专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

1. 发明申请

WO2009135214A3 RNA POLYPHOSPHATASE COMPOSITIONS, KITS, AND USES THEREOF 审中-公开
标题翻译： RNA多磷酸酯组合物，胶囊及其用途
公开(公告)号：WO2009135214A3
公开(公告)日：2010-03-11
申请号：PCT/US2009042729
申请日：2009-05-04
申请人： JENDRISAK JEROME J , VAIDYANATHAN RAMESH , MEIS RONALD , EPICT TECHNOLOGIES CORP
发明人： JENDRISAK JEROME J , VAIDYANATHAN RAMESH , MEIS RONALD
IPC分类号： C12N9/14 , C12Q1/42 , C12Q1/68
CPC分类号： C12Q1/42 , C12N9/14 , C12P19/34 , Y10T436/143333
摘要： The present invention relates to the discovery of RNA 5' polyphosphatase enzymes not previously described in the art, methods for discovery of said enzymes, compositions of said enzymes, methods for making said enzymes, and various methods and kits for using said enzymes for biomedical research, for human and non-human diagnostics, for production of therapeutic products, and for other applications. In particular, some embodiments provide compositions, kits and methods for employing RNA polyphosphatases for isolation, purification, production, and assay of capped RNA using a biological sample or a sample from an in vitro capping reaction wherein the sample also contains RNA that is not capped. Other embodiments provide compositions, kits and methods wherein RNA polyphosphatases comprise signal-amplifying enzymes for analyte-specific assays.
摘要翻译：本发明涉及本领域先前未描述的RNA 5'多磷酸酶的发现，所述酶的发现方法，所述酶的组合物，所述酶的制备方法以及使用所述酶进行生物医学研究的各种方法和试剂盒，用于人和非人诊断，用于生产治疗产品和其他应用。具体地，一些实施方案提供了使用RNA多磷酸酶用于使用生物样品或来自体外封端反应的样品分离，纯化，生产和测定加帽RNA的组合物，试剂盒和方法，其中所述样品还含有未被封端的RNA 。其他实施方案提供组合物，试剂盒和方法，其中RNA多磷酸酶包含用于分析物特异性测定的信号放大酶。

2. 发明申请

WO2006086668A2 COMPOSITIONS AND METHODS EMPLOYING 5'-PHOSPHATE-DEPENDENT NUCLEIC ACID EXONUCLEASES 审中-公开
标题翻译：使用5'-磷酸依赖性核酸脱氢酶的组合物和方法
公开(公告)号：WO2006086668A2
公开(公告)日：2006-08-17
申请号：PCT/US2006004798
申请日：2006-02-09
申请人： EPICT TECHNOLOGIES , JENDRISAK JEROME , MEIS JUDITH , MEIS RONALD , RADEK AGNES , DAHL GARY
发明人： JENDRISAK JEROME , MEIS JUDITH , MEIS RONALD , RADEK AGNES , DAHL GARY
IPC分类号： C12Q1/68
CPC分类号： C12N15/1006 , C12N15/1096 , C12Q1/6806 , C12Q2521/319
摘要： The present invention relates to compositions and methods employing 5'- phosphate-dependent nucleic acid exonucleases. In particular, the present invention provides kits and methods employing 5 '-phosphate-dependent nucleic acid exonucleases for selective enrichment, isolation and amplification of a particular set of desired nucleic acid molecules from samples that also contain undesired nucleic acid molecules for a variety of uses. In preferred embodiments, the desired nucleic acid molecules comprise prokaryotic and/or eukaryotic mRNA.
摘要翻译：本发明涉及使用5'-磷酸依赖性核酸外切核酸酶的组合物和方法。特别地，本发明提供了使用5'-磷酸依赖性核酸外切核酸酶用于选择性富集，分离和扩增特定的一组所需核酸分子的试剂盒和方法，该样品还含有用于各种用途的不需要的核酸分子的样品。在优选的实施方案中，所需的核酸分子包含原核和/或真核的mRNA。

3. 发明申请

WO2007120863A3 KITS AND METHODS FOR GENERATING 5' CAPPED RNA 审中-公开
标题翻译：用于产生5'CAPPED RNA的试剂盒和方法
公开(公告)号：WO2007120863A3
公开(公告)日：2008-10-30
申请号：PCT/US2007009217
申请日：2007-04-16
申请人： EPICT TECHNOLOGIES , JENDRISAK JEROME , MEIS RONALD , DAHL GARY
发明人： JENDRISAK JEROME , MEIS RONALD , DAHL GARY
IPC分类号： C12N15/11 , C12N15/113 , C12N15/85 , C12P19/34
CPC分类号： C12P19/34 , C12N9/1007 , C12N9/1241 , C12N15/1072 , C12N15/1075 , C12N15/1096 , C12N15/111 , C12N15/113 , C12N2310/317 , C12N2320/51 , C12Y201/01056 , C12Y201/01057 , C12Y207/07019 , C12Y207/0705
摘要： The present invention relates to kits and methods for efficiently generating 5' capped RNA having a modified cap nucleotide and for use of such modified-nucleotide-capped RNA molecules. The invention is used to obtain novel compositions of such modified- nucleotide-capped RNA molecules. In particular, the present invention provides kits and methods for capping RNA using a modified cap nucleotide and a capping enzyme system, such as poxvirus capping enzyme. The present invention finds use for in vitro production o 5'-capped RNA having a modified cap nucleotide and for in vitro or in vivo production o polypeptides by in vitro or in vivo translation of such modified-nucleotide-capped RNA for a variety of research, therapeutic, and commercial applications. The invention also provides methods and kits for capturing or isolating uncapped RNA comprising primary RNA transcripts or RNA having a 5'-diphosphate, such as RNA synthesized in vitro or obtained from a biological source, including prokaryotic mRNA that is in a mixture with other prokaryotic and/or eukaryotic nucleic acids. The method for capturing modified-nucleotide- capped RNA also provides methods and kits for obtaining only type-specific or condition- specific modified-nucleotide-capped RNA by cap-dependent subtraction of that portion of the captured modified-nucleotide-capped RNA in cells of one type or condition that is the same as RNA in cells of another type or condition. The invention further provides methods and kits for using a capping enzyme system and modified cap nucleotides for labelin uncapped RNA comprising primary RNA transcripts or RNA having a 5 '-diphosphate with detectable dye or enzyme moieties.
摘要翻译：本发明涉及用于有效产生具有修饰的帽核苷酸的5'封端RNA并用于这种修饰的核苷酸封端RNA分子的试剂盒和方法。本发明用于获得这种经修饰的核苷酸封端RNA分子的新组合物。特别地，本发明提供了使用修饰的帽核苷酸和封端酶系统（例如痘病毒覆盖酶）对RNA进行封端的试剂盒和方法。本发明用于体外生产o具有修饰的帽核苷酸的体外或体内生产的5'-封端RNA，通过体外或体内翻译这些经修饰的核苷酸封端的RNA进行多种研究，治疗和商业应用。本发明还提供用于捕获或分离未封端RNA的方法和试剂盒，其包含初级RNA转录物或具有5'-二磷酸的RNA，例如体外合成或从生物来源获得的RNA，包括与其他原核生物混合的原核mRNA 和/或真核的核酸。用于捕获修饰的核苷酸封端RNA的方法还提供了通过在细胞中捕获的修饰的核苷酸加帽的RNA的那部分的帽依赖性减法来获得仅特定类型或条件特异性修饰的核苷酸加帽的RNA的方法和试剂盒与另一种类型或病症细胞中的RNA相同的一种类型或病症。本发明还提供了用于使用封端酶系统和修饰的帽子核苷酸用于标记未封端RNA的方法和试剂盒，其包含具有可检测染料或酶部分的5'-二磷酸的初级RNA转录物或RNA。

4. 发明申请

WO2007120863A2 KITS AND METHODS FOR GENERATING 5' CAPPED RNA 审中-公开
标题翻译：用于产生5'CAPPED RNA的试剂盒和方法
公开(公告)号：WO2007120863A2
公开(公告)日：2007-10-25
申请号：PCT/US2007/009217
申请日：2007-04-16
申请人： EPICENTRE TECHNOLOGIES , JENDRISAK, Jerome , MEIS, Ronald , DAHL, Gary
发明人： JENDRISAK, Jerome , MEIS, Ronald , DAHL, Gary
IPC分类号： C07H21/00 , C07H21/02 , C12N5/04 , C12N5/10 , C12N9/00 , C12N9/10 , C12P19/34
CPC分类号： C12P19/34 , C12N9/1007 , C12N9/1241 , C12N15/1072 , C12N15/1075 , C12N15/1096 , C12N15/111 , C12N15/113 , C12N2310/317 , C12N2320/51 , C12Y201/01056 , C12Y201/01057 , C12Y207/07019 , C12Y207/0705
摘要： The present invention relates to kits and methods for efficiently generating 5' capped RNA having a modified cap nucleotide and for use of such modified-nucleotide-capped RNA molecules. The invention is used to obtain novel compositions of such modified- nucleotide-capped RNA molecules. In particular, the present invention provides kits and methods for capping RNA using a modified cap nucleotide and a capping enzyme system, such as poxvirus capping enzyme. The present invention finds use for in vitro production o 5'-capped RNA having a modified cap nucleotide and for in vitro or in vivo production o polypeptides by in vitro or in vivo translation of such modified-nucleotide-capped RNA for a variety of research, therapeutic, and commercial applications. The invention also provides methods and kits for capturing or isolating uncapped RNA comprising primary RNA transcripts or RNA having a 5'-diphosphate, such as RNA synthesized in vitro or obtained from a biological source, including prokaryotic mRNA that is in a mixture with other prokaryotic and/or eukaryotic nucleic acids. The method for capturing modified-nucleotide- capped RNA also provides methods and kits for obtaining only type-specific or condition- specific modified-nucleotide-capped RNA by cap-dependent subtraction of that portion of the captured modified-nucleotide-capped RNA in cells of one type or condition that is the same as RNA in cells of another type or condition. The invention further provides methods and kits for using a capping enzyme system and modified cap nucleotides for labelin uncapped RNA comprising primary RNA transcripts or RNA having a 5 '-diphosphate with detectable dye or enzyme moieties.
摘要翻译：本发明涉及用于有效产生具有修饰的帽核苷酸的5'封端RNA并用于这种修饰的核苷酸封端RNA分子的试剂盒和方法。本发明用于获得这种经修饰的核苷酸封端RNA分子的新组合物。特别地，本发明提供了使用修饰的帽核苷酸和封端酶系统（例如痘病毒覆盖酶）对RNA进行封端的试剂盒和方法。本发明用于体外生产o具有修饰的帽核苷酸的体外或体内生产的5'-封端RNA，通过体外或体内翻译这些经修饰的核苷酸封端的RNA进行多种研究，治疗和商业应用。本发明还提供用于捕获或分离未封端RNA的方法和试剂盒，其包含初级RNA转录物或具有5'-二磷酸的RNA，例如体外合成或从生物来源获得的RNA，包括与其他原核生物混合的原核mRNA 和/或真核的核酸。用于捕获修饰的核苷酸封端RNA的方法还提供了通过在细胞中捕获的修饰的核苷酸加帽的RNA的那部分的帽依赖性减法来获得仅特定类型或条件特异性修饰的核苷酸加帽的RNA的方法和试剂盒与另一种类型或病症细胞中的RNA相同的一种类型或病症。本发明还提供了用于使用封端酶系统和修饰的帽子核苷酸用于标记未封端RNA的方法和试剂盒，其包含具有可检测染料或酶部分的5'-二磷酸的初级RNA转录物或RNA。

5. 发明申请

WO2006086668A3 COMPOSITIONS AND METHODS EMPLOYING 5'-PHOSPHATE-DEPENDENT NUCLEIC ACID EXONUCLEASES 审中-公开
公开(公告)号：WO2006086668A3
公开(公告)日：2006-08-17
申请号：PCT/US2006/004798
申请日：2006-02-09
申请人： EPICENTRE TECHNOLOGIES , JENDRISAK, Jerome , MEIS, Judith , MEIS, Ronald , RADEK, Agnes , DAHL, Gary
发明人： JENDRISAK, Jerome , MEIS, Judith , MEIS, Ronald , RADEK, Agnes , DAHL, Gary
IPC分类号： C12P19/34
摘要： The present invention relates to compositions and methods employing 5'- phosphate-dependent nucleic acid exonucleases. In particular, the present invention provides kits and methods employing 5 '-phosphate-dependent nucleic acid exonucleases for selective enrichment, isolation and amplification of a particular set of desired nucleic acid molecules from samples that also contain undesired nucleic acid molecules for a variety of uses. In preferred embodiments, the desired nucleic acid molecules comprise prokaryotic and/or eukaryotic mRNA.

6. 发明申请

WO2009135214A2 RNA POLYPHOSPHATASE COMPOSITIONS, KITS, AND USES THEREOF 审中-公开
标题翻译： RNA多磷酸酯组合物，胶囊及其用途
公开(公告)号：WO2009135214A2
公开(公告)日：2009-11-05
申请号：PCT/US2009/042729
申请日：2009-05-04
申请人： EPICENTRE TECHNOLOGIES CORPORATION , JENDRISAK, Jerome, J. , VAIDYANATHAN, Ramesh , MEIS, Ronald
发明人： JENDRISAK, Jerome, J. , VAIDYANATHAN, Ramesh , MEIS, Ronald
IPC分类号： C12N9/14 , C12Q1/42 , C12Q1/68
CPC分类号： C12Q1/42 , C12N9/14 , C12P19/34 , Y10T436/143333
摘要： The present invention relates to the discovery of RNA 5' polyphosphatase enzymes not previously described in the art, methods for discovery of said enzymes, compositions of said enzymes, methods for making said enzymes, and various methods and kits for using said enzymes for biomedical research, for human and non-human diagnostics, for production of therapeutic products, and for other applications. In particular, some embodiments provide compositions, kits and methods for employing RNA polyphosphatases for isolation, purification, production, and assay of capped RNA using a biological sample or a sample from an in vitro capping reaction wherein the sample also contains RNA that is not capped. Other embodiments provide compositions, kits and methods wherein RNA polyphosphatases comprise signal-amplifying enzymes for analyte-specific assays.
摘要翻译：本发明涉及本领域先前未描述的RNA 5'多磷酸酶的发现，所述酶的发现方法，所述酶的组合物，所述酶的制备方法以及使用所述酶进行生物医学研究的各种方法和试剂盒，用于人和非人诊断，用于生产治疗产品和其他应用。具体地，一些实施方案提供了使用RNA多磷酸酶用于使用生物样品或来自体外封端反应的样品分离，纯化，生产和测定加帽RNA的组合物，试剂盒和方法，其中所述样品还含有未被封端的RNA 。其他实施方案提供组合物，试剂盒和方法，其中RNA多磷酸酶包含用于分析物特异性测定的信号放大酶。

你已经成功收藏专利！

检索式保存成功!

IPRDB

热门服务

关于我们

友情链接

联系方式