会员体验
专利管家(专利管理)
工作空间(专利管理)
风险监控(情报监控)
数据分析(专利分析)
侵权分析(诉讼无效)
联系我们
交流群
官方交流:
QQ群: 891211   
微信请扫码    >>>
现在联系顾问~
热词
    • 6. 发明申请
    • METHODS FOR MODULATING CELLULAR AND ORGANISMAL PHENOTYPES
    • 调节细胞和有机基因的方法
    • WO0173000A3
    • 2002-06-27
    • PCT/US0109203
    • 2001-03-23
    • MAXYGEN INCSTEMMER WILLEM P CMINSHULL JEREMYKEENAN ROBERT J
    • STEMMER WILLEM P CMINSHULL JEREMYKEENAN ROBERT J
    • C12N15/10C12N15/113C12N15/66C12N15/11C12N15/63C12Q1/68
    • C12N15/1079C07K2319/00C12N15/1027C12N15/1034C12N15/113C12N15/66C12N2310/111
    • Methods for identifying and controlling the genetic and metabolic pathways underlying complex phenotypes are provided. Conjoint polynucleotide segments that contribute to or disrupt elements of a multigenic phenotype are produced and expressed in cells of interest. Conjoint polynucleotide segments are recombined and/or mutated to give rise to libraries of recombinant concatamers which are expressed in cells of interest. Libraries of conjoint polynucleotide segments and recombinant concatamers are expressed episomally or integrated into the DNA of organelles or chromosomes. Cells are screened or selected to identify members of the population of cells exhibiting a desired phenotype. Libraries and vectors comprising conjoint polynucleotide segments and recombinant concatamers, as well as cells expressing such libraries and vectors or their components are provided. Kits containing conjoint polynucleotide segments, recombinant concatamers, vectors including such polynucleotides, and cells including such polynucleotides and vectors are provided.
    • 提供了鉴定和控制复杂表型下遗传和代谢途径的方法。 有助于或破坏多基因表型的元件的连接多核苷酸片段在感兴趣的细胞中产生并表达。 连接的多核苷酸片段被重组和/或突变以产生在感兴趣的细胞中表达的重组连接体的文库。 联合多核苷酸片段和重组连接体的文库以附加方式表达或整合到细胞器或染色体的DNA中。 筛选或选择细胞以鉴定呈现所需表型的细胞群体的成员。 提供了包含组合多核苷酸片段和重组连接体的文库和载体,以及表达这些文库和载体或其组分的细胞。 提供了含有联合多核苷酸片段,重组连接体,包含此类多核苷酸的载体和包含此类多核苷酸和载体的细胞的试剂盒。