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    • 7. 发明申请
    • SACCHAROMYCES CEREVISIAE STRAINS
    • SACCHAROMYCES CEREISIAE STRAINS
    • WO2015163814A1
    • 2015-10-29
    • PCT/SE2015/050456
    • 2015-04-23
    • SCANDINAVIAN TECHNOLOGY GROUP AB
    • BONANDER, Nicklas
    • C12N15/04C12P7/06C12N1/18C12R1/865
    • C12N1/18C12N9/0006C12N9/1205C12P7/10C12R1/865C12Y101/01009C12Y101/01307C12Y207/01017Y02E50/16Y02E50/17
    • The present invention relates to a method of preparing a strain of sugar fermenting S accharomyces cerevisiae with capability to ferment xylose, wherein said method comprises different procedural steps. The method comprises mating a first sporulated Saccharomyces cerevisiae strain with a second Saccharomyces cerevisiae haploid strain. Thereafter, screening for mated cells is performed, growing such mated cells, and verifying that mated cells exhibit basic morphology by microscopic inspection. Thereafter, creation of a mixture of the mated cells is performed, subjecting the mixture to continuous chemostat lignocellulose cultivation and obtaining the sugar fermenting Saccharomyces cerevisiae cells with capability to ferment xylose is performed. The invention also comprises strains obtained by said method.
    • 本发明涉及一种制备具有发酵木糖能力的糖酵母酿酒酵母菌株的方法,其中所述方法包括不同的程序步骤。 该方法包括将第一种孢子形成的酿酒酵母菌株与第二种酿酒酵母单倍体菌株交配。 此后,进行配对细胞的筛选,生长这样的配对细胞,并通过显微镜检查验证配对细胞呈现基本形态。 然后,进行配合的细胞的混合物的制作,对混合物进行连续的恒化木素纤维素的培养,并且获得具有发酵木糖能力的糖发酵酿酒酵母细胞。 本发明还包括通过所述方法获得的菌株。