会员体验
专利管家(专利管理)
工作空间(专利管理)
风险监控(情报监控)
数据分析(专利分析)
侵权分析(诉讼无效)
联系我们
交流群
官方交流:
QQ群: 891211   
微信请扫码    >>>
现在联系顾问~
热词
    • 9. 发明申请
    • ISOLATION OF MICROBIAL OILS
    • 分离微生物油
    • WO0210423A2
    • 2002-02-07
    • PCT/EP0108903
    • 2001-08-01
    • DSM NVBIJL HENDRIK LOUISSCHAAP ALBERT
    • BIJL HENDRIK LOUISSCHAAP ALBERT
    • C12M1/00A23D9/00B01D17/038C07G17/00C11B1/00C11B1/02C11B1/06C12P7/64
    • C12P7/6463A23D9/00C11B1/00C11B1/025C11B1/06C12P7/6472
    • The extraction of a microbial or single cell oil, for example comprising one or more polyunsaturated fatty acids (PUFAs), directly from microbial cells is disclosed which avoids the need for solvents. After fermentation, the microbial cells are pasteurised, washed and the cell walls lysed or disrupted by a mechanical (e.g. homogenisation), physical (boiling or drying), chemical (solvents) or enzymatic (cell wall degrading enzymes) technique. The oil (containing the PUFA) is then separated from the resulting cell wall debris. This is achieved by centrifugation, which results is an oily phase (top layer) that contains the oil which that can be separated from an aqueous phase (containing the cell wall debris). The oil can then be extracted and if necessary the PUFA can be purified or isolated from the oil.
    • 公开了直接从微生物细胞中提取微生物或单细胞油,例如包含一种或多种多不饱和脂肪酸(PUFA),其避免了溶剂的需要。 发酵后,将微生物细胞巴氏消毒,洗涤,并通过机械(如均化),物理(沸腾或干燥),化学(溶剂)或酶(细胞壁降解酶)技术裂解或破坏细胞壁。 然后将油(含有PUFA)与所得的细胞壁碎片分离。 这是通过离心实现的,其结果是油相(顶层),其含有可以从水相中分离的油(含有细胞壁碎片)。 然后可以提取油,如果需要,PUFA可以从油中纯化或分离。