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1. 发明申请

WO2004005933A1 REAGENTS AND A METHOD FOR SATURATION LABELLING OF PROTEINS 审中-公开
标题翻译：试剂和蛋白质饱和标记方法
公开(公告)号：WO2004005933A1
公开(公告)日：2004-01-15
申请号：PCT/GB2002/003142
申请日：2002-07-08
申请人： AMERSHAM BIOSCIENCES UK LIMITED , WILLIAMS, Karen , STONE, Timothy , SIMMONDS, Adrian, Christopher , SWEET, Alison, Claire , FOWLER, Susan, Janet
发明人： WILLIAMS, Karen , STONE, Timothy , SIMMONDS, Adrian, Christopher , SWEET, Alison, Claire , FOWLER, Susan, Janet
IPC分类号： G01N33/68
CPC分类号： C07K1/13 , C09B23/02 , C09B67/0034 , G01N33/6815
摘要： A matched set of fluorescent dyes is provided, wherein each dye of the set is capable ofcovalent attachment to a protein and wherein each of the dyes has a molecular structure and a charge that is matched one with the other, such that relative electrophoretic mobility of a protein labelled with one dye of the set is the same as the electrophoretic mobility of the protein labelled with a different dye of the set. The matched set comprises at least two different fluorescent dyes of formula:wherein n is 1, 2, or 3; Z1 and Z2 independently represent the carbon atoms necessary to complete a phenyl or naphthyl ring system; one of groups R1 and R2 is a target bonding group; remaining group R1 or R2 is selected from -(CH2)4-W or -(CH2)r-H; group R3 is hydrogen, except when either R1 or R2 is -(CH2)r-H, in which case R3 is W; and W is selected from sulphonic acid and sulphonate. The invention also provides a method for saturation labelling of a protein with a fluorescent dye so as to label all available target amino acid, suitably cysteine, residues in the protein, thereby giving a single population of labelled protein molecules.
摘要翻译：提供了一组匹配的荧光染料，其中该组的每种染料能够与蛋白质共价连接，并且其中每种染料具有与另一种相匹配的分子结构和电荷，使得相对电泳迁移率用该组中的一种染料标记的蛋白质与用该组不同染料标记的蛋白质的电泳迁移率相同。匹配组包含至少两种下式的不同荧光染料：其中n为1,2或3; Z 1和Z 2独立地表示完成苯基或萘基环系所需的碳原子; R 1和R 2中的一个是目标键合基团; 剩余的基团R1或R2选自 - （CH2）4-W或 - （CH2）r-H; R 3是氢，除了当R 1或R 2是 - （CH 2）r-H时，其中R 3是W; W选自磺酸和磺酸盐。本发明还提供了用荧光染料饱和标记蛋白质的方法，以便标记蛋白质中的所有可用的靶氨基酸，适当的半胱氨酸残基，由此得到单个标记的蛋白质分子群。

2. 发明申请

WO2003048178A3 NUCLEOTIDE ANALOGUES AND THEIR USE FOR NUCLEIC ACID SEQUENCING 审中-公开
公开(公告)号：WO2003048178A3
公开(公告)日：2003-06-12
申请号：PCT/GB2002/005375
申请日：2002-11-28
申请人： AMERSHAM BIOSCIENCES UK LIMITED , PICKERING, Lea , ODEDRA, Raj , SIMMONDS, Adrian , JOHNSON, Karin, Sofia, Helena
发明人： PICKERING, Lea , ODEDRA, Raj , SIMMONDS, Adrian , JOHNSON, Karin, Sofia, Helena
IPC分类号： C07H19/00
摘要： The invention relates to nucleosides comprising a reporter moiety which also functions to limit polymerase activity, characterised in that the reporter moiety is attached to the nucleoside through a linkage group cleavable by a hydrolase enzyme wherein the hydrolase enzyme is selected from the group consisting of esterases, phosphatases, peptidases, penicillin amidases, glycosidases and phosphorylases.

3. 发明申请

WO1997029154A1 BENZOPHENOXAZINE DYES 审中-公开
标题翻译：苯并噻唑染料
公开(公告)号：WO1997029154A1
公开(公告)日：1997-08-14
申请号：PCT/GB1997000324
申请日：1997-02-05
申请人： AMERSHAM INTERNATIONAL PLC , SIMMONDS, Adrian , MILLER, James, N. , MOODY, Christopher, John , SWANN, Elizabeth , BRIGGS, Mark, Samuel, Jonathan , BRUCE, Ian, Edward
发明人： AMERSHAM INTERNATIONAL PLC
IPC分类号： C09B19/00
CPC分类号： C07D413/12 , C07D265/34 , C09B19/00 , G01N33/533
摘要： Benzophenoxazine compounds have formula (I) where X is O or NH, Y is NR R or H, R and R are alkyl or -L-A, L is a linker and A may be a reactive group by means of which the compound is linked to a biomolecule. The compounds can be used as fluorescent dyes for labelling biomolecules.
摘要翻译：苯并恶嗪化合物具有式（I），其中X是O或NH，Y是NR 1 R 2或H，R 1和R 2是烷基或-LA，L是连接基，A可以是化合物与生物分子连接的反应性基团。该化合物可用作标记生物分子的荧光染料。

4. 发明申请

WO2003048178A2 NUCLEOTIDE ANALOGUES 审中-公开
标题翻译：核苷酸类似物
公开(公告)号：WO2003048178A2
公开(公告)日：2003-06-12
申请号：PCT/GB2002/005375
申请日：2002-11-28
申请人： AMERSHAM BIOSCIENCES UK LIMITED , JOHNSON, Karin, Sofia, Helena , PICKERING, Lea , ODEDRA, Raj , SIMMONDS, Adrian
发明人： PICKERING, Lea , ODEDRA, Raj , SIMMONDS, Adrian
IPC分类号： C07H19/00
CPC分类号： C07H19/06 , C07H19/10 , C09B11/08 , C12Q1/6869 , C12Q2525/117
摘要： The invention relates to nucleosides comprising a reporter moiety which also functions to limit polymerase activity, characterised in that the reporter moiety is attached to the nucleoside through a linkage group cleavable by a hydrolase enzyme wherein the hydrolase enzyme is selected from the group consisting of esterases, phosphatases, peptidases, penicillin amidases, glycosidases and phosphorylases.
摘要翻译：本发明涉及包含报道部分的核苷，其也起到限制聚合酶活性的作用，其特征在于报道部分通过可由水解酶切割的连接基团与核苷连接，其中水解酶选自酯酶，磷酸酶，肽酶，青霉素酰胺酶，糖苷酶和磷酸酶。

5. 发明申请

WO1997028176A1 NUCLEOSIDE ANALOGUES 审中-公开
标题翻译：核苷类似物
公开(公告)号：WO1997028176A1
公开(公告)日：1997-08-07
申请号：PCT/GB1997000306
申请日：1997-02-03
申请人： AMERSHAM INTERNATIONAL PLC , BROWN, Daniel , HAMILTON, Alan , LOAKES, David , SIMMONDS, Adrian , SMITH, Clifford
发明人： AMERSHAM INTERNATIONAL PLC
IPC分类号： C07H19/044
CPC分类号： C07H21/00 , C07H19/044 , C07H19/052 , C07H19/056
摘要： Nucleoside analogues in which a group M replaces the natural base where M is (1) or (2) or (3), where each of X , X and X are C or N, each of R and R is the same or different and each is H, NO2, CO, COR , OR , CN, O, CON(R )2, COOR , SO2R , SO3R , SR , NHCHO, (CH2)n(R )2, halogen, or a reporter moiety, each of R and R is H or hydrocarbyl or a reporter moiety, and n is 0-4. The analogues are substrates for polymerase and terminal transferase enzymes.
摘要翻译：其中M代替M为（1）或（2）或（3）的天然碱基的核苷类似物，其中X 1，X 2和X 3各自为C或N， R 6和R 7相同或不同，分别为H，NO 2，CO，COR 8，OR 8，CN，O，CON（R 8）2，COOR 8 ，SO 2 R 8，SO 3 R 8，SR 8，NHCHO，（CH 2）n（R 8）2，卤素或报道分子部分，R 8和R 9各自为H 或烃基或报道部分，n为0-4。类似物是聚合酶和末端转移酶的底物。

6. 发明申请

WO9906422A3 BASE ANALOGUES 审中-公开
标题翻译：基础模拟
公开(公告)号：WO9906422A3
公开(公告)日：1999-06-03
申请号：PCT/GB9802306
申请日：1998-07-31
申请人： NYCOMED AMERSHAM PLC , SIMMONDS ADRIAN , HAMILTON ALAN , SMITH CLIFFORD , LOAKES DAVID , BROWN DANIEL , HILL FERGAL , KUMAR SHIV , NAMPALLI SATYAM , MCDOUGALL MARK
发明人： SIMMONDS ADRIAN , HAMILTON ALAN , SMITH CLIFFORD , LOAKES DAVID , BROWN DANIEL , HILL FERGAL , KUMAR SHIV , NAMPALLI SATYAM , MCDOUGALL MARK
IPC分类号： G01N33/532 , A61K31/7042 , A61K31/7072 , A61P31/12 , C07H19/00 , C07H21/00 , C12Q1/68 , G01N33/53 , C07H19/06
CPC分类号： G01N33/5308 , C07H21/00
摘要： Nucleoside analogues or base analogues having structure (I), where X = O or NH or S, Y = N or CHR or CR , W = N or NR or CHR or CR or S, n = 1 or 2; each R is independently H or O or alkyl or alkenyl or alkoxy or aryl or a reporter moiety; where necessary (i.e. when Y and/or W is N or CR where R is not O) a double bond is present between Y and W or W and W, and Q is H or a sugar or a sugar analogue or a nucleic acid backbone or backbone analogue.
摘要翻译：具有结构（I）的核苷类似物或碱基类似物，其中X = O或NH或S，Y = N或CHR 6或CR 6，W = N或NR 6或CHR 6或CR < 6>或S，n = 1或2; 每个R 6独立地是H或O或烷基或链烯基或烷氧基或芳基或报道部分; 必要时（即当Y和/或W为N或CR 6而R 6不为O时），Y与W或W与W之间存在双键，Q为H或糖或糖类似物或核酸骨架或骨架类似物。

7. 发明申请

WO1997028177A1 NUCLEOSIDE ANALOGUES 审中-公开
标题翻译：核苷类似物
公开(公告)号：WO1997028177A1
公开(公告)日：1997-08-07
申请号：PCT/GB1997000312
申请日：1997-02-03
申请人： AMERSHAM INTERNATIONAL PLC , BROWN, Daniel , HAMILTON, Alan , LOAKES, David , SIMMONDS, Adrian , SMITH, Clifford
发明人： AMERSHAM INTERNATIONAL PLC
IPC分类号： C07H19/06
CPC分类号： C07H19/06 , C07H21/00 , C12Q1/686 , C12Q1/6869 , G01N33/5308
摘要： Nucleoside analogues containing the degenerate base analogue P and derivatives thereof are provided with reporter moieties preferably comprising signal moieties. The nucleoside analogues are useful for labelling DNA or RNA or for incorporating in oligonucleotides.
摘要翻译：含有简并碱基类似物P及其衍生物的核苷类似物提供有优选包含信号部分的报道部分。核苷类似物可用于标记DNA或RNA或用于掺入寡核苷酸。

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