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1. 发明申请

WO1995031533A1 AN ENZYME WITH ENDO-1,3(4)-'beta'-GLUCANASE ACTIVITY 审中-公开
标题翻译：具有内切-1,3（4）-β-GLUCANASE活性的酶
公开(公告)号：WO1995031533A1
公开(公告)日：1995-11-23
申请号：PCT/DK1995000188
申请日：1995-05-11
申请人： NOVO NORDISK A/S , KOFOD, Lene, Venke , ANDERSEN, Lene, Nonboe , KAUPPINEN, Markus, Sakari , CHRISTGAU, Stephan , DALBOGE, Henrik , OLSEN, Hans, Sejr , BREINHOLT, Jens
发明人： NOVO NORDISK A/S
IPC分类号： C12N09/42
CPC分类号： C12Y302/01006 , A21D8/042 , A61K8/66 , A61Q11/00 , C11D3/38645 , C12N9/244 , D06M16/003
摘要： The present invention relates to an enzyme with endo-beta-glucanase activity, encoded by a DNA sequence. Further, the invention relates to a DNA construct encoding the enzyme, a method of producing the enzyme, an enzyme preparation containing the enzyme, the use of said enzyme or said preparation for a number of uses including the degradation or modification of beta-glucan containing materials. Also contemplated is an isolated substantially pure culture of Saccharomyces cerevisiae NN049006, transformed with a DNA sequence derived from Acremonium sp.
摘要翻译：本发明涉及由DNA序列编码的内切β-葡聚糖酶活性的酶。此外，本发明涉及编码该酶的DNA构建体，该酶的制备方法，含该酶的酶制剂，所述酶或所述制剂的用途，包括降解或修饰含有β-葡聚糖的多种用途材料。还考虑了用源自Acremonium sp。的DNA序列转化的酿酒酵母NN049006的分离的基本上纯的培养物。

2. 发明申请

WO1997027293A1 AN ENZYME WITH XYLANASE ACTIVITY 审中-公开
标题翻译：具有XYLANASE活性的酶
公开(公告)号：WO1997027293A1
公开(公告)日：1997-07-31
申请号：PCT/DK1997000033
申请日：1997-01-22
申请人： NOVO NORDISK A/S , SANDAL, Thomas , KOFOD, Lene, Venke , KAUPPINEN, Markus, Sakari , ANDERSEN, Lene, Nonboe
发明人： NOVO NORDISK A/S
IPC分类号： C12N09/24
CPC分类号： C12Y302/01032 , A21D8/042 , A23K20/189 , A23L27/24 , A23L29/06 , C12C5/004 , C12C5/006 , C12C7/00 , C12G1/0203 , C12N9/248 , C12Y302/01008 , C13K13/002 , D21C5/005
摘要： The present invention relates to an enzyme with xylanase activity, a DNA construct encoding the enzyme with xylanase activity, a method of producing the enzyme, an enzyme preparation comprising said enzyme with xylanase activity, a detergent composition comprising said xylanase, and the use of said enzyme and enzyme preparation for a number of industrial applications.
摘要翻译：本发明涉及具有木聚糖酶活性的酶，编码具有木聚糖酶活性的酶的DNA构建体，生产该酶的方法，包含所述酶的木糖酶活性的酶制剂，包含所述木聚糖酶的洗涤剂组合物，以及使用所述酶和酶制剂用于许多工业应用。

3. 发明申请

WO1997027292A1 AN ENZYME WITH XYLANASE ACTIVITY 审中-公开
标题翻译：具有木聚糖酶活性的酶
公开(公告)号：WO1997027292A1
公开(公告)日：1997-07-31
申请号：PCT/DK1997000032
申请日：1997-01-22
申请人： NOVO NORDISK A/S , SANDAL, Thomas , KOFOD, Lene, Venke , KAUPPINEN, Markus, Sakari , ANDERSEN, Lene, Nonboe , DYBDAL, Lone
发明人： NOVO NORDISK A/S
IPC分类号： C12N09/24
CPC分类号： C12Y302/01032 , C12N9/248 , C12R1/865 , C12Y302/01008
摘要： The present invention relates to an enzyme with xylanase activity, a cloned DNA sequence encoding the enzyme with xylanase activity, a method of producing the enzyme, an enzyme preparation comprising said enzyme with xylanase activity, a detergent composition comprising said xylanase, and the use of said enzyme and enzyme preparation for a number of industrial applications.
摘要翻译：本发明涉及具有木聚糖酶活性的酶，编码具有该木聚糖酶活性的酶的克隆DNA序列，该酶的制造方法，含有具有该活性的酶的酶制剂。木聚糖酶类型，包含所述木聚糖酶的洗涤剂组合物，以及该酶和所述酶制剂在许多工业应用中的用途。

4. 发明申请

WO1994020612A1 AN ENZYME WITH RHAMNOGALACTURONASE ACTIVITY 审中-公开
标题翻译：具有消炎激酶活性的酶
公开(公告)号：WO1994020612A1
公开(公告)日：1994-09-15
申请号：PCT/DK1994000097
申请日：1994-03-04
申请人： NOVO NORDISK A/S , KOFOD, Lene, Venke , DALBOGE, Henrik , ANDERSEN, Lene, Nonboe , KAUPPINEN, Markus, Sakari , CHRISTGAU, Stephan , HELDT-HANSEN, Hans, Peter , CHRISTOPHERSEN, Claus , NIELSEN, Per, Munk , VORAGEN, Alphons, Gerard, Joseph , SCHOLS, Hendrik, Arie
发明人： NOVO NORDISK A/S
IPC分类号： C12N09/24
CPC分类号： C13B10/083 , A23K20/189 , A23L2/84 , A23L29/06 , C12N9/2402 , C12Y302/01171 , C13K13/00
摘要： An enzyme exhibiting rhamnogalacturonase activity, which enzyme: a) is encoded by the DNA sequence shown in SEQ ID No. 1 or a sequence homologous thereto encoding a polypeptide with RGase activity, b) has the amino acid sequence shown in SEQ ID No. 2 or an analogue thereof, c) is reactive with an antibody raised against the enzyme encoded by the DNA sequence shown in SEQ ID No. 1, d) has a pH optimum above pH 5, and/or e) has a relative activity of at least 30% at a pH in the range of 5.5-6.5. The enzyme may be used for reducing the viscosity of a plant cell wall material and for extracting high molecular weight molecules from a plant cell wall material.
摘要翻译：显示鼠李糖半乳糖醛酸酶活性的酶，其酶：a）由SEQ ID No.1所示的DNA序列或与其同源的序列编码，具有RGase活性的多肽，b）具有SEQ ID No.2所示的氨基酸序列或其类似物，c）与针对由SEQ ID No.1所示的DNA序列编码的酶产生的抗体具有反应性，d）具有优于pH 5以上的pH，和/或e）具有相对活性为在5.5-6.5范围内的pH值至少为30％。该酶可用于降低植物细胞壁材料的粘度并从植物细胞壁材料中提取高分子量分子。

5. 发明申请

WO1994014952A1 AN ENZYME WITH POLYGALACTURONASE ACTIVITY 审中-公开
标题翻译：具有聚糖酶活性的酶
公开(公告)号：WO1994014952A1
公开(公告)日：1994-07-07
申请号：PCT/DK1993000445
申请日：1993-12-23
申请人： NOVO NORDISK A/S , DALBOEGE, Henrik , ANDERSEN, Lene, Nonboe , KOFOD, Lene, Venke , KAUPPINEN, Markus, Sakari , CHRISTGAU, Stephan , HELDT-HANSEN, Hans, Peter , HALKIER, Torben
发明人： NOVO NORDISK A/S
IPC分类号： C12N09/24
CPC分类号： C12G1/0203 , C12N9/2402 , C12Y302/01015
摘要： An enzyme exhibiting polygalacturonase activity, which enzyme is immunologically reactive with an antibody raised against a purified polygalacturonase derived from Aspergillus aculeatus, CBS 101.43. The enzyme may be produced by recombinant DNA techniques and may be used for degradation of plant cell walls, for instance in the wine and juice production.
摘要翻译：显示多聚半乳糖醛酸酶活性的酶，该酶与针对源自Aspergillus aculeatus的纯化多聚半乳糖醛酸酶CBS 101.43产生的抗体具有免疫反应性。该酶可以通过重组DNA技术产生并且可以用于植物细胞壁的降解，例如在葡萄酒和果汁生产中。

6. 发明申请

WO1996022366A1 TRANSGLUTAMINASES FROM OOMYCETES 审中-公开
标题翻译：来自OOMYCETES的转氨酶
公开(公告)号：WO1996022366A1
公开(公告)日：1996-07-25
申请号：PCT/DK1996000031
申请日：1996-01-19
申请人： NOVO NORDISK A/S , BECH, Lisbeth , RASMUSSEN, Grethe , HALKIER, Torben , OKADA, Mariko , ANDERSEN, Lene, Nonboe , KAUPPINEN, Markus, Sakari , SANDAL, Thomas
发明人： NOVO NORDISK A/S
IPC分类号： C12N09/10
CPC分类号： C12Y203/02013 , A21D8/042 , C12N9/1044 , C14C11/00 , Y10S435/911
摘要： Transglutaminase and transglutaminase preparations can be produced by lower fungi belonging to the class Oomycetes and unprecedented high-level expression is achievable by growing these coenocytium forming organisms, especially the strains Pythium sp., Pythium irregulare, Pythium dissotocum, Phythium periilum (or P. periplocum), Pythium torulosum, Pythium ultimum, Pythium aphanidermatum, Phytophthora cactorum, Phytophthora palmivora, Phytophthora porri, Phytophthora infestans, Phytophthora megasperma, Phytophthora cinnamomi and Phytophthora cryptogea; and a recombinant transglutaminase has been cloned and expressed, the enzyme and enzyme preparations being useful for cross-linking proteins, e.g. in flour, baked products, meat products, fish products, cosmetics, cheese, milk products, gelled food products and leather finishing, or as a glutaminase , e.g. in bread and other baked glutein-containing food products.
摘要翻译：转谷氨酰胺酶和转谷氨酰胺酶制剂可以通过属于卵菌纲的较低真菌生产，并且前所未有的高水平表达是通过生长这些形成细胞的生物体，特别是菌株腐霉菌，非腐霉菌，不孢菌腐霉，腐质腐霉（P. periplocum）），腐霉，腐霉，腐霉，腐皮疫霉，致病疫霉，疫霉疫霉，致热疫霉，疫霉疫霉和疫霉疫霉; 已经克隆和表达重组转谷氨酰胺酶，酶和酶制剂可用于交联蛋白质，例如，在面粉，烘焙食品，肉制品，鱼制品，化妆品，奶酪，乳制品，凝胶食品和皮革整理中，或作为谷氨酰胺酶。在面包和其他含烤溜溜蛋白的食品中。

7. 发明申请

WO1996011262A1 AN ENZYME AND ENZYME PREPARATION WITH ENDOGLUCANASE ACTIVITY 审中-公开
标题翻译：使用内切酶活性的酶和酶制备
公开(公告)号：WO1996011262A1
公开(公告)日：1996-04-18
申请号：PCT/DK1995000400
申请日：1995-10-06
申请人： NOVO NORDISK A/S , SCHÜLEIN, Martin , OXENBOLL, Karen, Margrethe , ANDERSEN, Lene, Nonboe , LASSEN, Soren, Flensted , KAUPPINEN, Markus, Sakari , NIELSEN, Jack, Bech
发明人： NOVO NORDISK A/S
IPC分类号： C12N09/42
CPC分类号： D21H17/005 , C11D3/38645 , C12N9/2437 , C12N15/81 , C12Y302/01004 , D06M16/003 , D06P5/158 , D21C5/005 , D21H21/10
摘要： An enzyme composition with substantial cellulolytic, especially endoglucanase, activity at alkaline conditions which is derived from or producible by fungi from the genus Acremonium, provided that the enzyme composition is not immunologically cross-reactive with antibodies raised against endoglucanases (EC 3.2.1.4) from Humicola insolens, DSM 1800, Fusarium oxysporum, DSM 2672, Myceliophthora thermophila, CBS 117.65, or Cephalosporium sp., RYM-202. The invention further relates to a DNA construct comprising a DNA sequence encoding an enzyme exhibiting endoglucanase activity. The enzyme composition and the isolated or cloned and expressed cellulase of the invention may be useful in any industrial process requiring an alkaline cellulase, e.g. for providing localised variation in the colour density of dyed fabric such as stone-washing of denim, for improving the drainage of an aqueous suspension of paper pulp, for the de-inking of recycled paper, in detergent compositions and in fabric softeners.
摘要翻译：在碱性条件下具有显着的纤维素分解，特别是内切葡聚糖酶的酶组合物，其衍生自或者由来自顶孢菌属的真菌产生的活性，条件是所述酶组合物与针对内切葡聚糖酶（EC 3.2.1.4）产生的抗体不具有免疫交叉反应性 Humicola insolens，DSM 1800，尖孢镰刀菌，DSM 2672，嗜热丝孢杆菌，CBS 117.65或头孢菌属，RYM-202。本发明还涉及包含编码表现出内切葡聚糖酶活性的酶的DNA序列的DNA构建体。本发明的酶组合物和分离或克隆和表达的纤维素酶可用于任何需要碱性纤维素酶的工业过程，例如，用于提供染色织物的颜色密度的局部变化，例如牛仔布的石洗，用于改进纸浆的水性悬浮液的排出，用于在再生纸中脱墨，在洗涤剂组合物和织物柔软剂中的排墨。

8. 发明申请

WO1995031534A1 AN ENZYME WITH 'beta'-(1-6)-ENDOGLUCANASE ACTIVITY 审中-公开
标题翻译：具有β - （1-6）-ENDOGLUCANASE活性的酶
公开(公告)号：WO1995031534A1
公开(公告)日：1995-11-23
申请号：PCT/DK1995000189
申请日：1995-05-11
申请人： NOVO NORDISK A/S , KOFOD, Lene, Venke , ANDERSEN, Lene, Nonboe , KAUPPINEN, Markus, Sakari , CHRISTGAU, Stephan , DALBOGE, Henrik , OLSEN, Hans, Sejr
发明人： NOVO NORDISK A/S
IPC分类号： C12N09/42
CPC分类号： C12Y302/01075 , C12N9/2402 , Y10S435/945
摘要： The present invention relates to an enzyme with endo-beta-glucanase activity, encoded by a DNA sequence. Further, the invention relates to a DNA construct encoding the enzyme, a method of producing the enzyme, an enzyme preparation containing the enzyme, the use of said enzyme or said preparation for a number of uses including the degradation or modification of beta-glucan containing materials.
摘要翻译：本发明涉及由DNA序列编码的内切β-葡聚糖酶活性的酶。此外，本发明涉及编码该酶的DNA构建体，该酶的制备方法，含该酶的酶制剂，所述酶或所述制剂的用途，包括降解或修饰含有β-葡聚糖的多种用途材料。

9. 发明申请

WO1995002689A1 AN ENZYME WITH ACETYL ESTERASE ACTIVITY 审中-公开
标题翻译：具有乙酰酯酶活性的酶
公开(公告)号：WO1995002689A1
公开(公告)日：1995-01-26
申请号：PCT/DK1994000285
申请日：1994-07-13
申请人： NOVO NORDISK A/S , CHRISTGAU, Stephan , SANDAL, Thomas , KAUPPINEN, Markus, Sakari , HALKIER, Torben , DALBOGE, Henrik
发明人： NOVO NORDISK A/S
IPC分类号： C12N09/18
CPC分类号： C12N9/18
摘要： An enzyme with acetyl esterase activity, which enzyme comprises the amino acid sequence IxFGDxYYT, in which x designates any amino acid residue. The enzyme exhibits activity towards acetylated xylan and acetylated mannan and may be used for modifying or degrading plant containing materials.
摘要翻译：具有乙酰酯酶活性的酶，该酶包含氨基酸序列IxFGDxYYT，其中x表示任何氨基酸残基。该酶显示出对乙酰化木聚糖和乙酰化甘露聚糖的活性，并可用于修饰或降解含植物材料。

10. 发明申请

WO1995002044A1 AN ENZYME WITH PROTEASE ACTIVITY 审中-公开
标题翻译：具有蛋白酶活性的酶
公开(公告)号：WO1995002044A1
公开(公告)日：1995-01-19
申请号：PCT/DK1994000274
申请日：1994-07-05
申请人： NOVO NORDISK A/S , DALBOGE, Henrik , CHRISTGAU, Stephan , ANDERSEN, Lene, Nonboe , KOFOD, Lene, Venke , KAUPPINEN, Markus, Sakari , NIELSEN, Jack, Bech , DAMBMANN, Claus
发明人： NOVO NORDISK A/S
IPC分类号： C12N09/62
CPC分类号： C11D3/3947 , A21D8/042 , A23K20/189 , C11D3/0078 , C11D3/386 , C11D3/38618 , C11D3/38636 , C12N9/62 , C12N9/82
摘要： A DNA construct comprising a DNA sequence encoding an enzyme exhibiting protease activity, which DNA sequence comprises the DNA sequence shown in SEQ ID No. 1 or 2 or an analogue of any of these sequences being at least 80 % homologous to the DNA sequence shown in SEQ ID No. 1 or 2. The proteases encoded by the DNA sequences have an acid pH optimum.
摘要翻译：包含编码表现出蛋白酶活性的酶的DNA序列的DNA构建体，所述DNA序列包含SEQ ID No.1或2所示的DNA序列或这些序列中的任一个的类似物与所示的DNA序列至少80％同源 SEQ ID No.1或2.由DNA序列编码的蛋白酶具有酸性pH最佳值。

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