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21. 发明申请

WO2010051978A1 SUPPRESSION OF SECONDARY CAPTURE IN MICROARRAY ASSAYS 审中-公开
标题翻译：在微量测定中抑制二次捕获
公开(公告)号：WO2010051978A1
公开(公告)日：2010-05-14
申请号：PCT/EP2009/007898
申请日：2009-11-04
申请人： ROCHE DIAGNOSTICS GMBH , F. HOFFMANN-LA ROCHE AG
发明人： ALBERT, Thomas , JEDDELOH, Jeff , SWANSON, Bradley
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6837 , C12Q1/6832 , C12Q2537/163 , C12Q2525/191 , C12Q2525/151 , C12Q2549/126
摘要： The present invention provides for compositions, methods and systems for targeted sequence enrichment. In particular, the present invention provides for enriching for targeted nucleic acid sequences during hybridizations in microarray assays by suppressing secondary capture of non-target nucleic acid sequences.
摘要翻译：本发明提供用于靶向序列富集的组合物，方法和系统。特别地，本发明提供了通过抑制非靶核酸序列的二次捕获在微阵列测定中杂交期间富集目标核酸序列。

22. 发明申请

WO2009048928A1 METHODS, COMPOSITIONS AND KITS FOR THE IMPROVED DETECTION OF SMALL RNA MOLECULES 审中-公开
标题翻译：用于改进小RNA分子检测的方法，组合物和试剂盒
公开(公告)号：WO2009048928A1
公开(公告)日：2009-04-16
申请号：PCT/US2008/079169
申请日：2008-10-08
申请人： KUTYAVIN, Igor, Vassily
发明人： KUTYAVIN, Igor, Vassily
IPC分类号： C12Q1/68
CPC分类号： C12Q1/686 , C12Q1/6832 , C12Q1/6853 , C12Q2521/107 , C12Q2525/117 , C12Q2525/207
摘要： The present invention provides compositions, methods and kits for use in the detection of small RNA sequences, which allow for rapid and robust amplification and detection. The methods provide improved sensitivity and efficiency in the amplification-based detection of small RNA sequences by incorporating one or more base-modified duplex-stabilizing dNTPs during reverse transcription and/or amplification.
摘要翻译：本发明提供用于检测小RNA序列的组合物，方法和试剂盒，其允许快速和鲁棒的扩增和检测。该方法通过在逆转录和/或扩增过程中掺入一个或多个碱基修饰的双稳态dNTPs来提供基于扩增的小RNA序列检测的灵敏度和效率。

23. 发明申请

WO2009035554A2 SUPERIOR STRUCTURE STABILITY AND SELECTIVITY OF HAIRPIN NUCLEIC ACID PROBES WITH AN L-DNA STEM 审中-公开
标题翻译：具有L-DNA STEM的头发核酸探针的优良结构稳定性和选择性
公开(公告)号：WO2009035554A2
公开(公告)日：2009-03-19
申请号：PCT/US2008/010466
申请日：2008-09-08
申请人： UNIVERSITY OF FLORIDA RESEARCH FOUNDATION, INC. , TAN, Weihong , KIM, Youngmi , YANG, Chaoyong, James
发明人： TAN, Weihong , KIM, Youngmi , YANG, Chaoyong, James
IPC分类号： C12N15/09 , C12M1/00 , C12Q1/68 , G01N33/58
CPC分类号： C12Q1/6832 , C12Q2525/301 , C12Q2525/101
摘要： Hairpin-shaped nucleic acid probes capable of forming stable hybrids with target nucleic acid sequences are provided. In particular, an oligonucleotide probe for hybridization analysis is provided, wherein the probe comprises a non-natural enantiomeric DNA (L-DNA) that forms the stem and natural DNA (D-DNA) that forms the loop in the hairpin. The invention thereby provides stable probes that experience fewer stem invasions and demonstrate sensitive and selective hybridization to target nucleic acid sequences. The subject probes are useful for a variety of biological and biotechnological applications.
摘要翻译：提供了能够与靶核酸序列形成稳定杂交体的发夹形核酸探针。具体而言，提供了用于杂交分析的寡核苷酸探针，其中探针包含形成茎的非天然对映体DNA（L-DNA）和形成发夹中环的天然DNA（D-DNA）。由此，本发明提供了经历较少茎侵入并证明对靶核酸序列敏感和选择性杂交的稳定探针。主题探针可用于各种生物和生物技术应用。

24. 发明申请

WO2008142571A2 PROBE FOR DETECTING A PARTICULAR NUCLEIC ACID SEQUENCE 审中-公开
标题翻译：用于检测特定核酸序列的探针
公开(公告)号：WO2008142571A2
公开(公告)日：2008-11-27
申请号：PCT/IB2008/002111
申请日：2008-05-21
申请人： HUMBOLDT-UNIVERSITÄT ZU BERLIN , SEITZ, Oliver , GROSSMANN, Tom, N. , RÖGLIN, Lars
发明人： SEITZ, Oliver , GROSSMANN, Tom, N. , RÖGLIN, Lars
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6818 , C12Q1/6832 , C12Q2537/119 , C12Q2525/301
摘要： The invention relates to a detection molecule (triplex Molecular Beacon, tMB) for detecting a particular nucleic acid sequence, in particular for detecting a single nucleotide mismatch in a target nucleic acid. Such a detection molecule comprises a) an oligonucleotide, and b) a probe comprising a first stem forming portion with a fluorophore for generating a detection signal, a second stem forming portion with a quencher for quenching the detection signal from the fluorophore when the quencher is in proximity to the fluorophore, and a loop forming portion located between the first and the second stem forming portion, for allowing the first stem forming portion to form a stem structure together with the second stem forming portion of the probe and with the oligonucleotide. According to the invention, the oligonucleotide comprises a portion allowing it to form a triplex structure with the first and second stem forming portions of the probe.
摘要翻译：本发明涉及用于检测特定核酸序列的特异性检测分子（三重分子信标（triplex Molecular Beacon，tMB）），特别是用于检测目标核酸中的单核苷酸错配。这样的检测分子包含：a）寡核苷酸，和b）包含具有产生检测信号的荧光团的第一茎形成部分的探针，当猝灭剂为猝灭剂时，具有猝灭剂的第二茎形成部分用于猝灭来自荧光团的检测信号位于第一和第二茎形成部分之间的环形部分，用于允许第一茎形成部分与探针的第二茎形成部分和寡核苷酸一起形成茎结构。根据本发明，寡核苷酸包括允许其与探针的第一和第二茎形成部分形成三重结构的部分。

25. 发明申请

WO2007127992A3 USE OF BASE-MODIFIED DEOXYNUCLEOSIDE TRIPHOSPHATES 审中-公开
标题翻译：碱改性脱氧核糖核酸二磷酸酯的使用
公开(公告)号：WO2007127992A3
公开(公告)日：2008-11-13
申请号：PCT/US2007067826
申请日：2007-04-30
申请人： KUTYAVIN IGOR
发明人： KUTYAVIN IGOR
IPC分类号： C12P19/34 , C12Q1/68
CPC分类号： C12Q1/686 , C12Q1/6832 , C12Q2527/125 , C12Q2527/107 , C12Q2525/117 , C12Q2525/101
摘要： Aspects of the invention provide novel and surprisingly effective methods for the detection of nucleic acids, comprising nucleic acid amplification using base-modified deoxynucleoside 5'-triphosphates (dNTPs). Particular aspects relate to methods for enhancing hybridization properties of oligonucleotide primers and probes in assays detecting nucleic acids, comprise amplifying target DNAs in presence of base-modified duplex-stabilizing deoxyribonucleoside 5'-triphosphates to provide for modified target DNAs, and thereby considerably improving performance of the detection assays. The disclosed methods allow for increasing of the reaction temperature in PCR-based detection systems or, alternatively, reducing the length of the oligonucleotide primers and probes. Certain aspects relates to improvement of real time PCR assays, wherein nucleic acids of interest are detected as the reaction proceeds using fluorescent agents or oligonucleotide FRET probes.
摘要翻译：本发明的方面提供了用于检测核酸的新颖且令人惊奇的有效方法，其包括使用碱基修饰的脱氧核苷5'-三磷酸（dNTP）的核酸扩增。特定方面涉及在检测核酸的检测检测中增强寡核苷酸引物和探针的杂交性质的方法，包括在碱修饰的双链稳定化脱氧核糖核苷5'-三磷酸存在下扩增靶DNA以提供修饰的靶DNA，从而显着提高性能的检测分析。所公开的方法允许在基于PCR的检测系统中增加反应温度，或者替代地减少寡核苷酸引物和探针的长度。某些方面涉及实时PCR测定的改进，其中当使用荧光剂或寡核苷酸FRET探针进行反应时，检测到目标核酸。

26. 发明申请

WO2008029295A2 A METHOD FOR DETECTING NUCLEIC ACIDS 审中-公开
标题翻译：一种检测核酸的方法
公开(公告)号：WO2008029295A2
公开(公告)日：2008-03-13
申请号：PCT/IB2007/003732
申请日：2007-06-29
申请人： ROSETTA GENOMICS LTD , SHLOMIT, Gilad , MEIRI, Esther , YERUSHALMI, Noga
发明人： SHLOMIT, Gilad , MEIRI, Esther , YERUSHALMI, Noga
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6876 , C12Q1/6816 , C12Q1/6832 , C12Q1/6853 , C12Q1/6886 , C12Q2600/136 , C12Q2600/178 , C12Q2545/101 , C12Q2563/149 , C12Q2565/501 , C12Q2565/626 , C12Q2521/107 , C12Q2525/207 , C12Q2525/301 , C12Q2561/101 , C12Q2525/161
摘要： Described herein are methods and compositions for detecting, amplifying and labeling targeted nucleic acids, including microRNAs.
摘要翻译：本文描述了用于检测，扩增和标记靶向核酸（包括微小RNA）的方法和组合物。

27. 发明申请

WO2007090345A1 HYBRIDIZATION METHODS USING NATURAL BASE MISMATCHES 审中-公开
标题翻译：使用自然基因杂交的杂交方法
公开(公告)号：WO2007090345A1
公开(公告)日：2007-08-16
申请号：PCT/CN2007/000402
申请日：2007-02-06
申请人： CAPITALBIO CORPORATION , TSINGHUA UNIVERSITY , GAO, Huafang , LI, Ze , WANG, Dong , LIU, Yanhua , LIU, Xiang , JIANG, Yangzhou , LI, Li , ZHAO, Chuanzan , LAN, Gengxin , GUO, Tao , CAI, Bin , CHENG, Jing
发明人： GAO, Huafang , LI, Ze , WANG, Dong , LIU, Yanhua , LIU, Xiang , JIANG, Yangzhou , LI, Li , ZHAO, Chuanzan , LAN, Gengxin , GUO, Tao , CAI, Bin , CHENG, Jing
IPC分类号： C12Q1/68 , C12N15/00
CPC分类号： C12Q1/6881 , C12Q1/6832 , C12Q2600/156 , C12Q2535/131 , C12Q2527/107
摘要： The present invention provides an improved nucleic acid hybridization process employing a modified oligonucleotide probe comprising naturally occurring nucleotide bases. At least one nucleotide in the modified oligonucleotide is artificially mismatched relative to the control nucleic acid in addition to any mismatches arising from a variant nucleic acid target containing a sequence variation. The artificial mismatch and the sequence variation positions are separated from one another on the oligonucleotide by six to nine nucleotide positions.
摘要翻译：本发明提供使用包含天然存在的核苷酸碱基的修饰的寡核苷酸探针的改进的核酸杂交方法。除了由含有序列变异的变体核酸靶产生的任何错配之外，修饰寡核苷酸中至少一个核苷酸相对于对照核酸人为错配。在寡核苷酸上将人工错配和序列变异位置彼此分开6至9个核苷酸位置。

28. 发明申请

WO2007073166A1 IMPROVED TARGETED NUCLEOTIDE EXCHANGE WITH PROPYNYL MODIFIED OLIGONUCLEOTIDES 审中-公开
标题翻译：用改性的寡核苷酸改进靶向的核苷酸交换
公开(公告)号：WO2007073166A1
公开(公告)日：2007-06-28
申请号：PCT/NL2006/000649
申请日：2006-12-20
申请人： KEYGENE N.V. , BUNDOCK, Paul , DE BOTH, Michiel, Theodoor, Jan , HOGERS, René, Cornelis, Josephus
发明人： BUNDOCK, Paul , DE BOTH, Michiel, Theodoor, Jan , HOGERS, René, Cornelis, Josephus
IPC分类号： C12N15/10
CPC分类号： C12Q1/6816 , C12N9/88 , C12N15/102 , C12N15/8213 , C12N15/8274 , C12N15/8278 , C12Q1/6827 , C12Q1/6832 , C12Q2527/107 , C12Q2525/113 , C12Q2525/117
摘要： A method and oligonucleotides for targeted nucleotide exchange of a duplex DNA sequence, wherein the donor oligonucleotide contains at least one modified nucleotide which is a propynylated purine and/or pyrimidine having a higher binding affinity compared to naturally occurring A, C, T or G and/or binds stronger to a nucleotide in an opposite position in the first DNA sequence as compared to a naturally occurring nucleotide complementary to the nucleotide in the opposite position in the first DNA sequence.
摘要翻译：用于双链体DNA序列靶向核苷酸交换的方法和寡核苷酸，其中与天然存在的A，C，T或G相比，供体寡核苷酸含有至少一个经修饰的核苷酸，其是具有较高结合亲和力的丙炔基化嘌呤和/或嘧啶， /或与第一DNA序列中相对位置的核苷酸互补的天然存在的核苷酸相比，与第一DNA序列相对位置的核苷酸更强。

29. 发明申请

WO2007073154A1 ALTERNATIVE NUCLEOTIDES FOR IMPROVED TARGETED NUCLEOTIDE EXCHANGE 审中-公开
标题翻译：用于改善靶向核苷酸交换的替代核苷酸
公开(公告)号：WO2007073154A1
公开(公告)日：2007-06-28
申请号：PCT/NL2006/000244
申请日：2006-05-09
申请人： KEYGENE N.V. , DE BOTH, Michel, Theodoor, Jan , BUNDOCK, Paul , HOGERS, René, Cornelis, Josephus
发明人： DE BOTH, Michel, Theodoor, Jan , BUNDOCK, Paul , HOGERS, René, Cornelis, Josephus
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6816 , C12N9/88 , C12N15/102 , C12N15/8213 , C12N15/8274 , C12N15/8278 , C12Q1/6827 , C12Q1/6832 , C12Q2527/107 , C12Q2525/113 , C12Q2525/117
摘要： A method and oligonucleotides for targeted nucleotide exchange of a duplex DNA sequence, wherein the donor oligonucleotide contains at least one modified nucleotide having a higher binding affinity compared to naturally occurring A, C, T or G and/or binds stronger to a nucleotide in an opposite position in the first DNA sequence as compared to a naturally occurring nucleotide complementary to the nucleotide in the opposite position in the first DNA sequence.
摘要翻译：用于双链DNA序列的靶向核苷酸交换的方法和寡核苷酸，其中所述供体寡核苷酸包含至少一个与天然存在的A，C，T或G相比具有更高结合亲和力的修饰核苷酸和/ 或与第一DNA序列中与相反位置的核苷酸互补的天然存在的核苷酸相比，第一DNA序列中相对位置的核苷酸结合更强。

30. 发明申请

WO2007031874A2 METHOD OF SIMULTANEOUSLY VISUALIZING MULTIPLE BIOLOGICAL TARGETS 审中-公开
标题翻译：同时可视化多个生物目标的方法
公开(公告)号：WO2007031874A2
公开(公告)日：2007-03-22
申请号：PCT/IB2006/003126
申请日：2006-06-30
申请人： DAKO DENMARK A/S , WINTHER, Lars
发明人： WINTHER, Lars
CPC分类号： G01N33/5306 , C12Q1/6832 , G01N33/54306 , G01N33/54393 , Y10T436/143333 , C12Q2523/101
摘要： The present invention relates to optionally automated methods that may be used to qualitatively and/or quantitatively detect at least one, or for example, two or more different targets in a sample, and kits associated with such methods. The two or more different targets may be detected and distinguished by adding at least one cross-linking agent to the sample in between different steps of a detection procedure. The addition of a cross-linking agent may allow for drastic changes in buffer conditions (i.e. solvent, pH, salt concentration, etc.) or temperature in order to refine a detection procedure with minimal loss of signal. The instant invention is compatible with a variety of detection systems, including immunohistochemistry (IHC), immunocytochemistry (ICC), in situ hybridization (ISH), flow cytometry, enzyme immuno-assays (EIA), enzyme linked immuno- assays (ELISA), blotting methods (e.g. Western, Southern, and Northern), labeling inside electrophoresis systems or on surfaces or arrays, and precipitation, among other general detection assay formats. The invention is also compatible with many different types of samples, targets, probes, and detectable labels.
摘要翻译：本发明涉及可以用于定性和/或定量检测样品中至少一种或者例如两种或更多种不同目标的任选自动化方法，以及与这种方法相关的试剂盒。可以通过在检测程序的不同步骤之间向样品添加至少一种交联剂来检测和区分两种或更多种不同的目标。交联剂的添加可以允许缓冲条件（即溶剂，pH，盐浓度等）或温度的剧烈变化，以便在信号损失最小的情况下完善检测程序。本发明与各种检测系统兼容，包括免疫组织化学（IHC），免疫细胞化学（ICC），原位杂交（ISH），流式细胞术，酶免疫测定法（EIA），酶联免疫测定法（ELISA），印迹方法（例如Western，Southern和Northern），在电泳系统或表面或阵列内标记，以及沉淀，以及其他常规检测测定形式。本发明还与许多不同类型的样本，目标，探针和可检测标签兼容。

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