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    • 4. 发明申请
    • COMPOSITIONS AND METHODS FOR IDENTIFYING AND DIFFERENTIATING VIRAL COMPONENTS OF MULTIVALENT SHIPPING FEVER VACCINES
    • 用于识别和分化多种运输费用疫苗的病毒组分的组合物和方法
    • WO2012092054A3
    • 2012-12-27
    • PCT/US2011066392
    • 2011-12-21
    • LILLY CO ELIWEISE DALE WADEHARRIS JAMES ROBERT
    • WEISE DALE WADEHARRIS JAMES ROBERT
    • C12Q1/70
    • C12Q1/70C12Q1/701
    • Disclosed are methods and compositions for identifying viral-specific polynucleotide sequences in a biological sample, and particularly in samples of veterinary origin. Also disclosed are oligonucleotide primer pairs, as well as labeled oligonucleotide detection probes useful in detecting the presence of one or more particular species, strains, types, or subtypes of one or more mammalian pathogens of viral origin, as well as systems, diagnostic kits and articles of manufacture comprising virus-specific primers and labeled detection probes, including those useful in identifying viral components of a multivalent vaccine, and quantitating the potency of particular attenuated, live viruses that comprise a livestock vaccine. In certain embodiments, real-time, quantitative PCR methods have been utilized to identify and distinguish between the three known genetic subtypes of bovine viral diarrhea virsuses (BVDV-la, BVDV-lb, and BVDV-2) in a multivalent bovid shipping fever vaccine.
    • 公开了用于鉴定生物样品中特别是兽类来源的样​​品中的病毒特异性多核苷酸序列的方法和组合物。 还公开了寡核苷酸引物对,以及可用于检测病毒来源的一种或多种哺乳动物病原体的一种或多种特定物种,菌株,类型或亚型的存在的标记的寡核苷酸检测探针,以及系统,诊断试剂盒和 包括病毒特异性引物和标记的检测探针的制品,包括用于鉴定多价疫苗的病毒组分的那些,以及定量包含家畜疫苗的特定减毒活病毒的效力。 在某些实施方案中,已经使用实时定量PCR方法来鉴定和区分多价bovid运输发热疫苗中三种已知的牛病毒性腹泻病毒(BVDV-1a,BVDV-1b和BVDV-2)的遗传亚型 。