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1. 发明申请

WO2013004587A1 METHOD FOR SEPARATION OF MONOMERIC POLYPEPTIDES FROM AGGREGATED POLYPEPTIDES 审中-公开
标题翻译：从聚集多糖分离单体多聚体的方法
公开(公告)号：WO2013004587A1
公开(公告)日：2013-01-10
申请号：PCT/EP2012/062570
申请日：2012-06-28
申请人： F. HOFFMANN-LA ROCHE AG , BAEHNER, Monika , GROSSMANN, Adelbert , HEPBILDIKLER, Stefan
发明人： BAEHNER, Monika , GROSSMANN, Adelbert , HEPBILDIKLER, Stefan
IPC分类号： C07K1/18 , C07K1/20 , C07K14/54 , C07K19/00 , C07K1/36
CPC分类号： C07K1/165 , C07K1/18 , C07K1/20 , C07K1/36 , C07K14/5443 , C07K2319/30
摘要： Herein is reported a method for obtaining a polypeptide in monomeric form comprising the steps of a) providing a solution comprising the polypeptide in monomeric form and in aggregated form, wherein the ratio of monomeric to aggregated form is 4:1 or less as determined by size exclusion chromatography, b) performing a mixed-mode chromatography in bind-and-elute mode, or a hydrophobic interaction chromatography in flow-through mode, or a size-exclusion chromatography, and c) performing a weak cation exchange chromatography in bind-and-elute mode or flow-through mode, and thereby obtaining the polypeptide in monomeric form.
摘要翻译：本文报道了获得单体形式的多肽的方法，包括以下步骤：a）提供包含多肽的单体形式和聚集形式的溶液，其中单体与聚集形式的比例为4:1或更小，根据尺寸排阻层析，b）以结合 - 洗脱模式进行混合模式色谱法，或以流通模式或尺寸排阻色谱法进行疏水相互作用层析，以及c）在结合 - 和 - 洗脱模式中进行弱阳离子交换层析 - 微波模式或流通模式，从而获得单体形式的多肽。

2. 发明申请

WO2006072564A1 ANTIBODIES AGAINST IL-13 RECEPTOR ALPHA 1 AND USES THEREOF 审中-公开
标题翻译：抗IL-13受体ALPHA 1的抗体及其用途
公开(公告)号：WO2006072564A1
公开(公告)日：2006-07-13
申请号：PCT/EP2006/000005
申请日：2006-01-02
申请人： F.HOFFMANN-La ROCHE AG , ENDL, Josef , FUENTES, Maria , GRAUS, Yvo , GROSSMANN, Adelbert , NEUMANN, Sebastian , PARREN, Paul , REBERS, Frank , REGULA, Joerg , SCHUMACHER, Ralf , SEEBER, Stefan , STRACKE, Jan , STUBENRAUCH, Kay-Gunnar , VAN DE WINKEL, Jan , VAN VUGT, Martine , VEREECKEN-VERPLOEGEN, Sandra
发明人： ENDL, Josef , FUENTES, Maria , GRAUS, Yvo , GROSSMANN, Adelbert , NEUMANN, Sebastian , PARREN, Paul , REBERS, Frank , REGULA, Joerg , SCHUMACHER, Ralf , SEEBER, Stefan , STRACKE, Jan , STUBENRAUCH, Kay-Gunnar , VAN DE WINKEL, Jan , VAN VUGT, Martine , VEREECKEN-VERPLOEGEN, Sandra
IPC分类号： C07K16/28 , A61K39/395 , A61P11/06 , A61P37/08 , C12N15/13 , C12N15/79 , C12N15/70 , C12N5/10 , C12N5/20 , C12N1/21
CPC分类号： C07K16/2866 , C07K2317/21 , C07K2317/56 , C07K2317/76
摘要： An antibody binding to IL-13Rαl, inhibiting IL-13 bioactivity and comprising a variable heavy and a variable light chain, characterized in that the variable heavy chain amino acid sequence CDR3 of said antibody is selected from the group consisting of the heavy chain CDR3 sequences of SEQ ID NO: 1, 3, 5, 7 or 9 is useful in the treatment of asthma and allergic diseases.
摘要翻译：与IL-13Ral结合的抗体，其抑制IL-13生物活性并包含可变重链和可变轻链，其特征在于所述抗体的可变重链氨基酸序列CDR3选自重链CDR3序列的SEQ ID NO：1,3,5,7或9可用于治疗哮喘和过敏性疾病。

3. 发明申请

WO1991010910A2 PROCESS FOR ENZYMATICALLY TREATING SUBSTRATES 审中-公开
标题翻译：用于酶处理基材的方法
公开(公告)号：WO1991010910A2
公开(公告)日：1991-07-25
申请号：PCT/EP1991000086
申请日：1991-01-18
申请人： BOEHRINGER MANNHEIM GMBH , RUDOLPH, Rainer , KOPETZKI, Erhard , FISCHER, Stephan , GROSSMANN, Adelbert , HÖLL-NEUGEBAUER, Bärbel
发明人： BOEHRINGER MANNHEIM GMBH
IPC分类号： G01N33/543
CPC分类号： C07K14/005 , C07K2319/00 , C07K2319/40 , C07K2319/735 , C12N15/62 , C12N2740/16122 , C12N2740/16222 , G01N33/535 , G01N33/543 , G01N33/56988
摘要： In order to treat a substrate with enzymes, the substrate to be treated is brought into contact with a biocatalyzer obtained by combining a gene that codes for a biologically active substance and a fragment of DNA that codes for a binding peptide capable of interacting with a supporting base, to produce a fusion protein. The fusion protein is inserted in an appropriate vector, transformed in an appropriate organism, the organism is cultivated, its cells are dissolved and the lysate containing the fusion protein is brought into contact with a supporting base capable of binding with the binding peptide. The fusion protein is bound to the supporting base by intermolecular interaction, and the enzymatically treated substrate is thus finally obtained.
摘要翻译：为了用酶处理底物，将待处理的底物与通过组合编码生物活性物质的基因和编码能够与支持物相互作用的结合肽的DNA片段获得的生物催化剂接触碱，产生融合蛋白。将融合蛋白插入合适的载体中，转化到合适的生物体中，培养生物体，将其细胞溶解，使含有融合蛋白的裂解物与能与结合肽结合的支持碱接触。通过分子间相互作用将融合蛋白结合到支持碱基上，从而最终获得酶处理的底物。

4. 发明申请

WO2013144091A1 MICRO FLOW FILTRATION SYSTEM AND FLOW FILTRATION METHOD 审中-公开
标题翻译：微流量过滤系统和流量过滤方法
公开(公告)号：WO2013144091A1
公开(公告)日：2013-10-03
申请号：PCT/EP2013/056298
申请日：2013-03-25
申请人： ROCHE DIAGNOSTICS GMBH , F. HOFFMANN-LA ROCHE AG , Oranth, Norbert , LOSLEBEN, Nadine , LUTZ, Sascha , GROSSMANN, Adelbert
发明人： Oranth, Norbert , LOSLEBEN, Nadine , LUTZ, Sascha , GROSSMANN, Adelbert
IPC分类号： B01D61/18 , B01D61/20
CPC分类号： B01D61/22 , B01D61/147 , B01D61/18 , B01D61/20 , B01D2311/14 , B01D2313/10 , B01D2313/14 , B01D2313/243 , B01D2313/28 , B01D2313/50 , B01D2315/10 , B01D2315/12
摘要： A micro flow filtration system comprises a fluid circuitry (3) and a first reservoir (1) outside the circuitry (3) suitable for containing a fluid. The fluid circuitry (3) comprises a tangential flow filtration module (10) capable of separating the fluid sample into a retentate stream and a permeate stream upon passage of the fluid sample into the tangential flow filtration module (10) through an inlet feed (18). The fluid circuitry (3) further comprises a second reservoir (2) integrated in the fluid circuitry (3), a pump (5) for creating and driving a fluid flow, optionally at least one pressure sensor (6, 7 or 8) for acquiring and detecting data about the fluid sample, optionally a pressure regulator (9) for regulating the flow in the fluid circuitry (3) and a plurality of conduits (22) forming the fluid circuitry (3) together with the second reservoir (2), the TFF-module (10), the pump (5), the pressure sensor (6, 7 or 8) (if present) and the pressure regulator (9) (if present). The volume of the first reservoir (1) outside the circuitry (3) is significantly larger than the volume of the second reservoir (2). The first reservoir (1) outside the fluid circuitry (3) is connected to the circuitry (3) via a connection conduit (31) such that the fluid flows unidirectionally into the circuitry (3) until the first reservoir (1) is empty so that a continuous fluid flow from the first reservoir (1) to the circuitry (3) is established.
摘要翻译：微流过滤系统包括流体回路（3）和适于容纳流体的电路（3）外部的第一储存器（1）。流体回路（3）包括切向流过滤模块（10），该切向流过滤模块（10）能够通过入口进料（18）将流体样品分离成切向流过滤模块（10），该流动过滤模块能够将流体样品分离成滞留物流和渗透物流）。流体回路（3）还包括集成在流体回路（3）中的第二贮存器（2），用于创建和驱动流体流的泵（5），可选地至少一个压力传感器（6,7或8）获取和检测关于流体样本的数据，可选地用于调节流体回路（3）中的流动的压力调节器（9）和与第二储存器（2）一起形成流体回路（3）的多个导管（22），TFF模块（10），泵（5），压力传感器（6,7或8）（如果存在）和压力调节器（9）（如果存在）。电路（3）外部的第一储存器（1）的体积显着大于第二储存器（2）的体积。流体回路（3）外部的第一储存器（1）经由连接导管（31）连接到电路（3），使得流体单向流入电路（3），直到第一储存器（1）为空建立从第一储存器（1）到电路（3）的连续流体流动。

5. 发明申请

WO2013144089A1 MICRO FLOW FILTRATION SYSTEM AND INTEGRATED MICROFLUIDIC ELEMENT 审中-公开
标题翻译：微流过滤系统和集成微流体元件
公开(公告)号：WO2013144089A1
公开(公告)日：2013-10-03
申请号：PCT/EP2013/056294
申请日：2013-03-25
申请人： ROCHE DIAGNOSTICS GMBH , F. HOFFMANN-LA ROCHE AG , ORANTH, Norbert , LOSLEBEN, Nadine , LUTZ, Sascha , GROSSMANN, Adelbert
发明人： ORANTH, Norbert , LOSLEBEN, Nadine , LUTZ, Sascha , GROSSMANN, Adelbert
IPC分类号： G01N1/40 , B01D63/00 , B01D63/08 , B01L3/00
CPC分类号： B01D63/005 , B01D61/147 , B01D61/22 , B01D63/087 , B01D63/088 , B01D2311/14 , B01D2311/246 , B01D2311/25 , B01D2313/90 , B01D2315/10 , B01L3/502715 , B01L3/502753 , B01L2200/146 , B01L2300/0681 , B01L2300/0816 , B01L2300/088 , B01L2400/0478 , B01L2400/0487 , G01N2001/4088
摘要： A micro fluid filtration system (100) preferably for increasing the concentration of components contained in a fluid sample has a fluid circuitry (1). The fluid circuitry (1) comprises the following elements: A tangential flow filtration element (7) capable for separating the fluid sample into a retentate stream and a permeate stream upon passage of the fluid, an element for pumping (3) for creating and driving a fluid flow through the fluid circuitry (1) and at least one element for obtaining information about the properties of the fluid sample within the circuitry. The circuitry further comprises a plurality of conduits (24) connecting the elements of the fluid circuitry (1) through which a fluid stream of the fluid sample is conducted. The circuitry (1) has a minimal working volume of at most 5 ml, which is the minimal fluid volume retained in the elements and the conduits (24) of the circuitry (1) such that the fluid can be recirculated in the circuitry (1) without pumping air through the circuitry (1). An integrated microfluidic element (20) of the circuitry (1) contains the functionality of at least two elements of the group of elements of the circuitry (1).
摘要翻译：微流体过滤系统（100）优选地用于增加包含在流体样品中的组分的浓度具有流体回路（1）。流体回路（1）包括以下元件：切向流过滤元件（7），其能够在流体通过时将流体样品分离成滞留物流和渗透物流，用于泵送的元件（3）用于产生和驱动通过流体电路（1）的流体流和至少一个元件，用于获得关于电路内的流体样品的属性的信息。电路还包括连接流体回路（1）的元件的多个导管（24），流体样品的流体流通过该导管传导。电路（1）具有至多5ml的最小工作体积，其是保留在电路（1）的元件和导管（24）中的最小流体体积，使得流体可以在电路（1）中再循环）而不通过电路（1）抽空。电路（1）的集成微流体元件（20）包含电路（1）的元件组中的至少两个元件的功能。

6. 发明申请

WO2013127816A1 ON-COLUMN ENZYMATIC CLEAVAGE 审中-公开
公开(公告)号：WO2013127816A1
公开(公告)日：2013-09-06
申请号：PCT/EP2013/053872
申请日：2013-02-27
申请人： F. HOFFMANN-LA ROCHE AG , FALKENSTEIN, Roberto , GROSSMANN, Adelbert , HESSE, Friederike
发明人： FALKENSTEIN, Roberto , GROSSMANN, Adelbert , HESSE, Friederike
IPC分类号： C07K1/22 , C07K17/06 , C07K14/775 , C07K14/47
CPC分类号： C12P21/06 , B01D15/3828 , C07K1/22 , C07K14/4726 , C07K14/65 , C07K14/775 , C07K2319/00 , C07K2319/21 , C07K2319/735
摘要： Herein is reported a method for obtaining a polypeptide by an immobilized metal ion affinity chromatography from a pro-polypeptide that comprise at its N- or C-terminus an metal ion affinity chromatography tag and a protease cleavage site comprising the step of recovering the polypeptide from the immobilized metal ion affinity chromatography column by incubating the bound pro-polypeptide with a protease, whereby the immobilized metal ion affinity chromatography material has been washed at least once with an urea solution.
摘要翻译：本文报道了一种通过固定化金属离子亲和层析从前体多肽获得多肽的方法，所述前体多肽在其N-或C-末端包含金属离子亲和层析标签和蛋白酶切割位点，所述蛋白酶切割位点包括从通过将结合的原多肽与蛋白酶一起孵育来固定金属离子亲和层析柱，由此固定的金属离子亲和色谱材料已用尿素溶液洗涤至少一次。

7. 发明申请

WO2013127752A1 METHOD FOR REDUCTION OF 1->3 READING FRAME SHIFTS 审中-公开
标题翻译：减少1-> 3读取帧的方法
公开(公告)号：WO2013127752A1
公开(公告)日：2013-09-06
申请号：PCT/EP2013/053753
申请日：2013-02-26
申请人： F. HOFFMANN-LA ROCHE AG , GROSSMANN, Adelbert , HESSE, Friederike , KOPETZKI, Erhard , LAU, Wilma , SCHANTZ, Christian
发明人： GROSSMANN, Adelbert , HESSE, Friederike , KOPETZKI, Erhard , LAU, Wilma , SCHANTZ, Christian
IPC分类号： C07K14/775 , C12N15/67
CPC分类号： C07K14/775 , C07K14/4726 , C07K2319/00 , C12N15/67
摘要： Herein is reported a method for the recombinant production of a polypeptide, which comprises the tripeptide QKK, characterized in that the method comprises the step of recovering the polypeptide from the cells or the cultivation medium of a cultivation of a cell comprising a nucleic acid encoding the polypeptide and thereby producing the polypeptide, whereby the tripeptide QKK comprised in the polypeptide is encoded by the oligonucleotide cag aaa aaa or the oligonucleotide caa aag aaa.
摘要翻译：本文报道了重组产生多肽的方法，其包含三肽QKK，其特征在于该方法包括从细胞或培养基中培养多肽的步骤，该细胞或培养基包含编码多肽，从而产生多肽，由此多肽中包含的三肽QKK由寡核苷酸cag aaa aaa或寡核苷酸caa aag aaa编码。

8. 发明申请

WO2007110339A1 ANTI-IGF-1R HUMAN MONOCLONAL ANTIBODY FORMULATION 审中-公开
标题翻译：抗IGF-1R人单核抗体配方
公开(公告)号：WO2007110339A1
公开(公告)日：2007-10-04
申请号：PCT/EP2007/052569
申请日：2007-03-19
申请人： F. HOFFMANN-LA ROCHE AG , GROSSMANN, Adelbert , MAHLER, Hanns-Christian , PAPPENBERGER, Astrid , STAUCH, Oliver Boris , STRACKE, Jan Olaf
发明人： GROSSMANN, Adelbert , MAHLER, Hanns-Christian , PAPPENBERGER, Astrid , STAUCH, Oliver Boris , STRACKE, Jan Olaf
IPC分类号： A61K39/395 , C07K16/28 , A61P35/00
CPC分类号： A61K39/39591 , C07K16/2863 , C07K2317/21
摘要： The present invention relates to an anti-IGF-1R human monoclonal antibody formulation, a process for the preparation and uses thereof.
摘要翻译：本发明涉及抗IGF-1R人单克隆抗体制剂，其制备方法和用途。

9. 发明申请

WO2005113592A2 INTERFERON-ALPHA POLYPEPTIDES AND CONJUGATES 审中-公开
标题翻译：干扰素多肽和结合蛋白
公开(公告)号：WO2005113592A2
公开(公告)日：2005-12-01
申请号：PCT/US2005017471
申请日：2005-05-18
申请人： MAXYGEN INC , HOFFMANN LA ROCHE , PATTEN PHILLIP A , VISWANATHAN SRIDHAR , NISSEN TORBEN LAUESGAARD , VOGT ANNE , KROPSHOFER HARALD , SCHUMACHER RALF , FISCHER STEPHAN , SEEBER STEFAN , GROSSMANN ADELBERT , HESSE FRIEDERIKE , SCHAUBMAR ANDREAS , FALKENSTEIN ROBERTO , KOLL HANS , DEMBOWSKI MARKUS
发明人： PATTEN PHILLIP A , VISWANATHAN SRIDHAR , NISSEN TORBEN LAUESGAARD , VOGT ANNE , KROPSHOFER HARALD , SCHUMACHER RALF , FISCHER STEPHAN , SEEBER STEFAN , GROSSMANN ADELBERT , HESSE FRIEDERIKE , SCHAUBMAR ANDREAS , FALKENSTEIN ROBERTO , KOLL HANS , DEMBOWSKI MARKUS
IPC分类号： A61K38/21 , A61K47/48 , C07H21/04 , C07K14/555 , C07K14/56 , C12P21/02 , C12Q1/68
CPC分类号： A61K38/212 , A61K47/60 , C07K14/56
摘要： The present invention provides interferon-alpha polypeptides and conjugates, and nucleic acids encoding the polypeptides. The invention also includes compositions comprising these polypeptides, conjugates, and nucleic acids; cells containing or expressing the polypeptides, conjugates, and nucleic acids; methods of making the polypeptides, conjugates, and nucleic acids; and methods of using the polypeptides, conjugates, and nucleic acids.
摘要翻译：本发明提供了干扰素-α多肽和缀合物，以及编码多肽的核酸。本发明还包括包含这些多肽，缀合物和核酸的组合物; 含有或表达多肽，缀合物和核酸的细胞; 制备多肽，缀合物和核酸的方法; 以及使用多肽，缀合物和核酸的方法。

10. 发明申请

WO2002062773A1 TRICYCLIC LACTAM AND SULTAM DERIVATIVES AND THEIR USE AS HISTONE DEACETYLASE INHIBITORS 审中-公开
标题翻译： TRICYCLIC LACTAM和SULTAM衍生物及其用作HISTONE脱乙酰壳多糖酶抑制剂
公开(公告)号：WO2002062773A1
公开(公告)日：2002-08-15
申请号：PCT/EP2002/000705
申请日：2002-01-24
申请人： F. HOFFMANN-LA ROCHE AG , GEORGES, Guy , GROSSMANN, Adelbert , MUNDIGL, Olaf , VON DER SAAL, Wolfgang , SATTELKAU, Tim
发明人： GEORGES, Guy , GROSSMANN, Adelbert , MUNDIGL, Olaf , VON DER SAAL, Wolfgang , SATTELKAU, Tim
IPC分类号： C07D275/06
CPC分类号： C07D275/06 , C07D209/92
摘要： A compound of formula (I) wherein A and B are optionally substituted cyclohexenyl or phenyl groups, X is carbonyl or sulfonyl, and Y is 5-7 atoms-linker, is useful as histone deacetylase inhibitors, particularly for inhibiting cell proliferation.
摘要翻译：式（I）的化合物，其中A和B为任选取代的环己烯基或苯基，X为羰基或磺酰基，Y为5-7个原子的连接体，可用作组蛋白脱乙酰酶抑制剂，尤其用于抑制细胞增殖。

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