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1. 发明申请

WO2009048533A2 REACTIONS WITHIN MICROFLUIDIC CHANNELS 审中-公开
标题翻译：微流控通道内的反应
公开(公告)号：WO2009048533A2
公开(公告)日：2009-04-16
申请号：PCT/US2008/011457
申请日：2008-10-03
申请人： PRESIDENT AND FELLOWS OF HARVARD COLLEGE , STONE, Howard, A. , RISTENPART, William, D. , WAN, Jiandi
发明人： STONE, Howard, A. , RISTENPART, William, D. , WAN, Jiandi
IPC分类号： B01J19/00 , B01L3/00
CPC分类号： B01J19/0093 , B01J2219/00783 , B01J2219/00824 , B01J2219/00828 , B01J2219/00831 , B01J2219/00833 , B01J2219/00837 , B01J2219/0086 , B01J2219/00889 , B01J2219/00891 , B01J2219/00957 , B01J2219/0097 , B01J2219/00986 , B01L3/502776 , B01L2300/0867 , B01L2400/0487 , G01N1/38 , G01N2001/4072
摘要： The present invention generally relates to microfluidics and, in particular, to microfluidic systems useful for determining reactions. In one aspect, two or more fluids are introduced into a microfluidic channel, and are allowed to come into contact. At or near the interface between the fluids, reactants contained within the fluids may react. By determining the reaction rate between the reactants with respect to position within the microfluidic channel, information about the reaction between the reactants can be obtained. The reaction rate may be determined, for instance, by measuring the rate of reaction at two or more points within the channel. In some cases, e.g., if the reaction rate can be determined optically or visually, the channel may be imaged and the image analyzed to determine the reaction rate. As a non-limiting example of a reaction, the reactants may be an enzyme and a substrate, and by determining reaction rates within the channel, Michaelis-Menten kinetics (or other reaction kinetics) of the enzymatic reaction may be determined. It should be understood, however, that other reactions besides Michaelis-Menten kinetics and/or enzymatic reactions may also be determined, including other catalytic or chemical reactions, and the like. The reaction profiles may be linear or non-linear, e.g., second order, third order, etc.
摘要翻译：本发明一般涉及微流体，并且特别涉及用于确定反应的微流体系统。在一个方面，两种或更多种流体被引入微流体通道中，并且被允许接触。在流体之间的界面处或附近，包含在流体内的反应物可以反应。通过确定反应物之间关于微流体通道内位置的反应速率，可以获得关于反应物之间反应的信息。反应速率可以通过例如测量通道内两个或更多点的反应速率来确定。在一些情况下，例如如果可以光学或视觉确定反应速率，则可以对通道进行成像并分析图像以确定反应速率。作为反应的非限制性实例，反应物可以是酶和底物，并且通过确定通道内的反应速率，可以确定酶促反应的米氏（Michaelis-Menten）动力学（或其他反应动力学）。然而，应该理解的是，除Michaelis-Menten动力学和/或酶促反应之外，还可以确定其他反应，包括其他催化或化学反应等。反应曲线可以是线性的或非线性的，例如二阶，三阶等。

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