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1. 发明申请

WO2007071365A1 CONTROLS FOR NUCLEIC ACID TESTING 审中-公开
标题翻译：核酸测试控制
公开(公告)号：WO2007071365A1
公开(公告)日：2007-06-28
申请号：PCT/EP2006/012215
申请日：2006-12-19
申请人： ROCHE DIAGNOSTICS GMBH , F. HOFFMANN-LA ROCHE AG , EMRICH, Thomas , HABERHAUSEN, Gerd , MOCZKO, Martin
发明人： EMRICH, Thomas , HABERHAUSEN, Gerd , MOCZKO, Martin
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6844 , C12Q2600/166 , C12Q2545/101
摘要： The present invention is related to a method for detecting a target biomolecule in test sample by adding an internal control biomolecule to the test sample; to a negative control sample, to a positive control sample and to a reagent control sample or adding an internal control biomolecule to the test sample, to a negative control sample, to a positive control sample comprising the target biomolecule and providing a reagent control sample comprising the target biomolecule, determining in each sample a signal, and verifying the signal thereby detecting the target biomolecule. The invention is also related to a method for verifying the determination of a signal indicating the presence of a target biomolecule. The invention is further related to a method for detecting the presence or the absence of a member of a group of target nucleic acids in a sample and a method for verifying the determination of a signal indicating the presence of a member of a group of target nucleic acids. Uses and kits are considered as well.
摘要翻译：本发明涉及通过向测试样品添加内部控制生物分子来检测测试样品中的目标生物分子的方法; 将阴性对照样品与阳性对照样品和试剂对照样品或者将测试样品的内部对照生物分子加入到阴性对照样品中，加入到包含目标生物分子的阳性对照样品中，并提供试剂对照样品，其包含目标生物分子，在每个样本中确定信号，并验证信号从而检测目标生物分子。本发明还涉及用于验证指示存在目标生物分子的信号的确定的方法。本发明还涉及用于检测样品中一组靶核酸成员的存在或不存在的方法以及用于验证指示靶核酸组成员的信号的确定的方法酸。用途和套件也被考虑。

2. 发明申请

WO9923250A3 SPECIFIC AND SENSITIVE METHOD FOR DETECTING NUCLEIC ACIDS 审中-公开
标题翻译：特异性和敏感的核酸检测程序
公开(公告)号：WO9923250A3
公开(公告)日：1999-07-22
申请号：PCT/EP9806961
申请日：1998-11-03
申请人： ROCHE DIAGNOSTICS GMBH , KESSLER CHRISTOPH , HABERHAUSEN GERD , BARTL KNUT , ORUM HENRIK
发明人： KESSLER CHRISTOPH , HABERHAUSEN GERD , BARTL KNUT , ORUM HENRIK
IPC分类号： C12N15/09 , C12Q1/68
CPC分类号： C12Q1/6823 , C12Q1/6818 , C12Q1/686 , C12Q2561/101
摘要： The invention relates to a method for detecting a nucleic acid, comprising the following steps: the production of a number of enhancers of a fragment of said nucleic acid with a length of less than 100 nucleotides using two primers, one of said primers being able to bind to a first binding sequence (A) of a strand of the nucleic acid and the other primer being able to bind to a second binding sequence (C') which is essentially complementary to a sequence (C), said sequence (C) not overlapping with (A) and being positioned in the direction 3' from A, in the presence of a probe with a binding sequence (D) which is able to bind to the third sequence (B) positioned between the sequences (A) and (C) or the complement (B') thereof, said probe containing a reporter group and a quencher group, using a polymerase with 5' nuclease activity; and detection of the nucleic acid through the measurement of a signal which is determined by the release of the reporter group.
摘要翻译：本发明提供一种方法，用于检测的核酸，其包括制备多个所述核酸使用两种引物，其中一个与所述核酸的一条链的第一结合序列（A）结合一个小于100个核苷酸的长度的一部分的扩增子的步骤这些都可以可在其它结合第二结合序列（C“），该（C）是实质上以非重叠而与（a）中，位于3”选自（a）序列方向是互补的，在一个存在用的结合序列（d），该第三序列位于所述序列之间的（a）和（C）（B）或（B“）的互补探针可在其结合，用含报告基团和淬灭剂基团，所述探针，使用具有5'核酸酶活性的聚合酶并通过测量由于报道基团释放引起的信号来检测核酸。

3. 发明申请

WO2006035062A1 DETECTION, IDENTIFICATION AND DIFFERENTIATION OF SERRATIA SPECIES USING THE SPACER REGION 审中-公开
标题翻译：使用间隔区域检测，鉴定和分类的SERRATIA物种
公开(公告)号：WO2006035062A1
公开(公告)日：2006-04-06
申请号：PCT/EP2005/054913
申请日：2005-09-29
申请人： INNOGENETICS N.V. , ROCHE DIAGNOSTICS GMBH , JANNES, Geert , MIJS, Wouter , EMRICH, Thomas , HABERHAUSEN, Gerd
发明人： JANNES, Geert , MIJS, Wouter , EMRICH, Thomas , HABERHAUSEN, Gerd
IPC分类号： C12Q1/68
CPC分类号： C12Q1/689 , Y10T436/143333
摘要： The present invention relates to new nucleic acid sequences derived from the ITS (Internal Transcribed Spacer) region, between the 16S and 23 S rRNA genes, to be used for the specific detection and/or identification of Serratia species, in particular of Serratia marcescens, Serratia ficaria and/or Serratia fonticola, in a biological sample. The present invention relates also to a method for the specific detection and/or identification of Serratia species, in particular Serratia marcescens, Serratia ficaria and/or Serratia fonticola, using said new nucleic acid sequences derived from the ITS region. It relates also to nucleic acid primers to be used for the amplification of said spacer region of Serratia species in a sample.
摘要翻译：本发明涉及来自16S和23S rRNA基因之间的ITS（内转录间隔区）的新核酸序列，用于特异性检测和/或鉴定沙雷氏菌属，尤其是粘质沙雷氏菌，在沙门氏菌属和/或沙雷氏菌中，在生物样品中。本发明还涉及使用来自ITS区域的所述新的核酸序列特异性检测和/或鉴定沙雷氏菌属物种，特别是沙雷氏菌属沙雷氏菌属，沙雷氏菌属和/或沙雷氏菌属的方法。它还涉及用于扩增样品中沙雷氏菌属物种的所述间隔区的核酸引物。

4. 发明申请

WO2004053156A1 DETECTION, IDENTIFICATION AND DIFFERENTIATION OF EUBACTERIAL TAXA USING A HYBRIDIZATION ASSAY 审中-公开
标题翻译：使用杂交测定法检测，鉴定和鉴定非特异性毒素
公开(公告)号：WO2004053156A1
公开(公告)日：2004-06-24
申请号：PCT/EP2003/013905
申请日：2003-12-08
申请人： INNOGENETICS N.V. , ROCHE DIAGNOSTICS GMBH , DE HENAU, Hilde , VAN CROMBRUGGEN, Joachim , JANNES, Geert , HABERHAUSEN, Gerd , EMRICH, Thomas
发明人： DE HENAU, Hilde , VAN CROMBRUGGEN, Joachim , JANNES, Geert , HABERHAUSEN, Gerd , EMRICH, Thomas
IPC分类号： C12Q1/68
CPC分类号： C12Q1/689
摘要： The present invention relates to a method for the specific detection and/or identification of Enterococcus species, in particular Enterococcus faecalis and/or Enterococcus faecium, using new nucleic acid sequences derived from the ITS (Internal Transcribed Spacer) region.The present invention relates also to said new nucleic acid sequences derived from the ITS region, between the 16S and 23S ribosomal ribonucleic acid (rRNA) or rRNA genes, to be used for the specific detection and/or identification of Enterococcus species, in particular of Enterococcus faecalis and/or Enterococcus faecium, in a biological sample.It relates also to nucleic acid primers to be used for the amplification of said spacer region of Enterococcus species in a sample.
摘要翻译：本发明涉及使用源自ITS（内转录间隔区）的新核酸序列来特异性检测和/或鉴定肠球菌属物种，特别是粪肠球菌和/或屎肠球菌的方法。本发明还涉及对来自ITS区域的所述新的核酸序列，16S和23S核糖核糖核酸（rRNA）或rRNA基因之间，用于特异性检测和/或鉴定肠球菌属物种，特别是粪肠球菌和/或生物样品中的屎肠球菌。还涉及用于在样品中扩增肠球菌属物种的所述间隔区的核酸引物。

5. 发明申请

WO2005075673A2 DETECTION, IDENTIFICATION AND DIFFERENTIATION OF PROTEUS SPECIES USING THE SPACER REGION 审中-公开
标题翻译：使用间隔区域的物种的检测，鉴定和鉴定
公开(公告)号：WO2005075673A2
公开(公告)日：2005-08-18
申请号：PCT/EP2005/050464
申请日：2005-02-03
申请人： INNOGENETICS N.V. , ROCHE GMBH , JANNES, Geert , MIJS, Wouter , HABERHAUSEN, Gerd , EMRICH, Thomas
发明人： JANNES, Geert , MIJS, Wouter , HABERHAUSEN, Gerd , EMRICH, Thomas
IPC分类号： C12Q1/68
CPC分类号： C12Q1/68 , C12Q1/689
摘要： The present invention relates to new nucleic acid sequences derived from the ITS region, between the 16S and 23S ribosomal ribonucleic acid (rRNA) or rRNA genes, to be used for the specific detection and/or identification of Proteus species, in particular of Proteus mirabilis, Proteus vulgaris and/or Proteus penneri, in a biological sample. The present invention relates also to a method for the specific detection and/or identification of Proteus species, in particular Proteus mirabilis, Proteus vulgaris and/or Proteus penneri, using said new nucleic acid sequences derived from the ITS (Internal Transcribed Spacer) region. It relates also to nucleic acid primers to be used for the amplification of said spacer region of Proteus species in a sample.
摘要翻译：本发明涉及用于特异性检测和/或鉴定变形菌属物种的16S和23S核糖核糖核酸（rRNA）或rRNA基因之间的ITS区域的新核酸序列，特别是变形杆菌，普通变形杆菌（Proteus vulgaris）和/或变形杆菌（Proteus penneri）。本发明还涉及使用衍生自ITS（内部转录间隔物）区域的所述新的核酸序列特异性检测和/或鉴定变形杆菌物种，特别是奇异变形杆菌，普通变形杆菌和/或变形杆菌的方法。它还涉及用于扩增样品中变形杆菌物种的所述间隔区的核酸引物。

6. 发明申请

WO2004053457A2 QUANTITATIVE TEST FOR BACTERIAL PATHOGENS 审中-公开
标题翻译：细菌病原体的定量试验
公开(公告)号：WO2004053457A2
公开(公告)日：2004-06-24
申请号：PCT/US0338783
申请日：2003-12-05
申请人： ROCHE DIAGNOSTICS GMBH , HOFFMANN LA ROCHE , UNIV LELAND STANFORD JUNIOR , BARON ELLEN J , ENGEL WOLF-DIETER , HABERHAUSEN GERD , KASPER PETER , KOGELNIK ANDREAS M
发明人： BARON ELLEN J , ENGEL WOLF-DIETER , HABERHAUSEN GERD , KASPER PETER , KOGELNIK ANDREAS M
IPC分类号： C12P19/34 , C12Q1/68 , G01N
CPC分类号： C12Q1/689 , C12Q1/6851
摘要： The present invention is directed to a method for analyzing the presence of a bacterial pathogen in a clinical sample comprising the steps of (i) at least partially isolating nucleic acid from said sample, characterize d in that said nucleic acid is selected from a group consisting of either total nucleic acid, total DNA or total RNA, (ii) quantifying the amount of nucleic acid comprising a preselected sequence which is specific for said bacterial pathogen, and (iii) determining whether said amount of nucleic acid comprising a preselected sequence which is specific for said bacterial pathogen exceeds a first predetermined cut off value.
摘要翻译：本发明涉及用于分析临床样品中细菌病原体的存在的方法，其包括以下步骤：（i）至少部分地从所述样品中分离核酸，表征为所述核酸选自：总核酸，总DNA或总RNA，（ii）定量包含对所述细菌病原体特异性的预选序列的核酸的量，和（iii）确定所述含有预选序列的核酸的量是否为对所述细菌病原体特异性超过第一预定切断值。

7. 发明申请

WO0029611A3 NOVEL PRIMERS AND PROBES FOR DETECTING HIV 审中-公开
标题翻译：新的引物和探针于检测艾滋病毒
公开(公告)号：WO0029611A3
公开(公告)日：2000-10-26
申请号：PCT/EP9908211
申请日：1999-10-29
申请人： ROCHE DIAGNOSTICS GMBH , HABERHAUSEN GERD , KASPER PIA , KESSLER CHRISTOPH
发明人： HABERHAUSEN GERD , KASPER PIA , KESSLER CHRISTOPH
IPC分类号： G01N33/50 , C12N15/09 , C12Q1/68 , C12Q1/70 , G01N33/566
CPC分类号： C12Q1/703 , C12Q2537/143
摘要： The invention relates to a method for the subtype and/or species-comprehensive detection of HI viruses in a sample while using at least one oligonucleotide which contains at least 10 successive nucleotides from (i) a highly preserved region of the LTR region, of the gag gene or of the pol gene of HIV, (ii) of a corresponding region of another HI virus isolate, (iii) of a corresponding region of a consensus sequence stemming from a plurality of HI virus isolates or of sequences complementary thereto.
摘要翻译：本发明涉及用于对样品中亚型和/或HIV的跨物种检测的方法使用至少一种寡核苷酸，其包含（i）所述gag基因或pol的LTR区域的高度保守区域的至少10个连续核苷酸 HIV，（ⅱ）另一HI病毒分离株的相应区域，基因（iii）由几个病毒始发的相应区域隔离HI共有序列，或互补序列。

8. 发明申请

WO2005075673A3 DETECTION, IDENTIFICATION AND DIFFERENTIATION OF PROTEUS SPECIES USING THE SPACER REGION 审中-公开
标题翻译：利用间隔区域对蛋白质种类的检测，鉴定和分化
公开(公告)号：WO2005075673A3
公开(公告)日：2005-12-01
申请号：PCT/EP2005050464
申请日：2005-02-03
申请人： INNOGENETICS NV , ROCHE GMBH , JANNES GEERT , MIJS WOUTER , HABERHAUSEN GERD , EMRICH THOMAS
发明人： JANNES GEERT , MIJS WOUTER , HABERHAUSEN GERD , EMRICH THOMAS
IPC分类号： C12Q1/68
CPC分类号： C12Q1/68 , C12Q1/689
摘要： The present invention relates to new nucleic acid sequences derived from the ITS region, between the 16S and 23S ribosomal ribonucleic acid (rRNA) or rRNA genes, to be used for the specific detection and/or identification of Proteus species, in particular of Proteus mirabilis, Proteus vulgaris and/or Proteus penneri, in a biological sample. The present invention relates also to a method for the specific detection and/or identification of Proteus species, in particular Proteus mirabilis, Proteus vulgaris and/or Proteus penneri, using said new nucleic acid sequences derived from the ITS (Internal Transcribed Spacer) region. It relates also to nucleic acid primers to be used for the amplification of said spacer region of Proteus species in a sample.
摘要翻译：本发明涉及用于特异性检测和/或鉴定变形杆菌物种，特别是奇异变形杆菌（Proteus mirabilis）的16S和23S核糖体核糖核酸（rRNA）或rRNA基因之间来自ITS区的新核酸序列，普通变形菌（Proteus vulgaris）和/或普通变形菌（Proteus penneri）。本发明还涉及使用衍生自ITS（内部转录间隔区）区域的所述新核酸序列特异性检测和/或鉴定变形杆菌种类，特别是奇异变形杆菌，普通变形杆菌和/或普通变形杆菌的方法。它还涉及用于扩增样品中变形菌物种的间隔区的核酸引物。

9. 发明申请

WO2004052606A3 DETECTION, IDENTIFICATION AND DIFFERENTIATION OF EUBACTERIAL TAXA USING A HYBRIDIZATION ASSAY 审中-公开
标题翻译：使用杂交测定法检测，鉴定和鉴别非特异性TAXA
公开(公告)号：WO2004052606A3
公开(公告)日：2004-07-29
申请号：PCT/EP0313907
申请日：2003-12-08
申请人： INNOGENETICS NV , ROCHE DIAGNOSTICS GMBH , CLAEYS SOFIE , JANNES GEERT , HABERHAUSEN GERD , EMRICH THOMAS , VERDOODT LIA
发明人： CLAEYS SOFIE , JANNES GEERT , HABERHAUSEN GERD , EMRICH THOMAS , VERDOODT LIA
IPC分类号： C12Q1/68
CPC分类号： C12Q1/689
摘要： The present invention relates to a method for the specific detection and/or identification of Staphylococcus species, in particular Staphylococcus aureus, using new nucleic acid sequences derived from the ITS (Internal Transcribed Spacer) region.The present invention relates also to said new nucleic acid sequences derived from the ITS region, between the 16S and 23S ribosomal ribonucleic acid (rRNA) or rRNA genes, to be used for the specific detection and/or identification of Staphylococcus species, in particular of S. aureus, in a biological sample.It relates also to nucleic acid primers to be used for the amplification of said spacer region of Staphylococcus species in a sample.
摘要翻译：本发明涉及使用来自ITS（内转录间隔区）的新核酸序列来特异性检测和/或鉴定葡萄球菌属物种，特别是金黄色葡萄球菌的方法。本发明还涉及所述新的核酸 16S和23S核糖核酸核糖核酸（rRNA）或rRNA基因之间的ITS区序列用于特异性检测和/或鉴定生物样品中金黄色葡萄球菌，特别是金黄色葡萄球菌的特异性检测和/或鉴定。还涉及用于在样品中扩增葡萄球菌物种的所述间隔区的核酸引物。

10. 发明申请

WO2004053457A3 QUANTITATIVE TEST FOR BACTERIAL PATHOGENS 审中-公开
公开(公告)号：WO2004053457A3
公开(公告)日：2004-06-24
申请号：PCT/US2003/038783
申请日：2003-12-05
申请人： ROCHE DIAGNOSTICS GMBH , F. HOFFMAN-LA ROCHE AG , THE BOARD OF TRUSTEES OF THE LELAND STANFORD JUNIOR UNIVERSITY , BARON, Ellen, J. , ENGEL, Wolf-Dieter , HABERHAUSEN, Gerd , KASPER, Peter , KOGELNIK, Andreas, M.
发明人： BARON, Ellen, J. , ENGEL, Wolf-Dieter , HABERHAUSEN, Gerd , KASPER, Peter , KOGELNIK, Andreas, M.
IPC分类号： C12Q1/68
摘要： The present invention is directed to a method for analyzing the presence of a bacterial pathogen in a clinical sample comprising the steps of (i) at least partially isolating nucleic acid from said sample, characterize d in that said nucleic acid is selected from a group consisting of either total nucleic acid, total DNA or total RNA, (ii) quantifying the amount of nucleic acid comprising a preselected sequence which is specific for said bacterial pathogen, and (iii) determining whether said amount of nucleic acid comprising a preselected sequence which is specific for said bacterial pathogen exceeds a first predetermined cut off value.

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