基本信息:
- 专利标题： METHOD OF SIMULTANEOUSLY VISUALIZING MULTIPLE BIOLOGICAL TARGETS
- 专利标题（中）：同时可视化多个生物目标的方法
- 申请号：PCT/IB2006/003126 申请日：2006-06-30
- 公开(公告)号：WO2007031874A2 公开(公告)日：2007-03-22
- 发明人： WINTHER, Lars
- 申请人： DAKO DENMARK A/S , WINTHER, Lars
- 申请人地址： Produktionsvej 42, DK-2600 Glostrup DK
- 专利权人： DAKO DENMARK A/S,WINTHER, Lars
- 当前专利权人： DAKO DENMARK A/S,WINTHER, Lars
- 当前专利权人地址： Produktionsvej 42, DK-2600 Glostrup DK
- 优先权： US60/695,410 20050701
摘要：
The present invention relates to optionally automated methods that may be used to qualitatively and/or quantitatively detect at least one, or for example, two or more different targets in a sample, and kits associated with such methods. The two or more different targets may be detected and distinguished by adding at least one cross-linking agent to the sample in between different steps of a detection procedure. The addition of a cross-linking agent may allow for drastic changes in buffer conditions (i.e. solvent, pH, salt concentration, etc.) or temperature in order to refine a detection procedure with minimal loss of signal. The instant invention is compatible with a variety of detection systems, including immunohistochemistry (IHC), immunocytochemistry (ICC), in situ hybridization (ISH), flow cytometry, enzyme immuno-assays (EIA), enzyme linked immuno- assays (ELISA), blotting methods (e.g. Western, Southern, and Northern), labeling inside electrophoresis systems or on surfaces or arrays, and precipitation, among other general detection assay formats. The invention is also compatible with many different types of samples, targets, probes, and detectable labels.
摘要（中）：
本发明涉及可以用于定性和/或定量检测样品中至少一种或者例如两种或更多种不同目标的任选自动化方法，以及与这种方法相关的试剂盒 。 可以通过在检测程序的不同步骤之间向样品添加至少一种交联剂来检测和区分两种或更多种不同的目标。 交联剂的添加可以允许缓冲条件（即溶剂，pH，盐浓度等）或温度的剧烈变化，以便在信号损失最小的情况下完善检测程序。 本发明与各种检测系统兼容，包括免疫组织化学（IHC），免疫细胞化学（ICC），原位杂交（ISH），流式细胞术，酶免疫测定法（EIA），酶联免疫测定法（ELISA）， 印迹方法（例如Western，Southern和Northern），在电泳系统或表面或阵列内标记，以及沉淀，以及其他常规检测测定形式。 本发明还与许多不同类型的样本，目标，探针和可检测标签兼容。