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1. 发明授权

US06677140B2 Nucleic acid amplification with DNA-dependent RNA polymerase activity of RNA replicases 失效
标题翻译：核酸扩增与RNA复制酶的DNA依赖性RNA聚合酶活性
公开(公告)号：US06677140B2
公开(公告)日：2004-01-13
申请号：US10071057
申请日：2002-02-07
申请人： Randall L. Dimond , Steven J. Ekenberg , James R. Hartnett , Geoffrey R. Hudson , Leopoldo G. Mendoza , Katharine M. Miller , John E. Monahan , Christopher L. Jones , Mark A. Maffitt , Richard A. Martinelli , Edward E. Pahuski , James W. Schumm
发明人： Randall L. Dimond , Steven J. Ekenberg , James R. Hartnett , Geoffrey R. Hudson , Leopoldo G. Mendoza , Katharine M. Miller , John E. Monahan , Christopher L. Jones , Mark A. Maffitt , Richard A. Martinelli , Edward E. Pahuski , James W. Schumm
IPC分类号： C12P1934
CPC分类号： C12Q1/6867 , C12Q1/6806 , C12Q1/6853 , C12Q2521/501 , C12Q2521/325 , C12Q2521/119 , C12Q2521/107 , C12Q2563/137 , C12Q2531/149 , C12Q2525/121
摘要： The present invention entails methods and kits for carrying them out based on the discovery that an RNA replicase, such as Q&bgr; replicase, has DNA-dependent RNA polymerase (“DDRP”) activity with nucleic acid segments, including DNA segments and DNA:RNA chimeric segments, which comprise a 2′-deoxyribonucleotide or an analog thereof and which have sequences of RNAs that are autocatalytically replicatable by the replicase.
摘要翻译：本发明涉及用于携带RNA的复制酶如Qbeta复制酶具有核酸片段的DNA依赖性RNA聚合酶（“DDRP”）活性的发现的方法和试剂盒，包括DNA片段和DNA：RNA嵌合其包含2'-脱氧核糖核苷酸或其类似物，并且具有由复制酶自动催化复制的RNA序列。

2. 发明申请

US20110172111A1 SOLID PHASE SEQUENCING OF BIOPOLYMERS 失效
标题翻译：生物聚合物的固相序列
公开(公告)号：US20110172111A1
公开(公告)日：2011-07-14
申请号：US12852336
申请日：2010-08-06
申请人： Charles Cantor , Hubert Köster
发明人： Charles Cantor , Hubert Köster
IPC分类号： C40B30/04
CPC分类号： C12Q1/6872 , C12Q1/6874 , C12Q2565/501 , C12Q2531/113 , C12Q2521/525 , C12Q2565/627 , C12Q2531/119 , C12Q2523/301 , C12Q2531/137 , C12Q2521/301 , C12Q2531/149
摘要： This invention relates to methods for detecting and sequencing target nucleic acid sequences, to mass modified nucleic acid probes and arrays of probes useful in these methods, and to kits and systems which contain these probes. Useful methods involve hybridizing the nucleic acids or nucleic acids which represent complementary or homologous sequences of the target to an array of nucleic acid probes. These probes comprise a single-stranded portion, an optional double-stranded portion and a variable sequence within the single-stranded portion. The molecular weights of the hybridized nucleic acids of the set can be determined by mass spectroscopy, and the sequence of the target determined from the molecular weights of the fragments. Nucleic acids whose sequences can be determined include DNA or RNA in biological samples such as patient biopsies and environmental samples. Probes may be fixed to a solid support such as a hybridization chip to facilitate automated molecular weight analysis and identification of the target sequence.
摘要翻译：本发明涉及用于检测和测序靶核酸序列，批量修饰的核酸探针和可用于这些方法的探针阵列的方法，以及含有这些探针的试剂盒和系统。有用的方法包括将代表靶的互补或同源序列的核酸或核酸与核酸探针阵列进行杂交。这些探针包括在单链部分内的单链部分，任选的双链部分和可变序列。该组的杂交核酸的分子量可以通过质谱法测定，并且靶标的序列由片段的分子量确定。可以确定其序列的核酸包括生物样品中的DNA或RNA，例如患者活组织检查和环境样品。探针可以固定在固体支持物例如杂交芯片上以促进自动化分子量分析和靶序列的鉴定。

3. 发明申请

US20100285463A1 SELECTING NUCLEIC ACID SAMPLES SUITABLE FOR GENOTYPING 审中-公开
标题翻译：选择适合基因的核酸样品
公开(公告)号：US20100285463A1
公开(公告)日：2010-11-11
申请号：US12667135
申请日：2008-06-27
申请人： Florian Kronenberg , Veit Schoenborn , Iris Heid , Anita Brandstätter
发明人： Florian Kronenberg , Veit Schoenborn , Iris Heid , Anita Brandstätter
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6827 , C12Q2531/149 , C12Q2525/179
摘要： The present invention relates to a method for selecting a DNA sample comprising genomic DNA suitable for genotyping, comprising the steps of: i) pre-genotyping said genomic DNA using a set of polymorphic markers; ii) determining out of said set of polymorphic markers the percentage of polymorphic markers for which said genomic DNA is homozygous; and iii) selecting said DNA sample when said genomic DNA is homozygous for less than 70% of said set of polymorphic markers. Furthermore, a method of genotyping comprising a step of using a DNA sample selected by the method in accordance with the present invention and/or a step of applying the method provided herein is disclosed. The present invention also relates to a method for identifying a gene or a locus on a genome, said method comprising a step of using a DNA sample selected by the method provided herein and a step of applying the method described herein. Further, the present invention relates to a kit for carrying out the method in accordance with the present invention comprising primers for the amplification of the set of polymorphic markers employed herein.
摘要翻译：本发明涉及一种用于选择包含适合基因分型的基因组DNA的DNA样品的方法，其包括以下步骤：i）使用一组多态性标记对所述基因组DNA进行前基因分型; ii）确定所述多态性标记集合中所述基因组DNA是纯合的多态标志物的百分比; 以及iii）当所述基因组DNA对所述多态性标记物组的少于70％是纯合的时，选择所述DNA样品。此外，公开了一种基因分型方法，其包括使用根据本发明的方法选择的DNA样品和/或应用本文提供的方法的步骤。本发明还涉及用于鉴定基因组上的基因或基因座的方法，所述方法包括使用通过本文提供的方法选择的DNA样品的步骤和应用本文所述方法的步骤。此外，本发明涉及用于实施根据本发明的方法的试剂盒，其包含用于扩增本文使用的多态性标记组的引物。

4. 发明申请

US20030180725A1 Compositions, methods, kits and apparatus for determining the presence or absence of target molecules 失效
标题翻译：用于确定靶分子存在或不存在的组合物，方法，试剂盒和装置
公开(公告)号：US20030180725A1
公开(公告)日：2003-09-25
申请号：US09844935
申请日：2001-04-27
申请人： Q-RNA, INC.
发明人： Alexander Munishkin , Abraham Grossman
IPC分类号： C12Q001/68 , C07H021/02 , C12P019/34
CPC分类号： C12Q1/6811 , C12Q2531/149
摘要： The present invention is directed to methods, compositions, kits and apparatus to identify and detect the presence or absence of target analytes. The embodiments of the present invention have utility in medical diagnosis and analysis of various chemical compounds in specimens and samples, as well as the design of test kits and apparatus for implementing such methods.
摘要翻译：本发明涉及用于鉴定和检测目标分析物的存在或不存在的方法，组合物，试剂盒和装置。本发明的实施例在标本和样品中的各种化学化合物的医学诊断和分析中具有实用性，以及用于实施这些方法的测试试剂盒和设备的设计。

5. 发明授权

US06562575B1 Analyte-specific assays based on formation of a replicase substrate 有权
标题翻译：基于复制酶底物形成的分析物特异性测定
公开(公告)号：US06562575B1
公开(公告)日：2003-05-13
申请号：US09891661
申请日：2001-06-26
申请人： Gary A. Dahl
发明人： Gary A. Dahl
IPC分类号： C12Q168
CPC分类号： C12Q1/682 , C12Q2531/149
摘要： Methods and compositions are provided for assaying for a target analyte in a sample by formation of a substrate for a replicase. The target analyte, if present in the sample, is first bound to a reporter probe. The reporter probe comprises a first portion and a second portion. The first portion comprises a polynucleotide that encodes at least part of a sequence for an RNA that is a substrate for replication. The second portion comprises a molecule that has affinity for an analyte. The reporter probe itself is not a substrate for a replicase. However, a replicase substrate is generated by treating reporter probe which is bound to analyte with a composition having nuclease activity in order to release the parts of the first portion, and then the released parts and one or more mononucleotides or oligonucleotides comprising missing parts of the substrate sequence are joined with a composition having ligase activity. Newly formed substrate is replicated with a replicase, and a signal is measured which indicates the presence and quantity of analyte in the sample. The invention also provides methods and compositions for simultaneously assaying for any of multiple analytes in a sample.
摘要翻译：提供方法和组合物用于通过形成用于复制酶的底物来测定样品中的目标分析物。目标分析物（如果存在于样品中）首先与报道探针结合。记录探针包括第一部分和第二部分。第一部分包含编码作为复制底物的RNA的序列的至少一部分的多核苷酸。第二部分包含对分析物具有亲和性的分子。报道探针本身不是复制酶的底物。然而，通过用与具有核酸酶活性的组合物结合分析物的报道探针来释放复制酶底物以释放第一部分的部分，然后释放的部分和一个或多个单核苷酸或寡核苷酸包含缺失的部分底物序列与具有连接酶活性的组合物连接。用复制酶复制新形成的底物，并且测量指示样品中分析物的存在和量的信号。本发明还提供用于同时测定样品中多种分析物中的任何一种的方法和组合物。

6. 发明授权

US06225058B1 Compositions, methods, kits and apparatus for determining the presence or absence of target molecules 失效
标题翻译：用于确定靶分子存在或不存在的组合物，方法，试剂盒和装置
公开(公告)号：US06225058B1
公开(公告)日：2001-05-01
申请号：US09229287
申请日：1999-01-13
申请人： Alexander Munishkin , Abraham Grossman
发明人： Alexander Munishkin , Abraham Grossman
IPC分类号： C12Q168
CPC分类号： C12Q1/6811 , C12Q2531/149
摘要： The present invention is directed to methods, compositions, kits and apparatus to identify and detect the presence or absence of target analytes. The embodiments of the present invention have utility in medical diagnosis and analysis of various chemical compounds in specimens and samples, as well as the design of test kits and apparatus for implementing such methods.
摘要翻译：本发明涉及用于鉴定和检测目标分析物的存在或不存在的方法，组合物，试剂盒和装置。本发明的实施例在标本和样品中的各种化学化合物的医学诊断和分析中具有实用性，以及用于实施这些方法的测试试剂盒和设备的设计。

7. 发明授权

US06207388B1 Compositions, methods, kits and apparatus for determining the presence or absence of target molecules 失效
公开(公告)号：US06207388B1
公开(公告)日：2001-03-27
申请号：US09437034
申请日：1999-11-09
申请人： Abraham Grossman
发明人： Abraham Grossman
IPC分类号： C12Q168
CPC分类号： C12Q1/6862 , C12Q1/6867 , C12Q2531/149 , C12Q2531/137
摘要： The present invention is directed to methods, compositions, kits and apparatus to identify and detect the presence or absence of target analytes. The embodiments of the present invention have utility in medical diagnosis and analysis of various chemical compounds in specimens and samples, as well as the design of test kits and apparatus for implementing such methods.

8. 发明授权

US06165714A Devices and methods for detecting nucleic acid analytes in samples 失效
标题翻译：用于检测样品中核酸分析物的装置和方法
公开(公告)号：US06165714A
公开(公告)日：2000-12-26
申请号：US991675
申请日：1997-12-16
申请人： David J. Lane , Michael P. Farrell
发明人： David J. Lane , Michael P. Farrell
IPC分类号： G01N33/53 , C12M1/00 , C12N15/09 , C12Q1/68 , G01N37/00 , C12P19/34
CPC分类号： C12Q1/682 , C12Q1/6837 , C12Q2565/537 , C12Q2561/125 , C12Q2531/149
摘要： The invention provides devices and methods for use in detecting nucleic acid analytes in samples. The devices each include a solid support to which is bound a two-dimensional distribution or field of nucleic acid probes that each bind to a nucleic acid analyte, which is detected by use of amplification methods.
摘要翻译：本发明提供了用于检测样品中核酸分析物的装置和方法。这些装置各自包括固体支持物，其与结合核酸分析物的核酸探针的二维分布或场结合，其通过使用扩增方法检测。

9. 发明授权

US08758995B2 Solid phase sequencing of biopolymers 失效
标题翻译：生物聚合物的固相测序
公开(公告)号：US08758995B2
公开(公告)日：2014-06-24
申请号：US12852336
申请日：2010-08-06
申请人： Charles R. Cantor , Hubert Koster
发明人： Charles R. Cantor , Hubert Koster
IPC分类号： C12Q1/68 , C12P19/34 , C07H21/02 , C07H21/04
CPC分类号： C12Q1/6872 , C12Q1/6874 , C12Q2565/501 , C12Q2531/113 , C12Q2521/525 , C12Q2565/627 , C12Q2531/119 , C12Q2523/301 , C12Q2531/137 , C12Q2521/301 , C12Q2531/149
摘要： This invention relates to methods for detecting and sequencing target nucleic acid sequences, to mass modified nucleic acid probes and arrays of probes useful in these methods, and to kits and systems which contain these probes. Useful methods involve hybridizing the nucleic acids or nucleic acids which represent complementary or homologous sequences of the target to an array of nucleic acid probes. These probes comprise a single-stranded portion, an optional double-stranded portion and a variable sequence within the single-stranded portion. The molecular weights of the hybridized nucleic acids of the set can be determined by mass spectroscopy, and the sequence of the target determined from the molecular weights of the fragments. Probes may be affixed to a solid support such as a hybridization chip to facilitate automated molecular weight analysis and identification of the target sequence.
摘要翻译：本发明涉及用于检测和测序靶核酸序列，批量修饰的核酸探针和可用于这些方法的探针阵列的方法，以及含有这些探针的试剂盒和系统。有用的方法包括将代表靶的互补或同源序列的核酸或核酸与核酸探针阵列进行杂交。这些探针包括在单链部分内的单链部分，任选的双链部分和可变序列。该组的杂交核酸的分子量可以通过质谱法测定，并且靶标的序列由片段的分子量确定。探针可以固定在诸如杂交芯片的固体支持物上，以便于自动化分子量分析和目标序列的鉴定。

10. 发明授权

US07803529B1 Solid phase sequencing of biopolymers 有权
标题翻译：生物聚合物的固相测序
公开(公告)号：US07803529B1
公开(公告)日：2010-09-28
申请号：US09395409
申请日：1999-09-14
申请人： Charles Cantor , Hubert Köster
发明人： Charles Cantor , Hubert Köster
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6872 , C12Q1/6874 , C12Q2565/501 , C12Q2531/113 , C12Q2521/525 , C12Q2565/627 , C12Q2531/119 , C12Q2523/301 , C12Q2531/137 , C12Q2521/301 , C12Q2531/149
摘要： This invention relates to methods for detecting and sequencing target nucleic acid sequences, to mass modified nucleic acid probes and arrays of probes useful in these methods, and to kits and systems which contain these probes. Useful methods involve hybridizing the nucleic acids or nucleic acids which represent complementary or homologous sequences of the target to an array of nucleic acid probes. These probes comprise a single-stranded portion, an optional double-stranded portion and a variable sequence within the single-stranded portion. The molecular weights of the hybridized nucleic acids of the set can be determined by mass spectroscopy, and the sequence of the target determined from the molecular weights of the fragments. Nucleic acids whose sequences can be determined include DNA or RNA in biological samples such as patient biopsies and environmental samples. Probes may be fixed to a solid support such as a hybridization chip to facilitate automated molecular weight analysis and identification of the target sequence.
摘要翻译：本发明涉及用于检测和测序靶核酸序列，批量修饰的核酸探针和可用于这些方法的探针阵列的方法，以及含有这些探针的试剂盒和系统。有用的方法包括将代表靶的互补或同源序列的核酸或核酸与核酸探针阵列进行杂交。这些探针包括在单链部分内的单链部分，任选的双链部分和可变序列。该组的杂交核酸的分子量可以通过质谱法测定，并且靶标的序列由片段的分子量确定。可以确定其序列的核酸包括生物样品中的DNA或RNA，例如患者活组织检查和环境样品。探针可以固定在固体支持物例如杂交芯片上以促进自动化分子量分析和靶序列的鉴定。

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