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61. 发明申请

WO03042233A2 METHODS FOR MONITORING POLYPEPTIDE PRODUCTION AND PURIFICATION USING SURFACE ENHANCED LASER DESORPTION/IONIZATION MASS SPECTROMETRY 审中-公开
标题翻译：使用表面增强激光吸收/离子质谱法监测多肽生产和纯化的方法
公开(公告)号：WO03042233A2
公开(公告)日：2003-05-22
申请号：PCT/US0235971
申请日：2002-11-08
申请人： CIPHERGEN BIOSYSTEMS INC
发明人： BOSCHETTI EGISTO , BRADBURY LISA , DAVIES HUW , LOMAS LEE , PHAM THANG T , THULASIRAMAN VANITHA , YIP TAI-TUNG
IPC分类号： G01N27/62 , C07K1/16 , C07K1/18 , C07K1/20 , C07K1/22 , C07K14/47 , C12P21/02 , G01N27/64 , G01N30/72 , G01N30/84 , G01N30/88 , G01N33/68 , C07K
CPC分类号： G01N33/6842 , C07K1/16 , C07K1/18 , C07K1/20 , C07K1/22 , G01N33/6803 , G01N2030/8411 , H01J49/00 , H01J49/0418 , G01N30/7233
摘要： The invention provides methods of monitoring the production of a target polypeptide by generating surface enhanced laser desorption/ionization mass spectral profiles of samples taken form multiple cell culture batches or of samples taken at different times from a given batch. The invention additionally provides methods for monitoring the purification of a target polypeptide from a mixture by generating surface enhanced laser desorption/ionization mass spectral profiles of samples taken at various times during a purification process. In addition, the invention relates to methods of identifying conditions that can be used in increasing the scale of a given purification process.
摘要翻译：本发明提供了通过产生从多个细胞培养物批次或在从给定批次的不同时间获取的样品获取的样品的表面增强的激光解吸/电离质谱图来监测目标多肽的产生的方法。本发明另外提供了通过产生在纯化过程中的不同时间采集的样品的表面增强的激光解吸/电离质谱图来监测来自混合物的目标多肽的纯化的方法。此外，本发明涉及鉴定可用于增加给定纯化方法的规模的条件的方法。

62. 发明申请

WO0232537A3 IMPROVED METHOD OF EXPANDED BED CHROMATOGRAPHY 审中-公开
标题翻译：改进的扩展床层析方法
公开(公告)号：WO0232537A3
公开(公告)日：2002-07-11
申请号：PCT/US0127736
申请日：2001-09-28
申请人： LILLY CO ELI , PROUTY WALTER FRANCIS JUNIOR
发明人： PROUTY WALTER FRANCIS JUNIOR
IPC分类号： B01D15/02 , B01D15/18 , B01D15/36 , C07K1/18 , C07K14/575 , C07K14/605 , G01N30/58 , C07K1/16
CPC分类号： B01D15/1807 , B01D15/362 , B01D15/363 , C07K1/18 , C07K14/57563 , C07K14/605 , G01N30/58
摘要： The present invention is a method of separating a desired component (e.g., a biomolecule such as a recombinant protein) from a mixture such as an unclarified feedstock. In a first step, the mixture is solubilized in an aqueous urea solution to form a sample solution. In a second step, the sample solution is passed upwards through a bed of resin, thereby binding the desired component to the resin and separating the desired component from the mixture. The sample solution comprises a sufficiently high concentration of urea to reduce fouling of resin when the sample solution is passed through the resin.
摘要翻译：本发明是从混合物例如未澄清的原料中分离出所需组分（例如，生物分子如重组蛋白质）的方法。在第一步中，将混合物溶解在尿素水溶液中以形成样品溶液。在第二步中，样品溶液向上通过树脂床，由此将所需组分与树脂结合并从混合物中分离出所需组分。样品溶液包含足够高浓度的尿素，以在样品溶液通过树脂时减少树脂的结垢。

63. 发明申请

WO0158924A3 NOVEL RESIN MATERIALS AND THEIR USE FOR RECOVERING PROTEINS OR PEPTIDES 审中-公开
标题翻译：新型树脂材料及其用于恢复蛋白质或肽的用途
公开(公告)号：WO0158924A3
公开(公告)日：2002-05-02
申请号：PCT/DK0100086
申请日：2001-02-08
申请人： UPFRONT CHROMATOGRAPHY AS , LIHME ALLAN OTTO FOG , HANSEN MARIE BENDIX , BISGAARD FRANTZEN HANS , RAHBEK NIELSEN HENRIK
发明人： LIHME ALLAN OTTO FOG , HANSEN MARIE BENDIX , BISGAARD-FRANTZEN HANS , RAHBEK-NIELSEN HENRIK
IPC分类号： B01D15/08 , B01J20/32 , C07K1/18 , C07K1/20 , C12N9/64 , C07K1/00 , B01J20/00
CPC分类号： B01J20/3253 , B01D15/08 , B01J20/3204 , B01J20/321 , B01J20/3212 , B01J20/3217 , B01J20/3242 , B01J20/3248 , B01J20/3255 , B01J20/3285 , C07K1/18 , C07K1/20 , C12N9/6478 , C12N9/6481 , C12N9/6483 , C12Y304/23004
摘要： Processes for recovering a target protein having milk clotting activity including chymosin, and other target proteins or peptides from an aqueous medium containing such a protein using a resin material comprising a solid support matrix and, covalently bound to the matrix, a target protein binding ligand selected from benzylamine or a derivative thereof; an alkylamine, an alkenylamine, an alkynylamine, an alkoxyamine and an amine of mono- or bicyclic aromatic or heteroaromatic moieties, any of which is optionally substituted with at least one group other than an acidic group. The resin material may conveniently be in a packed or a fluidised or expanded bed.
摘要翻译：使用包含固体支持基质的树脂材料和与基质共价结合所选择的靶蛋白结合配体，从含有这种蛋白质的水性培养基中回收具有乳凝活性的凝乳活性的靶蛋白（包括凝乳酶）和其它靶蛋白或肽的方法来自苄胺或其衍生物; 烷基胺，链烯基胺，炔基胺，烷氧基胺和单环或双环芳族或杂芳族部分的胺，其中任何一个任选被除酸性基团以外的至少一个基团取代。树脂材料可以方便地在填充或流化或膨胀的床中。

64. 发明申请

WO01072769A3 METHODS FOR PURIFYING HIGHLY ANIONIC PROTEINS 审中-公开
标题翻译：净化高亲和性蛋白质的方法
公开(公告)号：WO01072769A3
公开(公告)日：2002-02-07
申请号：PCT/US2001/009815
申请日：2001-03-27
IPC分类号： C07K14/00 , C07K1/14 , C07K1/18 , C07K1/20 , C07K14/705 , C07K19/00
CPC分类号： C07K1/20 , C07K1/18 , C07K1/36 , C07K14/70596 , C07K2319/00 , C07K2319/30
摘要： The present invention provides a process for isolating and purifying highly anionic target proteins, for example, sulfated proteins. Sulfated proteins have five (5) or more, sulfations. In a preferred embodiment, the sulfated protein has six (6) sulfations, e.g., six sulfations on N-terminal tyrosine residues, as embodied in PSGL-1 (P-selectin glycoprotein ligand).
摘要翻译：本发明提供了用于分离和纯化高度阴离子的靶蛋白，例如硫酸化蛋白质的方法。硫酸化蛋白质有五（5）或更多硫酸盐。在优选的实施方案中，如在PSGL-1（P-选择蛋白糖蛋白配体）中体现的，硫酸化蛋白质具有六（6）个硫酸盐，例如在N末端酪氨酸残基上的六个硫酸盐。

65. 发明申请

WO0048703B1 PURIFICATION OF BIOLOGICAL SUBSTANCES 审中-公开
标题翻译：净化生物物质
公开(公告)号：WO0048703B1
公开(公告)日：2000-12-07
申请号：PCT/US0040035
申请日：2000-02-22
申请人： KOPF HENRY
发明人： KOPF HENRY
IPC分类号： G01N33/548 , B01D15/08 , B01D15/26 , B01D61/02 , B01D61/14 , B01D61/24 , B01J20/281 , C07K1/18 , C07K1/22 , C07K1/34 , C07K1/36 , C12M1/12 , C12M3/06 , C12N7/02 , C12N7/04 , C12N7/06 , C12N9/00 , G01N30/88
CPC分类号： C07K1/22 , B01D15/361 , B01D15/3809 , C07K1/18 , C07K1/34 , C07K1/36
摘要： A process and apparatus (20) for purifying one or more target substances from a source liquid, employing one or more cross-flow filter elements (27), and one or more types of chromatography resins, in combination, to provide purification with advantageous yield, product purity, and cost- and time-efficiency.
摘要翻译：一种用于从源液中净化一种或多种目标物质的方法和装置（20），其使用一种或多种交叉流过滤元件（27）和一种或多种类型的色谱树脂组合以提供有利的纯度，产品纯度，成本和时间效率。

66. 发明申请

WO00015655A1 PROCESS FOR PRODUCING PEPTIDES FROM BIOLOGICAL FLUIDS AND PEPTIDES OBTAINABLE BY SAID PROCESS 审中-公开
标题翻译：从生物流体生产肽的方法和通过合适的方法获得的肽
公开(公告)号：WO00015655A1
公开(公告)日：2000-03-23
申请号：PCT/EP1999/007002
申请日：1999-09-15
IPC分类号： C12P21/06 , A61K38/00 , A61K38/01 , A61K38/10 , A61P31/00 , A61P31/12 , A61P35/00 , C07K1/12 , C07K1/16 , C07K1/18 , C07K1/20 , C07K1/22 , C07K7/06 , C07K7/08 , C07K14/315 , C07K14/47 , C07K14/79
CPC分类号： C07K7/08 , A61K38/01 , C07K1/12 , C07K1/18 , C07K1/22 , C07K14/315 , C07K14/4723 , C07K14/4732 , C07K14/79
摘要： A process is provided for the production of at least one peptide of interest from a biological fluid comprising one or more proteins which contain the amino acid sequence of said peptide, wherein said fluid is contacted with a chromatographic medium to adsorb said protein including the peptide domain, the adsorbed material is hydrolysed to produce said peptide, the medium is washed to remove unbound material, the peptide is desorbed from the chromatographic medium and, optionally, further purified. Preferably, a chromatographic membrane is used and the hydrolysis is preferably carried out enzymatically. Also claimed are peptides, preferably with a biological activity, which are obtainable by the present process.
摘要翻译：提供了用于从包含一种或多种含有所述肽的氨基酸序列的蛋白质的生物流体生产至少一种目的肽的方法，其中所述流体与色谱介质接触以吸附所述蛋白质，包括肽结构域，吸附的材料被水解以产生所述肽，洗涤介质以除去未结合的物质，肽从色谱介质解吸，并且任选进一步纯化。优选地，使用色谱膜，并且优选在酶促下进行水解。还要求的是优选具有生物活性的肽，其可通过本方法获得。

67. 发明申请

WO99047574A1 QUATERNARY AMMONIUM POLYMER, ITS PREPARATION, ITS USE FOR SEPARATING BIOMOLECULES 审中-公开
标题翻译：季铵聚合物及其制备方法，用于分离生物分子
公开(公告)号：WO99047574A1
公开(公告)日：1999-09-23
申请号：PCT/IB1999/000455
申请日：1999-03-18
IPC分类号： B01D15/42 , C07K1/18 , C08F26/04 , C08F126/04
CPC分类号： B01D15/422 , C07K1/18 , C08F126/04
摘要： A linear water-soluble quaternary ammonium polymer having a homopolymeric chain of repeating unit of general formula (I): where each of R1 and R2 independently represents a member selected from the group consisting of linear or branched alkyl, hydroxyalkyl, alkoxyalkyl groups having from 1 to 6 carbon atoms, X represents an anion and n is an integer comprised between 30 and 220 has been prepared. Furthermore, the polymer has a molar mass distribution of less than or equal to 1.5. The polymer is used as a displacer in a displacement mode of chromatography.
摘要翻译：一种具有通式（I）的重复单元的均聚链的直链水溶性季铵聚合物：其中R 1和R 2各自独立地表示选自直链或支链烷基，羟基烷基，烷氧基烷基的成员，其具有1 至6个碳原子，X'表示阴离子，n是包含在30和220之间的整数。此外，聚合物的摩尔质量分布小于或等于1.5。该聚合物用于置换色谱法中的置换器。

68. 发明申请

WO99031120A1 NOVEL TGF-BETA PROTEIN PURIFICATION METHODS 审中-公开
标题翻译：新型TGF-β蛋白纯化方法
公开(公告)号：WO99031120A1
公开(公告)日：1999-06-24
申请号：PCT/US1998/026208
申请日：1998-12-10
IPC分类号： C07K1/18 , C07K14/495
CPC分类号： C07K1/18 , C07K14/495
摘要： Method of purifying TGF- beta superfamily proteins, including osteogenic proteins, such as bone morphogenetic proteins (BMPs), are disclosed.
摘要翻译：公开了纯化TGF-β超家族蛋白质的方法，包括成骨蛋白质，例如骨形态发生蛋白（BMP）。

69. 发明申请

WO1998000441A1 A METHOD FOR CHROMATOGRAPHIC REMOVAL OF PRIONS 审中-公开
标题翻译：色谱去除方法
公开(公告)号：WO1998000441A1
公开(公告)日：1998-01-08
申请号：PCT/US1997011149
申请日：1997-06-26
申请人： BIOPURE CORPORATION
发明人： BIOPURE CORPORATION , GAWRYL, Maria, S. , HOUTCHENS, Robert, A. , LIGHT, William, R.
IPC分类号： C07K14/17
CPC分类号： C07K14/805 , C07K1/18
摘要： A method for removing a prion from a solution comprising the prion and at least one additional biomolecule, comprising directing the solution through an anion-exchange chromatography column under conditions that cause a gradient elution, whereby the prion is separated from at least one of the biomolecules, thereby causing said biomolecule to be collected in an eluate fraction that is distinct from an eluate fraction that includes the prion. In one embodiment, the gradient is a pH gradient, for example, a step gradient. The prion can be a causal agent for a spongiform encephalopathy, such as Creutzfeldt-Jakob disease, Gerstmann-Straussler-Scheinken syndrome, scrapie, or bovine spongiform encephalopathy.
摘要翻译：一种从包含朊病毒和至少一种另外的生物分子的溶液中除去朊病毒的方法，包括在引起梯度洗脱的条件下将溶液引导通过阴离子交换层析柱，由此将朊病毒与至少一种生物分子分离，从而使所述生物分子被收集在不同于包括朊病毒的洗脱液部分的洗脱液组分中。在一个实施方案中，梯度是pH梯度，例如梯度梯度。朊病毒可以是海绵状脑病的致病因子，例如Creutzfeldt-Jakob病，Gerstmann-Straussler-Scheinken综合征，瘙痒病或牛海绵状脑病。

70. 发明申请

WO1997011090A1 INHIBITION OF TUMOR GROWTH 审中-公开
标题翻译：抑制肿瘤生长
公开(公告)号：WO1997011090A1
公开(公告)日：1997-03-27
申请号：PCT/US1996014512
申请日：1996-09-17
申请人： WEISZ, Paul, B.
IPC分类号： C07K01/22
CPC分类号： A61K31/335 , A61K31/395 , A61K31/57 , A61K31/70 , A61K31/724 , A61K31/737 , A61K35/32 , A61K47/6951 , A61L15/28 , A61L26/0023 , A61L27/20 , A61L27/34 , A61L27/54 , A61L33/0005 , A61L2300/222 , A61L2300/406 , A61L2300/408 , A61L2300/416 , A61L2300/802 , B82Y5/00 , C07K1/18 , C07K2/00 , C07K14/50 , C07K14/503 , C08B37/0012 , A61K2300/00 , C08L5/16
摘要： Anti-angiogenic protein composition can be prepared simply and efficiently by contacting cartilage particles containing the protein together with an electrolyte solution and an oligosaccharide bearing a minimum number of anionic substituents per sugar unit, to extract the anti-angiogenic protein from the cartilage particles and to form a complex of the oligosaccharide and the protein. The resultant anti-angiogenic complex can be used for therapeutic treatment of various angiogenic and related diseases.
摘要翻译：通过使包含蛋白质的软骨颗粒与电解质溶液和每个糖单元具有最少数量的阴离子取代基的寡糖接触，可以简单有效地制备抗血管生成蛋白质组合物，以从软骨颗粒提取抗血管生成蛋白质，形成寡糖和蛋白质的复合物。所得抗血管生成复合物可用于治疗各种血管生成和相关疾病。

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