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11. 发明申请

WO2010022441A1 EXTRACTION PROCESSES 审中-公开
标题翻译：提取过程
公开(公告)号：WO2010022441A1
公开(公告)日：2010-03-04
申请号：PCT/AU2009/001086
申请日：2009-08-25
申请人： UNIVERSITY OF SOUTH AUSTRALIA , RALSTON, John , PRIEST, Craig, Ian , SEDEV, Rossen, Velizarov , MAWATARI, Kazuma , KITAMORI, Takehiko
发明人： RALSTON, John , PRIEST, Craig, Ian , SEDEV, Rossen, Velizarov , MAWATARI, Kazuma , KITAMORI, Takehiko
IPC分类号： B01D11/04
CPC分类号： G01N1/4055 , B01D11/04 , B01D11/0492 , B01D11/0496 , B01L3/5027 , B01L3/502753 , G01N2001/4061 , G01N2001/4072
摘要： A process for extracting a solute from an analyte-containing fluid phase. The process includes passing the analyte-containing fluid phase along a first fluid microchannel of a microfluidic extraction device and passing an extractant fluid phase that is at least partially immiscible with the analyte-containing fluid phase along a second fluid microchannel of the microfluidic extraction device. The analyte-containing fluid phase and the extractant fluid phase contact one another at a contact zone formed between the first and second fluid microchannels so that the solute is able to diffuse from the analyte-containing fluid phase into the extractant fluid phase. The analyte-containing fluid phase and/or the extractant fluid phase is a particle laden phase.
摘要翻译：从含分析物的流体相萃取溶质的方法。该方法包括沿着微流体提取装置的第一流体微通道传送含分析物的流体相，并且使得与微流体提取装置的第二流体微通道至少部分地不与含分析物的流体相混溶的萃取剂流体相。含分析物的流体相和萃取液相在第一和第二流体微通道之间形成的接触区彼此接触，使得溶质能够从含分析物的流体相扩散到萃取剂流体相中。含分析物的流体相和/或萃取剂流体相是载有颗粒的相。

12. 发明申请

WO2009048533A2 REACTIONS WITHIN MICROFLUIDIC CHANNELS 审中-公开
标题翻译：微流控通道内的反应
公开(公告)号：WO2009048533A2
公开(公告)日：2009-04-16
申请号：PCT/US2008/011457
申请日：2008-10-03
申请人： PRESIDENT AND FELLOWS OF HARVARD COLLEGE , STONE, Howard, A. , RISTENPART, William, D. , WAN, Jiandi
发明人： STONE, Howard, A. , RISTENPART, William, D. , WAN, Jiandi
IPC分类号： B01J19/00 , B01L3/00
CPC分类号： B01J19/0093 , B01J2219/00783 , B01J2219/00824 , B01J2219/00828 , B01J2219/00831 , B01J2219/00833 , B01J2219/00837 , B01J2219/0086 , B01J2219/00889 , B01J2219/00891 , B01J2219/00957 , B01J2219/0097 , B01J2219/00986 , B01L3/502776 , B01L2300/0867 , B01L2400/0487 , G01N1/38 , G01N2001/4072
摘要： The present invention generally relates to microfluidics and, in particular, to microfluidic systems useful for determining reactions. In one aspect, two or more fluids are introduced into a microfluidic channel, and are allowed to come into contact. At or near the interface between the fluids, reactants contained within the fluids may react. By determining the reaction rate between the reactants with respect to position within the microfluidic channel, information about the reaction between the reactants can be obtained. The reaction rate may be determined, for instance, by measuring the rate of reaction at two or more points within the channel. In some cases, e.g., if the reaction rate can be determined optically or visually, the channel may be imaged and the image analyzed to determine the reaction rate. As a non-limiting example of a reaction, the reactants may be an enzyme and a substrate, and by determining reaction rates within the channel, Michaelis-Menten kinetics (or other reaction kinetics) of the enzymatic reaction may be determined. It should be understood, however, that other reactions besides Michaelis-Menten kinetics and/or enzymatic reactions may also be determined, including other catalytic or chemical reactions, and the like. The reaction profiles may be linear or non-linear, e.g., second order, third order, etc.
摘要翻译：本发明一般涉及微流体，并且特别涉及用于确定反应的微流体系统。在一个方面，两种或更多种流体被引入微流体通道中，并且被允许接触。在流体之间的界面处或附近，包含在流体内的反应物可以反应。通过确定反应物之间关于微流体通道内位置的反应速率，可以获得关于反应物之间反应的信息。反应速率可以通过例如测量通道内两个或更多点的反应速率来确定。在一些情况下，例如如果可以光学或视觉确定反应速率，则可以对通道进行成像并分析图像以确定反应速率。作为反应的非限制性实例，反应物可以是酶和底物，并且通过确定通道内的反应速率，可以确定酶促反应的米氏（Michaelis-Menten）动力学（或其他反应动力学）。然而，应该理解的是，除Michaelis-Menten动力学和/或酶促反应之外，还可以确定其他反应，包括其他催化或化学反应等。反应曲线可以是线性的或非线性的，例如二阶，三阶等。

13. 发明申请

WO2008148028A3 METHODS AND APPARATUSES FOR SEPARATING BIOLOGICAL PARTICLES 审中-公开
标题翻译：分离生物颗粒的方法和装置
公开(公告)号：WO2008148028A3
公开(公告)日：2009-02-05
申请号：PCT/US2008064747
申请日：2008-05-23
申请人： APPLERA CORP , LIU YINGJIE
发明人： LIU YINGJIE
IPC分类号： C12N5/06 , C12N5/00
CPC分类号： B03C1/32 , B01L3/502753 , B01L2400/0409 , B01L2400/043 , B01L2400/0487 , G01N1/4055 , G01N2001/4072
摘要： Methods and apparatuses for separating biological particles are provided In certain embodiments the separation apparatus is utilized for separating first biological particles from second biological particles under flow conditions, and comprises (a) a separation region, b) a first inlet port operably connected to a first end of the separation region, c) a second inlet port operably connected to the first end of the separation region, d) two or more posts aligned along a plane extending along a diagonal axis from the first end of the separation region to a second end of the separation region In certain embodiments, the spacing between adjacent posts is such that a first biological particle can pass between the posts but a second biological particle cannot pass between the posts.
摘要翻译：提供用于分离生物颗粒的方法和装置在某些实施方案中，分离装置用于在流动条件下从第二生物颗粒分离第一生物颗粒，并且包括（a）分离区域，b）可操作地连接到第一生物颗粒的第一入口 c）可操作地连接到分离区域的第一端的第二入口端口，d）沿着从分离区域的第一端部延伸的对角轴线的平面对准的两个或更多个柱体，在某些实施方案中，相邻柱之间的间距使得第一生物颗粒可以在柱之间通过，但是第二生物颗粒不能在柱之间通过。

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