containing 0.01% (w/v) NaN3, 1 % ascorbic acid, 50 mU pectin lyase and an amount of endoglucanase with an equivalent of 25 mU of xyloglucanase activity, in 3 ml of a 25 mM NaOAc buffer (pH 5), containing about 0.01 % (w/v) NaN3, (b) incubating the cloud suspension so obtained for 16 h at about 40 °C, in the presence of the said enzyme, during which the cloud suspension and enzyme is mixed head-over-tail, (c) allowing the cloud to settle at about 4 °C for 24 hours, and (d) determining the effect of the enzyme on cloud stability by calculating the difference between the cloud volume of the cloud suspension obtained by following the above steps in the absence of the said enzyme and subtracting the volume obtained of the cloud suspension obtained by following the above steps in the presence of the enzyme.

6. 发明申请

WO1994026880A1 COMBINED ACTION OF ENDOGLUCANASES AND CELLOBIOHYDROLASES 审中-公开
标题翻译：内毒素和细胞溶血素的组合作用
公开(公告)号：WO1994026880A1
公开(公告)日：1994-11-24
申请号：PCT/EP1994001531
申请日：1994-05-10
申请人： GIST-BROCADES N.V. , VINCKEN, Jean, Paul , BELDMAN, Gerrit , VORAGEN, Alphons, Gerard, Joseph
发明人： GIST-BROCADES N.V.
IPC分类号： C12N09/24
CPC分类号： C12Y302/01091 , A23K10/14 , A23L2/84 , A23L5/25 , C08B37/0057 , C12N9/2437 , C12Y302/01004 , D06M16/003 , D21C5/005
摘要： The present invention discloses the use of combinations of cell wall degrading enzymes in the degradation of cell wall derived substrates. Specifically, the use of combinations of endoglucanases and cellobiohydrolases, comprising xyloglucanase activity is demonstrated.
摘要翻译：本发明公开了细胞壁降解酶组合在细胞壁衍生底物降解中的应用。具体地说，证明了使用包含木葡聚糖酶活性的内切葡聚糖酶和纤维二糖水解酶的组合。

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