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1. 发明申请

WO1998027224A1 FLUORESCENT ENZYME SUBSTRATES AND METHOD FOR ASSAYING ENZYMATIC ACTIVITY 审中-公开
标题翻译：荧光酶基质和测定酶活性的方法
公开(公告)号：WO1998027224A1
公开(公告)日：1998-06-25
申请号：PCT/JP1997004631
申请日：1997-12-16
申请人： LABORATORY OF MOLECULAR BIOPHOTONICS , SHIONO, Hirofumi , NOHTA, Hitoshi , UTSUYAMA, Chika
发明人： LABORATORY OF MOLECULAR BIOPHOTONICS
IPC分类号： C12Q01/42
CPC分类号： C12Q1/34 , C12Q2334/00 , C12Q2334/20
摘要： Enzyme substrates each carrying in its molecule a group which is cleaved by the enzymatic reaction and another group which is cleaved by the enzymatic reaction and then forms a coumarin derivative by intramolecular lactonization. A method for assaying enzymatic activity which comprises effecting an enzymatic reaction with the use of the above substrate and then measuring the fluorescence of the coumarin derivative thus formed to thereby determine the enzymatic activity.
摘要翻译：各自携带分子中的酶底物是通过酶促反应切割的基团和另一个被酶反应切割的基团，然后通过分子内内酯形成香豆素衍生物。用于测定酶活性的方法，其包括使用上述底物进行酶促反应，然后测量由此形成的香豆素衍生物的荧光从而确定酶活性。

2. 发明申请

WO1998011210A1 SOLID PHASE FOR TARGET NUCLEIC ACID DETECTION, PROCESS FOR PRODUCTION THEREOF, AND METHOD OF TARGET NUCLEIC ACID DETECTION 审中-公开
标题翻译：用于目标核酸检测的固相，其生产方法和靶核酸检测方法
公开(公告)号：WO1998011210A1
公开(公告)日：1998-03-19
申请号：PCT/JP1997003232
申请日：1997-09-12
申请人： LABORATORY OF MOLECULAR BIOPHOTONICS , ABE, Satoshi , SATO, Yoshihiro
发明人： LABORATORY OF MOLECULAR BIOPHOTONICS
IPC分类号： C12N15/11
CPC分类号： C12Q1/6834 , C12Q2537/143 , C12Q2533/107 , C12Q2525/307
摘要： A solid phase for target nucleic acid detection which has a base sequence hybridizable in sequence with a specified polynucleotide sequence of a target nucleic acid and contains a set of probes with their spatial arrangement immobilized onto the solid phase through a linker portion so as to be enzymatically ligated during the hybridization; and a method of target nucleic acid detection which comprises hybridizing the solid phase with the target nucleic acid, ligating the probes by a ligase reaction, and detecting the product of ligation.
摘要翻译：用于靶核酸检测的固相，其具有与靶核酸的指定多核苷酸序列可序列杂交的碱基序列，并且含有一组其空间排列的探针通过接头部分固定在固相上以便酶促在杂交期间连接; 和靶核酸检测方法，其包括将固相与靶核酸杂交，通过连接酶反应连接探针，并检测连接产物。

3. 发明申请

WO1997047968A1 HIGHLY SENSITIVE FLUORESCENT IMMUNOASSAY 审中-公开
标题翻译：高敏感荧光免疫
公开(公告)号：WO1997047968A1
公开(公告)日：1997-12-18
申请号：PCT/JP1997001960
申请日：1997-06-09
申请人： LABORATORY OF MOLECULAR BIOPHOTONICS , HOSOI, Shigeru , KOJIMA, Makiko , KADOUCHI, Sachiko
发明人： LABORATORY OF MOLECULAR BIOPHOTONICS
IPC分类号： G01N33/533
CPC分类号： G01N33/582 , C12Q1/6834 , G01N33/533 , Y10S435/968 , Y10S436/828 , C12Q2563/107 , C12Q2545/114 , C12Q2565/601
摘要： A fluorescent immunoassay characterized by labeling a sample with a fluorescent marker having a nucleic acid moiety stained with such a sufficient number of fluorochromes as to enable the measurement of fluorescent spots and a reactive group capable of binding specifically to the sample, immobilizing the sample thus labeled onto a solid phase, and then counting the fluorescent spots. The nucleic acid moiety of the fluorescent marker is a double- or single-stranded nucleic acid. Staining with the fluorochromes is effected with the use of intercalater type fluorochrome(s), mirror group type fluorochrome(s), and fluorochrome(s) covalently bonded to the nucleic acid.
摘要翻译：荧光免疫测定法，其特征在于用荧光标记物标记样品，所述荧光标记具有用足够数量的荧光染料染色的核酸部分，以便能够测量荧光斑点和能够特异性结合样品的反应性基团，固定如此标记的样品固相，然后计数荧光斑点。荧光标记物的核酸部分是双链或单链核酸。用荧光染料染色是通过使用与核酸共价结合的嵌入型荧光染料，镜式荧光染料和荧光染料来实现的。

4. 发明申请

WO1997047639A1 PHOTOCLEAVABLE CYCLIC OLIGONUCLEOTIDE 审中-公开
标题翻译：可溶性环状寡核苷酸
公开(公告)号：WO1997047639A1
公开(公告)日：1997-12-18
申请号：PCT/JP1997001959
申请日：1997-06-09
申请人： LABORATORY OF MOLECULAR BIOPHOTONICS , SHIONO, Hirofumi , KODAMA, Hirofumi , KOJIMA, Makiko
发明人： LABORATORY OF MOLECULAR BIOPHOTONICS
IPC分类号： C07H21/02
CPC分类号： C07H21/00
摘要： A photocleavable cyclic oligonucleotide having a base sequence capable of hybridizing with a target DNA or RNA and a structure cyclized with a photocleavable linkage. The oligonucleotide is not decomposed by nucleases in the living body owing to its cyclic structure, so that it can be diffused to predetermined positions in the body by taking a sufficiently prolonged time. Further, the linkage is cleaved by irradiation with a suitable light after the lapse of a predetermined time, by which the cyclic oligonucleotide is converted into a linear one, thus acting as an antisense oligonucleotide.
摘要翻译：具有能够与靶DNA或RNA杂交的碱基序列的可光切割的环状寡核苷酸和可光切割性连接环化的结构。寡核苷酸由于其循环结构而不被生物体中的核酸酶分解，从而可以通过充分延长时间将其扩散到体内的预定位置。此外，在经过预定时间之后，通过用合适的光照射使得连接被切割，由此将环状寡核苷酸转化为线性寡核苷酸，从而起到反义寡核苷酸的作用。

5. 发明申请

WO1997032198A1 METHOD AND APPARATUS FOR ASSAYING ENZYMATIC REACTION 审中-公开
标题翻译：用于测定酶促反应的方法和装置
公开(公告)号：WO1997032198A1
公开(公告)日：1997-09-04
申请号：PCT/JP1997000513
申请日：1997-02-24
申请人： LABORATORY OF MOLECULAR BIOPHOTONICS , OKAZAKI, Shigetoshi , MATSUMOTO, Hiroyuki
发明人： LABORATORY OF MOLECULAR BIOPHOTONICS
IPC分类号： G01N21/75
CPC分类号： G01N21/552
摘要： A method and apparatus for determining enzyme activities by supplying a substrate solution and an enzyme solution to a reaction vessel to which a total reflection absorption prism is connected. An assay solution (40) comprising a mixture of an enzyme solution and a substrate solution is brought to a constant temperature in a reaction vessel (26) by a thermostat (23) and stirred by an agitator (21). An infrared ray (36) sent out of an infrared ray source (3) enters an interface between a total reflection absorption prism (28) in contact with the assay solution (40) and the solution (40) from the side of the prism (28), and is totally reflected thereupon. The spectrum of the totally reflected, outgoing transmitted infrared ray (37) is detected by an infrared ray detector (5), and the variation of the infrared ray absorption spectrum or absorbance is determined on the basis of what has been detected, whereby the enzyme reaction in the object solution (40) is assayed.
摘要翻译：一种通过向连接有全反射吸收棱镜的反应容器提供底物溶液和酶溶液来测定酶活性的方法和装置。将包含酶溶液和底物溶液的混合物的测定溶液（40）通过恒温器（23）在反应容器（26）中达到恒定温度，并通过搅拌器（21）搅拌。从红外线源（3）发出的红外线（36）进入与测定溶液（40）接触的全反射吸收棱镜（28）和来自棱镜侧的溶液（40）之间的界面 28），并完全反映在那里。通过红外线检测器（5）检测全反射出射透射红外线（37）的光谱，基于检测到的内容来确定红外线吸收光谱或吸光度的变化，由此酶测定目标溶液（40）中的反应。

6. 发明申请

WO1998013676A1 METHOD AND INSTRUMENT FOR POLARIZATION MEASUREMENT 审中-公开
标题翻译：用于偏振测量的方法和仪器
公开(公告)号：WO1998013676A1
公开(公告)日：1998-04-02
申请号：PCT/JP1997003391
申请日：1997-09-24
申请人： LABORATORY OF MOLECULAR BIOPHOTONICS , TOYONAGA, Shuji , SHIROSHITA, Masahisa , SUGA, Takayuki , NAKANO, Yoshitaro
发明人： LABORATORY OF MOLECULAR BIOPHOTONICS
IPC分类号： G01J04/00
CPC分类号： G01N21/6445 , G01J3/4406 , G01J3/447 , G01J4/00 , G01N21/65
摘要： The polarization of fluorescent light or Raman scattering light which is emitted from a sample when light is applied to the sample is measured with high accuracy. Excitation light which is emitted from a pulse excitation light source (1) and is p-polarized by a polarizer (2) and a half-wave plate (3) is applied to a sample (7) and the p-polarization component intensity Ipp and s-polarization component intensity Ips of the emitted fluorescent light are measured by photodetectors (13 and 14). In the same way, s-polarized excitation light is applied to the sample (7) and the p-polarization component intensity Isp and s-polarization component intensity Iss of emitted fluorescent light are measured. The G-factor is obtained from those measured values by a following formula: G = [(Ipp.Isp)/(Ips.Iss)] . Polarization responsiveness correction is performed in accordance with the G-factor to obtain the polarization of the fluorescent light.
摘要翻译：以高精度测量向样品施加光时从样品发出的荧光或拉曼散射光的极化。从脉冲激励光源（1）发射并由偏振器（2）和半波片（3）p极化的激发光施加到样品（7）上，并且p偏振分量强度Ipp 并且通过光电检测器（13和14）测量发射的荧光的s偏振分量强度Ips。以相同的方式，对样品（7）施加s偏振激发光，并测量发射荧光的p偏振分量强度Isp和s偏振分量强度Iss。 G因子通过下式由这些测量值获得：G = [（Ipp.Isp）/（Ips.Iss）] 1/2。根据G因子执行极化响应性校正，以获得荧光的偏振。

7. 发明申请

WO1998013524A1 PROBES FOR DETECTING POLYNUCLEOTIDES AND DETECTION METHOD 审中-公开
标题翻译：用于检测聚合物和检测方法的探针
公开(公告)号：WO1998013524A1
公开(公告)日：1998-04-02
申请号：PCT/JP1997003438
申请日：1997-09-26
申请人： LABORATORY OF MOLECULAR BIOPHOTONICS , SATO, Yoshihiro , TSUJI, Akihiko , SUGA, Takayuki
发明人： LABORATORY OF MOLECULAR BIOPHOTONICS
IPC分类号： C12Q01/68
CPC分类号： C12Q1/6818
摘要： Detection probes enabling very convenient and highly accurate and sensitive detection of DNAs or RNAs having specific base sequences in specimens in the presence of the probes in excess of the target nucleic acid; and a detection method. When mixed with a specimen containing the target substance (DNA, RNA, etc.) having a polynucleotide having a specific base sequence, two types of fluorescent label detection probes hybridize, while being adjacent to each other, with the target nucleic acid. As a result, there arises a shift of the resonance energy between the two types of fluorescent dye molecules. When the resonance energy shift occurs in the hybrid thus formed, the fluorescence attenuation of the acceptor fluorescent dye molecule can be sufficiently delayed compared with the fluorescence attenuation of the directly excited acceptor by appropriately regulating (1) the number of bases between the two nucleotides to which the fluorescent dye moleculees have bonded; (2) the structure (double- or single-stranded) of the hybrid between the two nucleotides to which the fluorescent dye molecules have bonded; and (3), on the detection probes, the sites of the nucleotides into which the fluorescent dye molecules are to be introduced; and by (4) using a pair of detection probes with the appropriate selection of the fluorescent dye molecules. The detection method comprises using the above-mentioned detection probes and detecting the target substance by measuring changes in the fluorescence attenuation curve of the acceptor after the irradiation with pulse excitation rays, thus enabling highly accurate and sensitive detection of the target nucleic acid in the presence of the detection probes in excess of the target nucleic acid.
摘要翻译：检测探针能够在超过目标核酸的探针存在下，在标本中具有特异性碱基序列的DNA或RNAs的非常方便和高度准确和灵敏的检测; 和检测方法。当与含有具有特定碱基序列的多核苷酸的靶物质（DNA，RNA等）的样品混合时，两种类型的荧光标记检测探针在彼此相邻的情况下与靶核酸杂交。结果，在两种类型的荧光染料分子之间产生共振能量的偏移。当共轭能量偏移发生在如此形成的杂化物中时，与直接激发的受体的荧光衰减相比，受体荧光染料分子的荧光衰减可以通过适当地调节（1）两个核苷酸之间的碱基数荧光染料分子结合; （2）荧光染料分子键合的两个核苷酸之间的杂交体的结构（双链或单链）和（3），在检测探针上，将引入荧光染料分子的核苷酸位点; 并且通过（4）使用具有适当选择荧光染料分子的一对检测探针。检测方法包括使用上述检测探针并通过测量在用脉冲激发射线照射之后受体的荧光衰减曲线的变化来检测目标物质，从而使得能够在存在下高度精确和灵敏地检测靶核酸的检测探针超过目标核酸。

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