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1. 发明申请

WO9923250A3 SPECIFIC AND SENSITIVE METHOD FOR DETECTING NUCLEIC ACIDS 审中-公开
标题翻译：特异性和敏感的核酸检测程序
公开(公告)号：WO9923250A3
公开(公告)日：1999-07-22
申请号：PCT/EP9806961
申请日：1998-11-03
申请人： ROCHE DIAGNOSTICS GMBH , KESSLER CHRISTOPH , HABERHAUSEN GERD , BARTL KNUT , ORUM HENRIK
发明人： KESSLER CHRISTOPH , HABERHAUSEN GERD , BARTL KNUT , ORUM HENRIK
IPC分类号： C12N15/09 , C12Q1/68
CPC分类号： C12Q1/6823 , C12Q1/6818 , C12Q1/686 , C12Q2561/101
摘要： The invention relates to a method for detecting a nucleic acid, comprising the following steps: the production of a number of enhancers of a fragment of said nucleic acid with a length of less than 100 nucleotides using two primers, one of said primers being able to bind to a first binding sequence (A) of a strand of the nucleic acid and the other primer being able to bind to a second binding sequence (C') which is essentially complementary to a sequence (C), said sequence (C) not overlapping with (A) and being positioned in the direction 3' from A, in the presence of a probe with a binding sequence (D) which is able to bind to the third sequence (B) positioned between the sequences (A) and (C) or the complement (B') thereof, said probe containing a reporter group and a quencher group, using a polymerase with 5' nuclease activity; and detection of the nucleic acid through the measurement of a signal which is determined by the release of the reporter group.
摘要翻译：本发明提供一种方法，用于检测的核酸，其包括制备多个所述核酸使用两种引物，其中一个与所述核酸的一条链的第一结合序列（A）结合一个小于100个核苷酸的长度的一部分的扩增子的步骤这些都可以可在其它结合第二结合序列（C“），该（C）是实质上以非重叠而与（a）中，位于3”选自（a）序列方向是互补的，在一个存在用的结合序列（d），该第三序列位于所述序列之间的（a）和（C）（B）或（B“）的互补探针可在其结合，用含报告基团和淬灭剂基团，所述探针，使用具有5'核酸酶活性的聚合酶并通过测量由于报道基团释放引起的信号来检测核酸。

2. 发明申请

WO8601209A3 NEW P-PHENYLENEDIAMINE PEPTIDES AND REACTANCE CONTAINING THEM FOR THE DETERMINATION OF PROTEASES OF THE BLOOD COAGULATION SYSTEM 审中-公开
标题翻译：新型对苯二甲胺类抗生素及其包含血液凝固系统蛋白质测定的反应
公开(公告)号：WO8601209A3
公开(公告)日：1986-03-27
申请号：PCT/EP8500385
申请日：1985-07-31
申请人： BOEHRINGER MANNHEIM GMBH , BARTL KNUT , BECKER UDO , ELTZ HERBERT VON DER , LILL HELMUT , BATZ HANS GEORG
发明人： BARTL KNUT , BECKER UDO , VON DER ELTZ HERBERT , LILL HELMUT , BATZ HANS-GEORG
IPC分类号： C07K1/113 , C07K5/06 , C07K5/08 , C07K5/083 , C07K5/087 , C07K5/10 , C07K5/103 , C07K7/00 , C07K14/675 , C07K14/81 , C12Q1/37 , C12Q1/56
CPC分类号： C12Q1/56 , C07K5/0806 , C07K5/0812 , C07K5/1005 , C07K14/675 , C12Q2337/10 , G01N2333/755
摘要： New compositions having the general formula (I), wherein A is the amino acid arginine or lysine, X is an N-terminal amino acid protection group or D-amino acid, Y is a single bound or a chain comprised of 1-3 amino acids, NR1R2 is a group in the o-position or p-position wherein R1 and R2 are independent from each other and represent hydrogen or alkyl having from 1 to 3 atoms of carbon and R3 is a hydrogen atom, a carboxyl group, a halogen atom or an alkyl group having from 1 to 3 atoms of carbon. Said compositions are useful as chromogenic substrates of proteases. They are obtained by reduction and optionally by alkylation of corresponding nitroanilides. A corresponding reactant to determine proteases of the blood coagulation system comprises a composition having the formula (I), an oxidation agent, an anyline-or phenol derivative which forms a dye stuff by oxidation with the phenylene diamine and a buffer substance.

3. 发明申请

WO9923249A3 SPECIFIC AND SENSITIVE METHOD FOR DETECTING NUCLEIC ACIDS 审中-公开
标题翻译：特异性和敏感性NUCLEIC检测方法
公开(公告)号：WO9923249A3
公开(公告)日：1999-09-10
申请号：PCT/EP9806952
申请日：1998-11-03
申请人： ROCHE DIAGNOSTICS GMBH , KESSLER CHRISTOPH , HABERHAUSEN GERD , BARTL KNUT , ORUM HENRIK
发明人： KESSLER CHRISTOPH , HABERHAUSEN GERD , BARTL KNUT , ORUM HENRIK
IPC分类号： C12N15/09 , C12Q1/68
CPC分类号： C12Q1/6823 , C12Q1/6818 , C12Q1/686 , C12Q2561/101
摘要： The invention relates to a method for detecting a nucleic acid comprising the production of a plurality of amplifications of a section of said nucleic acid with the assistance of two primers of which one can bond on a bonding sequence A of the nucleic acid and the other can bond on a bonding sequence C' which is complimentary to a sequence C. Sequence C does not overlap A and is situated in a 3' direction from A. The inventive method also includes bringing the amplifications in contact with a probe having a bonding sequence D which can bond on a sequence B, said sequence B being situated between sequences A and C, or the complement thereof. In addition, the invention relates to the detection of the construction of a hybrid out of the amplification and the probe, whereby the sequence situated between the bonding sequences A and C contains no nucleotides, said nucleotides not being linked to the bonding sequence D of the probe or to complement D' thereof.
摘要翻译：一种用于检测核酸，其包含准备多个所述核酸与两个引物，其中一个能够结合到核酸的结合序列A中的助剂和重叠于结合序列C“，它们不与其中另一个的一部分的扩增子的方法位于从序列C中的3“方向是相辅相成的，可以结合，与具有探针可结合的位置序列a和C序列B或补体之间它们的结合序列D，并检测该接触的扩增产物形成所述扩增产物和所述探针，其中位于所述结合序列a和C之间的序列不包含不属于该探针或其互补D”的D结合序列中的核苷酸的混合体。

4. 发明申请

WO9924606A3 SPECIFIC AND SENSITIVE NUCLEIC ACID DETECTION METHOD 审中-公开
标题翻译：特异性和敏感的核酸检测程序
公开(公告)号：WO9924606A3
公开(公告)日：1999-07-22
申请号：PCT/EP9806951
申请日：1998-11-03
申请人： ROCHE DIAGNOSTICS GMBH , KESSLER CHRISTOPH , HABERHAUSEN GERD , BARTL KNUT , ORUM HENRIK
发明人： KESSLER CHRISTOPH , HABERHAUSEN GERD , BARTL KNUT , ORUM HENRIK
IPC分类号： C12N15/09 , C12Q1/68
CPC分类号： C12Q1/6823 , C12Q1/6818 , C12Q1/686 , C12Q2561/101
摘要： The invention relates to a method for detecting a nucleic acid in which a plurality of amplificates of a section of the nucleic acid is produced with the aid of two primers. One primer can bond on a bonding sequence A of the nucleic acid, and the other can bond on a bonding sequence C' which is complimentary to a sequence C, does not overlap with A, and is placed in a 3'-direction of A. The amplificates are brought into contact with a probe having a bonding sequence D which can bond on a sequence B or the complement thereof, said sequence B and the complement thereof being placed between sequences A and C. The formation of a hybrid out of the amplificate and the probe is detected, whereby the sequence placed between bonding sequences A and C contains no nucleotides which do not belong to the bonding sequence D or complement D' thereof of the probe.
摘要翻译：一种用于检测核酸，其包含准备多个所述核酸与两个引物，其中一个能够结合到核酸的结合序列A中的助剂和重叠于结合序列C“，它们不与其中另一个的一部分的扩增子的方法位于从序列C中的3“方向是相辅相成的，可以结合，与具有探针可结合的位置序列a和C序列B或补体之间它们的结合序列d，并检测该接触的扩增产物形成扩增物和探针的杂交体，其中位于结合序列A和C之间的序列不含有不属于探针或其互补物D'的结合序列D的核苷酸。

5. 发明申请

WO1986001209A2 NEW p-PHENYLENEDIAMINE PEPTIDES AND REACTANCE CONTAINING THEM FOR THE DETERMINATION OF PROTEASES OF THE BLOOD COAGULATION SYSTEM 审中-公开
标题翻译：新型对苯二胺类肽及其含有血液凝固系统蛋白质测定的反应
公开(公告)号：WO1986001209A2
公开(公告)日：1986-02-27
申请号：PCT/EP1985000385
申请日：1985-07-31
申请人： BOEHRINGER MANNHEIM GMBH , BARTL, Knut , BECKER, Udo , VON DER ELTZ, Herbert , LILL, Helmut , BATZ, Hans-Georg
发明人： BOEHRINGER MANNHEIM GMBH
IPC分类号： C07K05/06
CPC分类号： C12Q1/56 , C07K5/0806 , C07K5/0812 , C07K5/1005 , C07K14/675 , C12Q2337/10 , G01N2333/755
摘要： New compositions having the general formula (I), wherein A is the amino acid arginine or lysine, X is an N-terminal amino acid protection group or D-amino acid, Y is a single bound or a chain comprised of 1-3 amino acids, NR1R2 is a group in the o-position or p-position wherein R1 and R2 are independent from each other and represent hydrogen or alkyl having from 1 to 3 atoms of carbon and R3 is a hydrogen atom, a carboxyl group, a halogen atom or an alkyl group having from 1 to 3 atoms of carbon. Said compositions are useful as chromogenic substrates of proteases. They are obtained by reduction and optionally by alkylation of corresponding nitroanilides. A corresponding reactant to determine proteases of the blood coagulation system comprises a composition having the formula (I), an oxidation agent, an anyline-or phenol derivative which forms a dye stuff by oxidation with the phenylene diamine and a buffer substance.

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