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1. 发明申请

WO2015154048A1 DEVICES AND METHODS FOR PURIFICATION, DETECTION AND USE OF BIOLOGICAL CELLS 审中-公开
标题翻译：用于净化，检测和使用生物细胞的装置和方法
公开(公告)号：WO2015154048A1
公开(公告)日：2015-10-08
申请号：PCT/US2015/024374
申请日：2015-04-03
申请人： GJERDE, Douglas T.
发明人： GJERDE, Douglas T.
IPC分类号： B01D15/08
CPC分类号： C07K1/16 , B01D15/166 , B01D15/3804 , B01D15/422 , B01D15/428 , C07K1/22
摘要： This invention relates to devices and methods for purifying, detecting and using biological cells. A variety of cell types including viable tumor, stem, immune and sperm cells can be purified from a complex biological sample using a column, including a pipette tip column. Methods of the invention can aid research, diagnosis and treatment of cancer. Purified viable cells can be detected on the column or eluted from the column and detected. Cells on a column can be used as a stationary phase for liquid chromatography. Cells may be removed, recovered and analyzed.
摘要翻译：本发明涉及用于纯化，检测和使用生物细胞的装置和方法。可以使用柱（包括移液管尖端柱）从复杂的生物样品中纯化包括活的肿瘤，茎，免疫和精子细胞的多种细胞类型。本发明的方法可以帮助研究，诊断和治疗癌症。可以在柱上检测纯化的活细胞或从柱上洗脱并检测。柱上的细胞可用作液相色谱的固定相。可以除去，回收和分析细胞。

2. 发明申请

WO2011157803A1 PURIFICATION OF AMPHOTERIC PRODUCTS 审中-公开
标题翻译：净化产品
公开(公告)号：WO2011157803A1
公开(公告)日：2011-12-22
申请号：PCT/EP2011/060058
申请日：2011-06-16
申请人： CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE , UNIVERSITE DE REIMS CHAMPAGNE-ARDENNE , LONZA AG , GIRAUD, Matthieu , MCGARRITY, John , RENAULT, Jean-Hugues , BOUDESOCQUES, Leslie
发明人： GIRAUD, Matthieu , MCGARRITY, John , RENAULT, Jean-Hugues , BOUDESOCQUES, Leslie
IPC分类号： C07K5/06 , C07K14/415 , C07K14/47
CPC分类号： C07K5/06026 , B01D15/1892 , B01D15/422 , C07K5/06043 , C07K5/06052 , G01N30/42
摘要： The invention relates to the use of centrifugal partition chromatography in an ion exchange displacement mode for the implementation of a purification process of at least one product to be purified from a substrate containing it, said product to be purified being an amphoteric product or an amphoteric convertible product.
摘要翻译：本发明涉及离子分配色谱法在离子交换置换模式中的用途，用于从含有其的底物中纯化至少一种待纯化的产物的纯化方法，待纯化的产物是两性产物或两性可转换产品。

3. 发明申请

WO9913330A3 PURIFICATION OF SUBSTANCES FROM A BIOLOGICAL SAMPLE 审中-公开
标题翻译： ESSENCE从生物样品纯化
公开(公告)号：WO9913330A3
公开(公告)日：1999-06-17
申请号：PCT/EP9805521
申请日：1998-08-31
申请人： ROCHE DIAGNOSTICS GMBH , HOESEL WOLFGANG , LENZ HELMUT , PETER JOCHEN
发明人： HOESEL WOLFGANG , LENZ HELMUT , PETER JOCHEN
IPC分类号： G01N33/53 , B01D15/00 , B01D15/08 , C07K1/22 , C12S3/00 , G01N33/00 , G01N33/543
CPC分类号： C07K1/22 , B01D15/3804 , B01D15/422 , Y10S435/803 , Y10S435/81 , Y10S435/814 , Y10S435/815 , Y10S435/971 , Y10S435/975 , Y10S436/823 , Y10S436/824
摘要： The invention relates to a method for purifying a target substance from a biological sample, involving immobilisation of the target substance on a solid phase through a high affinity binding pair, and follow-on elution through addition of a partner of the binding pair in a free form. The invention further relates to reagent kits for carrying out the inventive method.
摘要翻译：本发明涉及一种方法，用于通过经由高亲和力通过加入游离形式的结合对的配偶体结合对和后续洗脱固定在固相上的目标物质纯化从生物样品中的目标物质。此外试剂盒公开了用于实施该方法。

4. 发明申请

WO1995022555A1 DISPLACEMENT CHROMATOGRAPHY OF PROTEINS USING LOW MOLECULAR WEIGHT DISPLACERS 审中-公开
标题翻译：使用低分子重量位移的蛋白质的位移色谱
公开(公告)号：WO1995022555A1
公开(公告)日：1995-08-24
申请号：PCT/US1995001966
申请日：1995-02-16
申请人： CRAMER, Steven, M. , MOORE, James, A.
发明人： KUNDU, Amitava , LI, Yufei , JAYARAMAN, Guhan
IPC分类号： C07K01/14
CPC分类号： B01D15/422 , C07C215/40 , C07K1/18 , G01N30/02
摘要： A method for the purification of proteins by displacement chromatography on ion exchange media using molecular weight displacers is disclosed. Several classes of low molecular weight, charged species are exemplified, including aminoacids, peptides, antibiotics and dendrimeric polymers. Novel compounds useful as displacers are dendrimers of formula (I), wherein R is lower alkyl, n is 2 to 6, and X is a common counter anion and similar dendritic polymers based thereon.
摘要翻译：公开了一种使用分子量置换器在离子交换介质上通过置换色谱法纯化蛋白质的方法。例举了几类低分子量带电物质，包括氨基酸，肽，抗生素和树枝状聚合物。用作置换剂的新型化合物是式（I）的树枝状大分子，其中R 1是低级烷基，n是2-6，X是常见的抗衡阴离子和基于其的类似树枝状聚合物。

5. 发明申请

WO1992019767A1 METHODS TO PROFILE AND TREAT ABNORMAL CELLS 审中-公开
标题翻译：描述和治疗异常细胞的方法
公开(公告)号：WO1992019767A1
公开(公告)日：1992-11-12
申请号：PCT/US1992003537
申请日：1992-04-29
申请人： TERRAPIN TECHNOLOGIES, INC. , KAUVAR, Lawrence, M.
发明人： TERRAPIN TECHNOLOGIES, INC.
IPC分类号： C12Q01/48
CPC分类号： G01N33/573 , G01N30/02 , G01N33/57496 , G01N2333/91177 , B01D15/3804 , B01D15/422
摘要： Advantage is taken of identifying the differences in glutathione-S-transferase (GST) complements of unwanted tissues and cells as compared to normal tissues and cells to evaluate therapeutic protocols designed to treat conditions characterized by, for example, tumor cells or infectious agents. Therapeutic modulators which affect preferentially the GST complement of normal or unwanted cells or tissues can thereby also be selected for use in combination with drugs which serve as substrates for GST.
摘要翻译：与正常组织和细胞相比，鉴定与不正常组织和细胞的谷胱甘肽-S-转移酶（GST）互补物的差异的优点是为了评估设计用于治疗特征在于例如肿瘤细胞或感染因子的病症的治疗方案。因此，优选影响正常或不需要的细胞或组织的GST补体的治疗性调节剂也可以选择与用作GST的底物的药物组合使用。

6. 发明申请

WO1991006356A1 METHOD TO IDENTIFY ANALYTE-BINDING LIGANDS 审中-公开
标题翻译：识别分析结合配体的方法
公开(公告)号：WO1991006356A1
公开(公告)日：1991-05-16
申请号：PCT/US1990006333
申请日：1990-10-31
申请人： TERRAPIN TECHNOLOGIES, INC.
发明人： TERRAPIN TECHNOLOGIES, INC. , KAUVAR, Lawrence, M.
IPC分类号： B01D15/08
CPC分类号： C07K7/06 , B01D15/08 , B01J20/264 , B01J20/28033 , B01J20/286 , B01J20/289 , B01J20/29 , B01J20/3204 , B01J20/3208 , B01J20/3219 , B01J20/3274 , B01J20/328 , B01J20/3285 , B01J2220/58 , B01J2220/66 , C07K1/22 , C07K7/56 , C07K16/44 , G01N30/00 , G01N30/02 , G01N30/48 , G01N33/53 , G01N33/68 , G01N2030/027 , B01D15/422 , B01D15/3804
摘要： The invention provides methods to obtain panels of low molecular weight polymers resulting from varying constituent monomers, which panels are systematically diverse with respect to two or more variables. The panels can be screened for members (paralogs) which are capable of specifically binding a target moiety. Such paralogs are useful for chromatographic separations and purifications of desired analytes and in binding assays, such as immunoassays, involving said analytes, as well as for any purpose which requires said specific binding. The invention also provides kits for these determinations, and methods to synthesize panels of candidate paralogs and use of these panels to generate analyte profiles and cross reactivity matrices.
摘要翻译：本发明提供了获得由不同构成单体产生的低分子量聚合物的面板的方法，该面板相对于两个或多个变量系统地多样化。可以对能够特异性结合目标部分的成员（旁系同源物）进行筛选。这样的旁系同源物可用于所需分析物的色谱分离和纯化以及结合测定，例如涉及所述分析物的免疫测定，以及需要所述特异性结合的任何目的。本发明还提供用于这些测定的试剂盒，以及合成候选旁系同源板的方法以及使用这些面板产生分析物谱和交叉反应性基质。

7. 发明申请

WO1991003298A1 CONTINUOUS HIGH PERFORMANCE LIQUID CHROMATOGRAPHY 审中-公开
标题翻译：连续高性能液相色谱
公开(公告)号：WO1991003298A1
公开(公告)日：1991-03-21
申请号：PCT/US1990004865
申请日：1990-08-27
申请人： UNION OIL COMPANY OF CALIFORNIA
发明人： UNION OIL COMPANY OF CALIFORNIA , TANIGUCHI, Vernon, T. , BOSSERMAN, Paula, J. , DOTY, Allen, W.
IPC分类号： B01D15/08
CPC分类号： B01D15/1892 , G01N30/02 , G01N2030/587 , B01D15/422
摘要： Chromatographic species are continuously separated by either elution or displacement HPLC using an apparatus (20) that comprises a stationary inlet distribution manifold (22), a stationary chromatographic bed (38), and mobile collector receptacles (26).

8. 发明申请

WO1990010873A1 BUFFER AND REAGENT SYSTEM FOR SEPARATING AMINO ACIDS 审中-公开
标题翻译：用于分离氨基酸的缓冲剂和试剂体系
公开(公告)号：WO1990010873A1
公开(公告)日：1990-09-20
申请号：PCT/EP1990000384
申请日：1990-03-09
申请人： MERCK PATENT GESELLSCHAFT MIT BESCHRÄNKTER ... , KABUS, Hans-Peter
发明人： MERCK PATENT GESELLSCHAFT MIT BESCHRÄNKTER ...
IPC分类号： G01N33/68
CPC分类号： B01D15/362 , B01D15/422 , B01J39/26 , G01N30/02 , B01D15/36
摘要： The invention relates to a citrate free and chloride-free buffer and reagent system for the separation of amino acids and other ninhydrin-positive components of cation exchangers and their quantitative determination. The buffer and reagent system does not attack special steels and is suitable for amino acid analysers and especially for high-pressure liquid chromatography installations.

9. 发明申请

WO2013052087A1 NEUTRAL ZWITTERIONIC DISPLACER MOLECULES FOR HYDROPHOBIC DISPLACEMENT CHROMATOGRAPHY 审中-公开
标题翻译：用于疏水性位移色谱法的中性ZWITTERIONIC位移分子
公开(公告)号：WO2013052087A1
公开(公告)日：2013-04-11
申请号：PCT/US2012/000433
申请日：2012-10-03
申请人： SACHEM, INC.
发明人： HAYMORE, Barry, L.
IPC分类号： B01D15/32 , B01D15/42
CPC分类号： C07K1/20 , B01D15/325 , B01D15/422
摘要： A process for separating organic compounds from a mixture by reverse- phase displacement chromatography, including providing a hydrophobic stationary phase; applying to the hydrophobic stationary phase a mixture comprising organic compounds to be separated; displacing the organic compounds from the hydrophobic stationary phase by applying thereto an aqueous composition comprising a non-surface active hydrophobic neutral zwitterionic displacer molecule and optionally an organic solvent; and collecting a plurality of fractions eluted from the hydrophobic stationary phase containing the separated organic compounds; in which the non-surface active hydrophobic neutral zwitterionic displacer molecule comprises a hydrophobic zwitterion having the general formula, as defined in the disclosure: [CM-R*-CM'].
摘要翻译：通过反相置换色谱从混合物中分离有机化合物的方法，包括提供疏水性固定相; 向疏水性固定相施加包含要分离的有机化合物的混合物; 通过向其施加包含非表面活性的疏水性中性两性离子置换剂分子和任选的有机溶剂的水性组合物来从疏水性固定相中移出有机化合物; 并收集从含有分离的有机化合物的疏水性固定相洗脱的多个级分; 其中非表面活性疏水性中性两性离子置换剂分子包含如本公开所定义的具有通式的疏水性两性离子：[CM-R * -CM']。

10. 发明申请

WO2012068134A1 ENRICHMENT AND CONCENTRATION OF SELECT PRODUCT ISOFORMS BY OVERLOADED BIND AND ELUTE CHROMATOGRAPHY 审中-公开
标题翻译：通过超载和ELVTE色谱法选择产品ISOFORMS的增强和浓缩
公开(公告)号：WO2012068134A1
公开(公告)日：2012-05-24
申请号：PCT/US2011/060821
申请日：2011-11-15
申请人： BIOGEN IDEC INC. , LADIWALA, Asif , PIERACCI, John
发明人： LADIWALA, Asif , PIERACCI, John
IPC分类号： B01D15/42
CPC分类号： C07K1/22 , B01D15/361 , B01D15/3804 , B01D15/3847 , B01D15/422
摘要： Disclosed is a method for enhancing or increasing the concentration of biological product in a final mixture, wherein said biological product has one or more selected characteristics, wherein said method comprises: (a) allowing an initial mixture of biological products with and without said selected characteristics to contact a chromatography medium wherein the quantity of biological products in said initial mixture exceeds the binding capacity or the dynamic binding capacity of said chromatography medium; (b) allowing biological product not having said one or more selected characteristics to be separated by said chromatography medium; and (c) recovering a final mixture of biological products from said chromatography medium wherein said final mixture comprises an enhanced or increased concentration of biological product with one or more selected characteristics, compared to the concentration of biological product in said initial mixture.
摘要翻译：公开了一种用于增强或增加最终混合物中生物产物浓度的方法，其中所述生物产物具有一个或多个所选择的特征，其中所述方法包括：（a）允许具有和不具有所述选定特征的生物产物的初始混合接触色谱介质，其中所述初始混合物中生物产物的量超过所述色谱介质的结合能力或动态结合能力; （b）允许不具有所述一种或多种所选特征的生物制品通过所述色谱介质分离; 和（c）从所述色谱介质中回收生物产物的最终混合物，其中与所述初始混合物中生物产物的浓度相比，所述最终混合物包含具有一种或多种所选特征的增强或增加浓度的生物产物。

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