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81. 发明申请

WO2008083259A1 SYSTEMS AND METHODS FOR DETECTING NUCLEIC ACIDS 审中-公开
标题翻译：用于检测核酸的系统和方法
公开(公告)号：WO2008083259A1
公开(公告)日：2008-07-10
申请号：PCT/US2007/089007
申请日：2007-12-28
申请人： APPLERA CORPORATION , AIVAZACHVILI, Vissarion , SCABOO, Kristian , SPIER, Eugene
发明人： AIVAZACHVILI, Vissarion , SCABOO, Kristian , SPIER, Eugene
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6823 , C12Q1/6825 , C12Q1/6827 , C12Q2531/113 , C12Q2521/307 , C12Q2525/161 , C12Q2525/301 , C12Q2565/607 , C12Q2565/519 , C12Q2521/301 , C12Q2561/109
摘要： A method and kit for detecting a target nucleic acid in a sample is described. The sample to be analyzed may include a primer which hybridizes to at least a portion of the target nucleic acid, a probe having a first region which hybridizes to at least a portion of the target nucleic acid and a second region having a detectable label, a polymerase which extends the hybridized primer and an enzyme comprising exonuclease activity that can cleave the hybridized hybridization probe to thereby generate a labeled probe fragment. At least one portion of the hybridization probe hybridizes to another portion of the hybridization probe to thereby form a folded structure. The method can involve melting the sample, reducing the temperature of the sample to allow primer and probe to each hybridize to at least a portion of single stranded target nucleic acid in the sample, elongating the primer and releasing the labeled probe fragment. The sample can be contacted with a solid support comprising surface bound capture probes which hybridize to the labeled probe fragments. The label can then be detected.
摘要翻译：描述了用于检测样品中靶核酸的方法和试剂盒。要分析的样品可以包括与靶核酸的至少一部分杂交的引物，具有与靶核酸的至少一部分杂交的第一区的探针和具有可检测标记的第二区，扩增杂交引物的聚合酶和包含外切核酸酶活性的酶，其可以切割杂交的杂交探针，从而产生标记的探针片段。至少一部分杂交探针与杂交探针的另一部分杂交，从而形成折叠结构。该方法可以包括熔化样品，降低样品的温度以使引物和探针各自与样品中的至少一部分单链靶核酸杂交，延长引物并释放标记的探针片段。样品可以与包含与标记的探针片段杂交的表面结合捕获探针的固体支持物接触。然后可以检测标签。

82. 发明申请

WO2008072209A2 A METHOD AND A MICRODEVICE FOR THE IDENTIFICATION AND/OR QUANTIFICATION OF AN ANALYTE IN A BIOLOGICAL SAMPLE 审中-公开
标题翻译：用于生物样品中分析物的鉴定和/或定量的方法和微型装置
公开(公告)号：WO2008072209A2
公开(公告)日：2008-06-19
申请号：PCT/IB2007055112
申请日：2007-12-14
申请人： CONSIGLIO NAZIONALE RICERCHE , FOND ISTITUTO ITALIANO DI TECN , POMPA PIER PAOLO , SABELLA STEFANIA , RINALDI ROSARIA , CINGOLANI ROBERTO , CALABI FRANCO
发明人： POMPA PIER PAOLO , SABELLA STEFANIA , RINALDI ROSARIA , CINGOLANI ROBERTO , CALABI FRANCO
IPC分类号： G01N33/542 , C12Q1/68
CPC分类号： C12Q1/6818 , B82Y5/00 , C12Q1/6825 , C12Q1/6834 , G01N33/542 , C12Q2565/101 , C12Q2565/519 , C12Q2565/601
摘要： A method and device, based on a film of a luminescent substance, such as colloidal semiconductor nanocrystals dispersed in a polymer matrix, for conducting quantitative and real-time analyses of PCR processes or of biomolecular interactions in genomics and/or proteomics. The optical detection system is based on FRET processes between the luminescent substance (which acts as the donor in the FRET process) and a suitable fluorophore (which acts as the acceptor species) with which the DNA or other biomolecule is marked. The device is essentially composed of a reaction microchamber with a wall formed by a thin film made of polymer material, in which the nanocrystals are uniformly dispersed, or made of a photoluminescent or electroluminescent polymer. Molecular probes are chemically immobilized on the surface of the polymer film for the specific recognition of the analyte which is to be determined in real time. The film of nanocrystals is excited by radiation at low wavelength (for example, UV/blue), and the radiation in the spectral emission window characteristic of the fluorescent marker of the biomolecule is detected. The specific photophysical characteristics of FRET processes make it possible to monitor in a selective way, in real time and in quantitative mode, the biomolecular interactions, which take place in the close proximity of the surface of the film (typically at distances of
摘要翻译：基于分散在聚合物基质中的发光物质如胶体半导体纳米晶体的膜的方法和装置，用于进行PCR过程的定量和实时分析或基因组学和/或蛋白质组学中的生物分子相互作用。光学检测系统基于发光物质（其作为FRET过程中的供体）与标记DNA或其他生物分子的合适的荧光团（其作为受体物质）之间的FRET过程。该装置主要由具有由聚合物材料制成的薄膜形成的壁的反应微室组成，其中纳米晶体均匀分散，或由光致发光或电致发光聚合物制成。分子探针化学固定在聚合物膜的表面上，用于特异性识别实时测定的分析物。纳米晶体的膜由低波长（例如UV /蓝色）的辐射激发，并检测生物分子的荧光标记特征的光谱发射窗口的辐射。 FRET过程的特定光物理特性使得可以以选择性的方式在实时和定量模式下监测在膜表面附近发生的生物分子相互作用（通常在<10纳米的距离处）），从而几乎完全减少由背景信号和溶液中的游离生物分子引起的可能的干扰（其没有与相应的识别位点相互作用）。该装置的特征还使得能够在不同的生物分子（复用）上并行进行同时分析。

83. 发明申请

WO2008062479A2 A HIGH SENSITIVITY ASSAY FOR MOLECULAR TYPING OF BIOLOGICAL SAMPLE, PROBES AND A KIT THEREOF 审中-公开
标题翻译：高灵敏度分析生物样本，探针及其试剂盒的分子类型
公开(公告)号：WO2008062479A2
公开(公告)日：2008-05-29
申请号：PCT/IN2007000543
申请日：2007-11-19
申请人： JAWAHARLAL NEHRU CT FOR ADVANC , MICROTEST INNOVATIONS PVT LTD , RANGA UDAYKUMAR , NARAYANA CHANDRABHAS , NARAYANA JAYASURYAN
发明人： RANGA UDAYKUMAR , NARAYANA CHANDRABHAS , NARAYANA JAYASURYAN
CPC分类号： C12Q1/6827 , C12Q1/703 , C12Q2565/632 , C12Q2565/519 , C12Q2537/125
摘要： The present invention relates to a high sensitivity assay for molecular typing of a biological sample using surface-enhanced Raman scattering (SERS) including resonance scattering (SERRS); capture probes for capturing nucleic acid; a detector probe to detect captured nucleic acid; a kit for molecular typing of biological sample using surface-enhanced Raman scattering (SERS) including resonance scattering (SERRS); and lastly a method of manufacturing said kit.
摘要翻译：本发明涉及使用包括共振散射（SERRS）的表面增强拉曼散射（SERS）对生物样品的分子分型进行高灵敏度测定; 用于捕获核酸的捕获探针; 用于检测捕获的核酸的检测器探针; 使用包括共振散射（SERRS）的表面增强拉曼散射（SERS）对生物样品进行分子分型的试剂盒; 最后是制造所述试剂盒的方法。

84. 发明申请

WO2008054556A2 METHODS AND COMPOSITIONS FOR DETECTION OF A TARGET NUCLEIC ACID SEQUENCE 审中-公开
标题翻译：用于检测目标核酸序列的方法和组合物
公开(公告)号：WO2008054556A2
公开(公告)日：2008-05-08
申请号：PCT/US2007014588
申请日：2007-06-22
申请人： STRATAGENE CALIFORNIA , SORGE JOSEPH A
发明人： SORGE JOSEPH A
IPC分类号： C12Q1/68 , G01N33/48 , G01N33/50 , G06F19/00
CPC分类号： G01N33/5308 , C12Q1/48 , C12Q1/6823 , C12Q2521/119 , C12Q2521/301 , C12Q2531/113 , C12Q2521/307 , C12Q2525/301 , C12Q2565/519 , C12Q2561/109 , C12Q2525/161
摘要： The invention relates to compositions and methods for generating a signal indicative of the presence of a target nucleic acid in a sample, where the compositions and methods include an RNA polymerase, a FEN nuclease, and a probe.
摘要翻译：本发明涉及用于产生指示样品中目标核酸存在的信号的组合物和方法，其中组合物和方法包括RNA聚合酶，FEN核酸酶和探针。

85. 发明申请

WO2008027548A2 MICROARRAY-BASED GLOBAL CHROMATIN STRUCTURE MAPPING 审中-公开
标题翻译：基于MICROARRAY的全球色谱结构绘图
公开(公告)号：WO2008027548A2
公开(公告)日：2008-03-06
申请号：PCT/US2007/019196
申请日：2007-08-31
申请人： DANA-FARBER CANCER INSTITUTE, INC. , FISHER, David, E. , LIU, Xiaole, S. , SONG, Jun, S. , OZSOLAK, Fatih
发明人： FISHER, David, E. , LIU, Xiaole, S. , SONG, Jun, S. , OZSOLAK, Fatih
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6804 , C12Q2565/519 , C12Q2522/101
摘要： Microarray-based methods for identifying positions of protein-DNA complexes on genomic DNA, including in particular human genomic DNA, are provided. The methods can be used to locate positioned nucleosomes, pre-initiation complexes, and other protein-DNA complexes on a global level, thereby permitting gene expression profiling based on microarray-based analysis of genomic DNA. Methods are provided to characterize and compare various cell types, including cancer cells. Also provided are methods for screening effects of test agents and pharmaceutical agents, including histone deacetylase inhibitors. Also provided are methods for identifying transcription start sites, transcriptionally active promoters, transcription factor binding sites, and transcription factors involved in the regulation of gene expression, including for microRNAs.
摘要翻译：提供了用于鉴定蛋白质-DNA复合物在基因组DNA上的位置（包括特别是人类基因组DNA）的基于微阵列的方法。该方法可用于在全球范围内定位定位的核小体，起始前复合物和其他蛋白质-DNA复合物，从而基于基于基因组DNA分析的基因表达谱。提供方法来表征和比较各种细胞类型，包括癌细胞。还提供了用于筛选试验剂和药剂（包括组蛋白脱乙酰酶抑制剂）的作用的方法。还提供了用于鉴定转录起始位点，转录活性启动子，转录因子结合位点和参与调节基因表达的转录因子（包括用于微小RNA）的方法。

86. 发明申请

WO2008016988A1 METHODS OF NONSPECIFIC TARGET CAPTURE OF NUCLEIC ACIDS 审中-公开
标题翻译：核酸非靶向捕获方法
公开(公告)号：WO2008016988A1
公开(公告)日：2008-02-07
申请号：PCT/US2007074990
申请日：2007-08-01
申请人： GEN PROBE INC , BECKER MICHAEL M , MAJLESSI MEHRDAD R
发明人： BECKER MICHAEL M , MAJLESSI MEHRDAD R
IPC分类号： C12Q1/68 , C07H21/04
CPC分类号： C12N15/1006 , C12N15/11 , C12N2310/18 , C12N2310/31 , C12N2310/321 , C12N2310/351 , C12N2330/30 , C12Q1/6806 , C12Q1/6834 , C12Q1/70 , C12Q2565/519 , C12Q2565/518
摘要： Methods for capturing a target nucleic acid from a sample by using a capture probe that binds nonspecifically to the target nucleic acid and binds specifically to an immobilized probe via a specific binding pair that has one member on the capture probe and one member on the immobilized probe are disclosed. Compositions that include a capture probe that binds nonspecifically to a target nucleic acid and specifically to an immobilized probe via binding of members of a specific binding pair in a solution phase of a reaction mixture are disclosed.
摘要翻译：通过使用捕获探针非特异性结合靶核酸并通过在捕获探针上具有一个成员的特异性结合对与固定化探针上的一个成员特异性结合固定的探针来从样品捕获靶核酸的方法被披露。公开了包含捕获探针的组合物，其通过结合反应混合物的溶液相中的特异性结合对的成员非特异性地结合靶核酸而特异性结合固定的探针。

87. 发明申请

WO2007065028A2 SOLID PHASE RFLP-BASED SNP DETECTION 审中-公开
标题翻译：基于RFLP的固定相SNP检测
公开(公告)号：WO2007065028A2
公开(公告)日：2007-06-07
申请号：PCT/US2006/046354
申请日：2006-12-04
申请人： GENE CHECK, INC.
发明人： SCHELL, Allan, C.P. , DANIELL, Erica, L.
IPC分类号： C12Q1/68 , C12P19/34 , C07H21/04
CPC分类号： C12Q1/683 , C12Q2565/518 , C12Q2565/102 , C12Q2565/519
摘要： The invention is directed to a method for detection of single nucleotide polymorphisms utilizing a solid phase RFLP-based method of detection. The invention further relates to a method of detecting a single nucleotide polymorphism (SNP) comprising providing a sample test DNA molecule containing an SNP site of interest; detectably labeling the DNA molecule at or near each of its ends with different labels and containing the SNP site of interest; providing at least two different immobilization oligonucleotides capable of immobilization to a solid support; digesting the test DNA molecule with a restriction endonuclease, whose cut site contains the SNP site in at least one allele of the SNP site; separating strands of the test DNA molecule; annealing the strands to the immobilization oligonucleotides; and detecting the labels of DNA strands annealed to the immobilization oligonucleotide.
摘要翻译：本发明涉及利用基于固相RFLP的检测方法检测单核苷酸多态性的方法。本发明还涉及检测单核苷酸多态性（SNP）的方法，其包括提供含有目的SNP位点的样品测试DNA分子; 可检测地用不同的标签将DNA分子标记在每个末端附近，并含有目的SNP位点; 提供能够固定在固体支持物上的至少两种不同的固定化寡核苷酸; 用限制性内切核酸酶消化测试DNA分子，其切割位点在SNP位点的至少一个等位基因中含有SNP位点; 分离测试DNA分子的链; 将链退火至固定化寡核苷酸; 并检测退火固定寡核苷酸的DNA链的标记。

88. 发明申请

WO2006034349A3 DNA FINGERPRINTING USING A BRANCH MIGRATION ASSAY 审中-公开
标题翻译：使用分支移动测定DNA指纹
公开(公告)号：WO2006034349A3
公开(公告)日：2006-08-24
申请号：PCT/US2005033838
申请日：2005-09-21
申请人： OR UNIVERSITY THE BOARD OF TRU , POURMAND NADER , DAVIS RONALD W , WANG SHAN X
发明人： POURMAND NADER , DAVIS RONALD W , WANG SHAN X
IPC分类号： C12Q1/68 , C07H21/02 , C07H21/04
CPC分类号： C12Q1/68 , C12Q1/6837 , C12Q2565/519 , C12Q2537/1373 , C12Q2525/204
摘要： A method of determining the length of a polynucleotide target is provided. With this method, a target is first hybridized to an array of first probes having different, determined lengths, resulting in the formation of duplexes between the polynucleotide target and the first probes. These duplexes have a single stranded section of target if the target is longer than the first probe it is in a duplex with. Next, a second probe having a determined length is hybridized to these duplexes. If the length of the target is greater than the length of the first probe it is displaced during this hybridization step by the process of branch migration. In contrast, if the length of the target is less than or equal to the length of the first probe, it is not displaced. Thus, the length of the polynucleotide target can be determined.
摘要翻译：提供了确定多核苷酸靶标长度的方法。利用该方法，首先将靶与具有不同的确定长度的第一探针阵列杂交，导致在多核苷酸靶和第一探针之间形成双链体。如果目标比第一个探针更长，那么这些双链体具有目标单链段。接下来，具有确定长度的第二探针与这些双链体杂交。如果靶的长度大于第一探针的长度，则在该杂交步骤期间通过分支迁移的过程被置换。相反，如果目标的长度小于或等于第一探针的长度，则其不被移位。因此，可以确定多核苷酸靶的长度。

89. 发明申请

WO2006076793A1 DNA SEQUENCE RECOGNITION 审中-公开
标题翻译： DNA序列识别
公开(公告)号：WO2006076793A1
公开(公告)日：2006-07-27
申请号：PCT/CA2006/000051
申请日：2006-01-18
申请人： GRADE BIOSENSE INC. , DUDELZAK, Alexander, E. , GINOVKER, Andrey , RAKITIN, Andrei
发明人： DUDELZAK, Alexander, E. , GINOVKER, Andrey , RAKITIN, Andrei
IPC分类号： C12Q1/68 , G01N27/49
CPC分类号： B82Y15/00 , B82Y30/00 , B82Y40/00 , C12Q1/6825 , C12Q2565/519 , C12Q2563/161 , C12Q2565/607
摘要： To detect the presence of a match of a target DNA base sequence with a probe DNA base sequence, a single strand of the probe DNA base sequence is prepared. One end of the single strand probe DNA base sequence is linked to an electrode and the other end to a nano entity capable of exchanging charge with the DNA base sequence. The single strand of the target DNA base sequence is brought into contact with the single strand of the probe DNA base sequence, and the change in the physical properties of the probe DNA base sequence upon hybridization detected.
摘要翻译：为了检测目标DNA碱基序列与探针DNA碱基序列的匹配的存在，制备探针DNA碱基序列的单链。单链探针DNA碱基序列的一端连接到电极，另一端连接到能够与DNA碱基序列交换电荷的纳米实体。使靶DNA碱基序列的单链与探针DNA碱基序列的单链接触，并且检测到杂交时探针DNA碱基序列的物理性质的变化。

90. 发明申请

WO2006075497A1 プローブユニット、ヌクレオチド領域の同定装置、及びヌクレオチド領域の同定方法审中-公开
标题翻译：探测单元，识别核酸区域的装置和识别核酸区域的方法
公开(公告)号：WO2006075497A1
公开(公告)日：2006-07-20
申请号：PCT/JP2005/023539
申请日：2005-12-21
申请人：国立大学法人京都大学 , 岡本晃充 , 亀井琢 , 齋藤烈
发明人：岡本晃充 , 亀井琢 , 齋藤烈
IPC分类号： G01N27/416 , C12M1/34 , C12N15/09 , C12Q1/68 , G01N27/327 , G01N33/53 , G01N33/543
CPC分类号： G01N33/5438 , C12Q1/6825 , C12Q2565/519 , C12Q2563/113
摘要：　標的核酸中の目的ヌクレオチド領域を同定するためのプローブユニットであって、電極と、電極に結合した、標的核酸を認識するプローブと、プローブに結合したホール移動誘起剤とを備え、プローブのホール移動誘起剤結合位置と電極結合末端との間に目的ヌクレオチド領域と対合するヌクレオチド領域が位置しているプローブユニット。このプローブユニットと標的核酸とをハイブリダイズさせた前後のエネルギー誘起ホール移動による電気化学的信号の大きさを比較することにより、標的核酸中の目的ヌクレオチド領域の状態を同定できる。
摘要翻译：用于鉴定提供有电极的目标核酸中的靶核苷酸区域的探针单元，与电极结合的能够识别目标核酸的探针和结合到探针的空穴转移诱导剂，其中与靶核苷酸区域配对的核苷酸区域位于探针的空穴转移诱导剂结合位点和电极的结合端之间。通过比较由于能量诱导的空穴转移而使杂交后的探针单元与靶核酸的电化学信号强度与杂交后的电化学信号强度相比较，可以鉴定目标核酸中的靶核苷酸区域的状态。

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