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1. 发明申请

WO1997032198A1 METHOD AND APPARATUS FOR ASSAYING ENZYMATIC REACTION 审中-公开
标题翻译：用于测定酶促反应的方法和装置
公开(公告)号：WO1997032198A1
公开(公告)日：1997-09-04
申请号：PCT/JP1997000513
申请日：1997-02-24
申请人： LABORATORY OF MOLECULAR BIOPHOTONICS , OKAZAKI, Shigetoshi , MATSUMOTO, Hiroyuki
发明人： LABORATORY OF MOLECULAR BIOPHOTONICS
IPC分类号： G01N21/75
CPC分类号： G01N21/552
摘要： A method and apparatus for determining enzyme activities by supplying a substrate solution and an enzyme solution to a reaction vessel to which a total reflection absorption prism is connected. An assay solution (40) comprising a mixture of an enzyme solution and a substrate solution is brought to a constant temperature in a reaction vessel (26) by a thermostat (23) and stirred by an agitator (21). An infrared ray (36) sent out of an infrared ray source (3) enters an interface between a total reflection absorption prism (28) in contact with the assay solution (40) and the solution (40) from the side of the prism (28), and is totally reflected thereupon. The spectrum of the totally reflected, outgoing transmitted infrared ray (37) is detected by an infrared ray detector (5), and the variation of the infrared ray absorption spectrum or absorbance is determined on the basis of what has been detected, whereby the enzyme reaction in the object solution (40) is assayed.
摘要翻译：一种通过向连接有全反射吸收棱镜的反应容器提供底物溶液和酶溶液来测定酶活性的方法和装置。将包含酶溶液和底物溶液的混合物的测定溶液（40）通过恒温器（23）在反应容器（26）中达到恒定温度，并通过搅拌器（21）搅拌。从红外线源（3）发出的红外线（36）进入与测定溶液（40）接触的全反射吸收棱镜（28）和来自棱镜侧的溶液（40）之间的界面 28），并完全反映在那里。通过红外线检测器（5）检测全反射出射透射红外线（37）的光谱，基于检测到的内容来确定红外线吸收光谱或吸光度的变化，由此酶测定目标溶液（40）中的反应。

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