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1. 发明申请

WO9906422A3 BASE ANALOGUES 审中-公开
标题翻译：基础模拟
公开(公告)号：WO9906422A3
公开(公告)日：1999-06-03
申请号：PCT/GB9802306
申请日：1998-07-31
申请人： NYCOMED AMERSHAM PLC , SIMMONDS ADRIAN , HAMILTON ALAN , SMITH CLIFFORD , LOAKES DAVID , BROWN DANIEL , HILL FERGAL , KUMAR SHIV , NAMPALLI SATYAM , MCDOUGALL MARK
发明人： SIMMONDS ADRIAN , HAMILTON ALAN , SMITH CLIFFORD , LOAKES DAVID , BROWN DANIEL , HILL FERGAL , KUMAR SHIV , NAMPALLI SATYAM , MCDOUGALL MARK
IPC分类号： G01N33/532 , A61K31/7042 , A61K31/7072 , A61P31/12 , C07H19/00 , C07H21/00 , C12Q1/68 , G01N33/53 , C07H19/06
CPC分类号： G01N33/5308 , C07H21/00
摘要： Nucleoside analogues or base analogues having structure (I), where X = O or NH or S, Y = N or CHR or CR , W = N or NR or CHR or CR or S, n = 1 or 2; each R is independently H or O or alkyl or alkenyl or alkoxy or aryl or a reporter moiety; where necessary (i.e. when Y and/or W is N or CR where R is not O) a double bond is present between Y and W or W and W, and Q is H or a sugar or a sugar analogue or a nucleic acid backbone or backbone analogue.
摘要翻译：具有结构（I）的核苷类似物或碱基类似物，其中X = O或NH或S，Y = N或CHR 6或CR 6，W = N或NR 6或CHR 6或CR < 6>或S，n = 1或2; 每个R 6独立地是H或O或烷基或链烯基或烷氧基或芳基或报道部分; 必要时（即当Y和/或W为N或CR 6而R 6不为O时），Y与W或W与W之间存在双键，Q为H或糖或糖类似物或核酸骨架或骨架类似物。

2. 发明申请

WO2004072238A3 TERMINAL-PHOSPHATE-LABELED NUCLEOTIDES WITH NEW LINKERS 审中-公开
标题翻译：带有新连接器的终端磷酸酯标记的核苷酸
公开(公告)号：WO2004072238A3
公开(公告)日：2006-05-18
申请号：PCT/US2004003284
申请日：2004-02-05
申请人： AMERSHAM BIOSCIENCES CORP , KUMAR SHIV , MCDOUGALL MARK , SOOD ANUP , NELSON JOHN , FULLER CARL , MACKLIN JOHN , MITSIS PAUL
发明人： KUMAR SHIV , MCDOUGALL MARK , SOOD ANUP , NELSON JOHN , FULLER CARL , MACKLIN JOHN , MITSIS PAUL
IPC分类号： C12Q1/68 , C12N20060101 , C12N1/00 , C12N9/00 , C12N9/22 , C12P19/34
CPC分类号： C07H19/10 , C07H19/20 , C07H21/00 , C12P19/34
摘要： The present invention describes methods of using terminal-phosphate-labeled nucleotides in the presence of a manganese salt to enhance their substrate properties towards various enzymes. Particularly described are methods of detecting a nucleic acid in a sample, based on the use of terminal-phosphate-labeled nucleotides as substrates for nucleic acid polymerases, in the presence of a manganese salt. Further provided are manganese complexes of terminal-phosphate-labeled nucleotides as well as terminal-phosphate-labeled nucleotides with new linkers with enhanced substrate properties.
摘要翻译：本发明描述了在锰盐存在下使用末端磷酸酯标记的核苷酸以增强其对各种酶的底物性质的方法。特别描述的是在锰盐存在的情况下，基于使用末端磷酸酯标记的核苷酸作为核酸聚合酶的底物，检测样品中的核酸的方法。还提供了末端磷酸酯标记的核苷酸的锰络合物以及具有增强的底物性质的新接头的末端磷酸酯标记的核苷酸。

3. 发明申请

WO2004085539A3 CYANINE DYE LABELLING REAGENTS WITH MESO-SUBSTITUTION 审中-公开
标题翻译：具有MESO替代品的氰基染料标签试剂
公开(公告)号：WO2004085539A3
公开(公告)日：2006-01-12
申请号：PCT/US2004007833
申请日：2004-03-12
申请人： AMERSHAM BIOSCIENCES CORP , CHEN CHUNG-YUAN , KUMAR SHIV
发明人： CHEN CHUNG-YUAN , KUMAR SHIV
IPC分类号： C07D209/24 , C09B23/01 , C09B23/02 , G01N33/58 , G01N33/533 , C07D209/02 , C07D263/62 , C07D277/60 , C07K17/06 , G01N33/566
CPC分类号： C09B23/164 , C07D209/24 , C09B23/0058 , C09B23/02 , G01N33/582
摘要： Disclosed are cyanine dyes having meso-substituents in the polymethine chain linking the heterocyclic ring systems. The dyes are of formula (I) in which groups R
摘要翻译：公开了在连接杂环体系的聚甲炔链中具有内消旋取代基的花青染料。染料具有式（I），其中R 1〜

4. 发明申请

WO2004072297A3 TERMINAL-PHOSPHATE-LABELED NUCLEOTIDES AND METHODS OF USE 审中-公开
标题翻译：终端磷酸酯标记的核苷酸和使用方法
公开(公告)号：WO2004072297A3
公开(公告)日：2007-01-18
申请号：PCT/US2004002785
申请日：2004-01-30
申请人： AMERSHAM BIOSCIENCES CORP , FULLER CARL , KUMAR SHIV , SOOD ANUP , NELSON JOHN
发明人： FULLER CARL , KUMAR SHIV , SOOD ANUP , NELSON JOHN
IPC分类号： C12Q1/68 , C07H19/10 , C07H19/20 , C07H21/00 , C12Q20060101 , C12Q1/00 , C12Q1/42 , G01N33/52 , G01N33/58
CPC分类号： C12Q1/6823 , C07H19/10 , C07H19/20 , C07H21/00 , C12Q1/6816 , C12Q1/6851 , C12Q2521/525
摘要： The present invention relates to improved methods of detecting a target using a labeled substrate or substrate analog. The methods comprise reacting the substrate or substrate analog in an enzyme-catalyzed reaction which produces a labeled moiety with independently detectable signal only when such substrate or substrate analog reacts. The present invention, in particular, describes methods of detecting a nucleic acid in a sample, based on the use of terminal-phosphate-labeled nucleotides as substrate for nucleic acid polymerases. The methods provided by this invention utilize a nucleoside polyphosphate, dideoxynucleoside polyphosphate, or deoxynucleoside polyphosphate analogue which has a colorimetric dye, chemiluminescent, or fluorescent moiety, a mass tag or an electrochemical tag attached to the terminal-phosphate. When a nucleic acid polymerase uses this analogue as a substrate, an enzyme-activatable label would be present on the inorganic polyphosphate by-product of phosphoryl transfer. Cleavage of the polyphosphate product of phosphoryl transfer via phosphatase leads to a detectable change in the label attached thereon. When the polymerase assay is performed in the presence of a phosphatase, there is provided a convenient method for real-time monitoring of DNA or RNA synthesis and detection of a target nucleic acid.
摘要翻译：本发明涉及使用标记的底物或底物类似物检测靶的改进方法。所述方法包括在酶催化的反应中使底物或底物类似物反应，其仅在这种底物或底物类似物反应时产生具有独立可检测信号的标记部分。本发明特别地描述了基于使用末端磷酸酯标记的核苷酸作为核酸聚合酶的底物来检测样品中核酸的方法。本发明提供的方法利用了具有比色染料，化学发光或荧光部分的核苷多磷酸，双脱氧核苷多聚磷酸酯或脱氧核苷多聚磷酸酯类似物，连接至末端磷酸酯的质量标签或电化学标签。当核酸聚合酶使用该类似物作为底物时，酶活性标记将存在于磷酸转移的无机多磷酸盐副产物上。通过磷酸酶切割磷酸转移的多磷酸盐产物导致附着在其上的标记物的可检测的变化。当在磷酸酶的存在下进行聚合酶测定时，提供了用于实时监测DNA或RNA合成和检测靶核酸的方便的方法。

5. 发明申请

WO2004029579A3 FLUORESCENT LABELING REAGENTS WITH MULTIPLE DONORS AND ACCEPTORS 审中-公开
标题翻译：荧光标签试剂与多个捐助者和接受者
公开(公告)号：WO2004029579A3
公开(公告)日：2004-08-19
申请号：PCT/US0330361
申请日：2003-09-25
申请人： AMERSHAM BIOSCIENCES CORP , KUMAR SHIV , CHEN CHUNG-YUAN
发明人： KUMAR SHIV , CHEN CHUNG-YUAN
IPC分类号： C07D311/82 , C07D405/14 , C07D473/02 , C07D487/04 , C07D491/22 , C09B11/04 , C09B11/08 , C09B11/24 , C12Q1/68 , C07D311/80
CPC分类号： C09B11/08 , C07D311/82 , C07D405/14 , C07D487/04 , C09B11/04 , C09B11/24 , C12Q1/6869 , C12Q2527/125
摘要： Fluorescent labeling reagents with multiple donors and acceptors, based on and synthesized from easily prepared dye building blocks.
摘要翻译：荧光标记试剂与多个供体和受体，基于并由易于制备的染料积木合成。

6. 发明申请

WO2004029578A3 ENERGY TRANSFER DYES, TERMINATORS AND USE THEREOF 审中-公开
标题翻译：能量转移染料，终止剂及其用途
公开(公告)号：WO2004029578A3
公开(公告)日：2004-07-08
申请号：PCT/US0330360
申请日：2003-09-25
申请人： AMERSHAM BIOSCIENCES CORP , KUMAR SHIV , CHEN CHUNG-YUAN , RAO SUDHAKAR
发明人： KUMAR SHIV , CHEN CHUNG-YUAN , RAO SUDHAKAR
IPC分类号： C07D311/82 , C07D405/14 , C07D473/02 , C07D487/04 , C07D491/22 , C09B11/04 , C09B11/08 , C09B11/24 , C12Q1/68 , C07H21/04 , C12P19/34
CPC分类号： C09B11/08 , C07D311/82 , C07D405/14 , C07D487/04 , C09B11/04 , C09B11/24 , C12Q1/6869 , C12Q2527/125
摘要： The present invention relates to a novel set of four fluorescently labeled dye terminators. Two of them are single dye labeled terminators and the other two dye terminators are based on fluorescent resonance energy transfer (FRET). The FRET dye terminators are generated from the 4', 5'-bis-aminomethylfluorescein. Of the two amino groups of the donor dye, 4', 5'-bis-aminomethylfluorescein, one amino group is used to attach the acceptor dye, and the other amino group is used to attach the dideoxynucleoside-5'-triphosphate. Their preparation, energy transfer efficiency, and use as labels, specifically, in DNA sequencing reactions is disclosed.
摘要翻译：本发明涉及一组新的四种荧光标记的染料终止剂。其中两个是单染料标记的终止子，另两个染料终止子基于荧光共振能量转移（FRET）。 FRET染料终止子由4'，5'-双 - 氨基甲基荧光素产生。在供体染料的两个氨基中，4'，5'-双氨基甲基荧光素，一个氨基用于连接受体染料，另一个氨基用于连接双脱氧核苷-5'-三磷酸。它们的制备，能量转移效率和作为标记的用途，特别是在DNA测序反应中被公开。

7. 发明申请

WO2013154999A3 METHOD OF PREPARATION OF NANOPORE AND USES THEREOF 审中-公开
标题翻译：制备纳米材料的方法及其用途
公开(公告)号：WO2013154999A3
公开(公告)日：2014-11-20
申请号：PCT/US2013035635
申请日：2013-04-08
申请人： UNIV COLUMBIA , US GOVERNMENT , JU JINGYUE , KUMAR SHIV , TAO CHUANJUAN , CHIEN MINCHEN , RUSSO JAMES J , KASIANOWICZ JOHN J , ROBERTSON JOSEPH W F
发明人： JU JINGYUE , KUMAR SHIV , TAO CHUANJUAN , CHIEN MINCHEN , RUSSO JAMES J , KASIANOWICZ JOHN J , ROBERTSON JOSEPH W F
IPC分类号： C12Q1/42
CPC分类号： C12Q1/6883 , B82Y5/00 , C07H19/06 , C07H19/16 , C12Q1/42 , C12Q1/48 , C12Q1/6869 , C12Q2600/158 , G01N33/48721 , G01N2333/9125 , C12Q2565/631 , C12Q2521/525 , C12Q2521/543 , C12Q2535/107
摘要： This disclosure provides systems and methods for sequencing nucleic acids using nucleotide analogues and translocation of tags from incorporated nucleotide analogues through a nanopore. In aspects, this disclosure is related to composition, method, and system for sequencing a nucleic acid using tag molecules and detection of translocation through a nanopore of tags released from incorporation of the molecule.
摘要翻译：本公开提供了使用核苷酸类似物对核酸进行测序的系统和方法，以及通过纳米孔从掺入的核苷酸类似物转移标签。在一些方面，本公开涉及使用标签分子对核酸测序的组合物，方法和系统，以及通过从分子掺入释放的标记的纳米孔的易位检测。

8. 发明申请

WO2004071155A2 SOLID PHASE SEQUENCING 审中-公开
标题翻译：固相序列
公开(公告)号：WO2004071155A2
公开(公告)日：2004-08-26
申请号：PCT/US2004003283
申请日：2004-02-05
申请人： AMERSHAM BIOSCIENCES CORP , SOOD ANUP , KUMAR SHIV , NELSON JOHN , FULLER CARL
发明人： SOOD ANUP , KUMAR SHIV , NELSON JOHN , FULLER CARL
IPC分类号： C12P19/34 , C12Q1/68 , G01R29/18
CPC分类号： C12Q1/6858 , C12Q1/6874 , C12Q2565/537 , C12Q2535/125 , C12Q2521/525 , C12Q2525/101
摘要： The present invention describes methods of sequencing a nucleic acid in a sample, based on the use of terminal-phosphate-labeled nucleotides as substrates for nucleic acid polymerases. The methods provided by this invention utilize a nucleoside polyphosphate, dideoxynucleoside polyphosphate, or deoxynucleoside polyphosphate analogue which has a colorimetric dye, chemiluminescent, or fluorescent moiety, a mass tag or an electrochemical tag attached to the terminal-phosphate. When a nucleic acid polymerase uses this analogue as a substrate, an enzyme-activatable label would be present on the inorganic polyphosphate by-product of phosphoryl transfer. Cleavage of the polyphosphate product of phosphoryl transfer via phosphatase leads to a detectable change in the label attached thereon. In some instances the labeled polyphosphate may be detected directly via the label and provide information on the nucleic acid. When the polymerase assay is performed in the presence of a phosphatase, there is provided a convenient method for real-time monitoring of DNA or RNA synthesis and characterization of a target nucleic acid.
摘要翻译：本发明描述了基于使用末端磷酸酯标记的核苷酸作为核酸聚合酶的底物来测序样品中核酸的方法。本发明提供的方法利用具有比色染料，化学发光或荧光部分的核苷多磷酸，双脱氧核苷多磷酸酯或脱氧核苷多聚磷酸酯类似物，连接至末端磷酸酯的质量标签或电化学标签。当核酸聚合酶使用该类似物作为底物时，酶活性标记将存在于磷酸转移的无机多磷酸盐副产物上。通过磷酸酶切割磷酸转移的多磷酸盐产物导致附着在其上的标记物的可检测的变化。在一些情况下，标记的多磷酸盐可以通过标记物直接检测并提供关于核酸的信息。当在磷酸酶存在下进行聚合酶测定时，提供了用于实时监测DNA或RNA合成和靶核酸表征的方便的方法。

9. 发明申请

WO2015123430A2 SINGLE MOLECULE ELECTRONIC MULTIPLEX SNP ASSAY AND PCR ANALYSIS 审中-公开
标题翻译：单分子电子多重SNP测定和PCR分析
公开(公告)号：WO2015123430A2
公开(公告)日：2015-08-20
申请号：PCT/US2015015647
申请日：2015-02-12
申请人： UNIV COLUMBIA , TAO CHUANJUAN , KUMAR SHIV , CHIEN MINCHEN , JU JINGYUE
发明人： TAO CHUANJUAN , KUMAR SHIV , CHIEN MINCHEN , JU JINGYUE
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6858 , C12Q1/6853 , C12Q2525/186 , C12Q2565/631 , C12Q2535/113
摘要： This invention provides methods of using labeled primers or probes for nucleic acid target detection and to detect the identity or presence of a nucleotide at certain positions in nucleic acid sequences with single molecule sensitivity using nanopore detection, and sets of oligonucleotide primers for use in such methods, as well as methods of quantitative PCR coupled with nanopore detection.
摘要翻译：本发明提供使用标记的引物或探针用于核酸靶标检测的方法，并使用纳米孔检测法检测具有单分子敏感性的核酸序列的某些位置的核苷酸的同一性或存在，以及用于这些方法的寡核苷酸引物组，以及与纳米孔检测结合的定量PCR方法。

10. 发明申请

WO2012083249A2 DNA SEQUENCING BY SYNTHESIS USING MODIFIED NUCLEOTIDES AND NANOPORE DETECTION 审中-公开
标题翻译：通过使用修饰的核苷酸和纳米RNA检测合成DNA
公开(公告)号：WO2012083249A2
公开(公告)日：2012-06-21
申请号：PCT/US2011065640
申请日：2011-12-16
申请人： UNIV COLUMBIA , JU JINGYUE , KUMAR SHIV , LI ZENGMIN , TAO CHUANJUAN , CHIEN MINCHEN , RUSSO JAMES J , KALACHIKOV SERGEY , SHEPARD KEN , ROSENSTEIN JACOB KARL
发明人： JU JINGYUE , KUMAR SHIV , LI ZENGMIN , TAO CHUANJUAN , CHIEN MINCHEN , RUSSO JAMES J , KALACHIKOV SERGEY , SHEPARD KEN , ROSENSTEIN JACOB KARL
CPC分类号： C12Q1/6869 , C07H19/10 , C07H21/00 , G01N33/48721 , C12Q2525/113 , C12Q2525/117 , C12Q2565/518 , C12Q2565/631
摘要： This disclosure is related to a method of sequencing a single- stranded DNA using deoxynucleotide polyphosphate analogues and translocation of tags from incorporated deoxynucleotide polyphosphate analogues through a nanopore.
摘要翻译：本公开涉及使用脱氧核苷酸多磷酸酯类似物测序单链DNA的方法和通过纳米孔从掺入的脱氧核苷酸多磷酸酯类似物转移标签的方法。

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