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1. 发明申请

WO2016181070A1 ENRICHISSEMENT ET CULTURE SELECTIVE DE SALMONELLA ET DE SHIGELLA 审中-公开
标题翻译：沙门氏菌和鲨鱼的增殖和选择性文化
公开(公告)号：WO2016181070A1
公开(公告)日：2016-11-17
申请号：PCT/FR2016/051102
申请日：2016-05-11
申请人： BIOMÉRIEUX
发明人： BASSO, Emanuelle , ROCHE, Jean-Marc
IPC分类号： C12N1/20
CPC分类号： C12Q1/045 , C12N1/20 , C12P13/10 , C12Q1/10 , C12Q2334/00 , C12R1/42 , G01N2333/255 , G01N2333/918 , Y02A50/451
摘要： L'invention a trait à la détection et l'identification de bactéries appartenant aux genres Salmonella et Shigella . Elle concerne plus précisément les méthodes de microbiologie et les milieux de culture utilisés pour la détection, l'identification, l'isolement et/ou l'étude analytique de ces bactéries. L'invention proposée porte sur un procédé d'enrichissement et de culture sélective de bactéries appartenant aux genres Salmonella et/ou Shigella contenues dans un échantillon biologique. Dans ledit procédé,tout ou partie dudit échantillon est ensemencé dans/sur un milieu de culture comprenant une composante nutritive propice au développement et à la croissance desdites bactéries,et comprenant de la L-ornithine, à titre d'agent sélectif. L'invention s'étend également à un milieu de culture adapté à la mise en œuvre de ce procédé.
摘要翻译：本发明涉及沙门菌属和志贺氏菌属细菌的检测和鉴定。更具体地，本发明涉及用于所述细菌的检测，鉴定，分离和/或分析研究的微生物学方法和培养基。本发明还涉及生物样品中所含的沙门氏菌属和/或志贺氏菌属的细菌的富集和选择性培养的方法。在所述方法中，将所有或部分所述样品接种在包含有利于所述细菌发育和生长的营养成分的培养基中，并且包含L-鸟氨酸作为选择剂。本发明还涉及一种用于实施所述方法的培养基。

2. 发明申请

WO2006107583A1 TEST MEDIA AND QUANTITATIVE OR QUALITATIVE METHOD FOR IDENTIFICATION AND DIFFERENTIATION OF BIOLOGICAL MATERIALS IN A TEST SAMPLE 审中-公开
标题翻译：测试介质和定量或定性方法，用于在测试样品中鉴定和鉴定生物材料
公开(公告)号：WO2006107583A1
公开(公告)日：2006-10-12
申请号：PCT/US2006/010177
申请日：2006-03-21
申请人： MICROLOGY LABORATORIES, LLC , ROTH, Geoffrey, N. , ROTH, Jonathan, N.
发明人： ROTH, Geoffrey, N. , ROTH, Jonathan, N.
IPC分类号： C12Q1/04
CPC分类号： C12Q1/04 , C12Q1/045 , C12Q1/10 , C12Q2334/00 , C12Q2334/50 , G01N2333/245 , G01N2333/25 , G01N2333/255 , G01N2333/924 , Y02A50/451
摘要： A test medium and method for detecting, quantifying, identifying and differentiating up to four (4) separate biological materials in a test sample. A test medium is disclosed which allows quantifying and differentiating under ambient light aggregates of biological entities producing specific enzymes, which might include general coliforms, E. coli, Aeromonas, and Salmonella in a single test medium. A new class of nonchromogenic substrates is disclosed which produce a substantially black, non-diffusible precipitate. This precipitate is not subject to interference from other chromogenic substrates present in the test medium. In one embodiment, the substrates are selected such that E. coli colonies present in the test medium show as substantially black, general coliforms colonies show in the test medium as a blue-violet color, Aeromonas colonies present in the test medium show as a generally red- pink color, and Salmonella colonies show as a generally teal-green color. Other microorganisms and color possibilities for detection and quantification thereof are also disclosed. An inhibitor and method for making a test medium incorporating the inhibitor are disclosed.
摘要翻译：用于在测试样品中检测，定量，鉴定和区分多达四（4）种分离的生物材料的测试介质和方法。公开了一种测试介质，其允许在单个测试介质中产生特定酶的生物实体的环境光聚集体（其可以包括一般大肠杆菌，大肠杆菌，气单胞菌属和沙门氏菌）进行定量和鉴别。公开了一类新的非致冷底物，其产生基本上黑色的非扩散性沉淀物。该沉淀物不受来自测试介质中存在的其它显色底物的干扰。在一个实施方案中，选择底物使得存在于测试培养基中的大肠杆菌菌落显示为基本上黑色，一般大肠菌群在测试培养基中显示为蓝紫色，测试培养基中存在的气单胞菌菌落显示为一般红色粉红色和沙门氏菌菌落呈普通绿绿色。还公开了用于其检测和定量的其它微生物和颜色可能性。公开了一种制备包含抑制剂的测试介质的抑制剂和方法。

3. 发明申请

WO0109372B1 CHEMILUMINESCENT SUBSTRATES OF HYDROLYTIC ENZYMES SUCH AS PHOSPHATASES 审中-公开
标题翻译：水解酶如CHPHUMLESETIC基质如磷酸盐
公开(公告)号：WO0109372B1
公开(公告)日：2001-06-14
申请号：PCT/US0020429
申请日：2000-07-27
申请人： BAYER AG , JIANG QINGPING , NATRAJAN ANAND , SHARPE DAVID J , WONG WEN JEE , LAW SAY JONG
发明人： JIANG QINGPING , NATRAJAN ANAND , SHARPE DAVID J , WONG WEN-JEE , LAW SAY-JONG
IPC分类号： G01N21/78 , C07D219/04 , C07D491/10 , C07F9/64 , C09K11/07 , C12M1/34 , C12Q1/34 , C12Q1/42 , C07D219/06
CPC分类号： C07D491/10 , C07D219/04 , C07F9/64 , C12Q1/42 , C12Q2334/00
摘要： Chemiluminescent substrates of hydrolytic enzymes are disclosed having general Formula Lumi-M-P, where Lumi is a chemiluminescent moiety capable of producing light (a) by itself, (b) with MP attached and (c) with M attached, wherein the different properties of Lumi-M-P and Lumi-M allow them to be distinguished. Lumi includes, but is not limited to, acridinium compounds (e.g. acridinium esters, carboxyamides, thioesters and oxime esters), reduced forms thereof (e.g. acridans), and spiroacridan compounds. M is selected from oxygen, nitrogen and sulfur. P is a group that can be readily removed by hydrolytic enzymes to give Lumi-M and P. The hydrolytic enzyme can be phosphatase, glycosidase, peptidase, protease, esterase, sulfatase and guanidinobenzoatase.
摘要翻译：公开了水解酶的化学发光底物，其具有通式Lumi-MP，其中Lumi是能够自身产生光（a）的化学发光部分，（b）MP附着和（c）与M连接，其中Lumi的不同性质 -MP和Lumi-M允许他们被区别。 Lumi包括但不限于吖啶鎓化合物（例如吖啶酯，羧酰胺，硫酯和肟酯），还原形式（例如吖啶）和螺吡啶化合物。 M选自氧，氮和硫。 P是可以通过水解酶容易地除去得到Lumi-M和P的基团。水解酶可以是磷酸酶，糖苷酶，肽酶，蛋白酶，酯酶，硫酸酯酶和胍基苯甲酰化酶。

4. 发明申请

WO00055167A1 2-PYRIDINETHIOL DERIVATIVES AND REAGENTS FOR ACTIVITY MEASUREMENT OF NAG CONTAINING THE SAME 审中-公开
标题翻译： 2-PYRIDINETHIOL衍生物和试剂用于含有其的NAG活性测定
公开(公告)号：WO00055167A1
公开(公告)日：2000-09-21
申请号：PCT/JP2000/001222
申请日：2000-03-02
IPC分类号： C07H17/02 , C12Q1/34
CPC分类号： C07H17/02 , C12Q1/34 , C12Q2334/00 , G01N2333/924
摘要： Compounds represented by general formula (I) and reagents for activity measurement of N-acetylglucosaminidase (NAG) containing the same, wherein R is hydrogen, lower alkoxy, or NHCOCH3; and R is lower alkoxy or NHCOCH3.
摘要翻译：由通式（I）表示的化合物和含有该化合物的N-乙酰氨基葡糖苷酶（NAG）的活性测定试剂，其中R 1为氢，低级烷氧基或NHCOCH 3; R 2是低级烷氧基或NHCOCH 3。

5. 发明申请

WO99050444A1 REAGENT COMPOSITIONS FOR ASSAYING ELECTROLYTES 审中-公开
标题翻译：用于测定电解质的试剂组合物
公开(公告)号：WO99050444A1
公开(公告)日：1999-10-07
申请号：PCT/JP1999/001209
申请日：1999-03-11
IPC分类号： C12Q1/40 , G01N31/22 , G01N33/84
CPC分类号： G01N33/84 , C12Q1/40 , C12Q2334/00 , C12Q2334/10 , G01N31/22 , G01N2400/18 , G01N2800/04
摘要： Reagent compositions for enzymatically assaying electrolytes (calcium ion, chlorine ion, etc.) which withstand as liquid reagents during distribution, have a high solution stability and are excellent in quantitative characteristics and accuracy. These compositions comprises: (a) inactivated alpha -amylase; (b) a chelating agent; (c) an alpha -amylase substrate; and (d) a cyclodextrin derivative; optionally together with an SH-group containing compound or its salt.
摘要翻译：用于酶分析用于分布时作为液体试剂的电解质（钙离子，氯离子等）的试剂组合物具有高溶液稳定性并且在定量特性和精度方面优异。这些组合物包括：（a）灭活的α-淀粉酶; （b）螯合剂; （c）α-淀粉酶底物; 和（d）环糊精衍生物; 任选地与含SH-基团的化合物或其盐一起。

6. 发明申请

WO99040161A1 CHEMILUMINESCENT REACTIONS USING DIHYDROXYAROMATIC COMPOUNDS AND HETEROCYCLIC ENOL PHOSPHATES 审中-公开
标题翻译：使用二羟基芳族化合物和杂环化合物磷酸酯的亮氨酸反应
公开(公告)号：WO99040161A1
公开(公告)日：1999-08-12
申请号：PCT/US1998/011489
申请日：1998-06-12
IPC分类号： G01N33/532 , C07F9/12 , C07F9/64 , C09K3/00 , C09K11/07 , C12Q1/34 , G01N21/78 , G01N33/533 , C07F9/06 , C12Q1/00
CPC分类号： C09K3/00 , C09K11/07 , C12Q1/34 , C12Q2334/00 , G01N33/533
摘要： Novel methods and compositions which generate chemiluminescence are provided. The compositions comprise a heterocyclic enol phosphate compound and a dihydroxyaromatic compound in which the two hydroxy groups are separated by an even number of ring carbon atoms. Novel methods and compositions for generating chemiluminescence by reaction with a hydrolytic enzyme are provided as well. The compositions comprise a heterocyclic enol phosphate compound and a protected dihydroxyaromatic compound in which one of the hydroxy groups of the dihydroxyaromatic compound is protected with an enzyme-cleavable group. The novel chemiluminescent compositions are useful in methods for producing chemiluminescence for use in assays of hydrolytic enzymes and enzyme inhibitors and in assays employing labeled specific binding pairs.
摘要翻译：提供了产生化学发光的新方法和组合物。组合物包含杂环烯醇化合物和二羟基芳族化合物，其中两个羟基被偶数个环碳原子分开。还提供了通过与水解酶反应产生化学发光的新方法和组合物。组合物包含杂环烯醇磷酸酯化合物和被二羟基芳族化合物保护的二羟基芳族化合物，其中二羟基芳族化合物的一个羟基被酶切割基团保护。新型化学发光组合物可用于生产化学发光的方法，用于水解酶和酶抑制剂的测定和使用标记的特异性结合对的测定中。

7. 发明申请

WO1997026245A1 COMPOUNDS, COMPOSITIONS AND METHODS FOR GENERATING CHEMILUMINESCENCE WITH PHOSPHATASE ENZYMES 审中-公开
标题翻译：化合物，组合物和用于产生磷酸酶酶的发光的方法
公开(公告)号：WO1997026245A1
公开(公告)日：1997-07-24
申请号：PCT/US1997000015
申请日：1997-01-15
申请人： LUMIGEN, INC. , AKHAVAN-TAFTI, Hashem , ARGHAVANI, Zahra , DESILVA, Renuka
发明人： LUMIGEN, INC.
IPC分类号： C07D219/00
CPC分类号： C07F9/6541 , C07F9/5765 , C07F9/64 , C12Q1/42 , C12Q2334/00 , Y10S435/968
摘要： Novel heterocyclic compounds which generate chemiluminescence on reaction with a phosphatase enzyme are provided as well as a process for their preparation and intermediates useful therein. The compounds comprise a nitrogen, oxygen or sulfur-containing heterocyclic ring system bearing an exocyclic carbon-carbon double bond. The double bond is further substituted at the distal carbon with a phosphate group and an oxygen or sulfur atom-containing group. Novel compositions further comprising a cationic aromatic compound (CAC) in addition to the heterocyclic phosphate compound are provided. The addition of the CAC in the composition greatly increases the production of chemiluminescence and provides improved detection sensitivity. Compositions further comprising an anionic surfactant and a non-ionic surfactant provide additional improvements in detection sensitivity. The novel chemiluminescent compounds and compositions are useful in methods for producing light and in assays for phosphatase enzymes and enzyme inhibitors and in assays employing enzyme-labeled specific binding pairs.
摘要翻译：提供了与磷酸酶反应产生化学发光的新型杂环化合物以及其制备方法和可用于其中的中间体。该化合物包含带有环外碳 - 碳双键的氮，氧或含硫杂环体系。该双键在远端碳被磷酸基团和含氧或含硫原子的基团进一步取代。提供除了杂环磷酸酯化合物之外还包含阳离子芳族化合物（CAC）的新型组合物。在组合物中添加CAC大大增加了化学发光的产生并提供了改善的检测灵敏度。进一步包含阴离子表面活性剂和非离子表面活性剂的组合物提供检测灵敏度的另外的改进。新颖的化学发光化合物和组合物可用于生产光的方法和用于磷酸酶和酶抑制剂的测定和使用酶标记的特异性结合对的测定中。

8. 发明申请

WO1996030543A1 METHOD AND CULTURE MEDIUM FOR IDENTIFICATION OF SALMONELLAE 审中-公开
标题翻译：用于鉴定沙门氏菌的方法和培养基
公开(公告)号：WO1996030543A1
公开(公告)日：1996-10-03
申请号：PCT/FI1996000163
申请日：1996-03-20
申请人： ORION-YHTYMÄ OY , TUOMPO, Helena , SCHEININ, Leena , JUSSILA, Marita , LAINE, Irmeli
发明人： ORION-YHTYMÄ OY
IPC分类号： C12Q01/10
CPC分类号： C12Q1/10 , C12Q1/045 , C12Q2334/00 , G01N2333/255 , Y10S435/822 , Y10S435/879 , Y10S435/968
摘要： The culture medium and method for distinguishing bacteria of Salmonella spp. from other gram-negative bacteria, especially those belonging to the family Enterobacteriaceae, is based on the ability of salmonellae to utilize melibiose, mannitol, and sorbitol into acids. This property is made use of together with a chromogenic substrate used for identifying beta -galactosidase. Other bacteria of the family Enterobacteriaceae, most of which are beta -galactosidase-positive, appear as brown, blue, or green colonies, depending on the chromogenic substrate used. Apart from Salmonella spp., other beta -galactosidase-negative bacteria, such as Proteus spp., appear as colorless colonies. Salmonellae can be identified directly on the culture medium after incubation, on the basis of their characteristic bright red color.
摘要翻译：用于区分沙门氏菌属细菌的培养基和方法来自其他革兰氏阴性细菌，特别是属于肠杆菌科的革兰氏阴性菌基于沙门氏菌将蜜二糖，甘露醇和山梨醇用于酸中的能力。该性质与用于鉴定β-半乳糖苷酶的显色底物一起使用。其中大部分为β-半乳糖苷酶阳性的肠杆菌科的其他细菌显示为棕色，蓝色或绿色菌落，这取决于所用的显色底物。除沙门氏菌外，其他β-半乳糖苷酶阴性细菌，如变形杆菌属（Proteus spp。），呈现无色菌落。根据其特征亮红色可以在孵育后直接在培养基上鉴定沙门氏菌。

9. 发明申请

WO1995031724A1 PARACETAMOL PHOSPHATE FOR IMMUNOASSAYS 审中-公开
标题翻译：邻苯二甲酸磷酸盐
公开(公告)号：WO1995031724A1
公开(公告)日：1995-11-23
申请号：PCT/EP1995001814
申请日：1995-05-12
申请人： CAMBRIDGE LIFE SCIENCES PLC , ATHEY, Dale , RAWSON, Keith
发明人： CAMBRIDGE LIFE SCIENCES PLC
IPC分类号： G01N33/535
CPC分类号： C12Q1/42 , C12Q2334/00
摘要： A novel method of electrochemical detection of phosphate activity is revealed wherein 4-acetamidophenyl phosphate and N-hydroxy-4-acetamidophenyl phosphate are used as substrates. The substrates are easy to prepare, offer good stability as a powder and in solution. Especially advantageous is the fact, that the hydrolysis products do not impair the surface activity of the measuring electrode, thus offering extended analytical range in assay/immunoassay applications.
摘要翻译：揭示了磷酸活性的电化学检测的新方法，其中使用4-乙酰氨基苯基磷酸酯和N-羟基-4-乙酰氨基苯基磷酸酯作为底物。基材易于制备，作为粉末和溶液提供良好的稳定性。特别有利的是，水解产物不会损害测量电极的表面活性，从而在测定/免疫测定应用中提供扩展的分析范围。

10. 发明申请

WO1994026726A1 IMPROVED CHEMILUMINESCENT 1,2-DIOXETANES 审中-公开
标题翻译：改进的亮氨酸1,2-二氧化物
公开(公告)号：WO1994026726A1
公开(公告)日：1994-11-24
申请号：PCT/US1994004555
申请日：1994-05-06
申请人： TROPIX, INC.
发明人： TROPIX, INC. , BRONSTEIN, Irena , EDWARDS, Brooks , SPARKS, Alison
IPC分类号： C07D265/00
CPC分类号： G01N33/582 , A43B1/0036 , A43B1/0072 , A43B3/0031 , C07C43/1788 , C07C43/196 , C07C43/215 , C07C43/225 , C07C43/23 , C07C43/315 , C07C45/673 , C07C47/565 , C07C47/575 , C07C65/03 , C07C65/21 , C07C205/37 , C07C205/44 , C07C217/84 , C07C233/18 , C07C271/28 , C07C271/58 , C07C2603/74 , C07D321/00 , C07F9/12 , C07F9/4056 , C07F9/65512 , C07F9/65742 , C07G3/00 , C07H15/203 , C07H15/26 , C12Q1/34 , C12Q1/37 , C12Q1/42 , C12Q2334/00 , G01N21/76 , G01N33/581 , Y10S435/81 , Y10S435/975
摘要： Novel 1,2-dioxetanes with improved chemiluminescent properties, such as signal intensity, S/N ratio, T1/2, etc. are provided by spiroadamantyl 1,2-dioxetanes, wherein the remaining carbon atom of the ring bears an alkoxy, aryloxy, or arylalkoxy substituent, and either a phenyl or naphthyl ring, this aromatic ring bearing, at the meta position on the phenyl group, or a non-conjugated position on the naphthyl ring, an OX moiety wherein X is an enzyme-clevable group, which when removed from the dioxetane, leaves the oxyanion which decomposes with chemiluminescence, the aryl ring further bearing an electron-active substituent Z. The nature and placement of the Z substituent, at a position not adjacent the point of attachment to the dioxetane ring, strongly influences the properties of the dioxetane. Assays, as well as kits for the performance of those assays, include the dioxetane, an enzyme capable of cleaving the X group, and in certain cases, membranes and chemiluminescent enhancement agents.
摘要翻译：具有改善的化学发光性能的新型1,2-二氧杂环丁烷，如信号强度，S / N比，T1 / 2等，由金刚烷基1,2-二氧杂环丁烷提供，其中环的剩余碳原子为烷氧基，芳氧基或芳基烷氧基取代基，苯基或萘基环，该芳环在苯基间位，或在萘环上的非结合位置，O X部分，其中X是可酶解的基团，当从二氧杂环丁烷中除去时，离开化学发光分解的氧阴离子，芳基还具有电子活性取代基Z.Z-取代基在不与二氧杂环丁烷环的连接点相邻的位置处的性质和位置，强烈地影响二氧杂环丁烷的性质。测定以及用于进行这些测定的试剂盒包括二氧杂环丁烷，能够切割X基团的酶，以及在某些情况下，包括膜和化学发光增强剂。

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