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1. 发明申请

WO1997023633A1 IMPROVED METHODS FOR TRANSFORMING PHAFFIA STRAINS, TRANSFORMED PHAFFIA STRAINS SO OBTAINED AND RECOMBINANT DNA IN SAID METHODS 审中-公开
标题翻译：改进PHAFFIA菌株的改良方法，所获得的转化的PHAFFIA菌株和重组DNA在同一方法中
公开(公告)号：WO1997023633A1
公开(公告)日：1997-07-03
申请号：PCT/EP1996005887
申请日：1996-12-23
申请人： GIST-BROCADES B.V. , OOIJEN, Albert, Johannes, Joseph , VERDOES, Jan, Cornelis , WERY, Jan
发明人： GIST-BROCADES B.V. , OOIJEN, Albert, Johannes, Joseph
IPC分类号： C12N15/81
CPC分类号： C12P23/00 , C07K14/39 , C12N9/00 , C12N9/0004 , C12N9/0008 , C12N9/1085 , C12N9/90 , C12N15/52 , C12N15/815 , C12Y102/01012
摘要： The present invention provides recombinant DNA comprising a transcription promoter and a downstream sequence to be expressed, in operable linkage therewith, wherein the transcription promoter comprises a region found upstream of the open reading frame of a highly expressed Phaffia gene, preferably a glycolytic pathway gene, more preferably the gene coding for Glyceraldehyde-3-Phosphate Dehydrogenase. Further preferred recombinant DNAs according to the invention contain promoters of ribosomal protein encoding genes, more preferably wherein the transcription promoter comprises a region found upstream of the open reading frame encoding a protein as represented by one of the amino acid sequences depicted in any one of SEQIDNOs: 24 to 50. According to a further aspect of the invention an isolated DNA sequence coding for an enzyme involved in the carotenoid biosynthetic pathway of Phaffia rhodozyma is provided, preferably wherein said enzyme has an activity selected from isopentenyl pyrophosphate isomerase activity, geranylgeranyl pyrophosphate synthase activity, phytoene synthase activity, phytoene desaturase activity and lycopene cyclase activity, still more preferably those coding for an enzyme having an amino acid sequence selected from the one represented by SEQIDNO: 13, SEQIDNO: 15, SEQIDNO: 17, SEQIDNO: 19, SEQIDNO: 21 or SEQIDNO: 23. Further embodiments concern vectors, transformed host organisms, methods for making proteins and/or carotenoids, such as astaxanthin, and methods for isolating highly expressed promoters from Phaffia.
摘要翻译：本发明提供了包含转录启动子和待表达的下游序列的重组DNA，其可操作地连接于其中，其中转录启动子包含在高度表达的菲菲亚基因，优选糖酵解途径基因的开放阅读框的上游发现的区域，更优选为编码甘油醛-3-磷酸脱氢酶的基因。更优选的根据本发明的重组DNA包含核糖体蛋白质编码基因的启动子，更优选地，其中转录启动子包含编码如SEQ ID NO中任一个所示的氨基酸序列之一的开放阅读框上游的区域根据本发明的另一方面，提供了编码参与红发夫酵母的类胡萝卜素生物合成途径的酶的分离的DNA序列，优选其中所述酶具有选自异戊烯基焦磷酸异构酶活性，香叶基香叶基焦磷酸合酶活性，八氢番茄红素合成酶活性，八氢番茄红素去饱和酶活性和番茄红素环化酶活性，还更优选编码具有SEQ ID NO：13，SEQ ID NO：15，SEQ ID NO：17，SEQ ID NO：19，SEQ ID NO所代表的氨基酸序列的酶的那些：21或SEQ ID NO：23.其他实施方案涉及载体，转化宿主生物，用于制备蛋白质和/或类胡萝卜素的方法，例如虾青素，以及从Phaffia分离高表达启动子的方法。

2. 发明申请

WO9606935A3 ARABINOXYLAN DEGRADING ENZYMES 审中-公开
标题翻译：阿拉伯糖降解酶
公开(公告)号：WO9606935A3
公开(公告)日：1996-04-11
申请号：PCT/EP9503395
申请日：1995-08-28
申请人： GIST BROCADES BV , WOUW MONIQUE JOSINA ANDREA V D , OOIJEN ALBERT JOHANNES JOSEPH , GIELKENS MARCUS MATHEUS CATHAR , GRAAFF LEENDERT HENDRIK DE , VISSER JACOB
发明人： VAN DER WOUW MONIQUE JOSINA AN , VAN OOIJEN ALBERT JOHANNES JOS , GIELKENS MARCUS MATHEUS CATHAR , DE GRAAFF LEENDERT HENDRIK , VISSER JACOB
IPC分类号： A21D20060101 , A21D8/04 , A21D8/06 , A23G1/00 , A23K20060101 , A23K1/165 , C07H21/04 , C12N20060101 , C12N1/00 , C12N1/14 , C12N1/15 , C12N5/10 , C12N9/00 , C12N9/24 , C12N9/42 , C12N11/00 , C12N15/11 , C12N15/55 , C12R1/55 , C12R1/66 , C12S3/02 , D21C3/00
CPC分类号： C12Y302/01055 , C12N9/2402 , C12R1/66 , Y10S435/913 , Y10S435/917
摘要： Methods and expression constructs are provided for the cloning and overexpression of an arabinoxylan degrading enzyme of fungal origin in a selected microbial host cell. The enzyme is shown to be active in the degradation of water-insoluble solids obtained from maize. The enzyme can be used in the preparation of animal feed compositions, human food or in industrial processes.

3. 发明申请

WO9941386A3 NOVEL ENDO-XYLOGALACTURONASE 审中-公开
标题翻译：新型内切酶活性
公开(公告)号：WO9941386A3
公开(公告)日：2000-01-20
申请号：PCT/EP9900860
申请日：1999-02-09
申请人： DSM NV , MEEUWSEN PETRUS JOHANNES ALBER , VLUGT BERGMANS CECILE JOHANNA , VINCKEN JEAN PAUL , BELDMAN GERRIT , VORAGEN ALPHONS GERARD JOSEPH , HERWEIJER MARGARETA ADRIANA , OOIJEN ALBERT JOHANNES JOSEPH
发明人： MEEUWSEN PETRUS JOHANNES ALBER , VAN DER VLUGT-BERGMANS CECILE , VINCKEN JEAN PAUL , BELDMAN GERRIT , VORAGEN ALPHONS GERARD JOSEPH , HERWEIJER MARGARETA ADRIANA , VAN OOIJEN ALBERT JOHANNES JOS
IPC分类号： A23L2/84 , A23L29/00 , A23L29/231 , C12N9/24 , C12N15/55 , C12P21/00 , C12Q1/34 , A23L1/0524 , A23L1/06
CPC分类号： C12Y302/01171 , A23L2/84 , A23L29/06 , A23L29/231 , C12N9/2402 , C12Q1/34 , C12Y302/01015 , C12Y302/01099 , G01N2333/924
摘要： Polypeptides possessing a novel activity, namely endo-xylogalacturonase activity, are disclosed. These polypeptides can degrade pectin found in plant extracts and plant materials, and in particular the "hairy" regions of pectin polymers. In particular, the polypeptides can cleave in a galacturonic acid polymer at internal glycocidic bonds. The novel enzyme XghA is disclosed, and its amino acid sequence and encoding DNA sequence given. This polypeptide was expressed in yeast cells and has been used to treat vegetable material, in particular soy and fruit juice in the preparation of edible foodstuffs.
摘要翻译：公开了具有新活性的多肽，即内切木糖聚半乳糖醛酸酶活性。这些多肽可以降解在植物提取物和植物材料中发现的果胶，特别是果胶聚合物的“毛发”区域。特别地，多肽可以在内部糖苷键处在半乳糖醛酸聚合物中切割。公开了新型XghA酶，其氨基酸序列和编码DNA序列。该多肽在酵母细胞中表达，并且已经用于处理植物材料，特别是在可食用食品的制备中的大豆和果汁。

4. 发明申请

WO1996006935A2 ARABINOXYLAN DEGRADING ENZYMES 审中-公开
标题翻译：阿拉伯糖降解酶
公开(公告)号：WO1996006935A2
公开(公告)日：1996-03-07
申请号：PCT/EP1995003395
申请日：1995-08-28
申请人： GIST-BROCADES B.V. , VAN DER WOUW, Monique, Josina, Andrea , VAN OOIJEN, Albert, Johannes, Joseph , GIELKENS, Marcus, Matheus, Catharina , DE GRAAFF, Leendert, Hendrik , VISSER, Jacob
发明人： GIST-BROCADES B.V.
IPC分类号： C12N15/55
CPC分类号： C12Y302/01055 , C12N9/2402 , C12R1/66 , Y10S435/913 , Y10S435/917
摘要： Methods and expression constructs are provided for the cloning and overexpression of an arabinoxylan degrading enzyme of fungal origin in a selected microbial host cell. The enzyme is shown to be active in the degradation of water-insoluble solids obtained from maize. The enzyme can be used in the preparation of animal feed compositions, human food or in industrial processes.
摘要翻译：提供了方法和表达构建体用于在选定的微生物宿主细胞中克隆和过表达真菌来源的阿拉伯木聚糖降解酶。显示该酶在从玉米获得的水不溶性固体的降解中是有活性的。该酶可用于制备动物饲料组合物，人类食物或工业过程。

5. 发明申请

WO1995035362A1 CLEANING COMPOSITIONS CONTAINING PLANT CELL WALL DEGRADING ENZYMES AND THEIR USE IN CLEANING METHODS 审中-公开
标题翻译：含有植物细胞壁降解酶的清洁组合物及其在清洁方法中的应用
公开(公告)号：WO1995035362A1
公开(公告)日：1995-12-28
申请号：PCT/EP1995002380
申请日：1995-06-19
申请人： GIST-BROCADES B.V. , CUPERUS, Roulck, Anneke , HERWEIJER, Margareta, Adriana , VAN OOIJEN, Albert, Johannes, Joseph , VAN SCHOUWEN, Dick, Johannes
发明人： GIST-BROCADES B.V.
IPC分类号： C11D03/386
CPC分类号： C11D3/38636 , C11D3/38645
摘要： Novel cleaning compositions comprising cell wall degrading enzymes are disclosed having pectinases and/or hemicellulases and optionally cellulases. The compositions are particularly suitable for removing stains of vegetable origin, especially from textiles. Although compositions having only one type of such enzymes are part of the invention (excluding cellulases alone), preferred embodiments have a mixture of cell wall degrading enzyme activities to allow for a concerted action against the fibrous mass which usually constitutes a stain of vegetable origin.
摘要翻译：公开了包含细胞壁降解酶的新型清洁组合物，其具有果胶酶和/或半纤维素酶和任选的纤维素酶。该组合物特别适用于去除植物来源的污渍，特别是纺织品的污渍。虽然仅具有一种类型的这种酶的组合物是本发明的一部分（不包括纤维素酶），但是优选的实施方案具有细胞壁降解酶活性的混合物，以允许针对通常构成植物来源的污渍的纤维块的协同作用。

6. 发明申请

WO1995029598A1 ENZYMATIC TREATMENT OF SOY 审中-公开
标题翻译：大豆的酶处理
公开(公告)号：WO1995029598A1
公开(公告)日：1995-11-09
申请号：PCT/EP1995001685
申请日：1995-05-02
申请人： GIST-BROCADES B.V. , VAN OOIJEN, Albert, Johannes, Joseph , SCHUURHUIZEN, Paul, William , EDENS, Luppo , BEVERINI, Marc
发明人： GIST-BROCADES B.V.
IPC分类号： A23L01/211
CPC分类号： C12Y302/01004 , A23L11/33 , C12Y302/01008 , C12Y302/01015 , C12Y302/01021 , C12Y302/01055 , C12Y302/01099 , C12Y304/00 , C12Y402/0201
摘要： The present invention describes an enzymatical method for the treatment of soy suspensions, to obtain a suspension with a low waterbinding capacity and a low viscosity. The treatment is accomplished using a limited amount of critical enzymes. A soy composition containing a reduced amount of monosaccharides and uronic acids is obtained.
摘要翻译：本发明描述了用于处理大豆悬浮液的酶法，以获得具有低结合能力和低粘度的悬浮液。使用有限量的关键酶完成治疗。得到含有减少量的单糖和糖醛酸的大豆组合物。

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