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1. 发明申请

WO2004052606A2 DETECTION, IDENTIFICATION AND DIFFERENTIATION OF EUBACTERIAL TAXA USING A HYBRIDIZATION ASSAY 审中-公开
标题翻译：用杂交试验检测，鉴定和鉴别细菌毒素
公开(公告)号：WO2004052606A2
公开(公告)日：2004-06-24
申请号：PCT/EP2003/013907
申请日：2003-12-08
申请人： INNOGENETICS N.V. , ROCHE DIAGNOSTICS GMBH , CLAEYS, Sofie , JANNES, Geert , HABERHAUSEN, Gerd , EMRICH, Thomas , VERDOODT, Lia
发明人： CLAEYS, Sofie , JANNES, Geert , HABERHAUSEN, Gerd , EMRICH, Thomas , VERDOODT, Lia
IPC分类号： B29B
CPC分类号： C12Q1/689
摘要： The present invention relates to a method for the specific detection and/or identification of Staphylococcus species, in particular Staphylococcus aureus , using new nucleic acid sequences derived from the ITS (Internal Transcribed Spacer) region.The present invention relates also to said new nucleic acid sequences derived from the ITS region, between the 16S and 23S ribosomal ribonucleic acid (rRNA) or rRNA genes, to be used for the specific detection and/or identification of Staphylococcus species, in particular of S. aureus , in a biological sample.It relates also to nucleic acid primers to be used for the amplification of said spacer region of Staphylococcus species in a sample.
摘要翻译：本发明涉及用于特异性检测和/或鉴定葡萄球菌属物种，特别是金黄色葡萄球菌（Staphylococcus iureus）的方法，使用衍生自ITS（内部转录间隔区）区的新核酸序列。本发明还涉及来源于16S和23S核糖体核糖核酸（rRNA）或rRNA基因之间的ITS区的所述新核酸序列，用于特定检测和/或鉴定葡萄球菌属物种，特别是S。它还涉及用于扩增样品中葡萄球菌属种间隔区的核酸引物。

2. 发明申请

WO2005075673A2 DETECTION, IDENTIFICATION AND DIFFERENTIATION OF PROTEUS SPECIES USING THE SPACER REGION 审中-公开
标题翻译：使用间隔区域的物种的检测，鉴定和鉴定
公开(公告)号：WO2005075673A2
公开(公告)日：2005-08-18
申请号：PCT/EP2005/050464
申请日：2005-02-03
申请人： INNOGENETICS N.V. , ROCHE GMBH , JANNES, Geert , MIJS, Wouter , HABERHAUSEN, Gerd , EMRICH, Thomas
发明人： JANNES, Geert , MIJS, Wouter , HABERHAUSEN, Gerd , EMRICH, Thomas
IPC分类号： C12Q1/68
CPC分类号： C12Q1/68 , C12Q1/689
摘要： The present invention relates to new nucleic acid sequences derived from the ITS region, between the 16S and 23S ribosomal ribonucleic acid (rRNA) or rRNA genes, to be used for the specific detection and/or identification of Proteus species, in particular of Proteus mirabilis, Proteus vulgaris and/or Proteus penneri, in a biological sample. The present invention relates also to a method for the specific detection and/or identification of Proteus species, in particular Proteus mirabilis, Proteus vulgaris and/or Proteus penneri, using said new nucleic acid sequences derived from the ITS (Internal Transcribed Spacer) region. It relates also to nucleic acid primers to be used for the amplification of said spacer region of Proteus species in a sample.
摘要翻译：本发明涉及用于特异性检测和/或鉴定变形菌属物种的16S和23S核糖核糖核酸（rRNA）或rRNA基因之间的ITS区域的新核酸序列，特别是变形杆菌，普通变形杆菌（Proteus vulgaris）和/或变形杆菌（Proteus penneri）。本发明还涉及使用衍生自ITS（内部转录间隔物）区域的所述新的核酸序列特异性检测和/或鉴定变形杆菌物种，特别是奇异变形杆菌，普通变形杆菌和/或变形杆菌的方法。它还涉及用于扩增样品中变形杆菌物种的所述间隔区的核酸引物。

3. 发明申请

WO0073499A3 NUCLEIC ACID PROBES AND METHODS FOR DETECTING CLINICALLY IMPORTANT FUNGAL PATHOGENS 审中-公开
标题翻译：用于检测临床重要真菌病原体的核酸探针和方法
公开(公告)号：WO0073499A3
公开(公告)日：2001-08-09
申请号：PCT/EP0004714
申请日：2000-05-24
申请人： INNOGENETICS NV , ENTPR IE TRD AS BIORESEARCH IE , SMITH TERRY , MAHER MAJELLA , MARTIN CARA , JANNES GEERT , ROSSAU RUDI , WEIDE MARJO V D
发明人： SMITH TERRY , MAHER MAJELLA , MARTIN CARA , JANNES GEERT , ROSSAU RUDI , VAN DER WEIDE MARJO
IPC分类号： C12N15/09 , C12Q1/68
CPC分类号： C12Q1/6895
摘要： The current invention relates to the field of detection and identification of clinically important fungi. More particularly, the present invention relates to species specific probes originating from the Internal Transcribed Spacer (ITS) region of rDNA for the detection of fungal species such as Candida albicans, Candida parapsilosis, Candida tropicalis, Candida kefyr, Candida krusei, Candida glabrata, Candida dubliniensis, Aspergillus flavus, Aspergillus versicolor, Aspergillus nidulans, Aspergillus fumigatus, Cryptococcus neoformans and Pneumocystis carinii in clinical samples, and methods using said probes.
摘要翻译：本发明涉及临床重要真菌的检测和鉴定领域。更具体地说，本发明涉及源自rDNA的内部转录间隔区（ITS）区域的物种特异性探针，用于检测真菌物种，例如白色念珠菌，披膜假丝酵母，热带假丝酵母，假丝酵母，假丝酵母，念珠菌玻璃假丝酵母（Candida dubliniensis），黄曲霉（Aspergillus flavus），白曲霉（Aspergillus versicolor），构巢曲霉（Aspergillus nodulans），烟曲霉（Aspergillus fumigatus），新型隐球酵母（Cryptococcus neoformans）和卡氏肺囊虫（Pneumocystis carinii），以及使用所述探针的方法。

4. 发明申请

WO1996000298A1 SIMULTANEOUS DETECTION, IDENTIFICATION AND DIFFERENTIATION OF EUBACTERIAL TAXA USING A HYBRIDIZATION ASSAY 审中-公开
标题翻译：使用杂交测定法同时检测，鉴定和鉴定非特异性TAXA
公开(公告)号：WO1996000298A1
公开(公告)日：1996-01-04
申请号：PCT/EP1995002452
申请日：1995-06-23
申请人： INNOGENETICS N.V. , JANNES, Geert , ROSSAU, Rudi , VAN HEUVERSWYN, Hugo
发明人： INNOGENETICS N.V.
IPC分类号： C12Q01/68
CPC分类号： C12Q1/689 , C12Q1/6888 , C12Q2600/16
摘要： The present invention relates to a method for detection and identification of at least one microorganism, or for the simultaneous detection of several microorganisms in a sample, comprising the steps of: (i) if need be releasing, isolating or concentrating the polynucleic acids present in the sample; (ii) if need be amplifying the 16S-23S rRNA spacer region, or a part of it, with at least one suitable primer pair; (iii) hybridizing the polynucleic acids of step (i) or (ii) with at least one and preferably more than one of the spacer probes as mentioned in table 1a or equivalents of thereof, under the appropriate hybridization and wash conditions, and/or with a taxon-specific probe derived from any of the spacer sequences as represented in figs. 1-103 under the same hybridization and wash conditions; (iv) detecting the hybrids formed in step (iii) with each of the probes used under appropriate hybridization and wash conditions; (v) identification of the microorganism(s) present in the sample from the differential hybridization signals obtained in step (iv).
摘要翻译：本发明涉及用于检测和鉴定至少一种微生物或同时检测样品中几种微生物的方法，包括以下步骤：（i）如果需要释放，分离或浓缩存在于例子; （ii）如果需要用至少一个合适的引物对扩增16S-23S rRNA间隔区或其一部分; （iii）在合适的杂交和洗涤条件下，将步骤（i）或（ii）的多核酸与表1a或其等同物中所提及的至少一种并优选多于一种的间隔探针杂交，和/或具有从图中所示的任何间隔序列衍生的分类群特异性探针。 1-103在相同的杂交和洗涤条件下; （iv）在适当的杂交和洗涤条件下使用每种探针检测在步骤（iii）中形成的杂交体; （v）从步骤（iv）中获得的差异杂交信号鉴定样品中存在的微生物。

5. 发明申请

WO2005075673A3 DETECTION, IDENTIFICATION AND DIFFERENTIATION OF PROTEUS SPECIES USING THE SPACER REGION 审中-公开
标题翻译：利用间隔区域对蛋白质种类的检测，鉴定和分化
公开(公告)号：WO2005075673A3
公开(公告)日：2005-12-01
申请号：PCT/EP2005050464
申请日：2005-02-03
申请人： INNOGENETICS NV , ROCHE GMBH , JANNES GEERT , MIJS WOUTER , HABERHAUSEN GERD , EMRICH THOMAS
发明人： JANNES GEERT , MIJS WOUTER , HABERHAUSEN GERD , EMRICH THOMAS
IPC分类号： C12Q1/68
CPC分类号： C12Q1/68 , C12Q1/689
摘要： The present invention relates to new nucleic acid sequences derived from the ITS region, between the 16S and 23S ribosomal ribonucleic acid (rRNA) or rRNA genes, to be used for the specific detection and/or identification of Proteus species, in particular of Proteus mirabilis, Proteus vulgaris and/or Proteus penneri, in a biological sample. The present invention relates also to a method for the specific detection and/or identification of Proteus species, in particular Proteus mirabilis, Proteus vulgaris and/or Proteus penneri, using said new nucleic acid sequences derived from the ITS (Internal Transcribed Spacer) region. It relates also to nucleic acid primers to be used for the amplification of said spacer region of Proteus species in a sample.
摘要翻译：本发明涉及用于特异性检测和/或鉴定变形杆菌物种，特别是奇异变形杆菌（Proteus mirabilis）的16S和23S核糖体核糖核酸（rRNA）或rRNA基因之间来自ITS区的新核酸序列，普通变形菌（Proteus vulgaris）和/或普通变形菌（Proteus penneri）。本发明还涉及使用衍生自ITS（内部转录间隔区）区域的所述新核酸序列特异性检测和/或鉴定变形杆菌种类，特别是奇异变形杆菌，普通变形杆菌和/或普通变形杆菌的方法。它还涉及用于扩增样品中变形菌物种的间隔区的核酸引物。

6. 发明申请

WO2004052606A3 DETECTION, IDENTIFICATION AND DIFFERENTIATION OF EUBACTERIAL TAXA USING A HYBRIDIZATION ASSAY 审中-公开
标题翻译：使用杂交测定法检测，鉴定和鉴别非特异性TAXA
公开(公告)号：WO2004052606A3
公开(公告)日：2004-07-29
申请号：PCT/EP0313907
申请日：2003-12-08
申请人： INNOGENETICS NV , ROCHE DIAGNOSTICS GMBH , CLAEYS SOFIE , JANNES GEERT , HABERHAUSEN GERD , EMRICH THOMAS , VERDOODT LIA
发明人： CLAEYS SOFIE , JANNES GEERT , HABERHAUSEN GERD , EMRICH THOMAS , VERDOODT LIA
IPC分类号： C12Q1/68
CPC分类号： C12Q1/689
摘要： The present invention relates to a method for the specific detection and/or identification of Staphylococcus species, in particular Staphylococcus aureus, using new nucleic acid sequences derived from the ITS (Internal Transcribed Spacer) region.The present invention relates also to said new nucleic acid sequences derived from the ITS region, between the 16S and 23S ribosomal ribonucleic acid (rRNA) or rRNA genes, to be used for the specific detection and/or identification of Staphylococcus species, in particular of S. aureus, in a biological sample.It relates also to nucleic acid primers to be used for the amplification of said spacer region of Staphylococcus species in a sample.
摘要翻译：本发明涉及使用来自ITS（内转录间隔区）的新核酸序列来特异性检测和/或鉴定葡萄球菌属物种，特别是金黄色葡萄球菌的方法。本发明还涉及所述新的核酸 16S和23S核糖核酸核糖核酸（rRNA）或rRNA基因之间的ITS区序列用于特异性检测和/或鉴定生物样品中金黄色葡萄球菌，特别是金黄色葡萄球菌的特异性检测和/或鉴定。还涉及用于在样品中扩增葡萄球菌物种的所述间隔区的核酸引物。

7. 发明申请

WO2004053148A1 METHOD FOR THE DETECTION OF PATHOGENIC GRAM POSITIVE BACTERIA FROM THE GENERA STAPHYLOCOCCUS, ENTEROCOCCUS AND STREPTOCOCCUS 审中-公开
标题翻译：用于从致病菌，肠杆菌和结肠癌中检测病原性阳性细菌的方法
公开(公告)号：WO2004053148A1
公开(公告)日：2004-06-24
申请号：PCT/EP2003/013545
申请日：2003-12-02
申请人： ROCHE DIAGNOSTICS GMBH , F. HOFFMANN-LA ROCHE AG , INNOGENETICS N.V. , HABERHAUSEN, Gerd , EMRICH, Thomas , ROSSAU, Rudi , JANNES, Geert , DE VOS, Daniel
发明人： HABERHAUSEN, Gerd , EMRICH, Thomas , ROSSAU, Rudi , JANNES, Geert , DE VOS, Daniel
IPC分类号： C12Q1/14
CPC分类号： C12Q1/689 , C12Q1/04 , C12Q1/14 , C12Q1/6851 , G01N2333/31 , G01N2333/315
摘要： The present invention is directed to a method for identification of a Gram positive pathogenic bacterium comprising (a) an amplification step with at least a first set of amplification primers capable of amplifying a preselected nucleic acid sequence region from a first predetermined sub-group of pathogenic Gram positive bacteria, (ab) a detection step with at least a first hybridization reagent capable of specifically detecting a preselected nucleic acid sequence region from said first predetermined sub-group of pathogenic Gram positive bacteria, said detection step comprising steps (aba) monitoring, whether hybridization has occurred at a preselected temperature, said occurrence of hybridization being indicative for at least the genus of the pathogenic organism present in the sample, and (abb) monitoring temperature dependence of hybridization, said temperature dependence being indicative for at least the species of said pathogenic Gram positive bacterium.
摘要翻译：本发明涉及用于鉴定革兰氏阳性致病菌的方法，其包括（a）扩增步骤，所述扩增步骤具有至少第一组扩增引物，所述第一组扩增引物能够从病原体的第一预定亚组扩增预选的核酸序列区革兰氏阳性菌，（ab）具有至少第一杂交试剂的检测步骤，所述第一杂交试剂能够特异性检测来自所述第一预定的病原性革兰氏阳性细菌亚组的预选核酸序列区，所述检测步骤包括步骤（aba）是否在预选温度下进行杂交，所述杂交的发生指示至少存在于样品中的病原生物的属，以及（abb）监测杂交的温度依赖性，所述温度依赖性至少指示所述致病性革兰氏阳性菌。

8. 发明申请

WO2006035062A1 DETECTION, IDENTIFICATION AND DIFFERENTIATION OF SERRATIA SPECIES USING THE SPACER REGION 审中-公开
标题翻译：使用间隔区域检测，鉴定和分类的SERRATIA物种
公开(公告)号：WO2006035062A1
公开(公告)日：2006-04-06
申请号：PCT/EP2005/054913
申请日：2005-09-29
申请人： INNOGENETICS N.V. , ROCHE DIAGNOSTICS GMBH , JANNES, Geert , MIJS, Wouter , EMRICH, Thomas , HABERHAUSEN, Gerd
发明人： JANNES, Geert , MIJS, Wouter , EMRICH, Thomas , HABERHAUSEN, Gerd
IPC分类号： C12Q1/68
CPC分类号： C12Q1/689 , Y10T436/143333
摘要： The present invention relates to new nucleic acid sequences derived from the ITS (Internal Transcribed Spacer) region, between the 16S and 23 S rRNA genes, to be used for the specific detection and/or identification of Serratia species, in particular of Serratia marcescens, Serratia ficaria and/or Serratia fonticola, in a biological sample. The present invention relates also to a method for the specific detection and/or identification of Serratia species, in particular Serratia marcescens, Serratia ficaria and/or Serratia fonticola, using said new nucleic acid sequences derived from the ITS region. It relates also to nucleic acid primers to be used for the amplification of said spacer region of Serratia species in a sample.
摘要翻译：本发明涉及来自16S和23S rRNA基因之间的ITS（内转录间隔区）的新核酸序列，用于特异性检测和/或鉴定沙雷氏菌属，尤其是粘质沙雷氏菌，在沙门氏菌属和/或沙雷氏菌中，在生物样品中。本发明还涉及使用来自ITS区域的所述新的核酸序列特异性检测和/或鉴定沙雷氏菌属物种，特别是沙雷氏菌属沙雷氏菌属，沙雷氏菌属和/或沙雷氏菌属的方法。它还涉及用于扩增样品中沙雷氏菌属物种的所述间隔区的核酸引物。

9. 发明申请

WO2004053156A1 DETECTION, IDENTIFICATION AND DIFFERENTIATION OF EUBACTERIAL TAXA USING A HYBRIDIZATION ASSAY 审中-公开
标题翻译：使用杂交测定法检测，鉴定和鉴定非特异性毒素
公开(公告)号：WO2004053156A1
公开(公告)日：2004-06-24
申请号：PCT/EP2003/013905
申请日：2003-12-08
申请人： INNOGENETICS N.V. , ROCHE DIAGNOSTICS GMBH , DE HENAU, Hilde , VAN CROMBRUGGEN, Joachim , JANNES, Geert , HABERHAUSEN, Gerd , EMRICH, Thomas
发明人： DE HENAU, Hilde , VAN CROMBRUGGEN, Joachim , JANNES, Geert , HABERHAUSEN, Gerd , EMRICH, Thomas
IPC分类号： C12Q1/68
CPC分类号： C12Q1/689
摘要： The present invention relates to a method for the specific detection and/or identification of Enterococcus species, in particular Enterococcus faecalis and/or Enterococcus faecium, using new nucleic acid sequences derived from the ITS (Internal Transcribed Spacer) region.The present invention relates also to said new nucleic acid sequences derived from the ITS region, between the 16S and 23S ribosomal ribonucleic acid (rRNA) or rRNA genes, to be used for the specific detection and/or identification of Enterococcus species, in particular of Enterococcus faecalis and/or Enterococcus faecium, in a biological sample.It relates also to nucleic acid primers to be used for the amplification of said spacer region of Enterococcus species in a sample.
摘要翻译：本发明涉及使用源自ITS（内转录间隔区）的新核酸序列来特异性检测和/或鉴定肠球菌属物种，特别是粪肠球菌和/或屎肠球菌的方法。本发明还涉及对来自ITS区域的所述新的核酸序列，16S和23S核糖核糖核酸（rRNA）或rRNA基因之间，用于特异性检测和/或鉴定肠球菌属物种，特别是粪肠球菌和/或生物样品中的屎肠球菌。还涉及用于在样品中扩增肠球菌属物种的所述间隔区的核酸引物。

10. 发明申请

WO9533851A3 METHOD FOR THE DETECTION OF THE ANTIBIOTIC RESISTANCE SPECTRUM OF MYCOBACTERIUM SPECIES 审中-公开
标题翻译：检测MYCOBACTERIUM物种抗生素抗性谱的方法
公开(公告)号：WO9533851A3
公开(公告)日：1996-02-08
申请号：PCT/EP9502230
申请日：1995-06-09
申请人： INNOGENETICS NV , BEENHOUWER HANS DE , PORTAELS FRANCOISE , MACHTELINCKX LIEVE , JANNES GEERT , ROSSAU RUDI
发明人： DE BEENHOUWER HANS , PORTAELS FRANCOISE , MACHTELINCKX LIEVE , JANNES GEERT , ROSSAU RUDI
IPC分类号： C12N15/09 , C07H21/04 , C12N15/00 , C12Q1/68 , C12R1/32
CPC分类号： C12Q1/689 , C12Q2600/156
摘要： Method for the detection of the antibiotic resistance spectrum of Mycobacterium species present in a sample, possibly coupled to the identification of the Micobacterium species involved, comprising the steps of: (i) if need be releasing, isolating or concentrating the polynucleic acids present in the sample; (ii) if need be amplifying the relevant part of the antibiotic resistance genes present in said sample with at least one suitable primer pair; (iii) hybridizing the polynucleic acids of step (i) or (ii) with at least one of the rpoB gene probes, as specified in table 2, under the appropriate hybridization and wash conditions; (iv) detecting the hybrids formed in step (iii); (v) inferring the Mycobacterium antibiotic resistance spectrum, and possibly the Mycobacterium species involved from the differential hybridization signal(s) obtained in step (iv).

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