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    • 78. 发明申请
    • HYDROLASES, NUCLEIC ACIDS ENCODING THEM AND METHODS FOR IMPROVING PAPER STRENGTH
    • 水解物,核酸编码方法及提高纸张强度的方法
    • WO2006096834A2
    • 2006-09-14
    • PCT/US2006/008555
    • 2006-03-08
    • DIVERSA CORPORATIONKEROVUO, Janne, S.MCCANN, RyanWEINER, DavidSOLBAK, Arne, I., Jr.
    • KEROVUO, Janne, S.MCCANN, RyanWEINER, DavidSOLBAK, Arne, I., Jr.
    • C12N9/42
    • C12P7/6454C11C1/045C11C3/08C11C3/10C12N9/16C12N9/20C12P7/62C12P7/6418C12P7/6427Y10T442/20
    • The invention provides hydrolases, polynucleotides encoding them, and methods of making and using these polynucleotides and polypeptides. In one aspect, the invention is directed to polypeptides, e.g., enzymes, having a hydrolase activity, e.g., an esterase, acylase, lipase, phospholipase (e.g., phospholipase A, B, C and D activity, patatin activity, lipid acyl hydrolase (LAH) activity) or protease activity, including thermostable and thermotolerant hydrolase activity, and polynucleotides encoding these enzymes, and making and using these polynucleotides and polypeptides. The hydrolase activities of the polypeptides and peptides of the invention include esterase activity, lipase activity (hydrolysis of lipids), acidolysis reactions (to replace an esterified fatty acid with a free fatty acid), transesterification reactions (exchange of fatty acids between triglycerides), ester synthesis, ester interchange reactions, phospholipase activity and protease activity (hydrolysis of peptide bonds). In another aspect, the invention provides methods for hydrolyzing steryl esters and triglycerides (e.g., in a paper pulp), into sterols, glycerol and free fatty acids, using enzyme(s) of the invention. The invention provides enzymes and methods for decreasing the amount of lipophilic extracts ("pitch") in a pulp- comprising composition. The polypeptides of the invention can be used in a variety of pharmaceutical, agricultural and industrial contexts, including the manufacture of cosmetics and nutraceuticals.
    • 本发明提供水解酶,编码它们的多核苷酸,以及制备和使用这些多核苷酸和多肽的方法。 一方面,本发明涉及具有水解酶活性的多肽,例如酯酶,酰基转移酶,脂肪酶,磷脂酶(例如,磷脂酶A,B,C和D活性,patatin活性,脂质酰基水解酶( LAH)活性)或蛋白酶活性,包括耐热和耐热水解酶活性,以及​​编码这些酶的多核苷酸,以及制备和使用这些多核苷酸和多肽。 本发明的多肽和肽的水解酶活性包括酯酶活性,脂肪酶活性(脂质水解),酸解反应(用游离脂肪酸代替酯化脂肪酸),酯交换反应(甘油三酸酯之间的脂肪酸交换), 酯合成,酯交换反应,磷脂酶活性和蛋白酶活性(肽键的水解)。 另一方面,本发明提供了使用本发明的酶将甾醇酯和甘油三酯(例如在纸浆中)水解成甾醇,甘油和游离脂肪酸的方法。 本发明提供用于减少含纸浆组合物中的亲脂性提取物(“沥青”)量的酶和方法。 本发明的多肽可用于各种药物,农业和工业环境中,包括制造化妆品和营养品。