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    • 5. 发明申请
    • TAGGED BINDING REAGENTS AND METHODS FOR DETECTING TARGET ANALYTES
    • 用于检测目标分析的标签绑定试剂和方法
    • WO1988006732A1
    • 1988-09-07
    • PCT/US1987003342
    • 1987-12-15
    • ALLIED CORPORATION
    • ALLIED CORPORATIONREGINA, Francis, JeffreyDIAMOND, Steven, ElliotBOYLE, William, Johnston, Jr.MASILAMANI, Divakaran
    • G01N33/531
    • G01N33/532
    • The present invention relates to methods of assay of target analyte binding pair members, and to the reagents useful for carrying out such assays. More specifically, the invention relates to ligand binding or nucleic acid assays using a reagent that comprises a signal moiety, and a moiety complementary to or homologous with all or a portion of the target analyte binding members. Under the appropriate conditions, the signal moiety produces a detectable moiety which can be transferred to a gaseous phase for detection. To serve this purpose, the signal moiety is a moiety capable of reacting with an effective second agent to produce a detectable moiety as a product of that reaction or is a moiety capable of being cleaved from the tagged binding pair member itself, volatilized and detected. The methods and reagents herein described are particularly applicable in the analysis of physiological and biological samples derived from human as well as from animal, plant, and microorganismic sources. In addition, the methods and reagents may be employed in connection with environmental analyses, specifically, in assays for suspect chemical or biological constituents or contaminents in food, water, air, soil samples, and the like.
    • 本发明涉及测定靶分析物结合对成员的方法,以及用于进行此类测定的试剂。 更具体地,本发明涉及使用包含信号部分的试剂和与全部或部分靶分析物结合成员互补或同源的部分的配体结合或核酸测定。 在适当的条件下,信号部分产生可检测的部分,其可转移到气相进行检测。 为了达到这一目的,信号部分是能够与有效的第二试剂反应以产生作为该反应的产物的可检测部分的部分,或者是能够从标记的结合对成员本身切割的部分,其挥发和检测。 本文所述的方法和试剂特别适用于衍生自人以及动物,植物和微震源的生理和生物样品的分析。 此外,方法和试剂可用于环境分析,特别是在食品,水,空气,土壤样品等中可疑的化学或生物成分或污染物的测定中。
    • 7. 发明申请
    • MASTER CYLINDER WITH FAST FILL VALVING MECHANISM
    • 具有快速封闭机构的主缸
    • WO1988005738A1
    • 1988-08-11
    • PCT/US1987002932
    • 1987-11-10
    • ALLIED CORPORATION
    • ALLIED CORPORATIONGAISER, Robert, Frank
    • B60T11/224
    • B60T11/224
    • The master cylinder (10) comprises a housing (12) having a longitudinal bore (14), a housing end (16) enclosed by a bearing member (18) which retains a sleeve member (20) within the bore (14), a reservoir (70) mounted on the housing (12) and a pair of pistons (30, 40) received slidably within the bore (14), one piston (30) extending through an end (24) of the sleeve member (20). The one piston has an end (32) which provides seating for a fast fill valving mechanism (110). The fast fill valving mechanism (110) comprises a piston support guide (120) supporting a fast fill seal (150) and seal retainer (160) between the support guide (120) and the surface (23) of the sleeve member (20). The seal retainer (160) engages one end (142) of a spring (140), the other spring end (143) located at the end (32) of the piston. Displacement of the one piston (30) within the bore (14) creates an increasing pressure which will, at a predetermined higher pressure, cause the spring (140) to collapse or bend and permit axial displacement of the seal (150) and seal retainer (160) away from sealing engagement with a portion (123) of the piston support guide (120), and thereby permit fast fill fluid pressure within the primary chamber (88) to communicate with an auxiliary chamber (180).