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1. 发明申请

WO1996019570A1 AN ENZYME PREPARATION WITH CELLULYTIC ACTIVITY 审中-公开
标题翻译：具有细胞活性的酶的制备
公开(公告)号：WO1996019570A1
公开(公告)日：1996-06-27
申请号：PCT/DK1995000518
申请日：1995-12-22
申请人： NOVO NORDISK A/S , LANGE, Lene , NIELSEN, Jack, Bech , SCHÜLEIN, Martin
发明人： NOVO NORDISK A/S
IPC分类号： C12N09/42
CPC分类号： D21H21/10 , C11D3/38645 , D06M16/003 , D06P5/02 , D06P5/158 , D21C5/005 , D21C5/027 , D21H17/005 , Y02W30/648
摘要： A detergent composition comprising an enzyme preparation with substantial cellulytic activity, especially endoglucanase activity, at alkaline conditions which enzyme composition is derived from or producible by a fungus selected from the Basidiomycetous families Coprinaceae and Bolbitiaceae, preferably from the group of strains belonging to the genera Psathyrella, Podaxis, Panaeolus, Coprinus and Bolbitius and a method for providing a stone-washed look to textiles, especially to cellulosic fabric such as denim, using the mentioned enzyme preparation; and a method for improving the drainage of paper pulp or the de-inking of recycled paper using the mentioned enzyme preparation; and an enzyme preparation with substantial cellulytic activity at alkaline conditions which enzyme composition is derived from or producible by a fungus selected from the Basidiomycetous families Coprinaceae and Bolbitiaceae, provided that the fungus does not belong to the species Coprinus cinereus.
摘要翻译：一种洗涤剂组合物，其包含在碱性条件下具有显着的纤维素分解活性，特别是内切葡聚糖酶活性的酶制剂，所述酶组合物来自或可由选自巴斯德氏阴茎科家蝇科和科博比亚科的真菌产生，优选来自属于Psathyrella属的组，Podaxis，Panaeolus，Coprinus和Bolbitius，以及使用所述酶制剂向纺织品，特别是纤维素织物如牛仔布提供石洗的外观的方法; 以及使用所述酶制剂改进纸浆排出或再循环纸的脱墨的方法; 以及在碱性条件下具有显着的纤维素分解活性的酶制剂，其中酶组成来源于选自巴斯德氏酵母属科罗班菌科和博比氏菌科的真菌，或者可以由真菌产生，只要该真菌不属于灰霉属（Coprinus cinereus）。

2. 发明申请

WO1996007324A1 CLEANING, DISINFECTING AND PRESERVING CONTACT LENSES 审中-公开
标题翻译：清洁，消毒和保存接触镜
公开(公告)号：WO1996007324A1
公开(公告)日：1996-03-14
申请号：PCT/DK1995000363
申请日：1995-09-11
申请人： NOVO NORDISK A/S , AASLYNG, Dorrit , NIELSEN, Jack, Bech , KIERSTEIN NIELSEN, Lone
发明人： NOVO NORDISK A/S
IPC分类号： A01N63/00
CPC分类号： A61L12/082
摘要： The invention relates to a method of cleaning, disinfecting and preserving contact lenses comprising treating contact lenses in the following order with: 1) at least one enzyme, 2) at least one enzyme inhibitor, 3) at least one disinfecting agent, and 4) optionally rinsing in e.g. physiological saline solution. A contact lens product, a tablet or capsule for cleaning, disinfecting and preserving contact lenses, and the use of an enzyme inhibitor are also disclosed.
摘要翻译：本发明涉及一种清洁，消毒和保存隐形眼镜的方法，包括以下列顺序处理隐形眼镜：1）至少一种酶，2）至少一种酶抑制剂，3）至少一种消毒剂，和4）任选地在例如生理盐水溶液。还公开了用于清洁，消毒和保存隐形眼镜的隐形眼镜产品，片剂或胶囊以及使用酶抑制剂。

3. 发明申请

WO1994019528A1 A PROCESS FOR PROVIDING LOCALIZED VARIATION IN THE COLOUR DENSITY OF FABRICS 审中-公开
标题翻译：提供织物颜色密度局部变化的过程
公开(公告)号：WO1994019528A1
公开(公告)日：1994-09-01
申请号：PCT/DK1994000078
申请日：1994-02-25
申请人： NIELSEN, Jack, Bech
IPC分类号： D06P05/00
CPC分类号： D06P1/5257 , D06P1/525 , D06P1/647 , D06P5/158
摘要： Process for providing improved localised variation in the colour density of the surface of dyed fabrics, the process comprising treating a dyed fabric with a cellulytic enzyme in an aqueous liquor comprising a di- or trivalent cation and a chelating agent in a molar ratio of 1:0.1-50.
摘要翻译：用于提供染色织物表面的颜色密度的改进的局部变化的方法，所述方法包括在含有摩尔比为1：1的二价或三价阳离子和螯合剂的水溶液中用纤维素分解酶处理染色织物， 0.1-50。

4. 发明申请

WO2003024242A1 ENZYMATIC TREATMENT OF STARCHY FOOD PRODUCTS FOR SHORTENING THE TEMPERING STEP 审中-公开
标题翻译：用于冷冻温度步骤的食品的酶解处理
公开(公告)号：WO2003024242A1
公开(公告)日：2003-03-27
申请号：PCT/DK2002/000596
申请日：2002-09-12
申请人： NOVOZYMES A/S , SPENDLER, Tina , NIELSEN, Jack, Bech
发明人： SPENDLER, Tina , NIELSEN, Jack, Bech
IPC分类号： A23L1/105
CPC分类号： A23L7/165 , A21D8/04 , A21D13/50 , A23L7/107 , A23L7/126 , A23L7/13 , C12Y302/01001 , C12Y302/01041
摘要： The addition of an endo-amylase or a microbial pullulanase to starch-based raw materials accelerates the retrogradation and thus allows a shortening of the holding period. A process for producing an edible product comprises mixing an endo-amylase or a microbial pullulanase with raw materials comprising starch, heating so as to gelatinize the starch, cooling, holding to effect retrogradation of the starch, and heating.
摘要翻译：将内切淀粉酶或微生物支链淀粉酶添加到基于淀粉的原料中加速了退化，从而缩短了保存期。生产可食用产品的方法包括将内切淀粉酶或微生物支链淀粉酶与包含淀粉的原料混合，加热以使淀粉糊化，冷却，保持淀粉的淀粉和加热。

5. 发明申请

WO1995024471A1 NOVEL ALKALINE CELLULASES 审中-公开
标题翻译：新的碱性细胞
公开(公告)号：WO1995024471A1
公开(公告)日：1995-09-14
申请号：PCT/DK1995000108
申请日：1995-03-08
申请人： NOVO NORDISK A/S , SCHÜLEIN, Martin , ROSHOLM, Peter , NIELSEN, Jack, Bech , HANSEN, Svend, Aage , VON DER OSTEN, Claus
发明人： NOVO NORDISK A/S
IPC分类号： C12N09/42
CPC分类号： C12N9/2437 , C11D3/386 , C11D3/38645 , D06L4/40 , D06M16/003 , D06P5/02 , D06P5/137 , D06P5/158 , D21C5/005 , D21C9/005 , D21H17/005 , D21H21/10
摘要： Cellulases selected from the group consisting of Family 7 cellulases and variants of these cellulases comprising a core and optionally a C-terminal link consisting of 10 amino acids at the most, especially cellulases having tryptophan, tyrosine or phenylalanine in position 55 relative to the sequence alignment of Figure 5 and/or cellulases having a substrate binding cleft of a depth of at least 12 ANGSTROM , exhibit enhanced enzyme activity in the alkaline pH range while exerting a moderate cellulolytic action on the cellulosic substrate and are, for example, useful in detergent compositions, especially for soil removal or colour clarification or preventing backstaining; in fabric softeners; for bio-polishing of textiles; for drainage improvement of paper pulp; for plant degradation. Cellulases from Humicola insolens, Fusarium oxysporum, Trichoderma reesei and Myceliophthora thermophile are referred to.
摘要翻译：选自由7族纤维素酶和这些纤维素酶变体组成的组的纤维素酶，其包含核心和任选的由最多10个氨基酸组成的C-末端连接物，特别是相对于序列比对在位置55具有色氨酸，酪氨酸或苯丙氨酸的纤维素酶和/或具有至少12 ANGSTROM深度的底物结合裂缝的纤维素酶在碱性pH范围内表现出增强的酶活性，同时在纤维素基质上施加中等的纤维素分解作用，并且例如可用于洗涤剂组合物，特别是去除污垢或进行颜色澄清或防止背染; 织物柔软剂; 用于纺织品的生物抛光; 用于纸浆排水改良; 用于植物降解。来自Humicola insolens，尖孢镰刀菌，里氏木霉和嗜热丝孢杆菌的纤维素酶被参考。

6. 发明申请

WO1997001629A1 A CELLULASE WITH REDUCED MOBILITY 审中-公开
标题翻译：具有降低移动性的纤维素酶
公开(公告)号：WO1997001629A1
公开(公告)日：1997-01-16
申请号：PCT/DK1996000284
申请日：1996-06-26
申请人： NOVO NORDISK A/S , NIELSEN, Jack, Bech , TIKHOMIROV, Dmitry Feodorovich
发明人： NOVO NORDISK A/S
IPC分类号： C12N09/42
CPC分类号： C12N9/2437 , C11D3/38645 , C12Y302/01004 , D06M16/003 , D06P5/02 , D06P5/158
摘要： A cellulolytic enzyme preparation comprising a cellulase with reduced mobility, e.g. by increasing the molecular weight or apparent size of the cellulase protein molecule or by insolubilizing or immobilizing the cellulase such as by incorporation of the cellulase component into a gel, by the formation of stable or temporary aggregates with enhanced molecular mass, by rapid immobilization of cellulase protein on insoluble components, by rapid autoimmobilization of the cellulase protein, or by adsorption to an insoluble or soluble carrier, preferably a cellulose-containing carrier of fibrous, microcrystalline or amorphous structure, more preferably a soluble or insoluble polymer, especially a polysaccharide capable of interaction with the enzyme via a cellulose binding domain (CBD) or catalytic domain, or a soluble polycationic cellulose derivative, the cellulase preparation having a much lesser effect or influence on the durability or ageing behaviour of the cellulosic substrate than corresponding unmodified cellulases while at least having as good an effect on the look or feel, when used for treatment of cellulosic fabrics or textiles such as for domestic or industrial laundering or fabric softening as an ingredient of a detergent composition, for bio-polishing or for stone-washing of denim fabric or denim jeans or other dyed fabric or garments.
摘要翻译：包含具有降低迁移率的纤维素酶的纤维素分解酶制剂，例如通过增加纤维素酶蛋白质分子的分子量或表观尺寸，或通过使纤维素酶不溶或固定化，例如通过将纤维素酶组分掺入凝胶中，通过形成具有增强分子量的稳定或临时聚集体，通过快速固定纤维素酶通过纤维素酶蛋白的快速自动修复，或通过吸附到不溶性或可溶性载体，优选纤维状，微晶结晶或无定形结构的纤维素载体，更优选可溶性或不溶性聚合物，特别是能够通过纤维素结合结构域（CBD）或催化结构域或可溶性聚阳离子纤维素衍生物与酶的相互作用，所述纤维素酶制剂对纤维素底物的耐久性或老化行为的影响比对照未修饰的纤维素酶具有更小的影响或影响，而至少对外观有很好的效果或感觉，当用于纤维素织物或纺织品的处理时，例如用于家庭或工业洗涤或织物软化作为洗涤剂组合物的成分，用于生物抛光或用于牛仔布或牛仔布牛仔裤或其他染色织物的石洗涤或服装。

7. 发明申请

WO1996011262A1 AN ENZYME AND ENZYME PREPARATION WITH ENDOGLUCANASE ACTIVITY 审中-公开
标题翻译：使用内切酶活性的酶和酶制备
公开(公告)号：WO1996011262A1
公开(公告)日：1996-04-18
申请号：PCT/DK1995000400
申请日：1995-10-06
申请人： NOVO NORDISK A/S , SCHÜLEIN, Martin , OXENBOLL, Karen, Margrethe , ANDERSEN, Lene, Nonboe , LASSEN, Soren, Flensted , KAUPPINEN, Markus, Sakari , NIELSEN, Jack, Bech
发明人： NOVO NORDISK A/S
IPC分类号： C12N09/42
CPC分类号： D21H17/005 , C11D3/38645 , C12N9/2437 , C12N15/81 , C12Y302/01004 , D06M16/003 , D06P5/158 , D21C5/005 , D21H21/10
摘要： An enzyme composition with substantial cellulolytic, especially endoglucanase, activity at alkaline conditions which is derived from or producible by fungi from the genus Acremonium, provided that the enzyme composition is not immunologically cross-reactive with antibodies raised against endoglucanases (EC 3.2.1.4) from Humicola insolens, DSM 1800, Fusarium oxysporum, DSM 2672, Myceliophthora thermophila, CBS 117.65, or Cephalosporium sp., RYM-202. The invention further relates to a DNA construct comprising a DNA sequence encoding an enzyme exhibiting endoglucanase activity. The enzyme composition and the isolated or cloned and expressed cellulase of the invention may be useful in any industrial process requiring an alkaline cellulase, e.g. for providing localised variation in the colour density of dyed fabric such as stone-washing of denim, for improving the drainage of an aqueous suspension of paper pulp, for the de-inking of recycled paper, in detergent compositions and in fabric softeners.
摘要翻译：在碱性条件下具有显着的纤维素分解，特别是内切葡聚糖酶的酶组合物，其衍生自或者由来自顶孢菌属的真菌产生的活性，条件是所述酶组合物与针对内切葡聚糖酶（EC 3.2.1.4）产生的抗体不具有免疫交叉反应性 Humicola insolens，DSM 1800，尖孢镰刀菌，DSM 2672，嗜热丝孢杆菌，CBS 117.65或头孢菌属，RYM-202。本发明还涉及包含编码表现出内切葡聚糖酶活性的酶的DNA序列的DNA构建体。本发明的酶组合物和分离或克隆和表达的纤维素酶可用于任何需要碱性纤维素酶的工业过程，例如，用于提供染色织物的颜色密度的局部变化，例如牛仔布的石洗，用于改进纸浆的水性悬浮液的排出，用于在再生纸中脱墨，在洗涤剂组合物和织物柔软剂中的排墨。

8. 发明申请

WO1995034842A1 CONTACT LENSES WITH IMMOBILIZED PROTEINS FOR DISINFECTING AND DELIVERING OXYGEN TO THE EYES 审中-公开
标题翻译：接触眼镜，用于消毒和输送氧气到眼睛的固定蛋白质
公开(公告)号：WO1995034842A1
公开(公告)日：1995-12-21
申请号：PCT/DK1995000236
申请日：1995-06-13
申请人： NOVO NORDISK A/S , AASLYNG, Dorrit , NIELSEN, Jack, Bech
发明人： NOVO NORDISK A/S
IPC分类号： G02C07/04
CPC分类号： G02B1/043 , A61L12/126
摘要： The present invention relates to a contact lens product for extended wear, comprising: 1) a contact lens, permeable to certain oxygen-containing compounds, 2) at least one, on the outer surface of the contact lens, immobilized protein, capable of producing an oxygen-containing compound, and 3) at least one, on the inner surface of the contact lens, immobilized protein or catalyst, capable of producing O2 from certain oxygen containing compounds. Further, the process for producing said contact lenses and a method for disinfecting a contact lens and simultaneously delivering oxygen to the eye.
摘要翻译：隐形眼镜产品技术领域本发明涉及一种用于延长磨损的隐形眼镜产品，其特征在于，包括：1）可透过某些含氧化合物的隐形眼镜，2）在隐形眼镜的外表面上至少有一个能够产生含氧化合物，和3）在隐形眼镜的内表面上，能够从某些含氧化合物产生O 2的固定化蛋白质或催化剂中的至少一种。此外，制造所述隐形眼镜的方法和用于消除隐形眼镜并同时将氧气输送到眼睛的方法。

9. 发明申请

WO1995002044A1 AN ENZYME WITH PROTEASE ACTIVITY 审中-公开
标题翻译：具有蛋白酶活性的酶
公开(公告)号：WO1995002044A1
公开(公告)日：1995-01-19
申请号：PCT/DK1994000274
申请日：1994-07-05
申请人： NOVO NORDISK A/S , DALBOGE, Henrik , CHRISTGAU, Stephan , ANDERSEN, Lene, Nonboe , KOFOD, Lene, Venke , KAUPPINEN, Markus, Sakari , NIELSEN, Jack, Bech , DAMBMANN, Claus
发明人： NOVO NORDISK A/S
IPC分类号： C12N09/62
CPC分类号： C11D3/3947 , A21D8/042 , A23K20/189 , C11D3/0078 , C11D3/386 , C11D3/38618 , C11D3/38636 , C12N9/62 , C12N9/82
摘要： A DNA construct comprising a DNA sequence encoding an enzyme exhibiting protease activity, which DNA sequence comprises the DNA sequence shown in SEQ ID No. 1 or 2 or an analogue of any of these sequences being at least 80 % homologous to the DNA sequence shown in SEQ ID No. 1 or 2. The proteases encoded by the DNA sequences have an acid pH optimum.
摘要翻译：包含编码表现出蛋白酶活性的酶的DNA序列的DNA构建体，所述DNA序列包含SEQ ID No.1或2所示的DNA序列或这些序列中的任一个的类似物与所示的DNA序列至少80％同源 SEQ ID No.1或2.由DNA序列编码的蛋白酶具有酸性pH最佳值。

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